|Feature type||allele||Associated gene||Dmel\fu|
|Also Known As||fuJB3|
|Map ( GBrowse )|
|Allele class||hypomorphic allele - genetic evidence|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Defined by the absence of residues 29 to 37 in the N-terminal catalytic domain.
40bp deletion of genomic sequences 1002-1041, deletion of the 3' part of the first exon and 5' part of the first intron.
Mutation in the kinase domain. Class I fu allele, affects the catalytic domain but does not change the open reading frame.
Small deletion within putative kinase domain.
|Phenotype Manifest In|
4 day old fu homozygous females, only 11% of egg chambers are tumorous, increasing to 35% at 10 days. This phenotype is stronger in fu/fu females, in which 83% of egg chambers are tumorous ay 4 days, decreasing to 71% at 10 days. This is stronger than the phenotype in fu/Df(1)fu-Z4 females, where around 50% of egg chambers are tumorous at 4 or 10 days. Tumorous follicles in these ovarioles consist of tens to hundreds of germ-line cells with small non-polyploid nuclei enveloped by a regular follicular epithelium. Marker expression studies and analysis of spectrosome morphology in tumorous cysts from fu/Df(1)fu-Z4 animals suggest that these tumorous germ-line cells remain very immature and do not progress beyond the early cystoblast stage and may include germline stem cells (GSCs). However, the size of germline stem cell niches appears to be unnaffected - suggesting that these phenotypes are due to inceases in cystoblast-like cells outside of the niche. This increase is not due to increased cell division of germline stem cells in the niche - as assayed by scoring the percentage of GSCs in the niche undergoing mitosis in fu/fu fu/fu and fu/Df(1)fu-Z4 adult females. fu homozygous ovaries also contain egg chambers with increased numbers of germ cells that, based on marker expression and fusome morphology, have developed past the cystolast stage but are still more immature than normally found in female germline cysts outside of the germarium. There is some evidence of oocytes and nurse cell determination, but nurse cell and oocyte differentation appear to be blocked.
fu52/+ heterozygotes have a partial fusion of wing veins L3 and L4.
Homozygous females show a delay in the polar cell differentiation program. Restriction in the final number of polar cells is achieved by stage 5 in these females.
Mutant ovaries contain some egg chambers with more or less than the normal number of 16 germline cells. When an egg chamber contains less than 15 nurse cells, the complementary nurse cells are sometimes found in an adjacent egg chamber. In one case, an egg chamber containing two oocytes has been observed, indicating a multicyst egg chamber. In some cases the follicular epithelia of two adjacent egg chambers are apposed with no interfollicular stalk between them. The same ovariole can contain both normal and abnormal egg chambers. Degenerating egg chambers are seen. More dividing cells are seen in germarial regions 2 and 3 than in wild type. Mutant germaria show impaired encapsulation of the germline cysts by prefollicular cells, which results in some multicyst chambers. Interfollicular stalk cells do appear to be specified in the mutant germaria, but they are unable to fulfill their role in budding off of individual egg chambers. When stalk-like structures are seen between egg chambers they have an abnormal morphology, consisting of aggregates of round cells arranged in a ball shape (instead of the wild-type linear arrangement of 5-7 oval shaped cells). There is a delay in polar follicle cell specification. Clones induced in the somatic stem cells of the ovarioles can result in long, disorganised stalks within the ovariole, from which egg chambers show an off centre attachment. In most cases, these stalks are composed of fu+ cells, while the mutant cells are found as part of the follicular epithelium of adjacent chambers.
Mutants show fusion of wing veins L3 and L4 both proximally and distally, with intervein tissue remaining between the two veins in the medial part of the wing.
Weak wing phenotype (LV3 and LV4 fused proximally), reduced viability, strong fecundity phenotype.
Weak wing phenotype. Weak viability phenotype.
|Phenotype Manifest In|
fu52 has ovariole phenotype, suppressible | partially by ciCe-2.Scer\UAS.T:Ivir\HA1/Scer\GAL4Act5C.PP
Hsap\fuScer\UAS.Δ77-98; Scer\GAL4ptc-559.1 enhances the partial fusion of wing veins L3 and L4 seen in fu52 flies.
|Complementation & Rescue Data|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 11 )|