hop27 hemizygotes show a significant reduction in the size of the third instar larval wing disc, which is particularly evident on the posterior compartment, as compared to controls; second to late instar larval wing disc posterior compartments also show an obvious increase in the amount of apoptotic cells, as compared to either the anterior compartment or wild-type controls.
The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of hemizygous larvae is significantly higher than that of control siblings. The ability of hemizygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.
hop25/hop27 non-fused egg chambers sometimes have extra polar follicle cells (generally having 3 rather than the normal number of 2 polar cells at both the anterior and posterior ends of the egg chamber). At stage 8/9, 13% of egg chambers have extra polar cells at the anterior end and 36% of egg chambers have extra polar cells at the posterior end. The extra polar cells do not appear to be due to continued proliferation of the polar cell population. 5% of hop25/hop27 egg chambers are fused.
Paternal rescue of the maternal effect. Germline clone analysis demonstrates that embryos have one abdominal segment missing and A4 appears wider than wild type, and partial or complete fusion of T1 and T2.