FB2025_01 , released February 20, 2025
Allele: Dmel\cac20-3
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General Information
Symbol
Dmel\cac20-3
Species
D. melanogaster
Name
FlyBase ID
FBal0006145
Feature type
allele
Associated gene
Dmel\cac
Associated Insertion(s)
Carried in Construct
Also Known As
l(1)L1320-3
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Kulkarni and Hall, 1987)
Progenitor genotype
Cytology
Description

cac20-3 contains a frame-shifting 341bp deletion in the third homology domain.

(Kurshan et al., 2009)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      cac20-3 mutant embryos exhibit normal amplitude mEJPs but severely reduced synaptic transmission. No evoked response can be detected in 1mM Ca[2+] Saline, compared to an average response of over 1nA in wild-type embryos.

      cac20-3 mutant embryos develop boutons and exhibit normal Ank2 localisation.

      (Kurshan et al., 2009)

      cac20-3/cacNT27 flies are flightless, have an uncoordinated gait and have a reduced viability and life span. cac20-3/cacNT27 third instar larvae show a dramatic defect in evoked neurotransmitter release at the neuromuscular junction compared to controls at 22oC. At low extracellular calcium levels, evoked release is undetectable in the mutant larvae, whereas control larvae show substantial synaptic transmission. At higher extracellular calcium levels, evoked release is partially restored in the mutant larvae, but the quantal content is greatly reduced compared to controls. The amplitude of the miniature excitatory junctional potential is unchanged in the mutant larvae compared to controls. cac20-3/cacNT27 third instar larvae show a significant reduction in the number of varicosities at the neuromuscular junction of muscle fibres 6 and 7 compared to wild-type larvae (varicosity number per muscle surface area is also reduced). cac20-3/cacNT27 third instar larvae also show a significant reduction in the number of varicosities at the neuromuscular junction of muscle fibre 4. cac20-3/Df(1)RC29 embryos show no defect in the number of varicosities or innervation pattern at the neuromuscular junction of muscle fibres 6 and 7.

      (Rieckhof et al., 2003)

      Song pulse amplitude in heterozygotes with cac1 is higher than wild type.

      (Kulkarni and Hall, 1987)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      cac20-3 is a non-enhancer of abnormal neurophysiology | embryonic stage phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-enhancer of lethal | late embryonic stage phenotype of stjDD106

      (Kurshan et al., 2009)
      Suppressor of
      Statement
      Reference

      cac20-3 is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of stjDD196

      (Kurshan et al., 2009)
      NOT Suppressor of
      Statement
      Reference

      cac20-3 is a non-suppressor of abnormal neurophysiology | embryonic stage phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-suppressor of lethal | late embryonic stage phenotype of stjDD106

      (Kurshan et al., 2009)
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      cac20-3 is a non-enhancer of embryonic/larval neuromuscular junction phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-enhancer of motor neuron phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-enhancer of bouton phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-enhancer of synapse phenotype of stjDD106

      (Kurshan et al., 2009)
      Suppressor of
      Statement
      Reference

      cac20-3 is a suppressor of bouton phenotype of stjDD196

      (Kurshan et al., 2009)
      NOT Suppressor of
      Statement
      Reference

      cac20-3 is a non-suppressor of embryonic/larval neuromuscular junction phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-suppressor of motor neuron phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-suppressor of bouton phenotype of stjDD106

      (Kurshan et al., 2009)

      cac20-3 is a non-suppressor of synapse phenotype of stjDD106

      (Kurshan et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      cac20-3; stjDD106 double mutants are indistinguishable from stjDD106 single mutants.

      cac20-3; stjDD196 double mutants are able to form boutons.

      (Kurshan et al., 2009)

      Ca-α1DX10 cac20-3/Df(1)RC29 double mutant embryos show no defect in the number of varicosities or innervation pattern at the neuromuscular junction of muscle fibres 6 and 7.

      (Rieckhof et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      cac1

      (Kulkarni and Hall, 1987)
      Rescued by

      cac20-3 is rescued by Scer\GAL4elav-C155/cacUAS.EGFP

      (Kawasaki et al., 2004)
      Partially rescued by

      cac20-3 is partially rescued by Scer\GAL4elav-C155/cacUAS.cKa

      (Kawasaki et al., 2002)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Lefevre.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      Dmca1A13
      (Rieckhof et al., 2003)
      L(1)L13
      (Rieckhof et al., 2003)
      cac20-3
      cacL1320-3
      (Kurshan et al., 2009)
      l(1)11Aa13
      l(1)L1320-3
      (Kawasaki et al., 2004, Kawasaki et al., 2002, Kulkarni and Hall, 1987)
      nbA20-3
      Name Synonyms
      Secondary FlyBase IDs
        References (5)