Allele Dmel\CrebB^S162

General Information
Symbol	Dmel\CrebB^S162	Species	D. melanogaster
Name		FlyBase ID	FBal0006239
Feature type	allele	Associated gene	Dmel\CrebB
Also Known As	S162, DCREB^S162, CrebB^S162, CrebB-17A^S162

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Genes CrebB
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Eberl et al., 1992)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Iijima-Ando et al., 2005) lethal \| larval stage \| recessive (Eberl et al., 1992, Yin, 1997) lethal \| recessive (Belvin et al., 1999) locomotor rhythm defective \| recessive (Belvin et al., 1999) neuroanatomy defective \| third instar larval stage (Chen and Ganetzky, 2012) viable \| recessive (Iijima-Ando et al., 2005)
Phenotype Manifest In
	adult (Belvin et al., 1999) NMJ bouton \| third instar larval stage (Chen and Ganetzky, 2012)
Detailed Description
	Statement Reference There is a significant decrease in synaptic bouton numbers at larval neuromuscular junctions (NMJs), compared with wild-type, in CrebB-17A[S162] mutants. Heterozygous CrebB-17A[S162] larvae have normal NMJs. (Chen and Ganetzky, 2012) Homozygous CrebB-17A[S162] mutants are lethal. A single copy of CrebB-17A[S162] does not cause any lethality. (Iijima-Ando et al., 2005) CrebB-17A^S162, though lethal, is not completely penetrant, less than 0.5% of flies survive to adulthood. These escapers are about three quarters the size of wild type flies, but otherwise normal in appearance. Of 34 mutant flies tested for circadian locomotor activity, 13 (38%) were arrhythmic and the rest showed a short period averaging 22.8 hours. (Belvin et al., 1999) Germline clones demonstrate no maternal effect. Zygotic lethality acts in the third larval instar stage. (Eberl et al., 1992)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference CrebB^S162, Hsap\HTT^{Q48.ex1p.Scer\UAS}, Scer\GAL4^elav-C155 has lethal phenotype, suppressible by CrebB^+t.STOP (Iijima-Ando et al., 2005)
Enhancer of
Statement Reference CrebB^S162/CrebB-17A[+] is an enhancer of partially lethal - majority die phenotype of CrebB^+tIa/CrebB-17A[+], Hsap\HTT^{Q48.ex1p.Scer\UAS}, Scer\GAL4^elav-C155 (Iijima-Ando et al., 2005) CrebB^S162/CrebB-17A[+] is an enhancer of partially lethal - majority die phenotype of Hsap\HTT^{Q48.ex1p.Scer\UAS}, Scer\GAL4^elav-C155 (Iijima-Ando et al., 2005)
Suppressor of
Statement Reference CrebB^+tIa, CrebB^S162, CrebB-17A[+], CrebB-17A[+] is a suppressor of partially lethal - majority die phenotype of CrebB-17A[+]:, Hsap\HTT^{Q48.ex1p.Scer\UAS}, Scer\GAL4^elav-C155 (Iijima-Ando et al., 2005)
NOT Suppressor of
Statement Reference CrebB^S162/CrebB-17A[+] is a non-suppressor of viable phenotype of Hsap\HTT^{Q22.ex1p.Scer\UAS}, Scer\GAL4^elav-C155 (Iijima-Ando et al., 2005)
Other
Statement Reference CrebB^S162, Df(1)Exel9051, Df(1)Exel9051, + has neuroanatomy defective \| third instar larval stage phenotype (Chen and Ganetzky, 2012) CrebB^S162, Hsap\HTT^{Q48.ex1p.Scer\UAS}, Scer\GAL4^elav-C155 has lethal phenotype (Iijima-Ando et al., 2005)
Phenotype Manifest In
NOT Enhancer of
Statement Reference CrebB^S162, Scer\GAL4^30A is a non-enhancer of wing phenotype of Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A (Kiger et al., 2001)
Suppressor of
Statement Reference CrebB^S162 is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Crtc^Scer\UAS.cWa (Wang et al., 2008)
NOT Suppressor of
Statement Reference CrebB^S162, Scer\GAL4^30A is a non-suppressor of wing phenotype of Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A (Kiger et al., 2001)
Other
Statement Reference CrebB^S162, Df(1)Exel9051, Df(1)Exel9051, + has embryonic/larval neuromuscular junction \| third instar larval stage phenotype (Chen and Ganetzky, 2012)
Additional Comments
Genetic Interactions
Statement Reference Heterozygosity for both CrebB-17A[S162] and Df(1)Exel9051 results in a significant reduction in neuromuscular junction (NMJ) growth. (Chen and Ganetzky, 2012) A CrebB-17A[S162] background suppresses the rough eye phenotype found in TORC[Scer\UAS.cWa]-expressing (under the control of Scer\GAL4[GMR.PF]) flies. (Wang et al., 2008)
Xenogenetic Interactions
Statement Reference The survival rate of flies expressing Hsap\HD[Q48.ex1p.Scer\UAS] in the nervous system (under the regulation of Scer\GAL4[elav-C155]) is sensitive to the gene dosage of CrebB-17A[S162] flies. A CrebB-17A[S162] heterozygous background more than doubles the lethality of Hsap\HD[Q48.ex1p.Scer\UAS] flies compared to flies expressing Hsap\HD[Q48.ex1p.Scer\UAS] alone (under the control of Scer\GAL4[elav-C155]). Addition of a heterozygous CrebB-17A[S162] background to CrebB-17A[+tIa] Hsap\HD[Q48.ex1p.Scer\UAS] (Scer\GAL4[elav-C155]) flies reduces the survival rate slightly, although lethality is not as high as in Hsap\HD[Q48.ex1p.Scer\UAS] (Scer\GAL4[elav-C155]) flies. The survival rate of flies expressing Hsap\HD[Q22.ex1p.Scer\UAS] in the nervous system (under the regulation of Scer\GAL4[elav-C155]) is not sensitive to the gene dosage of CrebB-17A[S162] flies. Homozygous CrebB-17A[S162] flies expressing Hsap\HD[Q48.ex1p.Scer\UAS] under the control of Scer\GAL4[elav-C155] are lethal. Addition of the CrebB-17A[+t.STOP] transgene to homozygous CrebB-17A[S162] flies expressing Hsap\HD[Q48.ex1p.Scer\UAS] under the control of Scer\GAL4[elav-C155] results in an approximate 1% survival rate. (Iijima-Ando et al., 2005)
Complementation & Rescue Data
Rescued by	CrebB^S162 is rescued by CrebB^+tIa (Iijima-Ando et al., 2005) CrebB^S162 is rescued by CrebB^d.hs (Belvin et al., 1999)
Not rescued by	CrebB^S162 is not rescued by CrebB^+t.STOP (Iijima-Ando et al., 2005)
Comments	Lethality can be almost fully rescued by an induced transgene carrying CrebB-17A, partial rescue is achieved with the non-induced transgene. Interpretation of rescue experiments is complicated as background genetic variability also gives partial rescue. (Yin, 1997)
Stocks ( 2 )
Bloomington	4720 y¹ CrebB^S162/FM6
Kyoto	107845 y¹ CrebB^S162/FM6
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	CREB^S162 (Iijima-Ando et al., 2009) creb B-17A^S162 (Kiger et al., 2001) CrebB-17A^S162 (Popodi et al., 2010-, Kang et al., 2011) CrebB^S162 (Fox et al., 2010, Wang et al., 2008, ) dCreb2^S162 (Chen and Ganetzky, 2012) DCREB^S162 (Stanewsky, 2003) dCREB^S162 (Iijima-Ando et al., 2009) l(1)17Af¹ l(1)S162 S162 (Belvin et al., 1999, Eberl et al., 1992, Iijima-Ando et al., 2005)
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543 A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500] Kang et al., 2011, PLoS ONE 6(12): e29800 Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila. [FBrf0217065] Fox et al., 2010, J. Cell Biol. 191(3): 479--492 The CrebA/Creb3-like transcription factors are major and direct regulators of secretory capacity. [FBrf0212217] Iijima-Ando et al., 2009, PLoS ONE 4(12): e8310 Mitochondrial mislocalization underlies Abeta42-induced neuronal dysfunction in a Drosophila model of Alzheimer's disease. [FBrf0209556] Wang et al., 2008, Cell Metab. 7(5): 434--444 The insulin-regulated CREB coactivator TORC promotes stress resistance in Drosophila. [FBrf0204686] Iijima-Ando et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(29): 10261--10266 cAMP-response element-binding protein and heat-shock protein 70 additively suppress polyglutamine-mediated toxicity in Drosophila. [FBrf0188259] Kiger et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(18): 10190--10195 Hemocytes are essential for wing maturation in Drosophila melanogaster. [FBrf0138518] Belvin et al., 1999, Neuron 22(4): 777--787 The Drosophila dCREB2 gene affects the circadian clock. [FBrf0108141] Eberl et al., 1992, Genetics 130: 569--583 Genetic and developmental analysis of polytene section 17 of the X chromosome of Drosophila melanogaster. [FBrf0056238]
Review	Stanewsky, 2003, J. Neurobiol. 54(1): 111--147 Genetic analysis of the circadian system in Drosophila melanogaster and mammals. [FBrf0155881]
Personal communication to FlyBase	Popodi et al., 2010-, Small X duplications for the stock center collection. Small X duplications for the stock center collection. [FBrf0210621]
Abstract	Yin, 1997, A. Rep. Cold Spring Harbor Lab. 1996: 174--177 Long-term memory formation in Drosophila. [FBrf0098128]

Allele Dmel\CrebBS162

Allele Dmel\CrebB^S162