FB2025_01 , released February 20, 2025
Allele: Dmel\fs(1)N12
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General Information
Symbol
Dmel\fs(1)N12
Species
D. melanogaster
Name
FlyBase ID
FBal0004463
Feature type
allele
Associated gene
Dmel\fs(1)N
Associated Insertion(s)
Carried in Construct
Also Known As
fs(1)N211, fs(1)Nas211
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Degelmann et al., 1990)
Progenitor genotype
Cytology
Description

Amino acid replacement: P830L.

(Jimenez et al., 2002)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:1,325,293..1,325,293 [+]
Nucleotide change:

C1325293T

Amino acid change:

P830L | fs(1)N-PA

Reported amino acid change:

P830L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Jimenez et al., 2002)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      fs(1)N14/fs(1)N12 mothers produce embryos with a terminal phenotype.

      (Jimenez et al., 2002)

      Embryos derived from homozygous females show a terminal phenotype.

      (Furriols et al., 1998)

      Deletion of anterior and posterior structures.

      (Perrimon et al., 1991)

      Failure to differentiate cuticular structures derived from the anterior and posterior regions of the egg.

      (Degelmann et al., 1990)

      A hole is seen in the blastoderm layer below the pole cells in embryos. The ventral furrow is extended posteriorly. Segments A8 to the telson are deleted. Segments A5 to A7 are expanded. Some twisting of the germband is seen. Labral and acron-derived structures are deleted. There is cell death in the head and tail region.

      (Ambrosio et al., 1989)

      A single tor11D allele variably suppresses the phenotype caused by fs(1)N12 in embryos derived from females homozygous for fs(1)N12 and heterozygous for tor11D.

      (Casanova and Struhl, 1989)

      Embryos display a tor phenotype.

      (Perrimon et al., 1986)

      Eggs are laid but collapse on deposition.

      (Mohler and Carroll, 1984)

      Homozygous fs(1)N12 females produce embryos with defects in anterior and posterior development (Degelmann, Hardy, Perrimon and Mahowald, 1986; Degelmann, Hardy and Mahowald, 1990). Head involution fails; anterior endodermal derivatives are deficient and the absence of pharyngeal musculature causes collapse of the cephalopharyngeal apparatus. Embryos lack all posterior endodermal derivatives as well as structures characteristic of abdominal segments 8 to 10 usually, but have six abdominal denticle belts, and occasionally partial seventh or seventh and eighth. In addition, posterior midgut, hindgut, Malpighian tubules, filzkorper, spiracles, anus, anal pad and anal tuft absent; lateral tracheal trunks end blindly, usually in the sixth segment; posterior disorganization of CNS seen. Pole cells form abnormally and seldom migrate to form the gonad; posterior blastoderm nuclei fail to cellularize and the centrosome position vis a vis the blastoderm nuclei erratic; remarkably free of microtubules. Six rather than seven stripes of ftz transcription seen in blastoderm, with the sixth stripe wider than normal and separated from the fifth by a wider-than-normal space. At gastrulation neither posterior midgut invagination nor proctodeal invagination occur normally.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      fs(1)N12 is a suppressor of embryonic segment phenotype of tsltor.PF

      (Furriols et al., 1998)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos derived from fs(1)N12 females carrying tsltor.PF show no rescue of the fs(1)N12 phenotype and do not show deletions of the middle segments ("splice" phenotype seen in wild-type embryos carrying tsltor.PF).

      (Furriols et al., 1998)

      In double mutants Pc3, fs(1)N12, exu1, fs(1)N12, vas1, fs(1)N12 and BicD, fs(1)N12 all abdominal segments are transformed to the eighth abdominal segment. Double homozygous females fs(1)N12, trk4 and fs(1)N12, fs(1)M31901 produce embryos with the terminal cuticle phenotype.

      (Degelmann et al., 1990)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      fs(1)N14

      (Jimenez et al., 2002)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      Engstrom.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      References (15)