FB2025_01 , released February 20, 2025
Allele: Dmel\haync2
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General Information
Symbol
Dmel\haync2
Species
D. melanogaster
Name
FlyBase ID
FBal0005384
Feature type
allele
Associated gene
Dmel\hay
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

antimorphic allele - genetic evidence

temperature conditional hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

antimorphic allele - genetic evidence

(Mounkes and Fuller, 1999)

temperature conditional hypomorphic allele - genetic evidence

(Merino et al., 2002)
Mutagen
ethyl methanesulfonate
(Fuller, 1986)
Progenitor genotype
Cytology
Description

Amino acid replacement: R652C. R652 lies in the conserved helicase domain VI. Nucleotide substitution: C?T.

(Mounkes and Fuller, 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:10,633,469..10,633,469 [+]
Nucleotide change:

C10633469T

Reported nucleotide change:

C?T

Amino acid change:

R649C | hay-PA; R649C | hay-PB

Reported amino acid change:

R652C

Comment:

The amino acid numbering differs between FBrf0108244 and the reference sequence. The positions and nature of the base substitutions in the mutants are derived from the sequence in Fig. 1 and from Table 1, and provide unambiguous assignments.

(Mounkes and Fuller, 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Cockayne syndrome
      CEA
      (Mounkes et al., 1992)
      model of  xeroderma pigmentosum
      CEA
      (Mounkes et al., 1992)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal meiotic cell cycle | male | recessive

      (Regan and Fuller, 1990, Regan and Fuller, 1988)

      female semi-fertile

      female sterile | recessive | heat sensitive

      (Regan and Fuller, 1988)

      increased cell death

      (Merino et al., 2002)

      lethal (with haync2rv1), with hayTTD.Hsp83

      (Merino et al., 2002)

      lethal (with haync2rv1), with hayXPCS.Hsp83

      (Merino et al., 2002)

      lethal | recessive | heat sensitive

      (Aguilar-Fuentes et al., 2008)

      male sterile

      (Mounkes et al., 1992)

      male sterile | recessive

      (Fuller, 1986)

      male sterile | recessive | heat sensitive

      (Regan and Fuller, 1988)

      partially lethal (with Df(3L)lxd6)

      (Merino et al., 2002)

      partially lethal (with haync2rv1)

      (Merino et al., 2002)

      partially lethal (with haync2rv2)

      (Merino et al., 2002)

      partially lethal (with haync2rv4)

      (Merino et al., 2002)

      partially lethal (with haync2rv7)

      (Merino et al., 2002)

      partially lethal (with haync2rv8)

      (Merino et al., 2002)

      partially lethal | recessive

      (Merino et al., 2002)

      partially lethal - majority die | recessive | heat sensitive

      radiation sensitive

      (Mounkes and Fuller, 1999)

      radiation sensitive | recessive

      (Mounkes et al., 1992)

      viable

      (Mounkes and Fuller, 1999, Fuller, 1986)

      visible, with hayXPCS.Hsp83

      (Merino et al., 2002)

      visible (with Df(3L)lxd6)

      (Merino et al., 2002)

      visible (with haync2rv1)

      (Merino et al., 2002)

      visible (with haync2rv2)

      (Merino et al., 2002)

      visible (with haync2rv4)

      (Merino et al., 2002)

      visible (with haync2rv7)

      (Merino et al., 2002)

      visible (with haync2rv8)

      (Merino et al., 2002)

      visible | recessive

      (Merino et al., 2002)
      Phenotype Manifest In

      adult abdomen & endocuticle

      (Merino et al., 2002)

      adult abdomen & endocuticle (with Df(3L)lxd6)

      (Merino et al., 2002)

      adult abdomen & endocuticle (with haync2rv1)

      (Merino et al., 2002)

      adult abdomen & endocuticle (with haync2rv2)

      (Merino et al., 2002)

      adult abdomen & endocuticle (with haync2rv4)

      (Merino et al., 2002)

      adult abdomen & endocuticle (with haync2rv7)

      (Merino et al., 2002)

      adult abdomen & endocuticle (with haync2rv8)

      (Merino et al., 2002)

      chaeta, with hayXPCS.Hsp83

      (Merino et al., 2002)

      imaginal disc

      (Merino et al., 2002)

      larval central nervous system

      (Merino et al., 2002)

      macrochaeta

      (Merino et al., 2002)

      macrochaeta (with Df(3L)lxd6)

      (Merino et al., 2002)

      macrochaeta (with haync2rv1)

      (Merino et al., 2002)

      macrochaeta (with haync2rv2)

      (Merino et al., 2002)

      macrochaeta (with haync2rv4)

      (Merino et al., 2002)

      macrochaeta (with haync2rv7)

      (Merino et al., 2002)

      macrochaeta (with haync2rv8)

      (Merino et al., 2002)

      meiotic cell cycle | male

      (Fuller, 1986)

      microchaeta

      (Merino et al., 2002)

      microchaeta (with Df(3L)lxd6)

      (Merino et al., 2002)

      microchaeta (with haync2rv1)

      (Merino et al., 2002)

      microchaeta (with haync2rv2)

      (Merino et al., 2002)

      microchaeta (with haync2rv4)

      (Merino et al., 2002)

      microchaeta (with haync2rv7)

      (Merino et al., 2002)

      microchaeta (with haync2rv8)

      (Merino et al., 2002)

      wing

      (Merino et al., 2002)

      wing, with hayXPCS.Hsp83

      (Merino et al., 2002)

      wing (with haync2rv1)

      (Merino et al., 2002)

      wing (with haync2rv2)

      (Merino et al., 2002)

      wing (with haync2rv7)

      (Merino et al., 2002)

      wing margin

      (Merino et al., 2002)

      wing margin (with haync2rv1)

      (Merino et al., 2002)

      wing margin (with haync2rv2)

      (Merino et al., 2002)

      wing margin (with haync2rv7)

      (Merino et al., 2002)
      Detailed Description
      Statement
      Reference

      haync2/+ mutants do not show any changes in the number of mitotic cells in the third instar larval wing discs compared to wild-type controls.

      (Lee et al., 2015)

      Homozygotes are lethal at 25[o]C.

      (Aguilar-Fuentes et al., 2008)

      Homozygotes show 46.3% viability. 14% of homozygous adults have deformed and fragile macrochaetae and microchaetae, 13% have a reduction of the endocuticular layer of the abdominal cuticle and 9% show loss of wing marginal regions and blistered wings. haync2/haync2rv1 animals show 47.9% viability. 19.9% of haync2/haync2rv1 adults have deformed and fragile macrochaetae and microchaetae, 21.5% have a reduction of the endocuticular layer of the abdominal cuticle and 14.3% show loss of wing marginal regions and blistered wings. haync2/haync2rv2 animals show 45.6% viability. 49.1% of haync2/haync2rv2 adults have deformed and fragile macrochaetae and microchaetae, 12.0% have a reduction of the endocuticular layer of the abdominal cuticle and 9.6% show loss of wing marginal regions and blistered wings. haync2/haync2rv4 animals show 12.4% viability. 47.9% of haync2/haync2rv4 adults have deformed and fragile macrochaetae and microchaetae, 16.6% have a reduction of the endocuticular layer of the abdominal cuticle and 0% show loss of wing marginal regions and blistered wings. haync2/haync2rv7 animals show 38.8% viability. 46.1% of haync2/haync2rv7 adults have deformed and fragile macrochaetae and microchaetae, 16.7% have a reduction of the endocuticular layer of the abdominal cuticle and 1.0% show loss of wing marginal regions and blistered wings. haync2/haync2rv8 animals show 49.7% viability. 11.1% of haync2/haync2rv8 adults have deformed and fragile macrochaetae and microchaetae, 15.3% have a reduction of the endocuticular layer of the abdominal cuticle and 0% show loss of wing marginal regions and blistered wings. haync2/Df(3L)lxd6 animals show 50.0% viability. 26.6% of haync2/Df(3L)lxd6 adults have deformed and fragile macrochaetae and microchaetae, 26.0% have a reduction of the endocuticular layer of the abdominal cuticle and 0% show loss of wing marginal regions and blistered wings. One copy of hayTTD.Hsp83 or hayXPCS.Hsp83 decreases the viability of haync2/haync2rv1 or haync2/haync2 flies dramatically. haync2/+ flies carrying one copy of hayXPCS.Hsp83 have fragile and thin bristles and the wings are almost amorphous. These flies show 70% viability. haync2/+ flies carrying one copy of hayTTD.Hsp83 show 92% viability. Homozygous flies show an increase in apoptosis in the larval central nervous system and imaginal discs compared to wild type. The number of apoptotic cells in homozygous discs after UV irradiation is increased compared to irradiated wild-type discs.

      (Merino et al., 2002)

      Mutants are UV sensitive. Heterozygotes for haync2 are fertile.

      (Mounkes and Fuller, 1999)

      Flies heterozygous for this allele and a deficiency for hay show increased sensitivity to ultraviolet irradiation, but not MMS.

      (Mounkes et al., 1992)

      Dominant enhancer of tubulin mutants. Double heterozygotes with wrl1 are sterile.

      (Green et al., 1990)

      Heterozygotes exhibit defects in the structure and function of the male meiotic spindle.

      (Regan and Fuller, 1990)

      Defects in the microtubule based functions of spermatogenesis: abnormalities in the distribution of nuclear material and mitochondria in post-meiotic spermatid cysts. Semilethal: reduced viability of homozygous flies at elevated temperatures. Thoracic bristle defect: postscutellar bristles stand out from the body at different angles instead of lying flat.

      (Regan and Fuller, 1988)

      Homozygous males are sterile and have defects in meiosis, flagellar elongation and nuclear shaping.

      (Fuller, 1986)

      Reduced female fertility. Homozygous viable at 25oC but semi-lethal at 28oC. Dominant enhancer of tubulin mutations.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      haync2 has lethal | recessive | heat sensitive phenotype, suppressible by Tfb5Hsp83.PAF

      (Aguilar-Fuentes et al., 2008)
      Suppressor of
      Statement
      Reference

      hay[+]/haync2 is a suppressor of increased body size | third instar larval stage phenotype of hfpEP3058

      (Lee et al., 2015)

      hay[+]/haync2 is a suppressor of increased occurrence of cell division | third instar larval stage phenotype of hfpEP3058

      (Lee et al., 2015)

      hay[+]/haync2 is a suppressor of visible phenotype of Scer\GAL4Bx-MS1096, p53GUS.PB

      (Merino et al., 2002)
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      haync2 is an enhancer of phenotype of βTub85D3

      (Fuller, 1986)
      Suppressor of
      Statement
      Reference

      hay[+]/haync2 is a suppressor of wing disc | third instar larval stage phenotype of hfpEP3058

      (Lee et al., 2015)

      hay[+]/haync2 is a suppressor of wing phenotype of Scer\GAL4Bx-MS1096, p53GUS.PB

      (Merino et al., 2002)
      Other
      Statement
      Reference

      Cdk7P140S, hay[+]/haync2 has cuticle phenotype

      (Merino et al., 2002)

      Cdk7P140S, hay[+]/haync2 has chaeta phenotype

      (Merino et al., 2002)

      Cdk7P140S, haync2 has cuticle phenotype

      (Merino et al., 2002)

      Cdk7P140S, haync2 has chaeta phenotype

      (Merino et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The large body size as well as the elevated proliferation rate (number of mitotic and S-phase cells) in the wing disc characteristic for the hfpEP3058/hfpEP3058 third instar larvae can be restored by combination with haync2 in heterozygous state.

      haync2/+;MycG0354/+ double heterozygotes do not show any changes in the number of mitotic cells in the third instar larval wing discs compared to wild-type controls.

      The number of mitotic cells in the wing disc of hfpEP3058/hfpEP3058;haync2/+ third instar larvae is not changed by combination with MycG0354 in heterozygous state.

      (Lee et al., 2015)

      Df(1)JB254 P{snf+,dhd+}/+ ; haync2/+ flies do not show shape deformities or size reduction in the wing, do not have brittle bristles and do not have cuticle defects. Flies carrying Cdk7P140S in a heterozygous Df(1)JB254 P{snf+,dhd+} background (the Df(1)JB254 chromosome is deficient for Cdk7, snf and dhd and snf and dhd function is provided by the P{snf+,dhd+} transgene) at the restrictive temperature that are also carrying one copy of haync2 show brittle bristle and cuticle defect phenotypes. The wing defects caused by expression of p53GUS.PB under the control of Scer\GAL4Bx-MS1096 are completely suppressed by haync2/+.

      (Merino et al., 2002)

      Flies transheterozygous for haync2 and βTub85Dn are male sterile.

      (Mounkes and Fuller, 1999)

      Fails to complement βTub85D3.

      (Fuller, 1986)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      haync2 is rescued by hay+t7.8

      (Mounkes et al., 1992)
      Comments

      Male sterility rescued by genomic DNA fragment carrying hay transcription unit.

      (Mounkes et al., 1992)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a mutation that fails to complement a class I allele at the βTub85D locus (βTub85D3) but does not map to the βTub85D locus.

      (Fuller, 1986)
      Comments
      Comments

      Fails to complement all class I B2t alleles, due to the common biochemical defect of reduced testis tubulin pools, and some of class II B2t alleles, due to the functional differenced between the mutant proteins.

      (Regan and Fuller, 1988)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Haync2
      (Lee et al., 2015)
      haync2
      (Cruz-Becerra et al., 2018, Aguilar-Fuentes et al., 2008)
      nc2
      (Fuller, 1986)
      Name Synonyms
      Secondary FlyBase IDs
        References (12)