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General Information
Symbol
Dmel\hh8
Species
D. melanogaster
Name
FlyBase ID
FBal0005469
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hh13C, hh13C29
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G23133049A

Amino acid change:

W232term | hh-PB

Reported amino acid change:

W232term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W232term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The eyes of hh8/hhbar3 flies are smaller than wild-type and are slightly rough, due to some disorganisation of ommatidial and inter-ommatidial structure.

Heterozygotes have wild-type eyes.

hhbar3/hh8 flies have a slight rough eye phenotype.

Homozygous hh8 mutants are zygotic lethal and exhibit strong cuticle phenotypes.

hh8 heterozygotes are not significantly different from wild-type.

Mutant embryos have a reduced number of visceral mesoderm parasegmental cells. The gastric caecum is virtually abolished in these embryos. When hh4/hh8 embryos are shifted from permissive to non-permissive temperatures for three hours during mid-stage 11 to stage 12, the majority have a short gastric caecum phenotype. While in about 10% one or few gastric caeca are lost and the size of those remaining is considerably reduced.

Most mutant embryos show a reduction (approximately 50%) in the number of cells in tracheal pit 3. 71% of embryos show a reduction of tracheal cell number in pits 2, 4, 8 or 9. A small number of tracheal cells fail to invaginate at stage 11 and remain on the ectoderm. In the tracheal cells that do invaginate, there is a distinct failure of migration of all tracheal branches at stage 12. This phenotype is penetrant in all mutant embryos. The dorsal and visceral branches are never formed, while the dorsal trunk cells migrate partially, especially in the posterior segments. A reduced number of cells is allocated to the lateral trunk anterior branch. The correct cell number is allocated to the lateral trunk posterior in most segments. Minimal tracheal cell migration is seen beyond the residual migration seen at stage 12 (in contrast to wild type where pronounced tracheal cell migration is seen between stages 12 to 14), with the majority of the tracheal cells remaining in the transverse connective.

Bolwig's organ neurons are missing in mutant embryos.

Denticle belts are made up of mostly type 5 denticles.

Homozygous embryos have severe head defects. The antennal sense organ is frequently duplicated.

Denticle belts are completely eliminated and the ventral surface of the cuticle is covered in a continuous lawn of denticles of similar size and random polarity.

The hh4/hh8 combination istemperature sensitive: loss of hh function 6hrs AEL leads to loss of 1o, 2o and 3o but not 4o fates. Loss of hh function 7hrs AEL leads to loss of 1o, 2o but not 3o and 4o fates. In double mutants with ptc9, 1o and 2o cell types are restored.

Most of the heart cells are missing in homozygous embryos. hh4/hh8 embryos raised at 29oC throughout development exhibit a severely reduced heart, at 18oC the number of heart cells is normal. Temperature shift experiments show heart formation is most sensitive between 3.5 and 4.5 hours of development. hh8, P{HS-wg} embryos kept at 25oC exhibit lack of heart formation, 30 minutes of heat shock between 3 and 4 hours of development significantly restores heart development. hh8, P{HShh.I} embryos that are heat shocked between 3 and 4 hours of development exhibit rescue (and hypertrophy) of heart precursors.

Naked cuticle and polarity of the embryo is lost.

Embryos exhibit a ventral cuticle phenotype.

hhbar3/hh6 produces an intermediate reduced eye phenotype.

Mutant embryos have exclusively row-5-type denticles arranged in a disorganized pattern of ventral midline whorls, with no naked cuticle.

Lethal when in homozygous or heterozygous combination with hhA, hhAC, hhAE or hhAM.

Severe disruptions in the neuron pattern occur during germ-band shortening, may be due to widespread cell death in the CNS.

40% length of wild type larvae and exhibit a ventral lawn of denticles associated with the loss of naked cuticle from the ventral surface. No obvious segmentation.

Strong phenotype. Mutant embryos show no obvious segmentation, and are 40% the length of wild type; denticles form a lawn arranged in a number of whorls on the ventral surface as a result of loss of naked cuticle.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

hhbar3/hh8 has visible phenotype, enhanceable by snamaPX1/mnm[+]

hhbar3/hh8 has visible phenotype, enhanceable by l(2)rQ313[+]/snamarQ313

hhbar3/hh8 has visible phenotype, enhanceable by snama1/mnm[+]

Suppressed by
Statement
Reference

hhbar3/hh8 has visible phenotype, suppressible by BRWD3ram1

NOT suppressed by
Statement
Reference

hhbar3/hh8 has visible phenotype, non-suppressible by BRWD3s5349/BRWD3[+]

Suppressor of
Statement
Reference

hh[+]/hh8 is a suppressor of visible phenotype of roDOM

Phenotype Manifest In
Enhanced by
Statement
Reference

hhbar3/hh8 has eye phenotype, enhanceable by snamaPX1/mnm[+]

hhbar3/hh8 has ommatidium phenotype, enhanceable by snamaPX1/mnm[+]

hhbar3/hh8 has eye phenotype, enhanceable by l(2)rQ313[+]/snamarQ313

hhbar3/hh8 has ommatidium phenotype, enhanceable by l(2)rQ313[+]/snamarQ313

hhbar3/hh8 has eye phenotype, enhanceable by snama1/mnm[+]

hhbar3/hh8 has ommatidium phenotype, enhanceable by snama1/mnm[+]

Suppressed by
Statement
Reference

hhbar3/hh8 has eye phenotype, suppressible by BRWD3ram1

hhbar3/hh8 has ommatidium phenotype, suppressible by BRWD3ram1

Suppressor of
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The presense of BRWD3s5349/+ does not significantly affect the size or roughness of eye in hh8/hhbar3 flies. However, retinal sections reveal the presence of small rhabdomeres in these eyes, which are not present in the eyes of hh8/hhbar3 animals in the absence of BRWD3s5349/+.

hh8 dominantly suppresses the amosRoi-1/+ phenotype; at least 50% of the ommatidia have normal structure and orientation in double heterozygotes.

Has no effect on the eye phenotype produced by activated arm constructs. (either armS44Y.GMR or armS56F.GMR).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

hhScer\UAS.cIa partially rescues the hh8 tracheal defects when expressed under the control of Scer\GAL4btl.PS. In particular, rescue of the dorsal trunk, visceral branches and some lateral trunk anterior branches is seen. The ectodermal defects are not rescued in these embryos.

The lack of Bolwig's organ neurons seen in hh8 embryos is partially rescued by hhScer\UAS.cKa expressed under the control of either Scer\GAL4h-1J3 or Scer\GAL4da.G32.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Allelic series in terms of phenotype severity: hh3 > hh8 > hh4.

Strong allele of hh.

Mutations in hh enhance the wg phenotype.

Strong hh allele, strength based on severity of ventral cuticle phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (42)