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General Information
Symbol
Dmel\ix1
Species
D. melanogaster
Name
FlyBase ID
FBal0005628
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ix
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Point mutation.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ix1/ix2 mutants do not have surviving doublesex-expressing larval terminal neuroblasts in the terminal region of the female third instar larval ventral nerve cord, similar to controls.

Diplo XX ix1 homozygotes display variable degrees of intersexual characteristics, visible as incomplete and asymmetric development of the external terminalia and internal reproductive organs. The genital arch and anal plates are both affected, bristles are abnormal. Genital disc size is significantly reduced and it's morphology is abnormal. Cell death is elevated in homozygous mutant genital discs. In contrast, no major abnormalities are detected in the terminalia of XY ix1 homozygotes.

Homozygous XX ix1 flies are phenotypically intersexes, with simultaneous realisation of both male and female developmental pathways. The female and male genital primordia develop simultaneously in the genital discs of XX ix1/ix1 larvae.

The pheromone profile of ix1/Df(2R)en-B males is similar to that of wild-type males. The pheromone profile of ix1/Df(2R)en-B females is very different from that of wild-type females.

Diplo-X individuals carrying ix1 display an intersex phenotype. Within the range of intersexual phenotypes in homozygotes or hemizygotes both the male and female type morphological features tend to be more robust at lower temperatures and in hemizygotes. Heterozygotes with ix4 are intersexual at all temperatures, but the extent of intersexuality increases with increasing temperature.

Homozygotes transform XX;AA individuals into intersexes, the morphology of the internal and external genitalia is variable, bristles on the last transverse row of the basitarsus of the foreleg is morphologically intermediate and the genital duct system is duplicated with male and female systems. Homozygous males often have an enlarged sex comb area with the teeth arranged in more than one row. The males are sexually attractive but rarely court with active virgin females.

XX ix1/ix1, ix1/Df(2R)en-B and ix1/ix1; dsxD/+ flies do not show any male-specific courtship when paired with mature virgin females. 77% of XY ix1/ix1 flies, 70% of XY ix1/Df(2R)en-B and 50% of XY ix1/ix1; dsxD/+ flies show male-specific courtship when paired with mature virgin females.

Female flies lacking ix function have dorsal musculature appropriate to female flies, loss of ix function has no effect on the production of male specific muscle in males.

virts/virts ix1/+ XX flies are females (which are generally fertile) at 25oC.

Homozygous XX flies are intersex.

Females changed into sterile intersexes with a set of reduced male and a set of irregular female external genitalia. Gonads also mixed. They have no sex combs; pigmentation of abdomen intermediate between male and female. A large mass of chitinized tissue protrudes from vaginal opening. Homozygous males look normal but behave like intersexes (McRobert and Tompkins, 1985). They court young males as much as normal males do and occasionally court females; however, they are attractive to normal mature males. Heterozygous ix/+ males court females and young males, but seldom court mature males. The ix locus acts with the dsx female function to allow somatic differentiation in females (Nagoshi, McKeown, Burtis, Belote, and Baker, 1988).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

ix1 has genital disc phenotype, enhanceable by dppd-ho/dppd-ho

NOT Enhanced by
Statement
Reference

ix1 has terminalia phenotype, non-enhanceable by dsx11

ix1 has genital disc phenotype, non-enhanceable by wgl-17

NOT suppressed by
Statement
Reference

ix1 has genital disc phenotype, non-suppressible by wgl-17

Other
Additional Comments
Genetic Interactions
Statement
Reference

The phenotype of ix1 dsx11 female terminalia is almost comparable with ix1 single mutant terminalia.

A high frequency of tumour growth occurs below the anal plates of the terminalia in chromosomally female flies homozygous for ix1 and dppd-ho, dppd5 or dppd6, or homozygous for ix1 and trans-heterozygous for dppd5/dppd6, dppd5/dppd-ho, dppd6/dppd-ho or dppd5/dppd12. In contrast, no major abnormalities are detected in the terminalia of XY ix1 dppd-ho double homozygotes.

Homozygosity for dppd-ho significantly enhances the number of cell deaths in ix1 homozygous genital discs.

Males carrying dsxF.Hsp83 in an ix1/Df(2R)en-B background are phenotypically wild-type and fertile.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Germ line clonal analysis and pole cell transplantation experiments show that ix function is not required in the germ line for the normal development of germ cells according to their chromosomal sex.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (11)