Allele Dmel\inaD^P215

General Information
Symbol	Dmel\inaD^P215	Species	D. melanogaster
Name		FlyBase ID	FBal0005607
Feature type	allele	Associated gene	Dmel\inaD

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:18,744,172..18,744,172 pr_change=M442K\|inaD-PA reported_pr_change=M442K comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T18744172A (Shieh and Niemeyer, 1995) point mutation 2R:18,744,172..18,744,172 na_change=T18744172A reported_na_change=T?A pr_change=M442K \| inaD-PA; M454K \| inaD-PB reported_pr_change=M442K (Shieh and Niemeyer, 1995)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Missense mutation in the third PDZ domain. (Tsunoda et al., 1997) Amino acid replacement: M442K. (Shieh and Zhu, 1995, Shieh and Zhu, 1996, Shieh and Niemeyer, 1995)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective (Gengs et al., 2002, Popescu et al., 2006) visual behavior defective (Leung et al., 2000) visual behavior defective \| dominant (Shieh and Niemeyer, 1995) visual behavior defective \| recessive (Shieh and Zhu, 1996)
Phenotype Manifest In
	photoreceptor cell (Chevesich et al., 1997, Leung et al., 2000) retina (Popescu et al., 2006)
Detailed Description
	Statement Reference In inaD¹ mutants, individual photoreceptors form the correct number of synapses per presynaptic terminal independently of cartridge composition. (Hiesinger et al., 2006) In red-eyed inaD[P215] homozygous flies flies dark-adapted for 2 minutes, the electro-retinogram (ERG) response to 2 second bright white-light stimulation displays the initial fast depolarization and maintenance during stimulation seen in wild-type. However, the recovery of polarisation following the end of stimulation is slower than in wild-type. This phenotype is not seen when low light levels are used. (Popescu et al., 2006) Mutants have defects in the on/off transients of the electroretinogram. Histamine-activated conductance in mutant laminar L1/L2 neurons appears indistinguishable from wild type. (Gengs et al., 2002) ERG responses are more wild type in peak amplitude, amount of decay during illumination, refractory period and response latency than those of trp². (Leung et al., 2000) The electroretinograms of young inaD¹ flies are abnormal; they show a delay in termination of the photoresponse. Exposure to a shorter light stimulus in these flies results in a less severe termination defect. (Li and Montell, 2000) inaC² inaD¹ double mutants show abnormal ERGs (electroretinograms) with response inactivation; they show a marked reduction in response to a second pulse of light. (Adamski et al., 1998) Light-dependent retinal degeneration. (Chevesich et al., 1997) norpA^C1094S.hs inaD¹ double mutants display a profound loss of the visual response. (Shieh et al., 1997) inaD¹ mutants have an electroretinogram (ERG) phenotype that approaches that of trp mutants. Flies show slow deactivation kinetics in response to a flash of light. The mean latency times between stimulus and quantum-bump generation are significantly greater than in wild-type flies, although quantum bumps from inaD¹ flies show normal termination kinetics. (Tsunoda et al., 1997) Double mutants of inaD¹ with trp² show the same ERG phenotype as trp² alone. (Shieh and Zhu, 1996) Patch clamp recordings of homo- and heterozygotes show a slow deactivation of the light-induced current, this defective deactivation is dependent of calcium influx. Photoreceptors show increased sensitivity to dim light. Two copies of P{hsp70-InsD} can completely rescue the wild type physiology of inaD¹ heterozygotes, two copies cannot rescue homozygotes. (Shieh and Niemeyer, 1995) Semi-dominant mutant of visual physiology, such that the phenotype (re PDA) of heterozygote similar to that produced by other ina homozygotes; inaD homozygotes exhibit ERG response that decays to baseline during bright light stimulus, and off transient is absent.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference inaD^P215, trpl³⁰² has visual behavior defective phenotype (Leung et al., 2000, Leung et al., 2000)
Phenotype Manifest In
NOT Suppressor of
Statement Reference inaD^P215 is a non-suppressor of rhabdomere phenotype of rdgA¹ (Georgiev et al., 2005)
Other
Statement Reference inaD^P215, trpl³⁰² has photoreceptor cell phenotype (Leung et al., 2000, Leung et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference Rhabdomeres of trpl³⁰²,inaD¹ double mutants are intact in 1 day old flies. ERG light responses of trpl³⁰²,inaD¹ and trpl³⁰²,trp² are small and transient, but the response amplitude of trpl³⁰²,inaD¹ is significantly smaller than that of trpl³⁰²,trp². Similarly, refractory period and response latency defects are more severe for trpl³⁰²,inaD¹ than for trpl³⁰²,trp². (Leung et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	42242 w^*; inaD^P215
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	inaD¹ (Cheng and Nash, 2007) inaD²¹⁵ (Tsunoda et al., 1997) inaD^P215 (Harteneck, 2003, Peng et al., 2008) InaD^P215 (Kumar and Shieh, 2001, Li and Montell, 2000, Leung et al., 2000, Liu et al., 2000, Montell, 1998, Ponting et al., 1997, Chevesich et al., 1997, Shieh and Zhu, 1996, Georgiev et al., 2005) inaD^p215 (Adamski et al., 1998, Shieh et al., 1997, Shieh et al., 1997, Shieh and Zhu, 1995, Shieh and Niemeyer, 1995, Hiesinger et al., 2006, Popescu et al., 2006) P215 (Gengs et al., 2002, )
Name Synonym
Secondary FlyBase IDs

References ( 22 )

Generate a list of	All references relating to this allele ( 22 )

List References by type	Research paper ( 16 ) Review ( 3 ) Abstract ( 2 ) FlyBase analysis ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...
Recent reviews (0)
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Allele Dmel\inaDP215

Allele Dmel\inaD^P215