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General Information
Symbol
Dmel\fzy1
Species
D. melanogaster
Name
FlyBase ID
FBal0004955
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

mitotic cell cycle & epidermis & embryo | ventral

mitotic cell cycle & spindle & epidermis & embryo | ventral

Detailed Description
Statement
Reference

fzy5032/fzy1 mutant adults possess all appendages but have a dramatically reduced neuropil compared to controls. They exhibit poor motor function.

fzy5032/fzy1 larval brains show progressive neuroblast loss.

fzy5032/fzy1 mutant exhibit morphological and molecular marker changes consistent with aberrant onset of necrosis: in contrast to wild type, neuroblasts in fzy5032/fzy1 mutant brains often do not have identifiable nuclei, and their cytoplasm appears largely devoid of organelles and vacuoles. In these neuroblasts mitochondria are swollen and ruptured and remnants of the endoplasmic reticulum clump with remaining mitochondria into electron dense aggregates. Mitochondrial aggregates are seen in 25% of remaining fzy5032/fzy1 mutant neuroblasts. Elevated levels of reactive oxygen species and calcium ions are seen in fzy5032/fzy1 mutant neuroblasts compared to controls. Almost 40% of fzy5032/fzy1 mutant neuroblasts contain ubiquitin-conjugated protein aggregates, and these are virtually non-existent in controls.

fzyAC-10/fzy1 larval brains show progressive neuroblast loss.

Homozygous germline stem cell clones are lost more quickly from ovaries compared to control clones.

More than 80% of cells in the ventral epidermis (VE) are arrested at metaphase in late stage 11 mutant embryos (compared to less than 5% of VE cells in wild-type embryos) and 62% of the centriole pairs at the spindle poles are engaged. More than 95% of the cells in the VE are in mitosis in early and mid-stage 12 mutant embryos (there are virtually no cells in mitosis in the VE at these stages in wild-type embryos) and all centriole pairs are disengaged.

The maternal fzy contribution is stable enough to allow completion of mitotic proliferation in the dorsal epidermis, but not in the ventral epidermis. fzy1 embryos carrying the temperature-sensitive cdc2A171T in P{Dmcdc2A171T} at 18oC exhibit mitotic arrest at stage 11 in the ventral region, as expected. Temperature shift to the restrictive temperature causes a drastic reduction in the number of metaphase arrested cells.

Ventral epidermis absent, CNS degenerate, PNS absent.

embryonic lethal ventral outside and central nervous system degenerate.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
NOT Suppressor of
Statement
Reference

fzy[+]/fzy1 is a non-suppressor of increased cell death phenotype of shtd1

Phenotype Manifest In
NOT suppressed by
Statement
Reference

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Atg125/Atg13

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Atg7d77

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Dronc51

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Dark82

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by DreddB118

NOT Enhancer of
Statement
Reference

fzy1 is a non-enhancer of phenotype of Mybunspecified

NOT Suppressor of
Statement
Reference

fzy[+]/fzy1 is a non-suppressor of eye phenotype of shtd1

fzy[+]/fzy1 is a non-suppressor of ommatidium phenotype of shtd1

fzy1 is a non-suppressor of phenotype of Mybunspecified

Additional Comments
Genetic Interactions
Statement
Reference

Atg7d77 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

DreddB118 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Ark82 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Nc51 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Atg125/Atg13 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

The addition of fzy1 has no effect on Mybunspecified viability.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

Expression of fzytDa rescues the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Expression of fzytDa rescues the neuroblast loss seen in fzyAC-10/fzy1 mutant larval brains.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
Comments

Phenotypic data included in FBrf0051973.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (21)