General Information
Symbol
Dmel\kay1
Species
D. melanogaster
Name
FlyBase ID
FBal0005681
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
kay7P
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
T29791445A
Reported nucleotide change:
T1045A
Amino acid change:
L92term | kay-PA; L43term | kay-PB; L219term | kay-PD; L252term | kay-PF; L143term | kay-PG
Reported amino acid change:
L92term
Comment:
The presence of a nonsense mutation in kay1 is disputed by the authors of FBrf0100315 and FBrf0112258 who find that kay1 is caused by a deletion that breaks after the first exon and removes exon 1.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Df(3R)kay1 breaks between the first and second exons of kay and removes the first exon, extending to the upstream region of kay.
Nucleotide substitution: T1045A.
Amino acid replacement: L92term.
Deletion in the 5' region of kay, removing exon 1 (including the translation start site).
Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
kay1/kay1 clones (mushroom body) exhibit proliferation defects (low number of axons and lack of later-born neurons) but show normal axon pruning.
kay1 neuroblast clones in the mushroom body have no gross axonal phenotypes.
kay1 embryos show a complete failure of dorsal closure.
Heterozygous adults treated with hydrogen peroxide at the onset of metamorphosis do not show any defects.
Mutant embryos have a dorsal closure phenotype.
Homozygous embryos have a dorsal open phenotype. kay1/kay2 embryos have a dorsal open phenotype. kay1/kay2 adults rescued by the expression of kayhs.PZ have a marked cleft along the dorsal midline of the thorax.
Dorsal closure fails in homozygous embryos. The embryos have a large dorsal hole and the cuticle collapses. kay1/kay2 embryos have dorsal holes in the cuticle.
kay1/kay2 animals show a lethality and a thoracic cleft phenotype. These animals, do not recover when wounded. The epithelial cells at the edge of the wound also fail to undergo any evident cell shape change or show any cytoplasmic protusive extrusions. even after 18 hours after injury, the wound is not repaired.
When kayFbz.Scer\UAS is expressed under the control of Scer\GAL4dpp.blk1 in a kay1 heterozygote, there is an almost complete loss of longitudinal vein III. When kayFbz.Scer\UAS is expressed under the control of Scer\GAL4en-e16E in a kay1 heterozygote a more complete loss of longitudinal vein V is seen. When kayFbz.Scer\UAS is expressed under the control of Scer\GAL4sd-SG29.1 in a kay1 heterozygote all wing vein is strongly reduced.
Mosaic egg chambers in which the follicular epithelium is composed almost entirely of homozygous kay1 cells have a wild-type phenotype until stage 7/8 but degenerate during stage 9. This degeneration is associated with a failure of the main body follicle cells to accomplish their normal posteriorwards migration. Migration of border cells appears unaffected.
Embryos fail to show complete elongation of the dorsal epidermis after germband retraction. No bunching of the epidermis is seen.
Homozygous embryos exhibit a dorsal-open phenotype. kay1/kay2 transheterozygous embryos exhibit partially missing dorsal cuticle and the embryo is open dorsally.
Homozygotes die during embryogenesis, and have large anterior and dorsal holes, indicating a failure in dorsal closure and head involution. The leading edge epidermal cells initiate elongation transiently but subsequently resume the unelongated shape, while the more lateral epidermal cells elongate to a very minor extent and resume the typical polygonal shape after dorsal closure has been terminated prematurely. kay1/kay2 transheterozygotes are fully lethal as embryos at 25oC and 35% die as embryos at 18oC. The remaining 65% die as first instar larvae at 18oC. Homozygous embryos carrying two copies of kayαTub84B.PZ undergo normal dorsal closure and do not show the "dorsal open" phenotype, but fail to hatch. The degree of rescue is dependent on the dose of kayαTub84B.PZ. The lethality of kay1/kay2 transheterozygotes is rescued to adulthood by kayαTub84B.PZ. These adults have a cleft along the dorsal midline of the thorax. The lethality of kay1/kay2 transheterozygotes is also rescued to adulthood by kayhs.PZ. Some of these adults have a cleft along the dorsal midline of the thorax, and some appear wild-type.
Nondefective in gonad assembly.
embryonic lethal embryo open dorsally.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
kay1/kay2 has lethal phenotype, suppressible | partially by pucE69
kay1/kay2 has lethal phenotype, suppressible by pucE69/puc[+]
NOT suppressed by
Statement
Reference
kay1 has lethal | recessive phenotype, non-suppressible by Jra::kayJFJ.hs.sev
Enhancer of
Statement
Reference
kay1/kay[+] is an enhancer of partially lethal - majority die phenotype of slprBS06
Suppressor of
Statement
Reference
kay1/kay[+] is a suppressor of planar polarity defective phenotype of dshhs.sev.B
kay1/kay[+] is a suppressor of visible | dominant phenotype of hepCA
kay1/kay[+] is a suppressor of visible | dominant phenotype of sdSG29.1
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
kay1/kay2 has embryo | dorsal closure stage phenotype, non-enhanceable by eg18B
kay1/kay2 has embryo | dorsal closure stage phenotype, non-enhanceable by kni2
Suppressed by
NOT suppressed by
Statement
Reference
kay1/kay2 has embryo | dorsal closure stage phenotype, non-suppressible by eg18B
kay1/kay2 has embryo | dorsal closure stage phenotype, non-suppressible by kni2
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
kay1/kay[+] is a suppressor of lamellocyte | supernumerary phenotype of hopTum
kay1/kay[+] is a suppressor of ommatidium phenotype of dshhs.sev.B
kay1/kay[+] is a suppressor of wing phenotype of hepCA
kay1/kay[+] is a suppressor of ventral adult lateral neuron & axon phenotype of Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf
kay1 is a suppressor of wing | ectopic phenotype of EgfrE3
NOT Suppressor of
Statement
Reference
kay1/kay[+] is a non-suppressor of adult thorax phenotype of Mnn1UAS.cCa, Scer\GAL4pnr-MD237
Other
Additional Comments
Genetic Interactions
Statement
Reference
kay1/+ significantly suppresses the number of lamellocytes in the hemolymph of hopTum/+ larvae.
One copy of kay1 suppresses the formation of symmetrical ommatidia that is seen in animals expressing dshhs.sev.B.
A kay1/+ background has no effect on the Scer\GAL4pnr-MD237>Mnn1Scer\UAS.cCa phenotype.
The severity of the thoracic cleft seen in chm14 pharate adults is enhanced by heterozygosity for kay1.
mbf12 kay1/mbf12 double mutant adults treated with hydrogen peroxide at the onset of metamorphosis often have cuticle defects, which manifest as a depressed patch of naked cuticle at the dorsal midline.
The dorsal open phenotype of kay1 homozygous embryos is not rescued by Jra::kayJFJ.hs.sev. The dorsal open phenotype of kay1/kay2 and kay1 homozygous embryos is at least partially rescued by Jra::kayFJF.hs.sev. kay1/kay2 adults rescued by the expression of Jra::kayFJF.hs.sev have a marked cleft along the dorsal midline of the thorax.
The dorsal open phenotype caused by expression of egScer\UAS.cDa under the control of Scer\GAL4pnr-MD237 is enhanced by kay1. The dorsal closure phenotype caused by expression of knrlScer\UAS.cLa under the control of Scer\GAL4pnr-MD237 is enhanced by kay1. kay2/kay2 svp1 flies are viable, and sometimes have mild thorax closure defects.
The addition of pucE69 partially rescues the lethality and thoracic cleft phenotypes.
The lethality of the kay1/kay2 combination is rescued by one copy of pucE69; the rescued flies have a thoracic cleft phenotype ranging from strong to very mild.
Xenogenetic Interactions
Statement
Reference
Heterozygosity for kay1 suppresses the Scer\GAL4P2.4.Pdf>Hsap\APPScer\UAS.T:Hsap\MYC sLNv axonal arborization phenotype.
Complementation and Rescue Data
Partially rescued by
Comments
Embryonic phenotype is rescued by Scer\GAL469B-mediated expression of kayScer\UAS.cEa. Mutants are rescued to pharate adults, they exhibit a split thorax and frequently lack one wing. Scer\GAL4pnr-MD237-mediated expression of kayScer\UAS.cEa in kay1/kay2 transheterozygous embryos fails to rescue the dorsal open phenotype.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
Consists of a deletion that removes more than one complementation group.
Allelic series according to phenotypic strength: kay1 > kayED6315 > kayP54 > kayEY01644 > kay2.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (39)