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General Information
Symbol
Dmel\tutl01085
Species
D. melanogaster
Name
FlyBase ID
FBal0007959
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)01085
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} insertion within exon 4, disrupting translation downstream of exon 4.

P{PZ} insertion in exon 6 of the CT35488 transcript of tutl, 20251bp downstream of the 5' end of the transcript.

Insertion components
P{PZ}tutl01085
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

tutl23/tutl01085 and tutl01085/Df(2L)tutl larvae show a severe reduction compared to wild-type controls in changing moving direction in response to gentle touch at anterior segments. The number of exploratory head swings in response to the touch, and the time taken to select a new moving direction are increased. The withdrawal response to the gentle touch is normal.

Locomotory behavior in a stimulus-free condition is normal in tutl23/tutl01085 larvae (number of contractions, average speed, number of turns and average turning angles have been analysed).

tutl23/tutl01085 larvae show normal phototactic behaviour.

In tutl01085 embryos, the ISNb motor axons succesfully reach the vicinity of their respective targets. However, once there, many fail to send one or more of the final axon branches to contact their muscle targets. Around a quarter of the hemisegments also lack ISNd nerves.

Embryos trans-heterozygous for tutlex383 and tutl01085 have defects in commissures, longitudinal connective, and Fas2-positive axon tracts that maintain their relative distance from the midline, but that have axons emanating from all three fascicles bundle together as they cross the midline.

In tutl01085 eye-specific mosaic animals in which over 90% of R7 and R8 cells are homozygous for tutl01085, the R7 and R8 axons are disorganised, despite R7 and R8 terminal layers being evident. Many axonal terminals display abnormal lateral extensions and frequently fuse together, particularly at the R7 terminal layer. tutl01085 mutant R7 and R8 axons from the same ommatidium still associate with each other within the same column.

Single tutl01085 mutant R7 axonal somatic clones display abnormal lateral extension. Compared to wild-type, the frequency of fusion between a labelled tutl01085 mutant R7 axonal terminal and it's neighbors is increased by approximately 4-10 fold. Among them approximately 53% display a 'U-shape' tiling phenotype, in which a mutant R7 terminal branches out from its column at the R7 recipient layer, extend laterally and fuses with its neighboring R7 terminal. In addition, 47% of terminal fusions display a 'V-shape' phenotype in which a mutant R7 terminal appears to move away from its own column and fuses with its neighboring R7 terminal at the R7 recipient layer. Neighboring wild-type R7 terminals also display a similar degree of tiling defects. Many of them display abnormal lateral extension and frequently extend and fuse with their neighboring R7 terminals, indicating a cell non-autonomous tiling role for tutl between R7 terminals. The majority of defective wild-type R7 axons extend towards their neighboring tutl01085 mutant axons.

In the absence of neighboring R7 terminals, the tendency of a tutl01085 mutant axon to invade a neighboring column is decreased from approximately 33% for surrounded R7 terminals to approximately 15% for isolated R7 terminals.

Class I ddaE neurons in tutl01085/tutl23 larvae have defects in their dendritic trees, including shortened interstitial branches and curled growth lacking directed orientation. There are also significantly more branch termini per neuron compared to wild type. The number of branch points on primary dendrites is unchanged compared to controls, but there is a clear increase in second and third order branch points in the mutant neurons.

tutl01085/Df(2L)ed-dp larvae show defects in the dendritic trees of class I ddaE neurons; there are significantly more branch termini per neuron compared to wild type, and although the number of branch points on primary dendrites is unchanged compared to controls, there is a clear increase in second and third order branch points in the mutant neurons.

Class IV ddaC neurons in tutl01085/tutl23 larvae show numerous dendrite crossing points, in contrast to control neurons.

tutl01085/tutl23 larvae show normal dendritic tiling among class IV da neurons (assayed by examining the borders between ddaC and v'ada neurons for dendritic overlap).

Lethal stage is during mid-eclosion. General morphology of the mutant larvae is normal. Mutant larvae respond to tactile stimulation at their anterior end by contracting at both ends and rocking back and forth in place. After a few seconds of this behaviour, the larvae cease this abnormal movement and return to a normal forward-crawling motion. This contrasts with wild-type larvae which respond by showing a characteristic escape behaviour of 1-3 reverse peristaltic movements followed by a lateral turning behaviour. tutl01085/Df(2L)tutl4 larvae have a severely compromised ability to roll over from an inverted position; the time required to right themselves is significantly longer than control larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

tutl01085/tutl[+] is a non-enhancer of visible | heat sensitive phenotype of peb1

NOT Suppressor of
Statement
Reference

tutl01085/tutl[+] is a non-suppressor of visible | heat sensitive phenotype of peb1

Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
NOT Enhancer of
Statement
Reference

tutl01085/tutl[+] is a non-enhancer of eye | heat sensitive phenotype of peb1

NOT Suppressor of
Statement
Reference

tutl01085/tutl[+] is a non-suppressor of eye | heat sensitive phenotype of peb1

Additional Comments
Genetic Interactions
Statement
Reference

The presence of ActβdsRNA.HL.Scer\UAS in tutl01085/+ mutant flies enhances the frequency of fusion between mutant R7 axonal terminals.

A tutl01085 heterozygous background enhances the frequency of tiling defects in Kap-α317-76 axonal somatic clones.

A Kap-α317-76 heterozygous background enhances the frequency of tiling defects in tutl01085 axonal somatic clones.

A tutlk14703 ; tutl01085 mutant background enhances the moderately rough eye phenotype observed upon expression of mblC.Scer\UAS under the control of Scer\GAL4hs.2sev.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Complements: slp105965. Complements: l(2)k05819k05819. Complements: Tps1k08903.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (15)