Allele Dmel\Hn^r3

General Information
Symbol	Dmel\Hn^r3	Species	D. melanogaster
Name	recessive-3	FlyBase ID	FBal0005505
Feature type	allele	Associated gene	Dmel\Hn

Map ( GBrowse )
Allele class
Mutagen	spontaneous
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	spontaneous
Mutations Mapped to the Genome

Type Location Additional Notes References sequence variant 3L:7,754,580..7,754,604 evidence=experimental comment=A 24 base deletion in Hn^r3 maps to an intron. The Hn^r3 allele also contains the ~7.2kb roo{}Hn^r3@ insertion. (Ruiz-Vazquez and Silva, 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AJ001717 AJ001718 CAA04950 AJ001720 AJ001721 AJ001722 CAB51599 AJ001723 CAB51597

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference roo element insertion (of at least 7.2kb) in exon 3. The roo element is inserted in the opposite orientation with respect to the Hn gene. A 24bp deletion has also occurred in intron 1. (Ruiz-Vazquez and Silva, 1999)
Caused by insertion	roo{}Hn^r3 (Ruiz-Vazquez and Silva, 1999)
Cytology
Phenotypic Data
Phenotypic Class
	eye color defective eye color defective (with Hn^bp) (Wang et al., 2008)
Phenotype Manifest In
	eye (with Hn^bp) (Wang et al., 2008) pigment cell
Detailed Description
	Statement Reference Hn[bp]/Hn[r3] flies have purple eyes. (Wang et al., 2008) Has 10% of wild type levels of phenylalanine hydroxylase enzyme activity and correspondingly reduced CRM. (Silva et al., 1992) Homozygotes of Hn^r3 and ry⁶ viable. Eye colour: dull chocolate; classification easier than for Hn^r. (Thompson, 1983) Seventy-nine percent normal red pigment, 100% normal brown pigment. (Nolte, 1955) Eye color autonomous in transplants of optic disk into wild-type hosts. (Beadle and Ephrussi, 1936) More extreme than Hn^r2.
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
Bloomington	519 Hn^r3 sr¹
Kyoto	105959 Hn^r3 sr¹ 106321 Dp(3;3)S2, ru¹ Hn^r3 h² app¹ cu¹ kar¹/In(3LR)HR33^Lbxd^106R, Sb^sbd-2 Ubx^bx-3
Notes on Origin
Discoverer	Weinstein, 1927.

Comments
	No detectable levels of Hn protein. (Alcaniz and Silva, 1997) Completely lacks 5,6,7,8-tetrahydroperin and increased levels of 5,6,7,8-tetrahydrobioperin. (Guillamon and Ferre, 1988)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Hn^r3 (Wang et al., 2008) sed
Name Synonym	recessive-3 (Guillamon and Ferre, 1988) sepiaoid
Secondary FlyBase IDs

References ( 12 )
Research paper	Wang et al., 2008, Sci. China C, Life Sci. 51(8): 702--710 Reduction of drosopterin content caused by a 45-nt insertion in Henna pre-mRNA of Drosophila melanogaster. [FBrf0205808] Ruiz-Vazquez and Silva, 1999, Insect Biochem. Mol. Biol. 29(4): 311--318 Aberrant splicing of the Drosophila melanogaster phenylalanine hydroxylase pre-mRNA caused by the insertion of a B104/roo transposable element in the Henna locus. [FBrf0108267] Alcaniz and Silva, 1997, Comp. Biochem. Physiol. C. Pharmacol. Toxicol. Endocrinol. 116(3): 205--212 Phenylalanine hydroxylase participation in the synthesis of serotonin and pteridines in Drosophila melanogaster. [FBrf0093933] Piedrafita et al., 1994, Insect Biochem. Mol. Biol. 24(6): 581--588 A genetic analysis of aromatic amino acid hydroxylases involvement in DOPA synthesis during Drosophila adult development. [FBrf0074167] Silva et al., 1992, Biochem. J. 287(1): 85--89 Immunological detection of phenylalanine hydroxylase protein in Drosophila melanogaster. [FBrf0055435] Silva et al., 1991, Mol. Gen. Genet. 230: 97--103 Genetic and biochemical characterization of little isoxanthopterin (lix), a gene controlling dihydropterin oxidase activity in Drosophila melanogaster. [FBrf0054724] Guillamon and Ferre, 1988, Biochem. Biophys. Res. Commun. 152(1): 49--55 Identification of 5,6,7,8-tetrahydropterin and 5,6,7,8-tetrahydrobiopterin in Drosophila melanogaster. [FBrf0047570] Thompson, 1983, D. I. S. 59: 128--129 Failure of the Hn^r3 ry⁶ combination to behave as a recessive synthetic lethal. [FBrf0039093] Lewis, 1956, D. I. S. 30: 130 Addition and corrections to the list of mutants in the work of Bridges-Brehme (1944). [FBrf0010688] Nolte, 1955, J. Genet. 53: 1--10 The eye-pigmentary system of Drosophila. [FBrf0010259] Beadle and Ephrussi, 1936, Genetics 21: 225--247 The differentiation of eye pigments in Drosophila as studied by transplantation. [FBrf0003530]
Book	Lindsley and Grell, 1968, Publs Carnegie Instn 627: 469pp Genetic variations of Drosophila melanogaster. [FBrf0020044]

Allele Dmel\Hnr3

Allele Dmel\Hn^r3