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General Information
Symbol
Dmel\bs03267
Species
D. melanogaster
Name
FlyBase ID
FBal0008021
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
bsP1292, pruned1, bs-lacZ, P{PZ}bs03267
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} is inserted in the first exon of the bs gene, 350bp upstream of the assigned translation start site.

Insertion components
P{PZ}bs03267
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

heterozygous bs03267 flies have small patches of ectopic vein in the vicininty of L2 and L5.

Heterozygotes have a blistered wing and ectopic vein phenotype.

Trachea within bs03267 somatic clones are unable to produce terminal branches in a cell autonomous manner, however, bs+ tracheal cells in neighbouring segments grow into the detracheated region sprouting about 40% more terminal branches than normal. bs+ tracheal cells not bordering the cone are unaffected.

Some escapers reach the adult stage. Pharate adult homozygotes show the whole wing blade transformed into corrugated vein tissue whereas the hinge region is almost intact. Wing margin sensory organs and the pattern of campaniformia sensillae along the normal L3 are in the normal positions. Some ectopic bristles and sensillae occasionally appear in internal regions of the wing. Legs often show shortening of the tarsal segments and bent femur. Clones of homozygous mutant tissue induced at 48-72 hours AEL are of normal size and are concentrated in the proximity of vein positions. Mutant cells autonomously differentiate vein tissue. Clones have smooth borders, in contract to control clones with indented borders. Clones induced in a M(2)58Funspecified background can entirely surround wild type tissue which differentiates as intervein. Heterozygous adults show small patches of ectopic veins in the L2 and L5 proximity. bs03267/bs2 is a viable combination that displays almost entirely wing vein tissue in the wing.

Major tracheal branches and connections between branches are normal, but terminal branches were truncated or absent. In larvae, the parental branches end abruptly without ramification. Surviving larvae can move about but are sluggish. Many contain dark tissue masses typically associated with the fat body, possibly due to necrosis of the fat body caused by inadequate oxygen supply. The terminal cells of mutant embryos do not have long projections extending to their targets. No muscle defects can be detected in mutant embryos.

Behaves as a genetic null for tracheal function. Clonal analysis in the wing shows that bs function is necessary for intervein formation. The earlier the clones are induced, the proportionally greater contribution of vein tissue to the remaining wing, as 'intervein' cells acquire vein cell morphology. This phenotype is cell autonomous. Large mutant clones produce blisters that are generally limited to the mutant tissue. Blisters form even when the opposite wing surface is differentiated as intervein.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

bs03267/bs[+] is an enhancer | cold sensitive of visible phenotype of br1

bs03267/bs[+] is an enhancer of visible | heat sensitive phenotype of HC2.hs

bs03267/bs[+] is an enhancer of visible phenotype of Mer3

Suppressor of
Statement
Reference

bs03267/bs[+] is a suppressor of visible phenotype of Mer3

bs03267 is a suppressor of visible phenotype of cswlf

bs03267/bs2 is a suppressor of visible phenotype of rho9/rhove-1, vnM2/vn1

NOT Suppressor of
Statement
Reference

bs03267 is a non-suppressor of cell migration defective phenotype of ptcD130

Other
Phenotype Manifest In
Suppressed by
Statement
Reference

bs03267 has wing vein | ectopic phenotype, suppressible | partially by brm2

Enhancer of
Statement
Reference

bs03267/bs[+] is an enhancer of submarginal cell phenotype of ash2S112411

bs03267/bs[+] is an enhancer of discal cell phenotype of ash2S112411

bs03267/bs[+] is an enhancer of wing vein | ectopic phenotype of ash2S112411

bs03267/bs[+] is an enhancer of crossvein | ectopic phenotype of ash2S112411

bs03267/bs[+] is an enhancer of wing vein L2 phenotype of ash2S112411

bs03267/bs[+] is an enhancer of wing vein | ectopic phenotype of Snr1E1

bs03267/bs[+] is an enhancer | cold sensitive of leg phenotype of br1

bs03267/bs[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of HC2.hs

bs03267/bs[+] is an enhancer of wing | heat sensitive phenotype of HC2.hs

bs03267/bs[+] is an enhancer of wing vein L1 phenotype of Adv1

bs03267/bs[+] is an enhancer of wing vein L2 phenotype of Adv1

bs03267/bs[+] is an enhancer of wing vein | ectopic phenotype of Adv1

bs03267 is an enhancer of phenotype of Nnd-3

bs03267 is an enhancer of phenotype of rhohs.PSt

bs03267 is an enhancer of phenotype of DlM1

bs03267 is an enhancer of phenotype of emc1

bs03267 is an enhancer of phenotype of emcP5c

bs03267 is an enhancer of phenotype of px72

bs03267 is an enhancer of phenotype of rlSem

Suppressor of
Statement
Reference

bs03267/bs[+] is a suppressor | partially of wing phenotype of Mer3

bs03267 is a suppressor of ommatidium phenotype of cswlf

bs03267 is a suppressor of wing vein phenotype of cswlf

bs03267 is a suppressor of phenotype of vnddd-3

bs03267 is a suppressor of phenotype of Egfrf37

bs03267 is a suppressor of phenotype of vn1

bs03267 is a suppressor of phenotype of rl1

bs03267/bs2 is a suppressor of wing vein phenotype of rho9/rhove-1, vnM2/vn1

bs03267 is a suppressor of phenotype of Egfrt1

bs03267 is a suppressor of phenotype of vnM2

bs03267 is a suppressor of phenotype of rl10

bs03267 is a suppressor of phenotype of NAx-16

NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

ptcD130 mutant ganglionic branch cells are still unable to migrate towards the ventral nerve cord, in a bs03267 mutant background.

Heterozygous bs03267 flies have small patches of ectopic vein in the vicininty of L2 and L5. If these flies are also homozygous for ash2S112411 their wings are small, blistered and exhibit localised reduction and loss of intervein betwee L2 and L3 and between L4 and L5 and ectopic crossveins. Fusions occur between L2 and L3 and between L4 and L5, particularly proximally.

Dominantly enhances the frequency of br1 mutant animals with malformed legs. This phenotype is cold-sensitive; at 18oC, 73% of br1/Y ; bs03267/+ animals have malformed legs, at 21oC, the frequency of malformed legs is 15%.

The increase in the size of the wing blade seen in Mer3/Y flies is partially suppressed by bs03267/+.

bs03267/+ ; emc1/emc11 flies show an extra wing vein phenotype.

Transheterozygotes of px72 with bs03267 show ectopic venation.

Hemizygosity for bs totally suppresses the lack of vein L4 phenotype of loss of function Egfr, rl, vn and rho mutants and greatly enhances the amount of ectopic vein in rlSem and rhohs.PSt, even in the absence of heat shock. Double mutant wing clones with Egfrf37 occupy vein territories like those for bs03267 but with a frequency and size similar to Egfrf37 controls. Double mutant cells differentiate autonomously into pigmented, corrugated and compacted cells characteristic of the vein histotype. bs03267/bs2; rho9, vnM2/rhove-1, vn1 triple mutants show an almost wild type wing vein pattern.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Excision of the P{PZ} can be accompanied by reversion of the mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (25)