FlyBase Allele Report: Dmel\hop[Tum]

(Bausek and Zeidler, 2014, Bina et al., 2010, Nam et al., 2008, Minakhina and Steward, 2006, Muller et al., 2005, Badenhorst et al., 2002, Luo et al., 2002, Braun et al., 1998)

melanotic mass | adult stage

(Kugler et al., 2011)

melanotic mass | heat sensitive

(Kimbrell et al., 2002)

peripheral nervous system

(Li et al., 2003)

primordial germ cell | ectopic

(Li et al., 2003)

primordial germ cell | increased number

(Li et al., 2003)

tracheal pit

(Li et al., 2003)

Detailed Description

Statement

Reference

hop^Tum heterozygous adults exhibit a range of small to large tumor frequencies.

(Tettweiler et al., 2019)

Heterozygous adult females contain melanotic tumours. The incidence of lamellocytes as a percentage of the total hemocytes is increased compared to wild type in hemolymph preparations from third instar heterozygous larvae.

(Kugler et al., 2011)

Mutant adults contain multiple, large melanotic tumours.

Mutant third instar larvae show an increase in the number of circulating hemocytes compared to controls.

(Bina et al., 2010)

The number of hemocytes is increased in hop^Tum mutant third instar larvae compared to wild type.

(Sinenko et al., 2010)

hop^Tum lymph glands display considerable numbers of lamellocytes when larvae are raised at 23, 25, or 27[o]C.

(Gao et al., 2009)

Lymph glands of hop^Tum/Y larvae at 72 hours of development show hyperplasia and extensive lamellocyte differentiation. Lamellocytes reproducibly appear in the mutant lymph glands at around 54-56 hours of development at 25[o]C. A large population of lamellocytes are seen in the mutant hemolymph at 72 hours of development, and lamellocytes are reproducibly seen in the mutant hemolymph before 56 hours of development at 25[o]C.

Heterozygous larvae show a massive overproduction of both lamellocytes and non-lamellocyte hemocytes in the hemolymph.

(Tokusumi et al., 2009)

Mutant larvae have a large number of lamellocytes within the circulating hemolymph.

(Tokusumi et al., 2009)

75% of mutant larvae are melanised.

(Nam et al., 2008)

Lymph glands from hop^Tum/Y animals are hypertrophic and produce increased numbers of hemocytes and lamellocytes.

(Sorrentino et al., 2007)

Heterozygous third instar larvae have melanotic nodules found in the hemocoel or in association with He-positive lymph glands. The melanotic nodules are surrounded by lamellocytes.

(Minakhina and Steward, 2006)

Mutant larvae show overproliferation of plasmatocytes in the primary lymph gland lobes and hemocytes aberrantly differentiate into lamellocytes in the secondary lymph gland lobes.

(Davidson et al., 2005)

Heterozygotes often have melanotic tumours.

(Muller et al., 2005)

hop^Tum is dominant semi-lethal at 29^oC (survival of females to adulthood is 48% of wild-type).

(Chen et al., 2003)

The proportion of completely melanised tumours by larva is significantly decreased in hop^Tum larvae parasitised by the G431 L.boulardi strain compared to unparasitised hop^Tum larvae, while the proportion of partially melanised tumours is significantly increased.

(Labrosse et al., 2003)

hop^Tum embryos have nearly twice as many pole cells as wild-type embryos. At stage 3, the pole cells in hop^Tum mutants change shape and migrate away from the posterior pole, while pole cells in wild-type embryos remain a spherical shape and are confined to the posterior area. Pole cells in the mutants are frequently observed to prematurely transit through the gut and migrate errantly. Such pole cells survive, in contrast to wild-type embryos where errant pole cells are destroyed by apoptosis, and aggregate in ectopic locations. Pole cells from cellularization stage hop^Tum embryos are more active both in dispersion and translocation movements when observed in Schneider culture medium, than pole cells from wild-type embryos.

(Li et al., 2003)

In mutants the tracheal pits are larger than in wild-type. The hindgut is also longer than seen in wild-type. The hindgut nuclei in mutants form a single layer and are further apart from each other, giving the appearance of being stretched. The peripheral nervous system of mutants differentiate and grow axons prematurely - the PNS is nearly fully differentiated at stage 12 (as compared to 15 in wild-type).

(Li et al., 2003)

About a quarter of heterozygous mutant larvae exhibit melanotic tumours when grown at 25^oC about half exhibit tumours when grown at 28^oC.

(Badenhorst et al., 2002)

Mutant larvae raised at the non-permissive temperature (29^oC) have melanotic tumours.

(Kimbrell et al., 2002)

At 28^oC, hop^Tum larvae show a six-fold increase in the concentration of circulating blood cells compared to wild-type larvae. 48% of the plasmatocytes are differentiated into lamellocytes in hop^Tum larvae compared to 6% in wild-type larvae. The larvae have overgrown and melanised lymph glands.

(Luo et al., 2002)

The concentration of circulating hemocytes in hemizygous larvae is increased compared to controls. The distribution of blood cell types is altered compared to wild type; in the mutant larvae 63.2% are plasmatocytes (more than 99% in controls), 11.4% are podocytes (less than 0.1% in controls) and 25.4% are lamellocytes (0.1% in controls).

(Remillieux-Leschelle et al., 2002)

100% of hemizygous third instar larvae show multiple melanotic capsules at 18^oC, 25^oC and 29^oC. Hemizygous adults show multiple melanotic capsules at 25^oC.

(Sorrentino et al., 2002)

At 25^oC 37% of heterozygous mutant adult females have at least one abdominal tumour.

(Betz et al., 2001)

When raised at 25^oC, second instar mutant larvae have abundant (10-15%) lamellocytes in circulation and in some larvae lamellocytes can be seen in lymph gland lobes. Melanotic tumours are not yet present. Lamellocyte numbers increase very rapidly in the course of the third instar stage and but the end of the instar represent close to 50% of the circulating blood cell population. Plasmatocytes frequently show abnormal oversized features. Lamellocytes are very abundant in the lymph glands before pupariation. Crystal cells are rarely round in mutant larvae.

(Lanot et al., 2001)

Larvae contain free-floating melanotic masses.

(Braun et al., 1998)

Heterozygotes grown above 25^oC have reduced viability and develop melanotic tumors.

(Hou et al., 1996)

At high culture temperatures, abnormalities in hemocyte proliferation and differentiation, melanization of hematopoetic and gut tissue and recessive lethality result.

(Yan et al., 1996)

Formation of melanotic tumours and hypertrophy of the lymph glands. Tissue transplantation indicates the lymph glands can autonomously generate melanotic masses associated with the mutation.

(Harrison et al., 1995)

Homozygotes raised at 29^oC form black melanotic masses, haematopoietic defects. Lymph gland material transplanted into a wild type host that is cultured at 29^oC causes the appearance of melanotic masses in the abdominal cavity and/or in the leg joints, bloating is also observed. Elevated levels of lamellocytes are found in the haemolymph due to overproliferation and premature differentiation.

(Luo et al., 1995)

Blood cell neoplastic phenotype.

(Mechler, 1994)

Hematopoietic neoplasms, overproliferation of hemocyte stem cells in the larval lymph gland and premature differentiation of circulating haemocytes. Lethality occurs at 29^oC.

(Hanratty and Dearolf, 1993)

Dominant tumorous gene that is a temperature-sensitive lethal at 29^oC in hemizygous males and homozygous females; about two-thirds of the hemizygous males survive at 18^oC and one-quarter of these have melanotic tumors. Males raised at 26^oC and heterozygous females raised at 29^oC survive to adulthood, but show melanotic masses in the abdominal cavity or small black specks in the legs, wings, or thorax. Mutant larvae kept at 29^oC show enlargement of the lymph glands in the late second- or early third instar larvae, but no melanotic masses. By mid third instar, the lymph glands are large and diffuse and the gastric caeca have become encapsulated and melanized. By late third instar, the larvae have melanotic masses in the body cavity, lack lymph glands and have reduced, encapsulated and melanized gastric caeca as well as encapsulated and melanized muscles and fat bodies. These mutants do not survive beyond the late third instar or the early pupal stage. When lymph glands from Tum larvae are injected into adult female hosts, transplantable neoplasms are produced. Melanization, at first associated with the leg joints and later with the head, thorax and abdomen, takes place; also abdominal bloating. The lymph glands become melanotic and the abdomen is filled with encapsulated masses before the premature death of the injected individuals. Injection of Tum tissue other than lymph glands fails to produce these effects. The melanotic neoplasms can be transplanted into a succession of hosts in which they produce the same abnormalities. The neoplastic cells resemble hemocytes; some cell lines are melanotic and others are unpigmented, but in both types, the tissue, when transplanted, grows rapidly in the hosts and kills them.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hop^Tum has melanotic mass phenotype phenotype, enhanceable by Scer\GAL4^dome-PG5/ush^VDRC.cUa

(Bausek and Zeidler, 2014)

hop^Tum has melanotic mass phenotype phenotype, enhanceable by Scer\GAL4^dome-PG5/Gαf^UAS.cBa

(Bausek and Zeidler, 2014)

hop^Tum has melanotic mass phenotype | adult stage | dominant phenotype, enhanceable by pzg⁶⁶/pzg[+]

(Kugler et al., 2011)

hop^Tum has melanotic mass phenotype | adult stage | dominant phenotype, enhanceable by E(bx)^Nurf301-2/E(bx)[+]

(Kugler et al., 2011)

hop^Tum has melanotic mass phenotype phenotype, enhanceable by mfas^GD5174/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, enhanceable by CG10764^GD5293/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, enhanceable by Scer\GAL4^dome-PG5/pxb^GD8472

(Bina et al., 2010)

hop^Tum has neoplasia | dominant phenotype, enhanceable by Df(3L)ED4238/+

(Baeg et al., 2005)

hop^Tum has neoplasia phenotype, enhanceable by Df(2R)w45-30n

(Betz et al., 2001)

hop^Tum has neoplasia phenotype, enhanceable by Su(var)2-10⁰³⁶⁹⁷

(Betz et al., 2001)

hop^Tum has lethal phenotype, enhanceable by E(hop^Tum)¹

(Zinyk et al., 1993)

NOT Enhanced by

Statement

Reference

hop^Tum has melanotic mass phenotype | adult stage phenotype, non-enhanceable by Scer\GAL4^Hml.Δ/Hipk^UAS.Tag:HA

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype | adult stage phenotype, non-enhanceable by Hipk^{UAS.Tag:HA,Tag:Myr(v-Src)}/Scer\GAL4^Hml.Δ

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype | adult stage phenotype, non-enhanceable by Scer\GAL4^Hml.Δ/Hipk^{UAS.Tag:HA,Tag:NLS(Unk)}

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Scer\GAL4^dome-PG5/Rh4^GD17228

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Scer\GAL4^dome-PG5/w^GD14981

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Idgf1^GD5405/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Efa6^GD14945/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Scer\GAL4^dome-PG5/rgn^GD5549

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Scer\GAL4^dome-PG5/CG4080^GD2389

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by bez^GD2224/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by l(2)gl^GD4047/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Scer\GAL4^dome-PG5/P{GD17521}

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by net^GD4378/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Scer\GAL4^dome-PG5/Wnt4^GD5347

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-enhanceable by Scer\GAL4^dome-PG5/pirk^GD9084

(Bina et al., 2010)

Suppressed by

Statement

Reference

hop^Tum has melanotic mass phenotype | adult stage phenotype, suppressible by Scer\GAL4^Hml.Δ/Hipk^KK107857

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype | larval stage phenotype, suppressible | partially by Hipk[+]/Hipk⁴

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Gαf^c05874/Gαf^c05874

(Bausek and Zeidler, 2014)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Stat92E^GD4492/Scer\GAL4^dome-PG5

(Bausek and Zeidler, 2014, Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Scer\GAL4^dome-PG5/CG15211^GD13281

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Scer\GAL4^dome-PG5/baz^GD1384

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Scer\GAL4^dome-PG5/baz^UAS.cKa

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Gαf^GD7540/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Scer\GAL4^dome-PG5/CG4793^GD5233

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Scer\GAL4^dome-PG5/CG1572^GD13286

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Scer\GAL4^dome-PG5/CG13559^GD2179

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype | larval stage phenotype, suppressible by Scer\GAL4^da.G32/PPO3^RNAi.UAS

(Nam et al., 2008)

hop^Tum has increased cell number | larval stage phenotype, suppressible by Myb^MH107

(Davidson et al., 2005)

Scer\GAL4^Cg.PA, hop^Tum has melanotic mass phenotype | dominant phenotype, suppressible by Ptp61F^c.UAS

(Muller et al., 2005)

hop^Tum has melanotic mass phenotype | dominant phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Muller et al., 2005)

hop^Tum has melanotic mass phenotype | dominant phenotype, suppressible by BRWD3⁰⁵⁸⁴²/BRWD3[+]

(Muller et al., 2005)

hop^Tum has partially lethal - majority die | dominant | heat sensitive phenotype, suppressible by CycE[+]/CycE⁰⁵²⁰⁶

(Chen et al., 2003)

hop^Tum has partially lethal - majority die | dominant | heat sensitive phenotype, suppressible by Cdk4³/Cdk4[+]

(Chen et al., 2003)

hop^Tum has increased cell number | third instar larval stage phenotype, suppressible by Dsor1^S-1221

(Luo et al., 2002)

hop^Tum has increased cell number | third instar larval stage phenotype, suppressible by Dsor1^XS520

(Luo et al., 2002)

hop^Tum has abnormal immune response | heat sensitive phenotype, suppressible by ecd¹

(Sorrentino et al., 2002)

hop^Tum has melanotic mass phenotype | heat sensitive phenotype, suppressible by ecd¹

(Sorrentino et al., 2002)

hop^Tum has neoplasia phenotype, suppressible by Su(var)2-10^537.UAS/Scer\GAL4^hs.PB

(Betz et al., 2001)

hop^Tum has partially lethal phenotype, suppressible by Stat92E⁰⁶³⁴⁶

(Hou et al., 1996)

hop^Tum has melanotic mass phenotype phenotype, suppressible by Stat92E⁰⁶³⁴⁶

(Hou et al., 1996)

hop^Tum has lethal phenotype, suppressible by awd^K

(Zinyk et al., 1993)

NOT suppressed by

Statement

Reference

hop^Tum has lethal | heat sensitive phenotype, non-suppressible by Hipk[+]/Hipk⁴

(Tettweiler et al., 2019)

hop^Tum has lethal | heat sensitive phenotype, non-suppressible by Scer\GAL4^Hml.Δ/Hipk^KK107857

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype | adult stage phenotype, non-suppressible by Scer\GAL4^Hml.Δ/Hipk^UAS.Tag:HA

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype | adult stage phenotype, non-suppressible by Hipk^{UAS.Tag:HA,Tag:Myr(v-Src)}/Scer\GAL4^Hml.Δ

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype | adult stage phenotype, non-suppressible by Scer\GAL4^Hml.Δ/Hipk^{UAS.Tag:HA,Tag:NLS(Unk)}

(Tettweiler et al., 2019)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Scer\GAL4^dome-PG5/Rh4^GD17228

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Scer\GAL4^dome-PG5/w^GD14981

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Idgf1^GD5405/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Efa6^GD14945/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Scer\GAL4^dome-PG5/rgn^GD5549

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Scer\GAL4^dome-PG5/CG4080^GD2389

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by bez^GD2224/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by l(2)gl^GD4047/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Scer\GAL4^dome-PG5/P{GD17521}

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by net^GD4378/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Scer\GAL4^dome-PG5/Wnt4^GD5347

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Scer\GAL4^dome-PG5/pirk^GD9084

(Bina et al., 2010)

hop^Tum has increased cell number | third instar larval stage phenotype, non-suppressible by EcR[+]/EcR^KG04522

(Sinenko et al., 2010)

hop^Tum has increased cell number | third instar larval stage phenotype, non-suppressible by EcR[+]/EcR^Q50st

(Sinenko et al., 2010)

Scer\GAL4^Cg.PA, hop^Tum has melanotic mass phenotype | dominant phenotype, non-suppressible by Ptp61F^a.UAS

(Muller et al., 2005)

hop^Tum has hyperplasia | third instar larval stage phenotype, non-suppressible by Dsor1^S-1221

(Luo et al., 2002)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Dsor1^S-1221

(Luo et al., 2002)

hop^Tum has melanotic mass phenotype phenotype, non-suppressible by Raf¹¹

(Luo et al., 2002)

hop^Tum has increased occurrence of cell division phenotype, non-suppressible by Stat92E⁰⁶³⁴⁶

(Luo et al., 1997)

hop^Tum has increased occurrence of cell division phenotype, non-suppressible by Stat92E^HJ

(Luo et al., 1997)

Suppressor of

Statement

Reference

hop^Tum/hop[+] is a suppressor | partially of visible | P-stage phenotype of Ipk2^20B

(Seeds et al., 2015)

hop^Tum/hop[+] is a suppressor | partially of hypoplasia | P-stage phenotype of Ipk2^20B

(Seeds et al., 2015)

hop^Tum/hop[+] is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

hop^Tum is a suppressor of visible phenotype of Scer\GAL4^ey.PH, Su(var)2-10^537.UAS

(Betz et al., 2001)

NOT Suppressor of

Statement

Reference

hop^Tum/hop[+] is a non-suppressor of lethal - all die before end of P-stage phenotype of Ipk2^20B

(Seeds et al., 2015)

Other

Statement

Reference

α-Man-IIb^MI09613, hop^Tum has visible | adult stage phenotype

(Mortimer et al., 2021)

Mgat1^KG02444, hop^Tum has visible | adult stage phenotype

(Mortimer et al., 2021)

Mgat1^KG02444, hop^Tum has melanotic mass phenotype | adult stage phenotype

(Mortimer et al., 2021)

α-Man-IIb^MI09613, hop^Tum has melanotic mass phenotype phenotype

(Mortimer et al., 2021)

Rho1^N19.UAS, Scer\GAL4^dome-PG5, hop^Tum has lethal phenotype

(Bausek and Zeidler, 2014)

Rho1^V14.UAS, Scer\GAL4^dome-PG5, hop^Tum has lethal phenotype

(Bausek and Zeidler, 2014)

Df(3L)ED4238/+, hop^Tum has partially lethal phenotype

(Baeg et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

hop^Tum has melanotic mass phenotype, enhanceable by Scer\GAL4^dome-PG5/ush^VDRC.cUa

(Bausek and Zeidler, 2014)

hop^Tum has melanotic mass phenotype, enhanceable by Scer\GAL4^dome-PG5/Gαf^UAS.cBa

(Bausek and Zeidler, 2014)

hop^Tum has melanotic mass | adult stage phenotype, enhanceable by pzg⁶⁶/pzg[+]

(Kugler et al., 2011)

hop^Tum has lamellocyte | larval stage | increased number phenotype, enhanceable by pzg⁶⁶/pzg[+]

(Kugler et al., 2011)

hop^Tum has melanotic mass | adult stage phenotype, enhanceable by E(bx)^Nurf301-2/E(bx)[+]

(Kugler et al., 2011)

hop^Tum has melanotic mass phenotype, enhanceable by mfas^GD5174/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, enhanceable by CG10764^GD5293/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, enhanceable by Scer\GAL4^dome-PG5/pxb^GD8472

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, enhanceable by E(bx)^Nurf301-2

(Badenhorst et al., 2002)

hop^Tum has melanotic mass phenotype, enhanceable by Df(3L)3643

(Badenhorst et al., 2002)

NOT Enhanced by

Statement

Reference

hop^Tum has melanotic mass phenotype, non-enhanceable by Scer\GAL4^dome-PG5/Rh4^GD17228

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Scer\GAL4^dome-PG5/w^GD14981

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Idgf1^GD5405/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Efa6^GD14945/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Scer\GAL4^dome-PG5/rgn^GD5549

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Scer\GAL4^dome-PG5/CG4080^GD2389

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by bez^GD2224/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by l(2)gl^GD4047/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Scer\GAL4^dome-PG5/P{GD17521}

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by net^GD4378/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Scer\GAL4^dome-PG5/Wnt4^GD5347

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Scer\GAL4^dome-PG5/pirk^GD9084

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by TotA^GD6210/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-enhanceable by Spn31A^GD5369/Scer\GAL4^dome-PG5

(Bina et al., 2010)

Suppressed by

Statement

Reference

hop^Tum has melanotic mass phenotype, suppressible by Gαf^c05874/Gαf^c05874

(Bausek and Zeidler, 2014)

hop^Tum has melanotic mass phenotype, suppressible by Stat92E^GD4492/Scer\GAL4^dome-PG5

(Bausek and Zeidler, 2014, Bina et al., 2010)

hop^Tum has embryonic/larval hemocyte | increased number phenotype, suppressible by Scer\GAL4^dome-PG5/baz^UAS.cKa

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, suppressible by Scer\GAL4^dome-PG5/CG15211^GD13281

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, suppressible by Scer\GAL4^dome-PG5/baz^GD1384

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, suppressible by Scer\GAL4^dome-PG5/baz^UAS.cKa

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, suppressible by Gαf^GD7540/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, suppressible by Scer\GAL4^dome-PG5/CG4793^GD5233

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, suppressible by Scer\GAL4^dome-PG5/CG1572^GD13286

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, suppressible by Scer\GAL4^dome-PG5/CG13559^GD2179

(Bina et al., 2010)

hop^Tum has lamellocyte | increased number phenotype, suppressible by kay¹/kay[+]

(Tokusumi et al., 2009)

hop^Tum has melanotic mass phenotype, suppressible by Scer\GAL4^da.G32/PPO3^RNAi.UAS

(Nam et al., 2008)

hop^Tum has lamellocyte phenotype, suppressible by Scer\GAL4^Cg.PA/ush^UAS.cFa

(Sorrentino et al., 2007)

Scer\GAL4^Cg.PA, hop^Tum has melanotic mass phenotype, suppressible by Ptp61F^c.UAS

(Muller et al., 2005)

hop^Tum has melanotic mass phenotype, suppressible by BRWD3⁰⁵⁸⁴²/BRWD3[+]

(Muller et al., 2005)

hop^Tum has melanotic mass phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Muller et al., 2005)

hop^Tum has hemocyte | increased number phenotype, suppressible by Dsor1^S-1221

(Luo et al., 2002)

hop^Tum has hemocyte | increased number phenotype, suppressible by Dsor1^XS520

(Luo et al., 2002)

hop^Tum has hemocyte | increased number phenotype, suppressible by Raf¹¹

(Luo et al., 2002)

hop^Tum has lamellocyte | increased number phenotype, suppressible by Dsor1^S-1221

(Luo et al., 2002)

hop^Tum has lamellocyte | increased number phenotype, suppressible by Dsor1^XS520

(Luo et al., 2002)

hop^Tum has lamellocyte | increased number phenotype, suppressible by Raf¹¹

(Luo et al., 2002)

hop^Tum has lamellocyte phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Remillieux-Leschelle et al., 2002)

hop^Tum has embryonic/larval hemocyte phenotype, suppressible by brm[+]/brm²

(Remillieux-Leschelle et al., 2002)

hop^Tum has embryonic/larval plasmatocyte phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Remillieux-Leschelle et al., 2002)

hop^Tum has embryonic/larval hemocyte phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Remillieux-Leschelle et al., 2002)

hop^Tum has phenotype, suppressible by Stat92E^HJ

(Luo et al., 1997, Yan et al., 1996, Yan et al., 1996)

hop^Tum has phenotype, suppressible by Stat92E⁰⁶³⁴⁶

(Luo et al., 1997)

hop^Tum has melanotic mass phenotype, suppressible by awd^K

(Zinyk et al., 1993)

hop^Tum has hemocyte phenotype, suppressible by awd^K

(Zinyk et al., 1993)

NOT suppressed by

Statement

Reference

hop^Tum has melanotic mass phenotype, non-suppressible by Scer\GAL4^dome-PG5/Rh4^GD17228

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Scer\GAL4^dome-PG5/w^GD14981

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Idgf1^GD5405/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Efa6^GD14945/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Scer\GAL4^dome-PG5/rgn^GD5549

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Scer\GAL4^dome-PG5/CG4080^GD2389

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by bez^GD2224/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by l(2)gl^GD4047/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Scer\GAL4^dome-PG5/P{GD17521}

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by net^GD4378/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Scer\GAL4^dome-PG5/Wnt4^GD5347

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Scer\GAL4^dome-PG5/pirk^GD9084

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by TotA^GD6210/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has melanotic mass phenotype, non-suppressible by Spn31A^GD5369/Scer\GAL4^dome-PG5

(Bina et al., 2010)

hop^Tum has embryonic/larval hemocyte | third instar larval stage phenotype, non-suppressible by EcR[+]/EcR^KG04522

(Sinenko et al., 2010)

hop^Tum has embryonic/larval hemocyte | third instar larval stage phenotype, non-suppressible by EcR[+]/EcR^Q50st

(Sinenko et al., 2010)

Scer\GAL4^Cg.PA, hop^Tum has melanotic mass phenotype, non-suppressible by Ptp61F^a.UAS

(Muller et al., 2005)

hop^Tum has melanotic mass phenotype, non-suppressible by Dsor1^S-1221

(Luo et al., 2002)

hop^Tum has melanotic mass phenotype, non-suppressible by Raf¹¹

(Luo et al., 2002)

hop^Tum has embryonic/larval lymph gland phenotype, non-suppressible by Dsor1^S-1221

(Luo et al., 2002)

hop^Tum has embryonic/larval lymph gland phenotype, non-suppressible by Raf¹¹

(Luo et al., 2002)

hop^Tum has lamellocyte phenotype, non-suppressible by brm[+]/brm²

(Remillieux-Leschelle et al., 2002)

Suppressor of

Statement

Reference

hop^Tum/hop[+] is a suppressor | partially of wing | P-stage phenotype of Ipk2^20B

(Seeds et al., 2015)

hop^Tum/hop[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

hop^Tum is a suppressor of embryonic/larval posterior spiracle phenotype of dome^G0441

(Chen et al., 2002)

hop^Tum is a suppressor of eye phenotype of Scer\GAL4^ey.PH, Su(var)2-10^537.UAS

(Betz et al., 2001)

hop^Tum is a suppressor of wing vein | ectopic phenotype of Stat92E^HJ

(Luo et al., 1997, Yan et al., 1996)

Other

Statement

Reference

α-Man-IIb^MI09613, hop^Tum has adult fat body | adult stage phenotype

(Mortimer et al., 2021)

Mgat1^KG02444, hop^Tum has adult fat body | adult stage phenotype

(Mortimer et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

hop^Tum/+ partially suppresses the small wing phenotype but does not suppress lethality in Ipk2^20B/Ipk2^20B pupae.

(Seeds et al., 2015)

The penetrance of the melanotic mass phenotype seen in hop^Tum/+ females is enhanced if they also carry one copy of pzg⁶⁶ or of E(bx)^Nurf301-2.

The incidence of lamellocytes as a percentage of the total hemocytes in hemolymph preparations is increased in hop^Tum/+ ; pzg⁶⁶/+ larvae compared to hop^Tum/+ larvae.

(Kugler et al., 2011)

Expression of baz^Scer\UAS.cKa under the control of Scer\GAL4^dome-PG5 reduces the number of circulating hemocytes in hop^Tum third instar larvae.

(Bina et al., 2010)

One copy of EcR^KG04522 is unable to suppress the hemocyte overproliferation seen in hop^Tum mutant third instar larvae.

One copy of EcR^Q50st is unable to suppress the hemocyte overproliferation seen in hop^Tum mutant third instar larvae.

(Sinenko et al., 2010)

kay¹/+ significantly suppresses the number of lamellocytes in the hemolymph of hop^Tum/+ larvae.

(Tokusumi et al., 2009)

The frequency of melanisation seen in hop^Tum larvae is reduced (from 75% to 28% of larvae) when they are also expressing CG42640^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32.

(Nam et al., 2008)

Lymph glands in hop^Tum/Y animals expressing ush^Scer\UAS.cFa under the control of Scer\GAL4^Cg.PA are hypertrophic and produce lamellocytes, although the number of circulating lamellocytes is reduced by 90%.

(Sorrentino et al., 2007)

Removing one copy of Ptp61F, with the Df(3L)ED4238 deficiency, in hop^Tum heterozygous females leads to a significant decrease in the survival rate and a dramatic enhancement in the formation of melanotic tumors (89.83% of flies with tumors compared to 19.43% of hop^Tum/+ flies).

(Baeg et al., 2005)

Hemocytes in the secondary lymph gland lobes of hop^Tum Myb^MH107 double mutant larvae fail to overproliferate and do not adopt the flattened shape characteristic of differentiated lamellocytes.

(Davidson et al., 2005)

One copy of BRWD3⁰⁵⁸⁴² or Stat92E⁰⁶³⁴⁶ reduces the size and the frequency of the melanotic tumours induced by hop^Tum/+. Expression of Ptp61F^c.Scer\UAS under the control of Scer\GAL4^Cg.PA suppresses the formation of melanotic tumours that is seen in a hop^Tum/+ background. Expression of Ptp61F^a.Scer\UAS under the control of Scer\GAL4^Cg.PA does not suppress the formation of melanotic tumours that is seen in a hop^Tum/+ background.

(Muller et al., 2005)

Survival to adulthood of hop^Tum/+ females raised at 29^oC is restored to 96% wild-type levels by heterozygosity for Cdk4³ and to 85% by heterozygosity for CycE⁰⁵²⁰⁶.

(Chen et al., 2003)

The addition of Df(3L)3643 or E(bx)^Nurf301-2 enhances the melanotic tumour phenotype seen in hop^Tum/+ larvae, so that over half exhibit tumours at 25^oC and 3/4 at 28^oC (in the case of Df(3L)3643/+) and over 60% exhibit tumours at 25^oC and 80% at 28^oC, in the case of E(bx)^Nurf301-2.

(Badenhorst et al., 2002)

At 28^oC, less than 3% lamellocytes are seen in phl¹² hop^Tum larvae. Plasmatocytes with abnormal morphology are occasionally seen in these animals; some blood cells are larger than the plasmatocytes normally seen in wild-type larvae, while others have processes or an elongated morphology. The overproliferation of blood cells seen in hop^Tum larvae is not suppressed by phl¹². phl¹² hop^Tum larvae have reduced melanotic tumour formation compared to hop^Tum larvae, but the lymph glands are still overgrown. At 28^oC, the increased differentiation of blood cells into lamellocytes and the increased accumulation of blood cells seen in hop^Tum larvae is completely suppressed by phl¹¹. All hemizygous phl¹¹ hop^Tum larvae have overgrown and melanised lymph glands, similar to that seen in hop^Tum mutants cultured at 28^oC. Dsor1^S-1221 or Dsor1^XS520 block differentiation of hop^Tum blood cells into lamellocytes and the increased accumulation of blood cells seen in hop^Tum larvae is strongly inhibited in the double mutant larvae. Dsor1^S-1221 hop^Tum mutant lymph glands are overgrown and melanised.

(Luo et al., 2002)

mxc^G43 hop^Tum/Y lymph glands contain differentiated hemocytes. The increased concentration of circulating hemocytes seen in hop^Tum larvae is significantly reduced by brm²/+, although lamellocyte ratios remain unchanged in the double mutants.

(Remillieux-Leschelle et al., 2002)

Lowering of ecdysone levels in hop^Tum/Y; ecd¹/ecd¹ resulted in mild suppression of the hop^Tum melanotic capsule phenotype and the encapsulation capacity on parasitisation by L. boulardi G486.

(Sorrentino et al., 2002)

The addition of Su(var)2-10⁰³⁶⁹⁷ or Df(2R)Np3 enhances the tumor phenotype seen in hop^Tum flies, over doubling the frequency of flies with at least one tumour. The addition of Su(var)2-10^537.Scer\UAS driven by Scer\GAL4^hs.PB significantly suppresses the tumourigenic phenotype of hop^Tum, decreasing the proportion of flies with tumours to about half.

(Betz et al., 2001)

hop^Tum; dom^k08108 double mutant larvae contain melanotic masses, although their frequency is markedly reduced compared to hop^Tum single mutant larvae and the masses are devoid of hemocytes.

(Braun et al., 1998)

Stat92E⁰⁶³⁴⁶ and Stat92E^HJ can suppress the premature differentiation and aggregation but not the overproliferation of larval haemocytes.

(Luo et al., 1997)

Suppresses the ectopic wing vein phenotype of Stat92E^HJ.

(Yan et al., 1996)

Lethality of hop^Tum rescued by awd^K alleviating premature formation of circulating haemocytes and melanotic mass formation in temperature- dependent fashion.

(Zinyk et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8492

y¹ v¹ hop^Tum/FM7c

Notes on Origin

Discoverer

Comments

Encodes an activated form of hop.

(Harrison et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

Hop^Tum-l

(Han and Harrison, 2007, Han and Harrison, 2006, Li et al., 2003, Hoffmann and Reichhart, 1997)

Hop^Tum1

(Khadilkar et al., 2017, Silver and Montell, 2001)

Tum

Tum-1

(Dostert et al., 2005, Mathey-Prevot and Perrimon, 1998)

Tum-l

(Agaisse and Perrimon, 2004, Tirouvanziam et al., 2004, Luo, 2002, Hou et al., 1996, Hanratty and Dearolf, 1993)

Tum¹

(Gateff and Mechler, 1989)

Tum^L

(Briscoe et al., 1996)

Tum^l

(Myrick and Dearolf, 2000, Mechler, 1994)

TumL

(Agaisse et al., 2003, Hultmark and Ekengren, 2003)

hop^GOF

(Li et al., 2003)

hop^TUM

(Doherty et al., 2014)

hop^Tum-1

(Yang et al., 2019, Walker et al., 2011, Gao et al., 2009, Gutierrez-Aviño et al., 2009, Stofanko et al., 2008, Sorrentino et al., 2007, Baeg et al., 2005, Chiu et al., 2005, Chen et al., 2003, Evans et al., 2003, Labrosse et al., 2003, Badenhorst et al., 2002, Lanot et al., 2001, Jinks et al., 2000, Zeidler et al., 2000, Dearolf, 1999)

hop^Tum-l

(Huang et al., 2023, Boulet et al., 2021, Yadav et al., 2021, Panettieri et al., 2019, Tettweiler et al., 2019, Anderson et al., 2017, Singh et al., 2016, Seeds et al., 2015, Kugler et al., 2011, Gutierrez-Aviño et al., 2009, Tokusumi et al., 2009, Tokusumi et al., 2009, Nam et al., 2008, Bach et al., 2007, Guo and Harrison, 2007, Shen and Tanda, 2007, Arbouzova and Zeidler, 2006, Sorrentino et al., 2004, Baksa et al., 2002, Chen et al., 2002, Kimbrell et al., 2002, Luo et al., 2002, Munier et al., 2002, Remillieux-Leschelle et al., 2002, Sorrentino et al., 2002, Betz et al., 2001, Luo and Dearolf, 2001, Barillas-Mury et al., 2000, Chiu and Govind, 2000, Myrick and Dearolf, 2000, Govind et al., 1999, Luo et al., 1999, Myrick and Dearolf, 1999, Sorrentino et al., 1999, Braun et al., 1998, Dearolf, 1998, Myrick and Dearolf, 1998, Braun et al., 1997, Hou and Perrimon, 1997, Luo et al., 1997, Luo et al., 1996, Yan et al., 1996, Harrison et al., 1995, Luo et al., 1995, Watson et al., 1994)

hop^Tum1

(Sinenko et al., 2010, Zeidler et al., 1999)

hop^Tum

(Bhaskar et al., 2022, Kúthy-Sutus et al., 2022, Mortimer et al., 2021, Bailetti et al., 2019, Tettweiler et al., 2019, Stratoulias and Heino, 2015, Seong et al., 2014, Novakova and Dolezal, 2011, Buchon et al., 2009, Habayeb et al., 2009, Kwon et al., 2008, Brun et al., 2006, Minakhina and Steward, 2006, Oishi et al., 2006)

hop^TumL

(Muller, 2005, Li et al., 2003)

hop^Tuml

(Bausek and Zeidler, 2014, Bina et al., 2010, Lam et al., 2010, Davidson et al., 2005, Muller et al., 2005)

hop^tum-1

(Chen et al., 2018, Lagueux et al., 2000)

hopTum-l

(Rennebeck et al., 2002)

hop[Tum-l]

(Sorrentino et al., 2006)

tum-1

(de la Rosa et al., 1995)

Name Synonyms

Tumorous

Tumorous lethal

(Kimbrell and Beutler, 2001)

Tumorous-lethal

(Agaisse and Perrimon, 2004, Hanratty and Dearolf, 1993)

Secondary FlyBase IDs

References (115)

Report Sections

Open Close