Allele Dmel\kis¹

General Information
Symbol	Dmel\kis¹	Species	D. melanogaster
Name		FlyBase ID	FBal0005693
Feature type	allele	Associated gene	Dmel\kis

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Daubresse et al., 1999)
Mutagen	ethyl methanesulfonate (Kennison and Tamkun, 1988)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Kennison and Tamkun, 1988) visible \| homeotic \| recessive \| somatic clone (Daubresse et al., 1999) visible \| recessive \| somatic clone (Terriente-Félix et al., 2011) visible \| somatic clone (Arancio et al., 2010)
Phenotype Manifest In
	1st posterior cell \| somatic clone (Terriente-Félix et al., 2011) adult abdominal segment 5 \| cell autonomous \| somatic clone (Daubresse et al., 1999) cephalopharyngeal skeleton \| germline clone (Daubresse et al., 1999) denticle belt \| germline clone (Daubresse et al., 1999) embryonic abdominal segment 2 \| germline clone (Daubresse et al., 1999) embryonic abdominal segment 4 \| germline clone (Daubresse et al., 1999) embryonic abdominal segment 6 \| germline clone (Daubresse et al., 1999) embryonic abdominal segment 8 \| germline clone (Daubresse et al., 1999) embryonic head \| germline clone (Daubresse et al., 1999) embryonic mesothoracic segment \| germline clone (Daubresse et al., 1999) embryonic metathoracic segment \| germline clone (Daubresse et al., 1999) eye \| somatic clone (Arancio et al., 2010) larval mesothoracic sensillum \| embryonic stage \| germline clone (Daubresse et al., 1999) larval metathoracic sensillum \| embryonic stage \| germline clone (Daubresse et al., 1999) mesothoracic ventral denticle belt \| germline clone (Daubresse et al., 1999) metathoracic femur \| somatic clone (Daubresse et al., 1999) metathoracic Keilin's organ \| germline clone (Daubresse et al., 1999) metathoracic leg \| somatic clone (Daubresse et al., 1999) metathoracic metatarsus \| somatic clone (Daubresse et al., 1999) metathoracic tarsal segment 2 \| somatic clone (Daubresse et al., 1999) metathoracic tarsal segment 3 \| somatic clone (Daubresse et al., 1999) metathoracic tarsal segment 4 \| somatic clone (Daubresse et al., 1999) metathoracic tarsal segment 5 \| somatic clone (Daubresse et al., 1999) metathoracic tibia \| somatic clone (Daubresse et al., 1999) metathoracic ventral denticle belt \| germline clone (Daubresse et al., 1999) wing \| somatic clone (Terriente-Félix et al., 2011) wing vein \| ectopic \| somatic clone (Terriente-Félix et al., 2011)
Detailed Description
	Statement Reference Clones of kis[1] mutant cells induced between wing veins L2 and L5 engender ectopic vein formation close to the position of longitudinal veins in both the anterior and posterior compartments and broadening of the L3-L4 intervein region. When mutant kis[1] clones occupy the entire wing, this results in a reduction in wing size, loss of the L2 vein and increase in the distance between the L3 and L4 veins. Somatic clones of kis[1] mutant cells in the posterior compartment engender the formation of ectopic wing veins of normal thickness and dorso-ventral characteristics. kis[1] mutant clones located adjacent or in longitudinal wing vein 3 (L3) cause the duplication of this vein. kis[1] mutant cells contribute to L3 when the size of the clone is very small, but in most cases kis[1] mutant cells differentiate as intervein, and the ectopic L3 vein is composed of wild-type cells surrounding the mutant clone. kis[1] mutant clones located between longitudinal wing veins L3 and L4 cause a variable increase in the L3-L4 intervein. This increase is proportional to the fraction of kis[1] mutant cells occupying the L3-L4 intervein. (Terriente-Félix et al., 2011) Homozygous clones in the fifth adult abdominal segment show transformation to a more anterior identity, as shown by the loss of the black pigmentation characteristic of this segment. The effect is cell autonomous. The average size and frequency of homozygous clones induced at the cellular blastoderm stage are not significantly reduced in any body segments relative to control clones. Clones in the second leg have no phenotypic abnormalities. Clones in the third leg show abnormalities, particularly in the distal leg structures; the femur and tibia are slightly abnormal, while the tarsal segments are severely distorted. All five tarsal segments are present but are truncated and have a hooked shape. Homozygous embryos derived from homozygous female germline clones (lacking both maternal and zygotic kis function) show a deletion of pattern elements approximating alternate segments. The most common defect is a reduction in size of one or more alternate segments (T3, A2, A4, A6 and sometimes A8). In more extreme cases only one or two patches of denticle belt are seen. The second thoracic segment is often missing or severely distorted and the head region is grossly abnormal. The cephalopharyngeal skeleton is internalised and malformed (possibly as a consequence of the major pattern alterations). The loss of the T2 and T3 denticle belts is accompanied by loss of their associated sensory organs (both the Keilin's organ and campaniform sensilla). The first thoracic segment does not appear to be deleted or transformed. (Daubresse et al., 1999) brm² shows little if any interaction in double heterozygous combination with kis¹; less than 3% of flies have held-out wings. (Vazquez et al., 1999) Mutation does not affect the level of w expression in ph-p^lac+3 flies. (Fauvarque et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference kis¹ is an enhancer of visible \| dominant phenotype of tna¹ (Gutierrez et al., 2003)
NOT Enhancer of
Statement Reference kis¹ is a non-enhancer of visible phenotype of BEAF-32^Scer\UAS.cYa, Scer\GAL4^GMR.PS (Yamaguchi et al., 2001) kis¹ is a non-enhancer of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.Scer\UAS.T:Ivir\HA1} (Armstrong et al., 2005)
Suppressor of
Statement Reference kis¹ is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^{S11.T:Hsap\MYC} (Maixner et al., 1998)
NOT Suppressor of
Statement Reference kis¹ is a non-suppressor of visible phenotype of BEAF-32^Scer\UAS.cYa, Scer\GAL4^GMR.PS (Yamaguchi et al., 2001) kis¹ is a non-suppressor of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.Scer\UAS.T:Ivir\HA1} (Armstrong et al., 2005) kis¹ is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dm^Scer\UAS.cZa (Secombe et al., 2007)
Other
Statement Reference Ras85D^V12.sev, kis¹, sev^{S11.T:Hsap\MYC} has viable phenotype (Maixner et al., 1998, Maixner et al., 1998)
Phenotype Manifest In
Enhancer of
Statement Reference kis[+]/kis¹ is an enhancer of eye \| heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰ (Melicharek et al., 2008) kis[+]/kis¹ is an enhancer of photoreceptor cell \| heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰ (Melicharek et al., 2008) kis[+]/kis¹ is an enhancer of wing vein \| ectopic phenotype of Snr1^E1 (Marenda et al., 2004) kis¹ is an enhancer of wing phenotype of tna¹ (Gutierrez et al., 2003)
NOT Enhancer of
Statement Reference kis¹ is a non-enhancer of eye phenotype of BEAF-32^Scer\UAS.cYa, Scer\GAL4^GMR.PS (Yamaguchi et al., 2001) kis¹ is a non-enhancer of eye phenotype of CycE^JP (Brumby et al., 2002) kis¹ is a non-enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.Scer\UAS.T:Ivir\HA1} (Armstrong et al., 2005)
Suppressor of
Statement Reference kis¹ is a suppressor of phenotype of Pc⁴ (Kennison and Tamkun, 1988)
NOT Suppressor of
Statement Reference kis¹ is a non-suppressor of eye phenotype of BEAF-32^Scer\UAS.cYa, Scer\GAL4^GMR.PS (Yamaguchi et al., 2001) kis¹ is a non-suppressor of eye phenotype of CycE^JP (Brumby et al., 2002) kis¹ is a non-suppressor of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.Scer\UAS.T:Ivir\HA1} (Armstrong et al., 2005) kis¹ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dm^Scer\UAS.cZa (Secombe et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference A kis[1] heterozygous background gives a dominant mild enhancement to the ato[1090]/Df(3R)p13 eye phenotype at 25[o]C. (Melicharek et al., 2008) Has little or no effect on the phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH flies. (Staehling-Hampton et al., 1999) The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by kis¹. (Maixner et al., 1998) Suppresses the extra sex comb phenotype of Pc⁴, Ts(YLt;2Lt)L124. Causes between 50% and 100% suppression of the Pc⁴/+ extra sex combs phenotype. (Kennison and Tamkun, 1988)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	kis^AO9 (Melicharek et al., 2008) kis^AS21 (Melicharek et al., 2008) kis^EC1 (Melicharek et al., 2008) kis^LM27 (Melicharek et al., 2008)
Comments
Stocks ( 2 )
Bloomington	431 kis¹ cn¹ bw¹ sp¹/SM6a
Kyoto	105909 kis¹ cn¹ bw¹ sp¹/SM6a
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	kis¹ (Arancio et al., 2010, Vázquez et al., 2008, Bhatia et al., 2010, Terriente-Félix et al., 2011, Melicharek et al., 2008) Kis¹ (Secombe et al., 2007) kiss¹ (Bejarano and Busturia, 2004)
Name Synonym
Secondary FlyBase IDs

References ( 24 )

Generate a list of	All references relating to this allele ( 24 )

List References by type	Research paper ( 22 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 1 )
	Terriente-Félix et al., 2011, Dev. Biol. 350(2): 382--392 A conserved function of the chromatin ATPase Kismet in the regulation of hedgehog expression. [FBrf0212899] Show all research papers ...

Allele Dmel\kis1

Allele Dmel\kis¹