FlyBase Allele Report: Dmel\lola[00642]

General Information

Symbol

Dmel\lola⁰⁰⁶⁴²

Species

D. melanogaster

Name

FlyBase ID

FBal0007954

Feature type

allele

Associated gene

Dmel\lola

Associated Insertion(s)

P{PZ}lola⁰⁰⁶⁴²

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}lola⁰⁰⁶⁴²

Cytology

Description

P{PZ} insertion 438bp downstream of 5'UTR "c" and 54bp upstream of 5'UTR "d" of lola.

(Zhang et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:10,534,412..10,534,412 [+]

(Otte et al., 2009, Bass et al., 2007, Zhang et al., 2003, Spradling et al., 1999, Braun et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Bass et al., 2007)

lethal (with Df(2R)12)

(Strub et al., 2008)

lethal (with Df(2R)E3363)

(Strub et al., 2008)

lethal (with Df(2R)ED2098)

(Kahsai et al., 2016)

lethal (with lola⁶²⁹)

(Bass et al., 2007)

lethal (with lola^k09901)

(Kahsai et al., 2016)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

egg chamber | germline clone

(Bass et al., 2007)

larval somatic muscle cell

(Dobi et al., 2014)

larval somatic muscle cell (with Df(2R)ED2076)

(Dobi et al., 2014)

nurse cell | germline clone

(Bass et al., 2007)

Detailed Description

Statement

Reference

Females containing homozygous germline clones show defects in stage 14 egg chambers, including dumpless egg chambers (egg chambers in which the nurse cells have failed to transfer their cytoplasmic contents to the oocyte, have intact nuclei and do not appear to be undergoing programmed cell death) and egg chambers with persisting nurse cell nuclei (egg chambers in which nurse cells have transferred their cytoplasmic contents to the oocyte, but their nuclei have failed to undergo programmed cell death including nuclear condensation).

Mutants have a strong mutant neuronal phenotype.

(Bass et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

lola[+]/lola⁰⁰⁶⁴² is a suppressor of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

lola[+]/lola⁰⁰⁶⁴² is a suppressor of lethal phenotype of JIL-1^EP3657

(Zhang et al., 2003)

Other

Statement

Reference

SoxN^GA1192, lola[+]/lola⁰⁰⁶⁴² has partially lethal phenotype

(Girard et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

lola[+]/lola⁰⁰⁶⁴² is a suppressor of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

Other

Statement

Reference

SoxN^GA1192, lola[+]/lola⁰⁰⁶⁴² has larval longitudinal connective phenotype

(Girard et al., 2006)

SoxN^GA1192, lola[+]/lola⁰⁰⁶⁴² has axon phenotype

(Girard et al., 2006)

SoxN^GA1192, lola[+]/lola⁰⁰⁶⁴² has larval anterior commissure phenotype

(Girard et al., 2006)

SoxN^GA1192, lola[+]/lola⁰⁰⁶⁴² has larval posterior commissure phenotype

(Girard et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

lola⁰⁰⁶⁴²/+, SoxN^GA1192/+ double heterozygous embryos show defects in axon scaffolding, such as fusion of commissures, axonal tangles, disruption and/or reduction of longitudinal connectives. This phenotype is not seen in either single mutant heterozygote.

(Girard et al., 2006)

The hatch rate of lola⁰⁰⁶⁴²/+ ; JIL-1^EP3657/JIL-1^EP3657 embryos is 20%.

(Zhang et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

ex^k05915a