Allele Dmel\hop²⁷

General Information
Symbol	Dmel\hop²⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0005538
Feature type	allele	Associated gene	Dmel\hop
Also Known As	hop^M4

Map ( GBrowse )
Allele class	amorphic allele - genetic evidence
Mutagen	ethyl nitrosourea
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Perrimon and Mahowald, 1986)
Mutagen	ethyl nitrosourea
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:11,256,488..11,256,488 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T11256488C pr_change=I1011T\|hop-PA reported_pr_change=I1011T (Luo et al., 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: I1011T. The mutation is within the kinase domain. (Luo et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| rescuable maternal effect (Perrimon and Mahowald, 1986) viable (with hop²⁵) (Harrison et al., 1998)
Phenotype Manifest In
	egg chamber (with hop²⁵) (McGregor et al., 2002) polar follicle cell \| supernumerary (with hop²⁵) (McGregor et al., 2002)
Detailed Description
	Statement Reference The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of hemizygous larvae is significantly higher than that of control siblings. The ability of hemizygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. (Sorrentino et al., 2004) hop²⁵/hop²⁷ non-fused egg chambers sometimes have extra polar follicle cells (generally having 3 rather than the normal number of 2 polar cells at both the anterior and posterior ends of the egg chamber). At stage 8/9, 13% of egg chambers have extra polar cells at the anterior end and 36% of egg chambers have extra polar cells at the posterior end. The extra polar cells do not appear to be due to continued proliferation of the polar cell population. 5% of hop²⁵/hop²⁷ egg chambers are fused. (McGregor et al., 2002) 100% viable in transheterozygous combination with hop²⁵. (Harrison et al., 1998) Paternal rescue of the maternal effect. Germline clone analysis demonstrates that embryos have one abdominal segment missing and A4 appears wider than wild type, and partial or complete fusion of T1 and T2. (Perrimon and Mahowald, 1986) larval/pupal lethal hop²⁷/hop²⁵ 100% viable
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference hop²⁷/hop[+] is an enhancer of visible phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006)
Other
Statement Reference hop²⁷, os^upd-4 has lethal \| recessive \| partially phenotype (Harrison et al., 1998)
Phenotype Manifest In
Enhanced by
Statement Reference hop²⁷/hop²⁵ has egg chamber phenotype, enhanceable by os[+]/os^upd-4 (McGregor et al., 2002) hop²⁷/hop²⁵ has egg chamber phenotype, enhanceable by os^upd-3/os[+] (McGregor et al., 2002) hop²⁷/hop²⁵ has polar follicle cell \| supernumerary phenotype, enhanceable by os[+]/os^upd-4 (McGregor et al., 2002)
Enhancer of
Statement Reference hop²⁷/hop[+] is an enhancer of wing vein \| ectopic phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference 75% of hop²⁵ os^upd-3/hop²⁷ egg chambers are fused. 92% of hop²⁵ os^upd-4/hop²⁷ egg chambers are fused and the number of extra polar cells is increased compared to hop²⁵/hop²⁷ single mutants. (McGregor et al., 2002) Viability of hop²⁷/hop²⁵ flies is reduced if the flies also carry os^upd-4. (Harrison et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	8493 y¹ hop²⁷/FM7c
Notes on Origin
Discoverer	Voelker.

Comments
	Fully complement hop²⁵. (Perrimon and Mahowald, 1986)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	hop²⁷ (Popodi et al., 2010-, Oishi et al., 2006) hop^M4 (Sorrentino et al., 2004, Oh et al., 2004, McGregor et al., 2002, Harrison et al., 1998) l(1)hop^M4 (Perrimon and Mahowald, 1986) l(1)M4 M4 (Luo et al., 1999)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Oishi et al., 2006, Hum. Mol. Genet. 15(4): 543--553 Transgenic Drosophila models of Noonan syndrome causing PTPN11 gain-of-function mutations. [FBrf0190828] Sorrentino et al., 2004, Genetics 166(3): 1343--1356 Genetic analysis of contributions of dorsal group and JAK-Stat92E pathway genes to larval hemocyte concentration and the egg encapsulation response in Drosophila. [FBrf0174690] McGregor et al., 2002, Development 129(3): 705--717 JAK signaling is somatically required for follicle cell differentiation in Drosophila. [FBrf0144841] Luo et al., 1999, Dev. Biol. 213(2): 432--441 The Drosophila JAK kinase hopscotch is required for multiple developmental processes in the eye. [FBrf0111419] Harrison et al., 1998, Genes Dev. 12(20): 3252--3263 Drosophila unpaired encodes a secreted protein that activates the JAK signaling pathway. [FBrf0105239] Perrimon and Mahowald, 1986, Dev. Biol. 118: 28--41 l(1)hopscotch, A larval-pupal zygotic lethal with a specific maternal effect on segmentation in Drosophila. [FBrf0044150]
Personal communication to FlyBase	Popodi et al., 2010-, Small X duplications for the stock center collection. Small X duplications for the stock center collection. [FBrf0210621]
Abstract	Oh et al., 2004, A. Dros. Res. Conf. 45: 490A Identify the targets of the HOP/STAT92E signaling in testis and ovary using the microarray technique. [FBrf0173457]

Allele Dmel\hop27

Allele Dmel\hop²⁷