FlyBase Allele Report: Dmel\fzy[1]

General Information

Symbol

Dmel\fzy¹

Species

D. melanogaster

Name

FlyBase ID

FBal0004955

Feature type

allele

Associated gene

Dmel\fzy

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Philp and Glover, 1997, Sigrist et al., 1995, Dawson et al., 1993)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

female germline stem cell | germline clone

(Chen et al., 2009)

mitotic cell cycle & epidermis & embryo | ventral

(Vidwans et al., 1999)

mitotic cell cycle & spindle & epidermis & embryo | ventral

(Vidwans et al., 1999)

neuroblast | larval stage (with fzy⁵⁰³²)

(Kuang et al., 2014)

neuroblast | larval stage (with fzy^AC-10)

(Kuang et al., 2014)

neuropil | adult stage (with fzy⁵⁰³²)

(Kuang et al., 2014)

Detailed Description

Statement

Reference

fzy⁵⁰³²/fzy¹ mutant adults possess all appendages but have a dramatically reduced neuropil compared to controls. They exhibit poor motor function.

fzy⁵⁰³²/fzy¹ larval brains show progressive neuroblast loss.

fzy⁵⁰³²/fzy¹ mutant exhibit morphological and molecular marker changes consistent with aberrant onset of necrosis: in contrast to wild type, neuroblasts in fzy⁵⁰³²/fzy¹ mutant brains often do not have identifiable nuclei, and their cytoplasm appears largely devoid of organelles and vacuoles. In these neuroblasts mitochondria are swollen and ruptured and remnants of the endoplasmic reticulum clump with remaining mitochondria into electron dense aggregates. Mitochondrial aggregates are seen in 25% of remaining fzy⁵⁰³²/fzy¹ mutant neuroblasts. Elevated levels of reactive oxygen species and calcium ions are seen in fzy⁵⁰³²/fzy¹ mutant neuroblasts compared to controls. Almost 40% of fzy⁵⁰³²/fzy¹ mutant neuroblasts contain ubiquitin-conjugated protein aggregates, and these are virtually non-existent in controls.

fzy^AC-10/fzy¹ larval brains show progressive neuroblast loss.

(Kuang et al., 2014)

Homozygous germline stem cell clones are lost more quickly from ovaries compared to control clones.

(Chen et al., 2009)

More than 80% of cells in the ventral epidermis (VE) are arrested at metaphase in late stage 11 mutant embryos (compared to less than 5% of VE cells in wild-type embryos) and 62% of the centriole pairs at the spindle poles are engaged. More than 95% of the cells in the VE are in mitosis in early and mid-stage 12 mutant embryos (there are virtually no cells in mitosis in the VE at these stages in wild-type embryos) and all centriole pairs are disengaged.

(Vidwans et al., 1999)

The maternal fzy contribution is stable enough to allow completion of mitotic proliferation in the dorsal epidermis, but not in the ventral epidermis. fzy¹ embryos carrying the temperature-sensitive cdc2^A171T in P{Dmcdc2^A171T} at 18^oC exhibit mitotic arrest at stage 11 in the ventral region, as expected. Temperature shift to the restrictive temperature causes a drastic reduction in the number of metaphase arrested cells.

(Sigrist et al., 1995)

Ventral epidermis absent, CNS degenerate, PNS absent.

(Dawson et al., 1993)

embryonic lethal ventral outside and central nervous system degenerate.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

fzy¹/fzy⁵⁰³² has decreased cell number | larval stage phenotype, non-suppressible by Dredd^B118

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has decreased cell number | larval stage phenotype, non-suppressible by Atg7^d77

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Dronc⁵¹

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has decreased cell number | larval stage phenotype, non-suppressible by Dronc⁵¹

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Dark⁸²

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has decreased cell number | larval stage phenotype, non-suppressible by Dark⁸²

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Dredd^B118

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Atg1²⁵/Atg1³

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Atg7^d77

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has decreased cell number | larval stage phenotype, non-suppressible by Atg1²⁵/Atg1³

(Kuang et al., 2014)

NOT Suppressor of

Statement

Reference

fzy[+]/fzy¹ is a non-suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

fzy¹/fzy⁵⁰³² has neuroblast | larval stage phenotype, non-suppressible by Dronc⁵¹

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has neuroblast | larval stage phenotype, non-suppressible by Dark⁸²

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has neuroblast | larval stage phenotype, non-suppressible by Dredd^B118

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has neuroblast | larval stage phenotype, non-suppressible by Atg1²⁵/Atg1³

(Kuang et al., 2014)

fzy¹/fzy⁵⁰³² has neuroblast | larval stage phenotype, non-suppressible by Atg7^d77

(Kuang et al., 2014)

NOT Enhancer of

Statement

Reference

fzy¹ is a non-enhancer of phenotype of Myb^unspecified

(Fung et al., 2003)

NOT Suppressor of

Statement

Reference

fzy[+]/fzy¹ is a non-suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

fzy[+]/fzy¹ is a non-suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

fzy¹ is a non-suppressor of phenotype of Myb^unspecified

(Fung et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Atg7^d77 does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

Dredd^B118 does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

Ark⁸² does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

Nc⁵¹ does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

Atg1²⁵/Atg1³ does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

(Kuang et al., 2014)

The addition of fzy¹ has no effect on Myb^unspecified viability.

(Fung et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

fzy¹/fzy⁵⁰³² is rescued by fzy^tDa

(Kuang et al., 2014)

Comments

Expression of fzy^tDa rescues the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

Expression of fzy^tDa rescues the neuroblast loss seen in fzy^AC-10/fzy¹ mutant larval brains.

(Kuang et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

2492

fzy¹ cn¹ bw¹ speck¹/CyO

26661

Su(H)^IB115 fzy¹ cn¹ bw¹ speck¹/CyO

Kyoto

107364

fzy¹ cn¹ bw¹ speck¹/CyO

Notes on Origin

Discoverer

Comments

Phenotypic data included in FBrf0051973.

(Roote, 1995.5.4)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

fizzy1

(Vidwans et al., 1999)

fizzy1¹

(Fung et al., 2003)

fzy¹

(Das et al., 2016, Sarov et al., 2016, Kuang et al., 2014, Chen et al., 2009, Scaria et al., 2008, Slack et al., 2007, Tanaka-Matakatsu et al., 2007, Siegrist and Doe, 2005)

fzy^IB115

(Dawson et al., 1993)

fzy^IB

(Jack and Myette, 1999, Tearle and Nusslein-Volhard, 1987)

fzy^ID115

(Ashburner et al., 1990)

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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