Allele Dmel\Mcm6^K1214

General Information
Symbol	Dmel\Mcm6^K1214	Species	D. melanogaster
Name		FlyBase ID	FBal0004303
Feature type	allele	Associated gene	Dmel\Mcm6
Also Known As	fs(1)K1214

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Komitopoulou et al., 1988)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:6,570,565..6,570,565 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=M676K\|Mcm6-PA reported_pr_change=M676K na_change=T6570565A (Schwed et al., 2002)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: M676K. (Schwed et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (with Mcm6²) (Schwed et al., 2002) female sterile (with Mcm6³) (Schwed et al., 2002) female sterile (with Mcm6⁴) (Schwed et al., 2002) female sterile (with Mcm6⁵) (Schwed et al., 2002) female sterile \| recessive (Schwed et al., 2002, ) female sterile soma-dependent (Perrimon and Gans, 1983) viable (Schwed et al., 2002) viable (with Mcm6²) (Schwed et al., 2002) viable (with Mcm6³) (Schwed et al., 2002) viable (with Mcm6⁴) (Schwed et al., 2002) viable (with Mcm6⁵) (Schwed et al., 2002) viable \| reduced
Phenotype Manifest In
	chorion (with Mcm6²) (Schwed et al., 2002) chorion (with Mcm6³) (Schwed et al., 2002) chorion (with Mcm6⁴) (Schwed et al., 2002) chorion (with Mcm6⁵) (Schwed et al., 2002) chorion \| maternal effect (Schwed et al., 2002) dorsal appendage dorsal appendage \| maternal effect (Schwed et al., 2002) egg \| maternal effect (Schwed et al., 2002)
Detailed Description
	Statement Reference Eggs laid by homozygous females are flaccid with thin, fragile eggshells (including flimsy dorsal appendages). Mutant follicle cells in mitotic and endocycles appear normal. However, most stage 10B mutant follicle cells have no detectable BrdU incorporation, while a few have faint or nearly wild-type incorporation. BrdU incorporation is mosaic among cells within an egg chamber. The dorsal-anterior cells most closely apposed to the oocyte nucleus and follicle cells in the posterior of the egg chamber most often have detectable BrdU incorporation. Inappropriate genomic BrdU incorporation is also seen in stage 10B (labelling is seen in other parts of the nucleus as well as the chorion loci). There is no significant difference in DNA content between wild-type and homozygous follicle cells. (Schwed et al., 2002) Morphological defects: substantial underproduction and disruption of the endochorion, correlated with the underproduction of 6 major chorion protein gene products: Cp15, Cp16, Cp18, Cp19, Cp36 and Cp38. (Komitopoulou et al., 1988) Viability and fecundity of homozygous females reduced; sterility virtually complete, but a few progeny escape. Dorsal appendages of chorion short and thin.
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Mcm6² (Schwed et al., 2002) Mcm6³ (Schwed et al., 2002) Mcm6⁴ (Schwed et al., 2002) Mcm6⁵ (Schwed et al., 2002)
Rescued by	Mcm6^K1214 is rescued by Mcm6^{Ubi-p63E.T:Zzzz\FLAG} (Schwed et al., 2002)
Comments
Stocks ( 2 )
Bloomington	4322 Mcm6^K1214 v²⁴/FM3
Kyoto	107688 Mcm6^K1214 v²⁴/FM3
Notes on Origin
Discoverer

Comments
	Ovaries derived from homozygous females show reduced incorporation of BrdU compared to wild-type, and labeling is mosaic in intensity among the follicle cells. (Calvi et al., 1998) All major chorion proteins underproduced owing to a defect in amplification of Cp genes on both the X and chromosome 3. (Orr et al., 1984)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	fs(1)K1214 (Schwed et al., 2002, Cernilogar et al., 2001, Calvi et al., 1998, Perrimon et al., 1986, Perrimon and Gans, 1983) fs(1)K1214¹ (Komitopoulou et al., 1988, ) mcm6^fs(1)k1214 (Claycomb et al., 2004) MCM6^K1214 (Schwed et al., 2002) Mcm6^K1214 (Christensen et al., 2008.6.11) mcm6^K1214 (Gause et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Gause et al., 2010, Mol. Cell. Biol. 30(20): 4940--4951 Dosage-sensitive regulation of Cohesin chromosome binding and dynamics by Nipped-B, Pds5, and Wapl. [FBrf0211896] Claycomb et al., 2004, Dev. Cell 6(1): 145--155 Gene amplification as a developmental strategy: isolation of two developmental amplicons in Drosophila. [FBrf0167486] Schwed et al., 2002, Mol. Biol. Cell 13(2): 607--620 Drosophila minichromosome maintenance 6 is required for chorion gene amplification and genomic replication. [FBrf0145110] Cernilogar et al., 2001, Dev. Genes Evol. 211(12): 573--580 Drosophila vitelline membrane cross-linking requires the fs(1)Nasrat, fs(1)polehole and chorion genes activities. [FBrf0144809] Calvi et al., 1998, Genes Dev. 12(5): 734--744 Cell cycle control of chorion gene amplification. [FBrf0101877] Komitopoulou et al., 1988, Dev. Genet. 9: 37--48 Structural and biochemical studies on four sex-linked chorion mutants of Drosophila melanogaster. [FBrf0047850] Perrimon et al., 1986, Genetics 113: 695--712 X-linked female-sterile loci in Drosophila melanogaster. [FBrf0044470] Orr et al., 1984, Proc. Natl. Acad. Sci. U.S.A. 81: 3773--3777 Mutants suppressing in trans chorion gene amplification in Drosophila. [FBrf0041663] Perrimon and Gans, 1983, Dev. Biol. 100: 365--373 Clonal analysis of the tissue specificity of recessive female sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation. [FBrf0039213]
Personal communication to FlyBase	Christensen et al., 2008.6.11, Isolation and characterization of Df(1)BSC535. Isolation and characterization of Df(1)BSC535. [FBrf0204976]

Allele Dmel\Mcm6K1214

Allele Dmel\Mcm6^K1214