we(g), g53d double mutant flies have at least a 50% reduction of brown (ommochrome) pigments deposited in the eye compared to g53d single mutants. There is also a reduction in red (pteridine) pigment to a lesser extent. All of the following w alleles when heterozygous with we(g) enhance the eye colour defective phenotype of g53d mutants, leading to a greater reduction of red pigment within the eye: w1118, w1, wa, wbf, wBwx, wco and wsat. In contrast to g53d homozygotes, gP/g53d transheterozygotes do interact with we(g), as gP/g53d, we(g) and gP/g53d, we(g)/+ flies have a reduced amount of red pigment in the eye compared to gP, we(g) double mutant flies. The wHSBJ transgene is able to increase the amount of red pigment in the eyes of g53d mutant flies. The g53d and following w alleles act additively to reduce eye pigmentation in double mutant male flies: wa, wa3, wbf, wch, wco, we, wi and wt. g53d, za double mutants have a reduced amount of red pigment in the eye comparted to g53d single mutants in both male and female flies. Likewise, g53d, z1 double mutant flies show decreased red eye pigment than g53d mutants, although this reduction is greater in female flies than in male flies. Male E(z)1/+, g53d, z1 triple mutants have less red pigment in the eye than g53d, z1 double mutants. Likewise, female E(z)1/+, g53d/+, z1/+ triple mutants have a reduction in red eye pigment compared to g53d/+, z1/+ double mutants.
Hexter, April 1953.