Open Close
General Information
Symbol
Dmel\fz37
Species
D. melanogaster
Name
FlyBase ID
FBal0004953
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
fzKD4A, KD4a
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Imprecise excision of the P-element, resulting in the deletion of more than 50kb flanking genomic sequences.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
external sensory organ precursor cell I & centrosome (with fz30)
external sensory organ precursor cell I & spindle (with fz30)
macrochaeta & eye
mesothoracic tergum & sensory mother cell & spindle (with fz30)
spindle & external sensory organ precursor cell I (with fz30)
Detailed Description
Statement
Reference
fz37/fz30 show defects in the orientation of the mitotic spindle in dividing pI sensory precursor cells.
In fz30/fz37 pupae, the mitotic spindles of dividing sensory organ precursor cells in the developing notum are not aligned with the anterior-posterior axis, but instead are randomly oriented.
In fz37/fz30 mutant pupae, unlike wild-type, the pI cells exhibit almost no centrosome rotation before spindle formation. Complete rotation values for the spindle are similar to wild-type, however in 7% of mutant pI cells, the spindle rotates in one direction before rotating in the opposite direction, bidirectional movements are not seen in wild-type. The spindle does not line up properly with the pon crescent, leading to defective partitioning of pon. 5% of fz37/fz30 flies have at least one bristle with two shafts and two sockets on the notum.
Only 47.1 +/- 8.9 % of ommatidia in fz37/fz15 flies have the correct chiral shape.
The orientation of projections of R1-R6 axons onto the lamina is normal for those ommatidia that are correctly orientated, and rotated for those ommatidia that are rotated.
Dendritic development in homozygous mutants is indistinguishable from wild-type.
Mosaic ommatidia at the boundaries of homozygous clones can show incorrect chirality. In incorrect chiral ommatidia where the mosaic boundary separates the R3/R4 pair, the R4 cell is invariably mutant and the R3 cell is wild type. In these cases the presumptive R3 cell has developed as an R4 cell. The R3/R4 pair can also either both be mutant or both be wild-type in mosaic ommatidia with incorrect chirality. Chirally incorrect ommatidia that have wild-type R3 and R4 cells can occur at any position along the border of the clone, but are predominantly found at the polar side of the clone. Mosaic ommatidia at the boundaries of homozygous clones can show correct chirality. In those ommatidia with mosaicism between R3/R4, the R3 cell is almost always wild type and the R4 cell is almost always mutant. Mosaic ommatidia at the boundaries of homozygous clones are occasionally symmetrical, and in these cases both members of the R3/R4 pair are generally mutant. Expression of fzhs.sev in a fz37 background in either the presumptive R3 or R4 cell in mosaic ommatidia causes the cell expressing fzhs.sev to become an R3 cell and the other to become an R4 cell.
In fz3/fz37 transheterozygotes the socket, shaft and pIIb cells are always localised in the region of the cell in contact with pIIb. The socket cells always occupies an eccentric position.
Rough eye phenotype, facets have lost their normal hexagonal shape and exhibit disarranged bristles. The ommatidial polarity is disrupted due to randomised direction of ommatidial rotation, insufficient degree of rotation and incorrect asymmetry established between R3 and R4 cells.
Strong phenotype.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
fz37/fz30 has eo neuron phenotype, enhanceable by numb796
fz37/fz30 has thecogen cell phenotype, enhanceable by numb796
Suppressed by
Statement
Reference
Scer\GAL4ptc-559.1, fz37 has trichome & adult abdomen phenotype, suppressible by fz21, fz37
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
fz21, fz37 is a suppressor of trichome & adult abdomen phenotype of Scer\GAL4ptc-559.1, fz37
Additional Comments
Genetic Interactions
Statement
Reference
The zone of reversed polarity at the back and behind the anterior compartment of each abdominal segment of stanScer\UAS.cUa; Scer\GAL4ptc-559.1 adults is suppressed by fz37/fz21.
When ft8 homozygous mutant clones are made in the R3/R4 precursors of fz37/fz15 mutant flies, The mutant photoreceptor precursor eventually becomes an R3 photoreceptor 61% of the time (50% in wild-type).
The addition of numb796 to fz37/fz30, leads to pupae that have sense organs composed of four cells with two socket cells but devoid of either sheath or neuronal cells.
fz22-2-2.hs.2sev does not rescue the tissue polarity defect seen in fz37/fz15 eyes. fz::fz22-2-1.hs.2sev does not rescue the tissue polarity defect seen in fz37/fz15 eyes. fz::fz22-1-1.hs.2sev does not rescue the tissue polarity defect seen in fz37/fz15 eyes. fz::fz21-2-2.hs.2sev does not rescue the tissue polarity defect seen in fz37/fz15 eyes. fz::fz21-1-2.hs.2sev significantly rescues the tissue polarity defect seen in fz37/fz15 eyes, such that 93.3 +/- 5.0 % of ommatidia have the correct chiral shape. fz::fz21-2-1.hs.2sev significantly rescues the tissue polarity defect seen in fz37/fz15 eyes, such that 76.5 +/- 6.4 % of ommatidia have the correct chiral shape.
nmoP1, fz37 double mutant eyes display a large number of ommatidia that do not rotate at all compared to either nmoP1 or fz37 mutants alone.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
fz37 is partially rescued by fzsev.PT
fz37 is partially rescued by fzGMR.PZ
Comments
fzarm.T:Avic\GFP-EGFP rescues oriented cell division in the pupal notum of fz30/fz37 animals.
fzGMR.PZ can almost completely rescue the fz37 mutant eye phenotype.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Adler.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (21)