Allele Dmel\lwr⁰²⁸⁵⁸

General Information
Symbol	Dmel\lwr⁰²⁸⁵⁸	Species	D. melanogaster
Name		FlyBase ID	FBal0008009
Feature type	allele	Associated gene	Dmel\lwr
Also Known As	semi²⁸⁵⁸

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2L:541,010..541,010 2L:540,975..540,975 (Tanda, 1998.3.18, BDGP Project Members, 1994-1999, Epps and Tanda, 1998, Spradling et al., 1999, Apionishev et al., 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} is inserted 560 bp downstream of a putative polyadenylation signal for lwr. (Epps and Tanda, 1998)
Caused by insertion	P{PZ}lwr⁰²⁸⁵⁸ (Tanda, 1998.3.18, BDGP Project Members, 1994-1999, Epps and Tanda, 1998, Spradling et al., 1999, Apionishev et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| maternal effect \| recessive (Epps and Tanda, 1998) lethal \| recessive (Apionishev et al., 2001, Epps and Tanda, 1998, Spradling et al., 1999) lethal \| recessive \| rescuable maternal effect (Epps and Tanda, 1998)
Phenotype Manifest In
	embryonic abdominal segment (Epps and Tanda, 1998) embryonic epidermis (Epps and Tanda, 1998, Epps and Tanda, 1998) embryonic head (Epps and Tanda, 1998) embryonic thoracic segment (Epps and Tanda, 1998)
Detailed Description
	Statement Reference Lethality acts in the late embryonic to early larval stages. There is an apparent maternal requirement for lwr since embryos lacking only the zygotic gene product display phenotypes that resemble weak to strong alleles of hb, however when both the maternal and zygotic contributions of lwr are removed the embryos show a high penetrance of severe hunchback phenotypes. (Epps and Tanda, 1998) Lethality acts in the late embryonic or first larval instar stage. Cuticle phenotypes vary from minor, with only a part of T1 missing, to very severe, where embryos lack segments from T1 to A5, and parts of the head. The posterior part of the embryo is always normal. The variation in phenotype is due to variable maternal contribution of lwr function. Removal of maternal contribution increases the proportion of embryos with the severe defects to 60%, but not the severity of the defects. (Epps and Tanda, 1998) Embryos transheterozygous for lwr⁰⁵⁴⁸⁶ and lwr⁰²⁸⁵⁸ show cuticle patterns resembling those of lwr¹¹⁸ homozygotes and of lwr⁰⁵⁴⁸⁶/lwr¹¹⁸ transheterozygotes: a phenotype intermediate between the bcd and the hb mutant phenotypes. bcd protein is retained in the cytoplasm of lwr⁰⁵⁴⁸⁶/lwr⁰²⁸⁵⁸ transheterozygotes and lwr⁰⁵⁴⁸⁶ homozygotes. (Epps and Tanda, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference lwr⁰²⁸⁵⁸/smt3^k06307 is an enhancer of wing margin phenotype of ct⁶, mod(mdg4)^ul (Capelson and Corces, 2006)
NOT Enhancer of
Statement Reference lwr⁰²⁸⁵⁸ is a non-enhancer of wing vein \| ectopic phenotype of Cbl^Dv.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Robertson et al., 2000)
Suppressor of
Statement Reference lwr⁰²⁸⁵⁸/smt3^k06307 is a suppressor of adult cuticle phenotype of ct⁶, mod(mdg4)^ul (Capelson and Corces, 2006) lwr⁰²⁸⁵⁸/smt3^k06307 is a suppressor of adult cuticle phenotype of mod(mdg4)^ul, y² (Capelson and Corces, 2006) lwr⁰²⁸⁵⁸/smt3^k06307 is a suppressor of wing margin phenotype of ct⁶, mod(mdg4)^ul (Capelson and Corces, 2006)
NOT Suppressor of
Statement Reference lwr⁰²⁸⁵⁸ is a non-suppressor of wing vein \| ectopic phenotype of Cbl^Dv.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Robertson et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference The transheterozygote lwr⁰²⁸⁵⁸/smt3^k06307 suppresses the mod(mdg4)^ul suppression of the ct⁶ jagged wing phenotype, leading to a more jagged and discontinuous wing phenotype. The phenotype is also particularly pronounced in females, although it is detectable in both sexes. The transheterozygote lwr⁰²⁸⁵⁸/smt3^k06307 suppresses the mod(mdg4)^ul suppression of the y² cuticle phenotype, leading to a lighter coloration of the abdomen (due to a reduction in y expression). These phenotypic changes occur in approximately 30% of the mutant flies, with the remaining 70% displaying a continuum of less-pronounced effect. The phenotype is also particularly pronounced in females, although it is detectable in both sexes. (Capelson and Corces, 2006) A lwr[02858] mutant background does not affect the extent of ectopic wing veins or eye roughening seen in Scer\GAL4[GMR.PF] Cbl[Dv.Scer\UAS] mutants. (Robertson et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	lwr^4-3 (Apionishev et al., 2001) lwr⁵ (Apionishev et al., 2001) lwr^01519a (BDGP Project Members, 1994-1999) lwr⁰⁵⁴⁸⁶ (Apionishev et al., 2001, BDGP Project Members, 1994-1999)
Partially complements	lwr⁰⁵⁴⁸⁶ (Epps and Tanda, 1998, Epps and Tanda, 1998)
Rescued by	lwr⁰²⁸⁵⁸ is rescued by lwr^+LD12093 (Epps and Tanda, 1998)
Comments	Percent hatchability of embryos produced from a cross between lwr⁰⁵⁴⁸⁶ and lwr⁰²⁸⁵⁸ is 75%, whereas hatchability from a cross between ush² and lwr⁰²⁸⁵⁸ is 90%. (Epps and Tanda, 1998)
Stocks ( 0 )

Notes on Origin
Discoverer	A. Spradling.

Comments
	Precise excision of the P{PZ} element reverts lethal mutant phenotype to viability. (Epps and Tanda, 1998) Likely to be a regulatory mutant that may specifically affect transcription during early embryogenesis. Complements ush². Two excision alleles derived from lwr⁰²⁸⁵⁸ show greater degree of lethality when transheterozygous with lwr⁰⁵⁴⁸⁶ than did lwr⁰²⁸⁵⁸. (Epps and Tanda, 1998) Complements: ex⁰¹²⁷⁰. Complements: ex⁰²²⁷⁸. Complements: ds⁰⁵¹⁴². Complements: Gsc⁰⁵³⁴¹. Complements: α-Adaptin⁰⁶⁶⁹⁴. Complements: S^k06101. Complements: l(2)k08915^k08915. Complements: l(2)k09610^k09610. Complements: Nle^k13714. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	l(2)02858 (Spradling et al., 1999) l(2)02858⁰²⁸⁵⁸ lwr⁰²⁸⁵⁸ lwr²⁸⁵⁸ (Capelson and Corces, 2006) lwr^semi2858 (Apionishev et al., 2001) semi²⁸⁵⁸ (Epps and Tanda, 1998, Epps and Tanda, 1998) semi⁰²⁸⁵⁸ (Robertson et al., 2000)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Capelson and Corces, 2006, EMBO J. 25(9): 1906--1914 SUMO conjugation attenuates the activity of the gypsy chromatin insulator. [FBrf0193462] Apionishev et al., 2001, Genes Cells 6(3): 215--224 The Drosophila UBC9 homologue lesswright mediates the disjunction of homologues in meiosis I. [FBrf0134637] Robertson et al., 2000, Oncogene 19(29): 3299--3308 A Drosophila analogue of v-Cbl is a dominant-negative oncoprotein in vivo. [FBrf0130045] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Epps and Tanda, 1998, Curr. Biol. 8(23): 1277--1280 The Drosophila semushi mutation blocks nuclear import of bicoid during embryogenesis. [FBrf0105226]
Supplementary material	Epps and Tanda, 1998, Curr. Biol. 8(23): The Drosophila semushi mutation blocks nuclear import of bicoid during embryogenesis. [FBrf0107698]
Personal communication to FlyBase	Tanda, 1998.3.18, ADRC: 254B. ADRC: 254B. [FBrf0102086] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Epps and Tanda, 1998, A. Dros. Res. Conf. 39: 255C hunchback-like (hbl) encodes a ubiquitin conjugating enzyme and potentially functions as a Gap gene. [FBrf0101292]

Allele Dmel\lwr02858

Allele Dmel\lwr⁰²⁸⁵⁸