Allele Dmel\in¹

General Information
Symbol	Dmel\in¹	Species	D. melanogaster
Name		FlyBase ID	FBal0005591
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\in

Allele class	amorph
Mutagen	spontaneous
Nature of the Allele
Allele class	amorph (Adler et al., 1994)
Mutagen	spontaneous (Adler et al., 1994)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode
Carried on aberration	Dp(3;3)Cam36 (Roote, 2000.3.14, Roote, 2004.6.24)
Cytology	Polytene chromosomes normal.
Phenotypic Data
Phenotypic Class
	visible \| conditional - cold sensitive (Adler et al., 1994) tissue polarity defective (Lee and Adler, 2002)
Phenotype Manifest In
	wing cell (Wong and Adler, 1993) wing hair (Wong and Adler, 1993) microchaeta & wing (Park et al., 1996) macrochaeta \| conditional - cold sensitive (Adler et al., 1994) wing hair & 1st posterior cell (Lee and Adler, 2002) macrochaeta & adult thorax (Lee and Adler, 2002) microchaeta & adult thorax (Lee and Adler, 2002) microchaeta & leg (Lee and Adler, 2002) bract (Lee and Adler, 2002)
Detailed Description
	Statement Reference Hairs and bristles on thorax and abdominal tergites, directed irregularly toward midline; trichomes and chaetae on mesonotum coordinately misdirected (Toney and Thompson, 1980). Marginal hairs of wing stand out from wing margin; trichomes on wing blade no longer directed in parallel with longitudinal veins; frequently divergent therefrom. 50-60% of trichome-bearing cells express duplicated or rarely triplicated trichomes. Wings slightly spread and tend to be long and narrow. Somatic clones of homozygous cells in wing described by Gubb and Garcia-Bellido. Incomplete extra leg joints tend to form as mirror-image duplications proximal to the normal joints on tarsal segments three and four (Held, Duarte and Derakhshanian, 1986). RK1. Many wing cells of homozygous flies form two or three hairs. Homozygous flies have disruptions in the pattern of wing hair polarity. The prehair initiation site is along the cell periphery, but not restricted to the distal vertex in pupal wing cells. Double mutants of in¹ with fz^unspecified, dsh¹ or pk¹ resemble in¹ single mutants. Double mutants of in¹ with mwh¹ resemble mwh¹ single mutants. (Wong and Adler, 1993) Viable as a homozygote or a hemizygote. Strong thoracic bristle and wing hair phenotype. Percentage of dorsal wing surface cells that display abnormal cell polarity and the percentage of wing cells forming more than one hair is more severe at 18^oC than 29^oC. (Adler et al., 1994) Unmarked clones in all regions of the wing display an in phenotype, cells form more than one hair and hairs with an abnormal polarity. Marked clones in the wing indicate in acts autonomously, cells bordering clones are wild type. (Park et al., 1996) Mutants show no significant disruption of ovarian morphology. (Cohen et al., 2002) Hairs point slightly posteriorly in the C region of the wing. The hairs on the scutellum point anteriorly, and thorax microchaetae are routinely split. In the adult thorax some bristles are split. On the leg, hair polarity is abnormal, and more multiple leg hairs are found than in fz¹ mutants. Mispositioned bracts are not a feature of the phenotype, though bracts may be multiplied. Joint duplications are rare. (Lee and Adler, 2002)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference in¹ has tissue polarity defective \| somatic clone phenotype, non-enhanceable by grh^IM (Lee and Adler, 2004)
Suppressed by
Statement Reference in¹ has tissue polarity defective phenotype, suppressible by fz^Scer\UAS.cAa/Scer\GAL4^Dll-md23 (Lee and Adler, 2002)
NOT suppressed by
Statement Reference in¹ has tissue polarity defective \| somatic clone phenotype, non-suppressible by grh^IM (Lee and Adler, 2004)
NOT Enhancer of
Statement Reference in¹ is a non-enhancer of tissue polarity defective \| somatic clone phenotype of grh^IM (Lee and Adler, 2004)
Suppressor of
Statement Reference in¹ is a suppressor of tissue polarity defective phenotype of Scer\GAL4^Dll-md23, stan^Scer\UAS.cUa (Lee and Adler, 2002)
NOT Suppressor of
Statement Reference in¹ is a non-suppressor of tissue polarity defective \| somatic clone phenotype of grh^IM (Lee and Adler, 2004)
Phenotype Manifest In
Enhanced by
Statement Reference in¹ has wing hair \| somatic clone phenotype, enhanceable by ultA¹ (Adler et al., 2000) in¹ has wing hair & 1st posterior cell phenotype, enhanceable by mwh[+]/mwh¹ (Lee and Adler, 2002)
NOT Enhanced by
Statement Reference in¹ has wing hair \| somatic clone phenotype, non-enhanceable by grh^IM (Lee and Adler, 2004) in¹ has wing hair \| supernumerary \| somatic clone phenotype, non-enhanceable by grh^IM (Lee and Adler, 2004)
Suppressed by
Statement Reference in¹ has wing hair & 1st posterior cell phenotype, suppressible by fz^Scer\UAS.cAa/Scer\GAL4^Dll-md23 (Lee and Adler, 2002) in¹ has wing hair & 1st posterior cell phenotype, suppressible by Scer\GAL4^ptc-559.1/pk^Scer\UAS.cGa (Lee and Adler, 2002)
NOT suppressed by
Statement Reference in¹ has wing hair \| somatic clone phenotype, non-suppressible by grh^IM (Lee and Adler, 2004) in¹ has wing hair \| supernumerary \| somatic clone phenotype, non-suppressible by grh^IM (Lee and Adler, 2004) in¹ has microchaeta & adult thorax phenotype, non-suppressible by fz¹ (Lee and Adler, 2002) in¹ has phenotype, non-suppressible by su(Hw)² (Lindsley and Grell, 1968)
Enhancer of
Statement Reference in¹ is an enhancer of wing hair \| somatic clone phenotype of ultA¹ (Adler et al., 2000) in¹ is an enhancer of wing hair phenotype of sha^VB13 (Ren et al., 2006)
NOT Enhancer of
Statement Reference in¹ is a non-enhancer of wing hair \| somatic clone phenotype of grh^IM (Lee and Adler, 2004) in¹ is a non-enhancer of wing hair \| supernumerary \| somatic clone phenotype of grh^IM (Lee and Adler, 2004) in¹ is a non-enhancer of bract phenotype of fz¹ (Lee and Adler, 2002) in¹ is a non-enhancer of joint phenotype of fz¹ (Lee and Adler, 2002) in¹ is a non-enhancer of tarsal segment phenotype of fz¹ (Lee and Adler, 2002)
Suppressor of
Statement Reference in¹ is a suppressor of dorsal mesothoracic disc & pupa phenotype of fz¹ (Lee and Adler, 2002) in¹ is a suppressor of wing hair phenotype of Scer\GAL4^Dll-md23, stan^Scer\UAS.cUa (Lee and Adler, 2002)
NOT Suppressor of
Statement Reference in¹ is a non-suppressor of wing hair \| somatic clone phenotype of grh^IM (Lee and Adler, 2004) in¹ is a non-suppressor of wing hair \| supernumerary \| somatic clone phenotype of grh^IM (Lee and Adler, 2004) in¹ is a non-suppressor of bract phenotype of fz¹ (Lee and Adler, 2002) in¹ is a non-suppressor of eye phenotype of fz¹ (Lee and Adler, 2002) in¹ is a non-suppressor of joint phenotype of fz¹ (Lee and Adler, 2002) in¹ is a non-suppressor of ommatidium phenotype of fz¹ (Lee and Adler, 2002) in¹ is a non-suppressor of tarsal segment phenotype of fz¹ (Lee and Adler, 2002) in¹ is a non-suppressor of microchaeta & adult thorax \| somatic clone \| cell non-autonomous phenotype of fz¹ (Lee and Adler, 2002) in¹/in^II53 is a non-suppressor of wing hair \| conditional - heat sensitive phenotype of fz^hs.PP (Lee and Adler, 2002)
Additional Comments
Genetic Interactions
Statement Reference In somatic clones of cells that are homozygous for both in¹ and ultA¹, an increase is seen in the average number of wing hairs per cell. (Adler et al., 2000) Late gain of function phenotype of fz^hs.PP is not suppressible by in^II53/in¹ or fy⁶. Leg phenotypes of fz¹ and fy¹ or in¹ are additive. (Lee and Adler, 2002) When grh^IM mutant clones are made in an in¹ mutant wing the grh^IM phenotype is still seen. (Lee and Adler, 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	in^X2 (Lukinova et al., 1999) in^R3 (Lukinova et al., 1999) in^G5 (Lukinova et al., 1999)
Comments
Stocks ( 74 )
Kyoto	101651 in¹ 105927 cp¹ in¹ kni^ri-1 p^p 106005 st¹ in¹ kni^ri-1 p^p 107313 st¹ in¹ kni^ri-1 Scm^D1/TM3, Sb¹ 107137 Df(3L)31A/Dp(3;3)C126, cp¹ in¹ kni^ri-1 p^p 106012 th¹ st¹ cp¹ in¹ kni^ri-1 p^p 105945 fz¹ st¹ cp¹ in¹ kni^ri-1 p^p/TM1
Bloomington	607 st¹ in¹ kni^ri-1 p^p 1044 Crn¹ in¹ kni^ri-1/TM3, Sb¹ Ser¹ 2406 st¹ in¹ kni^ri-1 Scm^D1/TM3, Sb¹ 620 th¹ st¹ cp¹ in¹ kni^ri-1 p^p 505 fz¹ st¹ cp¹ in¹ kni^ri-1 p^p/TM1
	More stocks available...
Notes on Origin
Discoverer	Bridges, 20th Feb. 1926.

Comments
	Strong in allele. (Adler et al., 1994)
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	in¹ (Ren et al., 2006) unnamed (Roote, 2004.6.24)
Name Synonym
Secondary FlyBase IDs

References ( 15 )
Research paper	Ren et al., 2006, Genetics 172(3): 1643--1653 The shavenoid gene of Drosophila encodes a novel actin cytoskeleton interacting protein that promotes wing hair morphogenesis. [FBrf0190766] Lee and Adler, 2004, Mech. Dev. 121(1): 37--49 The grainy head transcription factor is essential for the function of the frizzled pathway in the Drosophila wing. [FBrf0167844] Cohen et al., 2002, Dev. Cell 2(4): 437--448 DWnt4 regulates cell movement and focal adhesion kinase during Drosophila ovarian morphogenesis. [FBrf0146974] Lee and Adler, 2002, Genetics 160(4): 1535--1547 The function of the frizzled pathway in the Drosophila wing is dependent on inturned and fuzzy. [FBrf0147064] Strutt, 2001, Molec. Cell 7(2): 367--375 Asymmetric localization of frizzled and the establishment of cell polarity in the Drosophila wing. [FBrf0134764] Adler et al., 2000, genesis 28(2): 82--91 Cell size and the morphogenesis of wing hairs in Drosophila. [FBrf0131239] Lukinova et al., 1999, Genetics 153(4): 1789--1797 Genetic characterization of cytological region 77A-D harboring the presenilin gene of Drosophila melanogaster. [FBrf0123112] Yun et al., 1999, Dev. Genet. 25(4): 297--305 The inturned protein of Drosophila melanogaster is a cytoplasmic protein located at the cell periphery in wing cells. [FBrf0112097] Park et al., 1996, Development 122(3): 961--969 The Drosophila tissue polarity gene inturned acts cell autonomously and encodes a novel protein. [FBrf0086571] Adler et al., 1994, Genetics 137(3): 829--836 The Drosophila tissue polarity gene inturned functions prior to wing hair morphogenesis in the regulation of hair polarity and number. [FBrf0072459] Wong and Adler, 1993, J. Cell Biol. 123(1): 209--221 Tissue polarity genes of Drosophila regulate the subcellular location for prehair initiation in pupal wing cells. [FBrf0064754]
Personal communication to FlyBase	Roote, 2004.6.24, Cam Dps. Cam Dps. [FBrf0178882] Roote, 2000.3.14, Dp(3;3)Cam's. Dp(3;3)Cam's. [FBrf0126882]
Book	Lindsley and Grell, 1968, Publs Carnegie Instn 627: 469pp Genetic variations of Drosophila melanogaster. [FBrf0020044] Bridges and Brehme, 1944, Publs Carnegie Instn 552: vii + 257pp The mutants of Drosophila melanogaster. [FBrf0006100]

Allele Dmel\in1

Allele Dmel\in¹