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General Information
Symbol
Dmel\gl3
Species
D. melanogaster
Name
FlyBase ID
FBal0005042
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
insertion site
Linked to:
XhoI-XhoI restriction fragment
Comment:

An insertion of about 2.5kb is within a XhoI fragment; position of restriction fragment on reference sequence inferred by FlyBase curator.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of about 2.5kb of unknown DNA (maybe a transposable element).

Insertion of about 2.5kb of unknown DNA into the gl locus.

Carried on aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In gl3 mutants, cellular structure of the retina is disorganized, although photoreceptor neurons survive metamorphosis and are still present in the adult retina, in contrast to previous reports.

gl3/gl3 and gl3/gl60j mutant adults possess fewer and fused ommatidia, and boundaries between ommatidia are difficult to distinguish, as compared to wild type.

gl3/gl3 mutant adults possess few differentiated photoreceptors, but rhabdomeres are morphologically disrupted, smaller and malformed, as compared to wild type. gl3/gl3 mutant third instar larvae display disrupted photoreceptor cluster patterning in the eye imaginal disc. At 48 h APF, gl3/gl3 mutants possess all expected cell types, photoreceptors, cone and pigment cells, as compared to wild type, however no ommatidia contains the correct number of each retinal cell type, there is complete disruption of organization of retinal cells into distinct identifiable ommatidia, and cone and primary pigment cells are either too few or too many in number.

At 96 h APF, gl3/gl60j mutants display increased cell death of photoreceptors in the retina.

In contrast to wild-type, gl3 mutants fail to exhibit any light-dependent temperature preference.

Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. Nonlinear mechanical amplification is significantly reduced compared to wild type.

Intermediate eye phenotype.

Displays locomotor activity rhythm with an approximately 24h period.

Weak allele. The eye is disorganised. Corneal hairs and red pigment granules are only present in the centre of the eye. The ocellar lenses are normal in size, but lack the bulge of the wild-type, and have small protuberances. There are no ocellar retinula cells. The lenses of the compound eye and ocelli are roughened with "corneal nipples".

Photoreceptor cells degenerate and die in the mid-pupal stage at the time when they would normally be producing rhabdomeres (FBrf0074259).

Adult eye is rough, reduced and has a glassy texture. Irregular ommatidia pattern, no rhabdomeres and masses of pigment granules.

Malpighian tubule colour: wild-type.

Weak allele. Eyes small and elliptical with surface less rough than gl1 or gl2. Eye colour: homogeneous scarlet. Ocellus colour: colourless. RK1.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference

gl3 has visible | adult stage phenotype, non-suppressible by Pph13hs.PL

NOT Enhancer of
Statement
Reference

gl3/gl[+] is a non-enhancer of visible | heat sensitive phenotype of peb1

Suppressor of
Statement
Reference

gl3/gl[+] is a suppressor of visible phenotype of upd1GMR.PB

gl3/gl[+] is a suppressor of size defective | adult stage phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

gl3/gl[+] is a non-suppressor of visible | heat sensitive phenotype of peb1

Phenotype Manifest In
Enhanced by
Statement
Reference

gl3 has phenotype, enhanceable by E(gl)3BSCG1

gl3 has phenotype, enhanceable by E(gl)3CSCG1

gl3 has phenotype, enhanceable by E(gl)3DSC1

gl3 has phenotype, enhanceable by E(gl)3DSCG1

gl3 has phenotype, enhanceable by Nl1N-ts1

gl3 has phenotype, enhanceable by Nspl-1

gl3 has phenotype, enhanceable by dogSC1

gl3 has phenotype, enhanceable by dogSC2

gl3 has phenotype, enhanceable by hSCG1

gl3 has phenotype, enhanceable by hhSC10

gl3 has phenotype, enhanceable by E(gl)2ASC1

gl3 has phenotype, enhanceable by hhSC11

gl3 has phenotype, enhanceable by hhSC12

gl3 has phenotype, enhanceable by hhSC13

gl3 has phenotype, enhanceable by hhSC14

gl3 has phenotype, enhanceable by hhSC15

gl3 has phenotype, enhanceable by hhSC16

gl3 has phenotype, enhanceable by hhSC17

gl3 has phenotype, enhanceable by hhSC18

gl3 has phenotype, enhanceable by hhSC19

gl3 has phenotype, enhanceable by hhSC1

gl3 has phenotype, enhanceable by E(gl)2BSC1

gl3 has phenotype, enhanceable by hhSC20

gl3 has phenotype, enhanceable by hhSC21

gl3 has phenotype, enhanceable by hhSC2

gl3 has phenotype, enhanceable by hhSC3

gl3 has phenotype, enhanceable by hhSC4

gl3 has phenotype, enhanceable by hhSC5

gl3 has phenotype, enhanceable by hhSC6

gl3 has phenotype, enhanceable by hhSC7

gl3 has phenotype, enhanceable by hhSC8

gl3 has phenotype, enhanceable by hhSC9

gl3 has phenotype, enhanceable by E(gl)2CSC1

gl3 has phenotype, enhanceable by hhSCG1

gl3 has phenotype, enhanceable by hhSCG2

gl3 has phenotype, enhanceable by hhSCG3

gl3 has phenotype, enhanceable by hhfse

gl3 has phenotype, enhanceable by hhts2

gl3 has phenotype, enhanceable by Df(3R)rn20

gl3 has phenotype, enhanceable by rnroe-1

gl3 has phenotype, enhanceable by rnroe-3

gl3 has phenotype, enhanceable by rnroe-4

gl3 has phenotype, enhanceable by rnroe-SC1

gl3 has phenotype, enhanceable by E(gl)2DSC1

gl3 has phenotype, enhanceable by E(gl)2ESC1

gl3 has phenotype, enhanceable by E(gl)2FSC1

gl3 has phenotype, enhanceable by E(gl)2HSC1

gl3 has phenotype, enhanceable by E(gl)3ASC1

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

gl3 has eye phenotype, non-suppressible by Pph13hs.PL

NOT Enhancer of
Statement
Reference

gl3/gl[+] is a non-enhancer of eye | heat sensitive phenotype of peb1

Suppressor of
Statement
Reference

gl3/gl[+] is a suppressor of eye phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

gl3/gl[+] is a non-suppressor of eye | heat sensitive phenotype of peb1

Additional Comments
Genetic Interactions
Statement
Reference

Pulsing Pph13hs.PL expression using heat shocks rescues photoreceptor differentiation in gl3/gl3 mutant retinas, as indicated by the presence of definable rhabdomeres at 96 h APF, but does not further disrupt or rescue overall eye morphology of gl3/gl3 mutants.

The enlarged eye phenotype seen in flies carrying one copy of upd1GMR.PB is suppressed by one copy of gl3.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Rescued by
Partially rescued by

gl3/gl60j is partially rescued by glGFP.FPTB

gl3 is partially rescued by glhs.PL

gl3 is partially rescued by glhs.PL

Comments

Periodic pulses after 24 h APF of glhs.PL using heat shocks neither rescues or induces additional defects with respect to eye morphology, but restores photoreceptor differentiation in gl3/gl3 mutant retinas, as indicated by the presence of rhabdomeres separated by a distinct inter-rhabdomeral space, although many of these ommatidia still do not contain the full complement of eight photoreceptors.

Expression of a single copy of glT:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP rescues the overall external morphology of the eye, but not the photoreceptor differentiation defects of adult gl3/gl60j mutants.

Normal light-dependent temperature preference and DN1p neurons are restored in gl3 mutants by gl+t9954.

Images (1)
Stocks (11)
Notes on Origin
Discoverer

Stern.

Comments
Comments

Weak gl allele.

In a screen for dominant suppressors of hh, 25 out of 84 mutations recovered were at hh.

No interaction with P{sev-svp1} or P{sev-svp2} exists.

Weak gl mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (27)