FlyBase Allele Report: Dmel\fy[2]

General Information

Symbol

Dmel\fy²

Species

D. melanogaster

Name

FlyBase ID

FBal0004916

Feature type

allele

Associated gene

Dmel\fy

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Collier and Gubb, 1997)

Mutagen

ethyl methanesulfonate

(Collier and Gubb, 1997, Grell, 1969)

Progenitor genotype

Cytology

Description

Contains a nonsense mutation which is predicted to result in a protein lacking the C-terminal 220 amino acids of fy.

(Collier and Gubb, 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,402,433..8,402,433 [-]

Nucleotide change:

G8402433A

Reported nucleotide change:

G?A

Amino acid change:

W197term | fy-PA; W197term | fy-PB

Reported amino acid change:

??term

(Collier and Gubb, 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity

(Lee and Adler, 2002, Grell, 1969)

abnormal planar polarity (with fy⁶)

(Lee and Adler, 2002)

abnormal planar polarity | adult stage

(Wang et al., 2017)

viable

(Collier and Gubb, 1997)

visible

(Grell, 1969)

visible | adult stage

(Wang et al., 2017)

visible | recessive

(Collier and Gubb, 1997)

Phenotype Manifest In

abdominal sternite bristle

(Wang et al., 2017)

macrochaeta

(Grell, 1969)

microchaeta & adult thorax

(Grell, 1969)

wing & trichome

(Collier and Gubb, 1997)

wing cell

(Wong and Adler, 1993)

wing hair

(Wong and Adler, 1993)

wing hair (with fy⁶)

(Lee and Adler, 2002)

wing hair & 1st posterior cell

(Lee and Adler, 2002)

wing hair & 3rd posterior cell

(Lee and Adler, 2002)

wing margin bristle

(Grell, 1969)

Detailed Description

Statement

Reference

fy² homozygous adults show hair polarity defects in abdominal bristles.

(Wang et al., 2017)

Wing hairs in the C region and E region of the wing point in a posterior direction. fy⁶/fy² wings show a weak polarity phenotype with occasional multiple wing hairs.

(Lee and Adler, 2002)

Hemizygous flies show an altered pattern of hair orientation on the wings. The phenotype is significantly stronger at 18^oC than at 29^oC. Hair polarity of homozygous clones in the wing resembles that seen in the wings of homozygous mutant flies, except near the borders of the clone, where the polarity is intermediate between mutant and wild-type. fy²/fy⁵ and fy²/fy⁶ flies have a stronger phenotype than fy⁵ and fy⁶ hemizygotes respectively, suggesting that fy² shows some antimorphic character in combination with hypomorphic alleles.

(Collier and Gubb, 1997)

Many wing cells of homozygous flies form two or three hairs. Homozygous flies have disruptions in the pattern of wing hair polarity. The prehair initiation site is along the cell periphery, but not restricted to the distal vertex in pupal wing cells. Double mutants of fy² with fz¹, dsh¹ or pk¹ resemble fy² single mutants. Double mutants of fy² with mwh¹ resemble mwh¹ single mutants.

(Wong and Adler, 1993)

Rank: RK1 Macrochaetae are somewhat disarranged. Microchaetae on the thorax point towards the midline. Microchaetae on the wing margin stand out and are slightly irregular. The phenotype resembles in and fz mutants.

(Grell, 1969)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fy²/fy⁶ has abnormal planar polarity phenotype, enhanceable by Scer\GAL4^bi-md653/stan^UAS.cUa

(Lee and Adler, 2002)

NOT Enhanced by

Statement

Reference

fy² has visible | adult stage phenotype, non-enhanceable by fz¹/fz¹

(Wang et al., 2017)

fy² has abnormal planar polarity | adult stage phenotype, non-enhanceable by fz¹/fz¹

(Wang et al., 2017)

fy² has visible | adult stage phenotype, non-enhanceable by ds^HMS00759/Scer\GAL4^ptc.PU

(Wang et al., 2017)

fy² has abnormal planar polarity | adult stage phenotype, non-enhanceable by ds^HMS00759/Scer\GAL4^ptc.PU

(Wang et al., 2017)

fy² has visible | adult stage phenotype, non-enhanceable by in^IH56/in^IH56

(Wang et al., 2017)

fy² has abnormal planar polarity | adult stage phenotype, non-enhanceable by in^IH56/in^IH56

(Wang et al., 2017)

fy²/fy⁶ has abnormal planar polarity phenotype, non-enhanceable by Scer\GAL4^ptc-559.1/pk^UAS.cGa

(Lee and Adler, 2002)

Enhancer of

Statement

Reference

fy[+]/fy² is an enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

NOT Enhancer of

Statement

Reference

fy²/fy² is a non-enhancer of visible | adult stage phenotype of in^IH56

(Wang et al., 2017)

fy²/fy² is a non-enhancer of abnormal planar polarity | adult stage phenotype of in^IH56

(Wang et al., 2017)

Suppressor of

Statement

Reference

fy² is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^Act5C.PI, pk^sple.UAS

(Lee and Adler, 2002)

fy² is a suppressor of abnormal planar polarity | heat sensitive phenotype of fz^hs.PP

(Lee and Adler, 2002)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, fy² has abnormal planar polarity phenotype

(Lee and Adler, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

fy²/fy⁶ has wing hair phenotype, enhanceable by Scer\GAL4^bi-md653/stan^UAS.cUa

(Lee and Adler, 2002)

fy²/fy⁶ has wing hair phenotype, enhanceable by fz²³/fz³⁰

(Lee and Adler, 2002)

fy² has wing hair & 1st posterior cell phenotype, enhanceable by mwh[+]/mwh¹

(Lee and Adler, 2002)

NOT Enhanced by

Statement

Reference

fy² has abdominal sternite bristle phenotype, non-enhanceable by fz¹/fz¹

(Wang et al., 2017)

fy² has abdominal sternite bristle phenotype, non-enhanceable by ds^HMS00759/Scer\GAL4^ptc.PU

(Wang et al., 2017)

fy² has abdominal sternite bristle phenotype, non-enhanceable by in^IH56/in^IH56

(Wang et al., 2017)

fy²/fy⁶ has wing hair phenotype, non-enhanceable by Scer\GAL4^ptc-559.1/pk^UAS.cGa

(Lee and Adler, 2002)

Enhancer of

Statement

Reference

fy[+]/fy² is an enhancer of wing hair | increased number phenotype of Scer\GAL4^en.PU, cmb^{UAS.RA.Tag:FLAG}

(Fagan et al., 2014)

fy[+]/fy² is an enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

NOT Enhancer of

Statement

Reference

fy²/fy² is a non-enhancer of abdominal sternite bristle phenotype of in^IH56

(Wang et al., 2017)

Suppressor of

Statement

Reference

fy² is a suppressor of wing hair phenotype of Scer\GAL4^Act5C.PI, pk^sple.UAS

(Lee and Adler, 2002)

fy² is a suppressor of wing hair | heat sensitive phenotype of fz^hs.PP

(Lee and Adler, 2002)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, fy² has macrochaeta phenotype

(Lee and Adler, 2002)

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, fy² has wing hair & 1st posterior cell phenotype

(Lee and Adler, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

fy²;fz¹ double mutant adults as well as fy² mutants expressing ds^HMS00759 under the control of the Scer\GAL4^ptc.PU driver display hair polarity defects in abdominal bristles that are similarly severe as in fy² single mutants. fy²/in^IH56 double mutants show abdominal bristle polarity phenotype that is comparable to each of the single mutants.

(Wang et al., 2017)

Heterozygosity significantly enhances the multiple hair cell phenotype of Scer\GAL4^en.PU cmb^Scer\UAS.RA wings.

(Fagan et al., 2014)

Over-expression of stan by Scer\GAL4^bi-md653 induces an increase in the number of multiple wing hairs in fy⁶/fy² wings. The multiple hair cell phenotype of dsh^Scer\UAS.cAa expressed from Scer\GAL4^ptc-559.1 is additive with the multiple hair phenotypes of fy² and in^IH56.