There is an mdg3 retrotransposon inserted in position S2929 that introduces a stop codon in the 27th cadherin domain. It also contains additional rearrangements in the first intron and an insertion in the 33rd cadherin domain.
As for ftG with an additional rearrangement that has not yet been fully characterized.
hyperplasia (with ft4)
hyperplasia (with ft8)
short lived (with ft1)
size defective (with ft8), with ftΔD.Tag:V5
size defective | larval stage (with ft8)
size defective | third instar larval stage (with ft8), with Scer\GAL4Act5C.PI, ftΔICD.UAS.Tag:HA
size defective | third instar larval stage (with ft8), with Scer\GAL4da.G32, ftΔICD.UAS.Tag:HA
abdomen | pupal stage (with ft8)
eye disc | pupal stage (with ft8)
macrochaeta (with ft8)
mesothoracic tergum & chaeta | somatic clone
microchaeta (with ft8)
wing (with ft8), with ftΔD.Tag:V5
wing (with ft8), with ftΔF.Tag:V5
wing & chaeta | somatic clone
wing disc (with ft8), with Scer\GAL4Act5C.PI, ftΔICD.UAS.Tag:HA
wing disc (with ft8), with Scer\GAL4da.G32, ftΔICD.UAS.Tag:HA
wing disc | pupal stage (with ft8)
ftG-rv/ftG-rv eyes (created by the EGUF method in otherwise heterozygous animals) contain significantly increased number of interomatidial cells (IOC) in the pupal retina compared to wild-type, the size and shape of adult eyes is also affected.
ftG-rv/ft8 transheterozygotes rarely survive to third instar larval stage (but never to adulthood) and the survivors display decreased number of photoreceptor cells in the eye disc and overgrown wing discs compared to wild-type.
ftG-rv homozygous mutant embryos display a significant increase in the frequency of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage.
ftG-rv/ft8; ftT:SV5\V5 wings are normal; ftG-rv/ft8; ftΔD.T:SV5\V5 wings are slightly overgrown, rounder and have decreased spacing between the crossveins compared to controls; ftG-rv/ft8; ftΔF.T:SV5\V5 wings are not enlarged but have greatly reduced spacing between the crossveins.
ft8/ftG-rv and ft8/ft61 imaginal discs are larger and have more folds than controls.
ftG-rv mutant MARCM clones contain 5-7 cells per clone, compared to 2-3 cells in wild-type clones. These clones contain differentiated absorptive enterocytes and secretory enteroendocrine cells indicating that intestine stem cell differentiation continues as in wild-type.
ftG-rv homozygous mutant clones generated in the male germline develop normally. 16 cells are observed per cyst, and cell size and morphology are indistinguishable from neighbouring control cells.
The wing discs of ftG-rv/ft8 larvae show extensive tissue overgrowth during the late third instar.
Expression of ftΔICD.Scer\UAS.T:Ivir\HA1, under the control of either Scer\GAL4Act5C.PI or Scer\GAL4da.G32, enhances the wing disc tissue overgrowth phenotype of ftG-rv/ft8 mutants.
Large ftG-rv clones in the distal region of the wing blade between L2 and L3 show planar cell polarity defects.
The abdomens of ftG-rv/ft8 mutants have fairly normal morphology at the pharate stages although they have a characteristic swirl of hairs that have lost their normal polarity.
Mutant wing and eye discs exhibit an overgrowth phenotype. ftG-rv homozygous mutant eyes (created as clones) are slightly larger than normal with duplicated bristle cells and severe morphological defects. The distribution of cell cycle phases and cell size in ftG-rv mutants are indistinguishable from wild-type.
About 50% of ommatidia within somatic clones in the adult eye frequently have reversed dorsal ventral polarity. Occasional reversals of polarity are also seen in wild-type ommatidia bordering the polar side of the mutant tissue. When homozygous mutant clones are made specifically in the equatorial R3/R4 precursor, almost all (96%) of the consequent ommatidia are in the reverse polarity. When clones are made specifically in the polar R3/R4 precursor, all of the consequent ommatidia are in the wild-type polarity. The mutant photoreceptor precursor eventually becomes an R4 photoreceptor 98% of the time (50% in wild-type).
ftG-rv/Df(2L)sc19-1 larvae reach the pupal stage at 7-9 days and show hyperplastic discs. ftk07918/ftG-rv larvae show a delay in pupariation of 1-2 days and have a maximal disc size similar to that of ftk07918 homozygotes. Wing discs from ft4/ftG-rv larvae are larger, they pupate at 7-9 days and die as pupae. Somatic wing clones of homozygous ftG-rv in a M(2)24F1 background tend to fill one or several intervein sectors but a fraction do cross the dorsal ventral boundary. The clones tend to fill proximal regions of the wing. The distal borders of clones are rounded, and are smooth in contrast with the indented borders found in control clones. Inn all of these features ftk07918 and ftG-rv are more extreme than ft4. Trichome density in clones is higher than wild-type clones - about 1.7 times that of wild-type, indicating that cell size is smaller than wild-type. Somatic clones in the notum (of homozygous ft4 in a M(2)24F1 background) are large, with more cells than control clones, leading to an increase in the total notum surface. They contain many more chaetae (1.6 times wild-type) and higher cell density (1.4 times). Similar deviations occur in the legs and head capsule. tergite clones however shoe normal patterns of pigment chaetae and trichomes.
Does not show G phenotype. Does not exaggerate ft. Lethal in combination with G. RK2.
ftG-rv has hyperplasia phenotype, enhanceable by MycUAS.cZa/Scer\GAL4hh.PU
ft8/ftG-rv has size defective | third instar larval stage phenotype, enhanceable by dsUAO71
ft8/ftG-rv has partially lethal - majority die phenotype, enhanceable by ex[+]/exe1
ftG-rv has increased cell number | cell non-autonomous phenotype, enhanceable by Scer\GAL4dpp.blk1/RafUAS.F179
ftG-rv has increased cell number phenotype, enhanceable by rhoUAS.cdCa/Scer\GAL4dpp.blk1
ftG-rv/ft1 has short lived phenotype, enhanceable by Gug11/Gug[+]
ft8/ftG-rv has lethal phenotype, suppressible by wtsUAS.Tag:MYC/Scer\GAL4αTub84B.PL
ft8/ftG-rv has planar polarity defective | third instar larval stage phenotype, suppressible by wtsUAS.Tag:MYC/Scer\GAL4αTub84B.PL
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has planar polarity defective | adult stage phenotype, suppressible by ftTag:V5
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has planar polarity defective | adult stage phenotype, suppressible | partially by ftΔICD.Tag:V5
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has planar polarity defective | adult stage phenotype, suppressible | partially by ft::Hsap\FAT4Tag:V5
ftΔF.Tag:V5, ft8/ftG-rv has planar polarity defective | adult stage phenotype, suppressible | partially by ft::Hsap\FAT4Tag:V5
ft8/ftG-rv has planar polarity defective phenotype, suppressible | partially by ftλftICD.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv has lethal phenotype, suppressible by Scer\GAL4Tub.PU/wtsUAS.Tag:MYC
ft8/ftG-rv has size defective phenotype, suppressible by Scer\GAL4Tub.PU/wtsUAS.Tag:MYC
ft8/ftG-rv has planar polarity defective | pupal stage phenotype, suppressible | partially by appe6
ft8/ftG-rv has increased cell number | somatic clone phenotype, suppressible by ykiB5
ftG-rv has increased cell number phenotype, suppressible by RafK497M.UAS/Scer\GAL4dpp.blk1
ft8/ftG-rv has hyperplasia phenotype, suppressible | partially by wgspd-fg
ftG-rv has cell polarity defective | somatic clone phenotype, suppressible | partially by dsUAO71
ftG-rv has visible | adult stage phenotype, non-suppressible by ZyxΔ41/ZyxΔ41
ft8/ftG-rv has size defective | larval stage phenotype, non-suppressible by Scer\GAL4αTub84B.PL/Fbxl7UAS.Tag:FLAG
ft8/ftG-rv has lethal phenotype, non-suppressible by Scer\GAL4αTub84B.PL/Fbxl7UAS.Tag:FLAG
ft8/ftG-rv has planar polarity defective | adult stage phenotype, non-suppressible by wtsUAS.Tag:MYC/Scer\GAL4αTub84B.PL
ft8/ftG-rv has planar polarity defective phenotype, non-suppressible by Scer\GAL4Act.PU/dsUAS.cTa
ft8/ftG-rv has planar polarity defective phenotype, non-suppressible by Scer\GAL4Tub.PU/wtsUAS.Tag:MYC
ftG-rv is an enhancer | somatic clone of planar polarity defective phenotype of Scer\GAL4dpp.blk1, fzUAS.cAa
ftG-rv is an enhancer of visible phenotype of Scer\GAL4en-e16E, armUAS.cWa
ftG-rv is an enhancer of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, armUAS.cWa
ftG-rv/ftG-rv is a non-enhancer of size defective phenotype of Dlishc
ftG-rv is a suppressor of visible phenotype of HScer\UAS.cMa/HUAS.cMa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
ftG-rv is a suppressor of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, shgi.UAS.Tag:SS(aos),Tag:MYC
ftG-rv is a suppressor of visible phenotype of Scer\GAL4en-e16E, shgi.UAS.Tag:SS(aos),Tag:MYC
ftG-rv is a non-suppressor of lethal | pupal stage phenotype of Cskj1D8/CskS030003
cno2, ftG-rv/ft[+] has neuroanatomy defective | embryonic stage phenotype
Scer\GAL4hh.PU, MycUAS.cZa, ftG-rv has increased cell size phenotype
Scer\GAL4dpp.blk1, ftG-rv, RafK497M.UAS has increased cell death phenotype
ftG-rv has eye | somatic clone phenotype, enhanceable by rhoUAS.cdCa/Scer\GAL4ey.PH
ftG-rv has wing disc | cell non-autonomous phenotype, enhanceable by Scer\GAL4dpp.blk1/RafUAS.F179
ftG-rv has eye disc | cell non-autonomous phenotype, enhanceable by Scer\GAL4dpp.blk1/RafUAS.F179
ftG-rv has eye disc phenotype, enhanceable by rhoUAS.cdCa/Scer\GAL4dpp.blk1
ftG-rv has wing disc phenotype, enhanceable by rhoUAS.cdCa/Scer\GAL4dpp.blk1
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has wing phenotype, non-enhanceable by ftΔEGF.Tag:V5
ftG-rv has wing disc phenotype, non-enhanceable by RafK497M.UAS/Scer\GAL4dpp.blk1
ftG-rv has interommatidial bristle | supernumerary | somatic clone phenotype, non-enhanceable by RafK497M.UAS/Scer\GAL4ey.PH
ftG-rv has interommatidial cell | supernumerary | somatic clone | pupal stage phenotype, suppressible | partially by ZyxΔ41/ZyxΔ41
ft8/ftG-rv has wing disc | third instar larval stage phenotype, suppressible by wtsUAS.Tag:MYC/Scer\GAL4αTub84B.PL
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has wing hair phenotype, suppressible by ftTag:V5
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has abdomen phenotype, suppressible by ftTag:V5
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has wing hair phenotype, suppressible | partially by ftΔICD.Tag:V5
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has abdomen phenotype, suppressible | partially by ftΔICD.Tag:V5
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has wing hair phenotype, suppressible | partially by ft::Hsap\FAT4Tag:V5
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has abdomen phenotype, suppressible by ft::Hsap\FAT4Tag:V5
ftΔF.Tag:V5, ft8/ftG-rv has wing hair phenotype, suppressible | partially by ft::Hsap\FAT4Tag:V5
ft8/ftG-rv has wing disc | pupal stage phenotype, suppressible by appe6
ft8/ftG-rv has eye disc | pupal stage phenotype, suppressible by appe6
ftG-rv has eye disc phenotype, suppressible by RafK497M.UAS/Scer\GAL4dpp.blk1
ftG-rv has eye | somatic clone phenotype, suppressible by RafK497M.UAS/Scer\GAL4ey.PH
ftG-rv has ommatidium | somatic clone phenotype, suppressible | partially by dsUAO71
ftG-rv has photoreceptor cell R3 | somatic clone phenotype, suppressible | partially by dsUAO71
ftG-rv has photoreceptor cell R4 | ectopic | somatic clone phenotype, suppressible | partially by dsUAO71
ft8/ftG-rv has imaginal disc phenotype, non-suppressible by Scer\GAL4αTub84B.PL/Fbxl7UAS.Tag:FLAG
ft8/ftG-rv has wing hair phenotype, non-suppressible by wtsUAS.Tag:MYC/Scer\GAL4αTub84B.PL
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has wing phenotype, non-suppressible by ftΔEGF.Tag:V5
ftΔD.Tag:V5, ft8/ftG-rv has wing phenotype, non-suppressible by ft::Hsap\FAT4Tag:V5
ftG-rv has wing disc phenotype, non-suppressible by RafK497M.UAS/Scer\GAL4dpp.blk1
ftG-rv has interommatidial bristle | supernumerary | somatic clone phenotype, non-suppressible by RafK497M.UAS/Scer\GAL4ey.PH
ftG-rv/ft[+] is an enhancer of eye photoreceptor cell phenotype of Gug75QN.UAS, Scer\GAL4ninaE.PT
ftG-rv/ft[+] is an enhancer of crossvein phenotype of fjt14.Tag:Golgi(hGALNT3)
ftG-rv/ft[+] is an enhancer of wing phenotype of fjt14.Tag:Golgi(hGALNT3)
ftG-rv is an enhancer | somatic clone of wing hair | anterior | P-stage phenotype of Scer\GAL4dpp.blk1, fzUAS.cAa
ftG-rv is an enhancer of wing phenotype of Scer\GAL4en-e16E, armUAS.cWa
ftG-rv is an enhancer of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, armUAS.cWa
ftG-rv/ftG-rv is a non-enhancer of abdominal tergite marginal bristle phenotype of Dlishc
ftG-rv is a suppressor of eye phenotype of HScer\UAS.cMa/HUAS.cMa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
ftG-rv is a suppressor of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, shgi.UAS.Tag:SS(aos),Tag:MYC
ftG-rv is a suppressor of wing phenotype of Scer\GAL4en-e16E, shgi.UAS.Tag:SS(aos),Tag:MYC
cno2, ftG-rv/ft[+] has RP2 motor neuron | embryonic stage phenotype
Scer\GAL4αTub84B.PL, ft8/ftG-rv, wtsUAS.Tag:MYC has abdomen phenotype
Scer\GAL4Tub.PU, ft8/ftG-rv, wtsUAS.Tag:MYC has wing vein phenotype
Scer\GAL4ey.PH, ftG-rv, RafK497M.UAS has eye | somatic clone phenotype
The lethality of ftG-rv homozygotes is suppressed by the additional homozygosity for Dlishc, but adults are recovered only at about one third of the expected frequency.
Additional homozygosity for ftG-rv does not exacerbate the small wing area phenotype and mild abdomen hair orientation defects observed in Dlishc single homozygotes.
The supernumerary inter-ommatidial cells in the pupal retina phenotype characteristic for ftG-rv/ftG-rv eyes (created by the EGUF method in otherwise heterozygous animals) can be partially suppressed by combination with ZyxΔ41/ZyxΔ41 but this does not affect the size of the mutant adult eyes.
The low rate of survival of ftG-rv/ft8 transheterozygotes to third instar larval stage (and never to adulthood), the loss of photoreceptor cells in the eye discs or the overgrowth of the wing disc cannot be suppressed by combination with ZyxΔ41/ZyxΔ41.
Scer\GAL4αTub84B.PL-mediated expression of Fbxl7Scer\UAS.T:Zzzz\FLAG does not suppress ft8/ftG-rv phenotypes.
Flies expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL rescue the lethality and third instar larval wing disc overgrowth phenotypes seen in ft8/ftG-rv mutants, but the wing hair phenotypes are still present. Rather than pointing distally as in wild type, the wing hairs in these flies are misoriented and swirling patterns can be observed. A strong planar cell polarity defect is also seen in the abdominal hairs and the wing anterior-posterior crossvein distance is reduced.
Expression of ftT:SV5\V5 fully rescues the planar cell polarity defects seen in ft8/ftG-rv mutant wings and abdomens expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL. The reduced anterior-posterior crossvein distance phenotype is also suppressed.
Flies expressing ftΔEGF.T:SV5\V5 (co-expressed with wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL) in a ft8/ftG-rv mutant background do not exhibit any wing phenotypes compared to flies expressing wtsScer\UAS.T:Hsap\MYC alone.
Expression of ftΔICD.T:SV5\V5 partially rescues the planar cell polarity defects seen in ft8/ftG-rv mutant wings and abdomens expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL. The reduction in anterior-posterior crossvein distance appears to be partially rescued, although crossveins are often incomplete, making it difficult to calculate. The abdominal and wing hair polarity defects are also partially suppressed.
Expression of btl::ftftICD.T:Ivir\HA1,T:λ\cI-DD under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals rescues disc overgrowth, allows eclosion and improves hair planar cell polarity defects in the wing and abdomen.
Expression of dsScer\UAS.cTa under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals does not detectably improve hair planar cell polarity defects in the abdomen.
dmScer\UAS.cZa overexpression clones (under the control of Scer\GAL4hh.PU) found in the posterior of the wing disc strongly enhance the proliferative activity of ftG-rv mutant cells. In addition these cells are larger in dmScer\UAS.cZa clones than in a wild-type background.
The lethality and overgrowth phenotypes of ftG-rv/ft8 animals are rescued by Scer\GAL4tub.PU-mediated overexpression of wtsScer\UAS.T:Hsap\MYC. However, these animals still show obvious planar cell polarity phenotypes in multiple tissues.
The disc overgrowth phenotypes of ft8/ftG-rv pupae are suppressed by appe6 and the abdominal planar cell polarity (PCP) and lethality phenotypes are reduced in the double mutants compared to the ft8/ftG-rv single mutant. The double mutant adults have PCP defects in proximal portions of the wing, but not in the distal wing.
ftG-rv allows recovery of Df(1)su(s)R194/+ clones in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals).
Nearly all ft8 ykiB5/ftG-rv mutant animals develop into adults, although they do not hatch from the pupal case as they have some leg defects and head overgrowths. In contrast, all ft8/ftG-rv mutant animals die during the early stages of pupal development and show severely overgrown imaginal disc derivatives. This indicates that ykiB5 suppresses the overgrowth phenotypes of ft8/ftG-rv mutant imaginal discs.
When rhoScer\UAS.cdCa is driven by Scer\GAL4dpp.blk1 in a ftG-rv background a significant enhancement is seen in the ftG-rv overgrowth phenotype in the Scer\GAL4dpp.blk1 expression territory. When phlScer\UAS.F179 is driven by Scer\GAL4dpp.blk1 in a ftG-rv background a significant enhancement is seen in the ftG-rv overgrowth phenotype, throughout the whole imaginal disc. When phlK497M.Scer\UAS is driven by Scer\GAL4dpp.blk1 in a ftG-rv background a suppression is seen in the ftG-rv overgrowth phenotype in the eye disc, but no effect is seen in the wing disc. An increase in cell non-autonomous cell death is seen in these discs. When rhoScer\UAS.cdCa is driven by Scer\GAL4ey.PH in ftG-rv mutant eyes, significantly enlarged eyes are seen. When phlK497M.Scer\UAS is driven by Scer\GAL4ey.PH in ftG-rv mutant eyes, eyes are significantly smaller than wild-type. However eye bristle duplications are still seen.
Reduction in the distance between anterior and posterior cross-veins in fjt14.T:Hsap\GALNT3 flies is enhanced by ftG-rv/+.
fzScer\UAS.cAa; Scer\GAL4dpp.blk1 flies have a zone extending anterior to the dpp expression domain in which wing hairs point away from the fz overexpressing cells. When such wings have somatic clones of ftG-rv in the anterior of the wing, this zone enlarges to fill the clone.
When ftG-rv/dsUAO71 homozygous mutant clones are made specifically in the equatorial R3/R4 precursor, most (64%) of the consequent ommatidia are in the reverse polarity. When clones are made specifically in the polar R3/R4 precursor, almost all (97.5%) of the consequent ommatidia are in the wild-type polarity. The mutant photoreceptor precursor eventually becomes an R4 photoreceptor 80% of the time (50% in wild-type).
Expression of ft::Hsap\FAT4T:SV5\V5 partially rescues the planar cell polarity defects seen in ft8/ftG-rv mutant wings expressing wtsScer\UAS.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL. The abdominal hair defects are completely rescued and the reduction in anterior-posterior crossvein distance is partially suppressed.
Expression of one copy of ft::Hsap\FAT4T:SV5\V5 fails to suppress the wing overgrowth phenotypes seen in ft8/ftG-rv mutant flies expressing one copy of ftΔD.T:SV5\V5.
Expression of one copy of ft::Hsap\FAT4T:SV5\V5 partially suppresses the wing crossvein spacing and hair polarity phenotypes seen in ft8/ftG-rv mutant flies expressing one copy of ftΔF.T:SV5\V5.
ft8/ftG-rv is rescued by ftΔA.Tag:V5
ft8/ftG-rv is rescued by ftΔB.Tag:V5
ft8/ftG-rv is rescued by ftΔC.Tag:V5
ft8/ftG-rv is rescued by ftP32.Tag:V5
ft8/ftG-rv is rescued by ftmIV.Tag:V5
ft8/ftG-rv is rescued by ftUAS.cMa/Scer\GAL4da.G32
ft8/ftG-rv is rescued by ftUASp.cSa/Scer\GAL4αTub84B.PL
ft8/ftG-rv is partially rescued by ftΔD.Tag:V5
ft8/ftG-rv is partially rescued by ftΔF.Tag:V5
ft8/ftG-rv is partially rescued by ftΔD.Tag:V5
ft8/ftG-rv is partially rescued by ftΔD.Tag:V5/ftΔF.Tag:V5
ft8/ftG-rv is partially rescued by ftΔE.Tag:V5
ft8/ftG-rv is partially rescued by ftΔF.Tag:V5
ft8/ftG-rv is partially rescued by ftmV.Tag:V5
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECD.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftΔECD2.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by ftUAS.cMa/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔN-1.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftΔECDΔN-2.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔN-4.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ9-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ8-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ7-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftΔECDΔ6-C.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ5-C.UAS
ft8/ftG-rv is partially rescued by ftΔECDΔ4-C.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ2-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ1-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftΔECDΔ5-6.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ1-3.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ3-4.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔECDΔ3-5.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftΔECDΔ1-5.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔN-1.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔN-2.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔN-4.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔN-5.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔN-6.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftΔ9-C.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔ8-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔ7-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔ6-C.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftΔ5-C.UAS/Scer\GAL4Act.PU
ft8/ftG-rv is partially rescued by Scer\GAL4Act.PU/ftΔICD.UAS.Tag:HA
ft8/ftG-rv is partially rescued by ftUAS.cMa/Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI
ft8/ftG-rv is partially rescued by ftUAS.cMa/Scer\GAL4en-e16E
ft8/ftG-rv is partially rescued by Scer\GAL4en-e16E/ftΔECD.UAS
ft8/ftG-rv is partially rescued by Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI/ftΔECD.UAS
ft8/ftG-rv is partially rescued by Scer\GAL4da.G32/ftΔECD.UAS
ft8/ftG-rv is partially rescued by ftUAS.cMa/Scer\GAL4da.G32
ft8/ftG-rv is not rescued by ftΔEGF.Tag:V5
ft8/ftG-rv is not rescued by ftΔICD.Tag:V5
ft8/ftG-rv is not rescued by ftICD.UAS.Tag:HA/Scer\GAL4Act.PU
ft8/ftG-rv is not rescued by Scer\GAL4Act.PU/ftΔECDΔN-5.UAS.Tag:HA
ft8/ftG-rv is not rescued by Scer\GAL4Act.PU/ftΔECDΔN-6.UAS.Tag:HA
ft8/ftG-rv is not rescued by Scer\GAL4Act.PU/ftΔECDΔICD.UAS.Tag:HA
ft8/ftG-rv is not rescued by Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI/ftΔICD.UAS.Tag:HA
ft8/ftG-rv is not rescued by Scer\GAL4da.G32/ftΔICD.UAS.Tag:HA
ft8/ftG-rv is not rescued by Scer\GAL4en-e16E/ftΔICD.UAS.Tag:HA
Expression of ftΔA.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. The rescued animals appear morphologically normal and only a negligible wing hair planar cell polarity phenotype is seen. The abdominal hair polarity phenotype is fully rescued. The intercellular ridges that run from anterior to posterior in the ft8/ftG-rv mutant posterior wings run proximally to distal in rescue animals, as is seen in wild type.
Expression of ftΔB.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. The rescued animals appear morphologically normal and only a negligible wing hair planar cell polarity phenotype is seen. The abdominal hair polarity phenotype is fully rescued. The intercellular ridges that run from anterior to posterior in the ft8/ftG-rv mutant posterior wings run proximally to distal in rescue animals, as is seen in wild type.
Expression of ftΔC.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. The rescued animals appear morphologically normal and only a negligible wing hair planar cell polarity phenotype is seen. The abdominal hair polarity phenotype is fully rescued. The intercellular ridges that run from anterior to posterior in the ft8/ftG-rv mutant posterior wings run proximally to distal in rescue animals, as is seen in wild type.
Expression of ftΔD.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. However the wings of the rescued flies are 29% larger than normal and the distance between the anterior and posterior crossveins in 73% of wild type. Only a negligible wing hair planar cell polarity phenotype is seen. The abdominal hair polarity phenotype is fully rescued. The intercellular ridges continue to run from anterior to posterior as in the ft8/ftG-rv mutant posterior wings, rather than proximally to distal, as is seen in wild type.
Expression of ftΔE.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. However the wings of the rescued flies are 8% larger than normal and the distance between the anterior and posterior crossveins in 87% of wild type. Only a negligible wing hair planar cell polarity phenotype is seen. The abdominal hair polarity phenotype is fully rescued. The intercellular ridges that run from anterior to posterior in the ft8/ftG-rv mutant posterior wings run proximally to distal in rescue animals, as is seen in wild type.
Expression of ftΔF.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. However the wings of the rescued flies are 4% larger than normal and shorter and wider than normal wings. The distance between the anterior and posterior crossveins is also reduced. Wing hair planar cell polarity phenotypes are still present, with the most proximal part of the wing, the costa, the most severely affected. A subtle phenotype is observed in the abdomen. The intercellular ridges continue to run from anterior to posterior as in the ft8/ftG-rv mutant posterior wings, rather than proximally to distal, as is seen in wild type.
Expression of ftΔEGF.T:SV5\V5 fails to rescue the lethality associated with ft8/ftG-rv.
Expression of one copy of ftΔF.T:SV5\V5 partially suppresses the wing overgrowth phenotype seen in ft8/ftG-rv mutant flies expressing one copy of ftΔD.T:SV5\V5.
Expression of one copy of ftΔD.T:SV5\V5 partially suppresses the wing crossvein spacing and hair polarity phenotypes seen in ft8/ftG-rv mutant flies expressing one copy of ftΔF.T:SV5\V5.
Expression of ftΔICD.T:SV5\V5 fails to rescue the lethality associated with ft8/ftG-rv and the wing discs these flies are overgrown compared to controls.
Expression of ftP32.T:SV5\V5 rescues the lethality and wing overgrowth phenotype associated with ft8/ftG-rv.
Expression of ftmI.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. However the wings of the rescued flies are 18% larger than normal. Wing hair polarity defects are partially rescued and the abdominal hair defects are fully rescued.
Expression of ftmV.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. However the wings of the rescued flies are larger than normal.
Expression of ftmIV.T:SV5\V5 rescues the lethality associated with ft8/ftG-rv. The abdominal and wing hair polarity phenotypes are fully rescued.
for both ft alleles in GA22:
Expression of ftScer\UAS.cMa under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals substantially improves hair planar cell polarity defects in the abdomen.
Expression of ftΔECD.Scer\UAS.T:Ivir\HA1 or ftΔECD2.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals rescues disc overgrowth, allows eclosion and substantially improves hair planar cell polarity defects in the wing and abdomen.
Expression of ftICD.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals does not rescue disc overgrowth or eclosion and does not detectably improve hair planar cell polarity defects in the abdomen.
Expression of ftΔECDΔN-1.Scer\UAS.T:Ivir\HA1 or ftΔECDΔN-2.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals rescues eclosion and very weakly improves hair planar cell polarity defects in the abdomen.
Expression of ftΔECDΔN-4.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals rescues eclosion but does not detectably improve hair planar cell polarity defects in the abdomen.
Expression of ftΔECDΔN-5.Scer\UAS.T:Ivir\HA1, ftΔECDΔN-6.Scer\UAS.T:Ivir\HA1 or ftΔECDΔICD.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals does not rescue eclosion and does not detectably improve hair planar cell polarity defects in the abdomen.
Expression of ftΔECDΔ9-C.Scer\UAS.T:Ivir\HA1, ftΔECDΔ8-C.Scer\UAS.T:Ivir\HA1, ftΔECDΔ7-C.Scer\UAS.T:Ivir\HA1, ftΔECDΔ6-C.Scer\UAS.T:Ivir\HA1, ftΔECDΔ1-3.Scer\UAS.T:Ivir\HA1, ftΔECDΔ3-4.Scer\UAS.T:Ivir\HA1, ftΔ9-C.Scer\UAS.T:Ivir\HA1 or ftΔ7-C.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals rescues eclosion and strongly improves hair planar cell polarity defects in the abdomen.
Expression of ftΔECDΔ5-C.Scer\UAS, ftΔECDΔ4-C.Scer\UAS.T:Ivir\HA1, ftΔECDΔ2-C.Scer\UAS.T:Ivir\HA1, ftΔECDΔ1-C.Scer\UAS.T:Ivir\HA1, ftΔECDΔ5-6.Scer\UAS.T:Ivir\HA1, ftΔECDΔ3-5.Scer\UAS.T:Ivir\HA1, ftΔECDΔ1-5.Scer\UAS.T:Ivir\HA1, ftΔ6-C.Scer\UAS.T:Ivir\HA1 or ftΔ5-C.Scer\UAS under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals does not rescue eclosion but strongly improves hair planar cell polarity defects in the abdomen of pharate adults.
Expression of ftΔECDΔN-1.Scer\UAS.T:Ivir\HA1 or ftΔECDΔN-2.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals does not rescue hair planar cell polarity defects in the wing.
Expression of ftΔN-1.Scer\UAS.T:Ivir\HA1 or ftΔN-2.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals rescues eclosion and weakly improves hair planar cell polarity defects in the abdomen.
Expression of ftΔN-4.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals weakly rescues eclosion and very weakly improves hair planar cell polarity defects in the abdomen.
Expression of ftΔN-5.Scer\UAS.T:Ivir\HA1, ftΔN-6.Scer\UAS.T:Ivir\HA1 or ftΔICD.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals does not rescue eclosion but very weakly improves hair planar cell polarity defects in the abdomen of pharate adults.
Expression of ftΔ8-C.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in ftG-rv/ft8 animals weakly rescues eclosion and strongly improves hair planar cell polarity defects in the abdomen.
Expression of ftScer\UAS.cMa or ftΔECD.Scer\UAS in the posterior wing pouch, driven by Scer\GAL4en-e16E, rescues the overgrowth phenotype of ftG-rv/ft8 mutants in this region, but does not rescue overgrowth in the anterior.
Expression of ftΔECD.Scer\UAS, under the control of either Scer\GAL4Act5C.PI or Scer\GAL4da.G32, rescues the lethality of ftG-rv/ft8 mutants and is effective in rescuing the wing disc overgrowth phenotype.
Expression of ftΔECD.Scer\UAS under the control of Scer\GAL4Act5C.PI in ftG-rv/ft8 mutants partially rescues the abdominal planar cell polarity defect.
Expression of ftScer\UAS.cMa under the control of Scer\GAL4Act5C.PI rescues the lethality and wing disc overgrowth phenotype of ftG-rv/ft8 mutants and partially rescues the abdominal planar cell polarity defect.
ftScer\UAS.cMa; Scer\GAL4da.G32 rescues the pupal lethality of ftG-rv/ft8 and substantially rescues planar cell polarity and tarsal segment defects in these animals.
Bridges, 1930.
Revertant.