Allele Dmel\Gl^l1

General Information
Symbol	Dmel\Gl^l1	Species	D. melanogaster
Name		FlyBase ID	FBal0005066
Feature type	allele	Associated gene	Dmel\Gl
Also Known As	Gl^1-3

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Kankel and Lipshitz, 1981)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q932@. (Siller et al., 2005) Nucleotide substitution: C?T. (Siller et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| first instar larval stage (with Gl^Δ22) (Lloyd et al., 2012) lethal \| pupal stage (with Gl^G38S) (Lloyd et al., 2012) lethal \| recessive lethal \| recessive \| second instar larval stage (Siller et al., 2005) lethal \| second instar larval stage (with Df(3L)fz-GF3b) (Siller et al., 2005) mitotic cell cycle defective (with Df(3L)fz-GF3b) (Siller et al., 2005)
Phenotype Manifest In
	cuticle eye spindle (with Gl^Δ22) (Siller et al., 2005) spindle pole centrosome (with Gl^Δ22) (Siller et al., 2005)
Detailed Description
	Statement Reference Gl^Δ22/Gl^l1 neuroblasts show centrosome and spindle formation defects. Defects include metaphase neuroblasts with curved spindles, unfocused spindle poles, spindles with one or two unattached centrosomes, spindles in which both centrosomes are attached to the same half-spindle, or occasionally neuroblasts with three or four centrosomes forming bipolar or multipolar spindles. The mitotic index and metaphase:anaphase ratio are increased in Gl^l1/Df(3L)fz-GF3b neuroblasts compared to wild type. Tension between sister centromeres is reduced in Gl^l1 metaphase neuroblasts compared to wild type. (Siller et al., 2005) Gl^l1/Df(3L)Gl2 animals die as embryos or early first instar larvae. Cell lethal in the eye or cuticle.
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Gl[l1] shows little or no dominant interaction with S[1] in the eye. (Iyadurai et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Gl^G38S/Gl^l1 is rescued by Gl^CH322-82J07 (Lloyd et al., 2012) Gl^Δ22/Gl^l1 is rescued by Gl^CH322-82J07 (Lloyd et al., 2012)
Partially rescued by	Gl^G38S/Gl^l1 is partially rescued by Gl^Scer\UAS.cLa/Scer\GAL4^T80 (Lloyd et al., 2012) Gl^Δ22/Gl^l1 is partially rescued by Gl^Scer\UAS.cLa/Scer\GAL4^T80 (Lloyd et al., 2012)
Comments	Expression of Gl[Scer\UAS.cLa] under the control of Scer\GAL4[T80] rescues the lethality of Gl[l1]/Gl[Δ22] and Gl[l1]/Gl[G38S] animals, although the rescued animals are sterile. Expression of Gl[CH322-82J07] fully rescues Gl[l1]/Gl[Δ22] and Gl[l1]/Gl[G38S] animals (the rescued animals are viable and fertile). (Lloyd et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Gl^1-3 (Siller et al., 2005, Iyadurai et al., 2008, Lloyd et al., 2012) Gl^l1 l(3)Gl^1-3 (Kankel and Lipshitz, 1981)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Lloyd et al., 2012, Neuron 74(2): 344--360 The p150(Glued) CAP-Gly Domain Regulates Initiation of Retrograde Transport at Synaptic Termini. [FBrf0218160] Iyadurai et al., 2008, J. Cell Sci. 121(16): 2643--2651 Dynein and Star interact in EGFR signaling and ligand trafficking. [FBrf0205741] Siller et al., 2005, Mol. Biol. Cell 16(11): 5127--5140 Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control. [FBrf0191164] Garen et al., 1984, Genetics 107: 645--655 Mutations affecting functions of the Drosophila gene Glued. [FBrf0040970] Harte and Kankel, 1982, Genetics 101: 477--501 Genetic analysis of mutations at the Glued locus and interacting loci in Drosophila melanogaster. [FBrf0038057] Kankel and Lipshitz, 1981, Hotta, 1981: 216--238 Allelic variability at loci affecting the development and function of the visual system. [FBrf0084083]

Allele Dmel\Gll1

Allele Dmel\Gl^l1