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General Information
Symbol
Dmel\kay2
Species
D. melanogaster
Name
FlyBase ID
FBal0005682
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Mutants show a significant decrease in RP2 dendrite volume compared to controls.
Mutant embryos have a dorsal closure phenotype.
Homozygous embryos show a weak dorsal-open phenotype.
kay1/kay2 embryos have a dorsal open phenotype. Homozygous escapers have a marked cleft along the dorsal midline of the thorax. kay1/kay2 adults rescued by the expression of kayhs.PZ have a marked cleft along the dorsal midline of the thorax.
Homozygous embryos have a small dorsal anterior hole. kay1/kay2 embryos have dorsal holes in the cuticle.
23% of kay2 mutant pupae at 16 hours after puparium formation and 5% of pupae at 17-20 hours after puparium formation have persistent salivary glands.
kay1/kay2 animals show a lethality and a thoracic cleft phenotype.
Eggs derived from mosaic egg chambers in which the follicular epithelium is composed of homozygous kay2 cells consistently show two phenotypes; the eggs and their respiratory appendages are shorter than normal. In addition, two appendages of different lengths or "paddleless" appendages are often seen. Eggs which appear deflated are laid at a low frequency. The disappearance of nurse cell nuclei that normally occurs in stage 14 egg chambers is delayed in egg chambers in which the follicular epithelium is composed of homozygous kay2 cells, with many of the early stage 14 egg chambers containing as many as 15 remaining nurse cell nuclei.
Mutant embryos show a dorsal closure defect.
Thorax closure defects are seen in homozygotes at 6 hours after pupariation; the thoracic epithelium has not moved over the trachea and the three oblique muscles. At later stages, more severe defects are seen; the epithelium has retracted and fallen back into a folded state similar to that in third instar larvae. Homozygous adults have a cleft in the thorax at the dorsal midline, which causes a gap in the bristle pattern.
Homozygous embryos exhibit a partial dorsal-open phenotype.In a In(1)zae(bx) mutant background a fraction of kay2/Df(3R)01215 embryos die with mild dorsal-open phenotype (transvection is suppressed). kay1/kay2 transheterozygous embryos exhibit partially missing dorsal cuticle and the embryo is open dorsally.
50% of homozygotes die as embryos at 25oC, and 10% die as embryos at 18oC. Embryos may have dorsal holes. Approximately 1% of homozygotes can develop to adulthood, depending on the temperature and genetic background. These escapers occasionally have a cleft along the dorsal midline of the thorax. kay1/kay2 transheterozygotes are fully lethal as embryos at 25oC and 35% die as embryos at 18oC. The remaining 65% die as first instar larvae at 18oC. The lethality of kay1/kay2 transheterozygotes is rescued to adulthood by kayαTub84B.PZ. These adults have a cleft along the dorsal midline of the thorax. The lethality of kay1/kay2 transheterozygotes is also rescued to adulthood by kayhs.PZ. Some of these adults have a cleft along the dorsal midline of the thorax, and some appear wild-type.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
kay2 has lethal | recessive phenotype, suppressible by svp1
kay1/kay2 has lethal phenotype, suppressible | partially by pucE69
kay1/kay2 has lethal phenotype, suppressible by pucE69/puc[+]
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
kayhs.PZ/kay2 is a non-suppressor of visible | dominant phenotype of EgfrE3
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
kay1/kay2 has embryo | dorsal closure stage phenotype, non-enhanceable by eg18B
kay1/kay2 has embryo | dorsal closure stage phenotype, non-enhanceable by kni2
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
kay[+]/kay2 is an enhancer of adult thorax | pharate adult stage phenotype of chm14
Suppressor of
Statement
Reference
kay2 is a suppressor of wing vein | ectopic phenotype of rlSem
kay2 is a suppressor of eye phenotype of EgfrE3
kay2 is a suppressor of wing | ectopic phenotype of EgfrE3
kay2 is a suppressor of ommatidium phenotype of sevS11.Tag:MYC
NOT Suppressor of
Statement
Reference
kayhs.PZ/kay2 is a non-suppressor of wing vein | ectopic phenotype of rlSem
kayhs.PZ/kay2 is a non-suppressor of eye phenotype of EgfrE3
kayhs.PZ/kay2 is a non-suppressor of wing | ectopic phenotype of EgfrE3
kayhs.PZ/kay2 is a non-suppressor of ommatidium phenotype of sevS11.Tag:MYC
Other
Additional Comments
Genetic Interactions
Statement
Reference
Homozygous kay2 clones in the eye in a aope2d/+ background show ommatidial chirality, rotation and photoreceptor number defects (kay2 single mutant clones do not show tissue polarity defects by themselves).
The severity of the thoracic cleft seen in chm14 pharate adults is enhanced by heterozygosity for kay2.
The weak dorsal-open phenotype seen in homozygous kay2 embryos is enhanced by Cka05836/+.
The dorsal open phenotype of kay1/kay2 homozygous embryos is at least partially rescued by Jra::kayFJF.hs.sev. kay1/kay2 adults rescued by the expression of Jra::kayFJF.hs.sev have a marked cleft along the dorsal midline of the thorax.
kay2/kay2 svp1 flies are viable, and sometimes have mild thorax closure defects.
The addition of pucE69 partially rescues the lethality and thoracic cleft phenotypes. These animals, do not recover when wounded. The epithelial cells at the edge of the wound also fail to undergo any evident cell shape change or show any cytoplasmic protusive extrusions. even after 18 hours after injury, the wound is not repaired.
Mosaic egg chambers in which the follicle cells are homozygous for kay2 in a bsk1/+ background degenerate during stage 9 and show a complete failure of posteriorwards migration of the main body follicle cells (migration of the border cells appears unaffected).
The kay2 thoracic cleft phenotype is rescued by one copy of pucE69. The lethality of the kay1/kay2 combination is rescued by one copy of pucE69; the rescued flies have a thoracic cleft phenotype ranging from strong to very mild.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
Embryonic phenotype is rescued by Scer\GAL469B-mediated expression of kayScer\UAS.cEa. Mutants are rescued to pharate adults, they exhibit a split thorax and frequently lack one wing. Scer\GAL4pnr-MD237-mediated expression of kayScer\UAS.cEa in kay1/kay2 transheterozygous embryos fails to rescue the dorsal open phenotype.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments
Allelic series according to phenotypic strength: kay1 > kayED6315 > kayP54 > kayEY01644 > kay2.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (19)