FlyBase Allele Report: Dmel\γTub37C[1]

General Information

Symbol

Dmel\γTub37C¹

Species

D. melanogaster

Name

FlyBase ID

FBal0004702

Feature type

allele

Associated gene

Dmel\γTub37C

Associated Insertion(s)

Carried in Construct

Also Known As

fs(2)TW1¹

Key Links

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point_mutation

2L:19,184,362..19,184,362 [-]

Nucleotide change:

G19184362A

Reported nucleotide change:

G1027A|FBrf0105422

Amino acid change:

W352term | gammaTub37C-PA; W352term | gammaTub37C-PB

Reported amino acid change:

W352term

(Llamazares et al., 1999, Tavosanis, 1998.8.1, Tavosanis, 1998.11.24)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Nucleotide substitution: G?A.

(Llamazares et al., 1999)

Amino acid replacement: W352term.

(Llamazares et al., 1999, Tavosanis, 1998.11.24)

Nucleotide coordinates are numbered according to the coding region.

(Tavosanis, 1998.11.24)

Nucleotide substitution: G1027A.

(Tavosanis, 1998.11.24)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Tavosanis et al., 1997)

female sterile | recessive

(Wright, 1996)

lethal - all die during embryonic stage | recessive

(Tavosanis et al., 1997)

male fertile

(Wright, 1996)

some die during embryonic stage | recessive

(Tavosanis et al., 1997)

Phenotype Manifest In

aster | embryonic stage

(Llamazares et al., 1999)

embryo & mitosis & nuclear chromosome

(Llamazares et al., 1999)

meiotic metaphase I

nucleus (with γTub37C³)

(Delanoue and Davis, 2005)

oocyte (with γTub37C³)

(Wilson and Borisy, 1998)

polar body nucleus

(Llamazares et al., 1999)

polar body nucleus (with γTub37C³)

(Wilson and Borisy, 1998)

spindle

(Tavosanis et al., 1997)

spindle & oocyte (with γTub37C³)

(Jang et al., 2005)

spindle | embryonic stage

(Llamazares et al., 1999)

Detailed Description

Statement

Reference

γTub37C¹/γTub37C³ embryos show defects in anchoring of nuclei to the surface but show no defects in apical mRNA localization.

(Delanoue and Davis, 2005)

γTub37C¹/γTub37C³ females have disorganised meiotic spindles in the oocyte.

(Jang et al., 2005)

The microtubule cytoskeleton is overall properly organised during oogenesis in homozygous female mutants.

(Tavosanis and Gonzalez, 2003)

Embryos derived from homozygous females do not contain polar bodies. Mitotic spindles of the kind found in wild-type embryos are not seen. Most of these embryos contain one or sometimes two spindle-like structures together with a few other microtubule arrays. Both unfertilised laid eggs and fertilised eggs derived from homozygous females show these phenotypes. The pole-to-pole distance of the spindle-like structures is variable, sometimes reaching over 40μm in length. The spindle-like structures are thicker than a wild-type meiosis-I spindle and contain variable amounts of chromatin. Chromosomes can be found scattered along the spindle as well as located at the equator. Chromosome condensation is variable although it is high. Asters are not seen at the poles of the spindle-like structures.

(Llamazares et al., 1999)

Eggs derived from γTub37C³/γTub37C¹ females do not show polar body structures, but do have robust polar spindles. γTub37C³/γTub37C¹ females yield less than 5% of the activated oocytes obtained from wild-type ovaries, when the oocytes are activated in vitro.

(Wilson and Borisy, 1998)

The chromosome arrangement at metaphase-I arrest females is severely disrupted, the chromosomes are arranged randomly. 80% of meiotic figures are abnormal in oocytes. Defect is due to failure to organise the meiotic spindle. Cleavage divisions are also impaired, embryos display abnormal patterns of chromatin distribution, microtubule organisation is also extremely abnormal. Embryonic development is arrested before the syncytial blastoderm stage. Defects are due to a requirement for γTub37C function during embryogenesis. Females can be rescued to fertility by γTub37C^+t7.2.

(Tavosanis et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

γTub23C^PI, γTub37C¹ has female sterile phenotype

(Tavosanis and Gonzalez, 2003)

γTub23C^PI, γTub37C¹ has decreased fecundity | female phenotype

(Tavosanis and Gonzalez, 2003)

γTub23C^PI, γTub37C¹ has decreased cell number | third instar larval stage phenotype

(Tavosanis and Gonzalez, 2003)

γTub23C^PI, γTub37C¹ has decreased cell number | prepupal stage phenotype

(Tavosanis and Gonzalez, 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

γTub37C¹ has phenotype, enhanceable by E(γTub37C¹)¹

(Bloomington Drosophila Stock Center, 1997.12.5)

Other

Statement

Reference

γTub23C^PI, γTub37C¹ has germline cell | female phenotype

(Tavosanis and Gonzalez, 2003)

γTub23C^PI, γTub37C¹ has mitotic cell cycle phenotype

(Tavosanis and Gonzalez, 2003)

γTub23C^PI, γTub37C¹ has ovary phenotype

(Tavosanis and Gonzalez, 2003)

γTub23C^PI, γTub37C¹ has spindle & germ cell | female phenotype

(Tavosanis and Gonzalez, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous γTub23C^PI/γTub37C¹ mutant females are agametic with rudimentary ovaries. The addition of γTub23C^+tLa or γTub37C^+t7.2 rescues these phenotypes. In homozygous γTub23C^PI/γTub37C¹ mutant embryos, germ cells form, migrate and populate the gonads as wild-type. Ovaries of mutant second instar larvae are also indistinguishable from wild-type. By the third instar larval stage the number of germ cells is slightly reduced. At prepupa stage, germ cells are found scattered throughout the mutant ovary, their total number is dramatically reduced. From this stage on germ cells degenerate and steadily disappear from the mutant, so that in adult ovaries the germaria are empty. In homozygous γTub23C^PI/γTub37C¹ mutant third instar larval females, half of the germ-line mitotic figures have centrosomes that are not aligned along the axis of the condensed chromosome plate. The condensation of the chromosomes is too high. Correspondingly, mitotic spindles are composed of shirt, often disorganised, microtubules associated with the chromosomes.

(Tavosanis and Gonzalez, 2003)

Does not interact with alleles of sub.

(Giunta et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

γTub37C¹³⁹

(Schnorrer et al., 2002)

Rescued by

γTub37C¹ is rescued by γTub37C^+t7.2

(Tavosanis and Gonzalez, 2003)

γTub37C¹ is rescued by γTub37C^+t7.2

(Tavosanis et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3188

E(γTub37C¹)¹ γTub37C¹ pr¹ Ddc^ts1/CyO

Kyoto

106902

E(γTub37C¹)¹ γTub37C¹ pr¹ Ddc^ts1/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF081253

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAC35844

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

fs(2)TW1.1

(Tavosanis, 1998.11.24)

fs(2)TW1¹

(Tavosanis and Gonzalez, 2003, Llamazares et al., 1999, Tavosanis et al., 1997, Wright, 1996)

γ-Tub37C¹

(Jang et al., 2005)

γTub37C¹

(Delanoue and Davis, 2005)

γTub37CD^TW1.1

(Wilson and Borisy, 1998)

Name Synonyms

Secondary FlyBase IDs

References (12)

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