FlyBase Allele Report: Dmel\bs[03267]

General Information

Symbol

Dmel\bs⁰³²⁶⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0008021

Feature type

allele

Associated gene

Dmel\bs

Associated Insertion(s)

P{PZ}bs⁰³²⁶⁷

Carried in Construct

Also Known As

bs^P1292, pruned¹, bs-lacZ, P{PZ}bs⁰³²⁶⁷

Genomic Maps

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Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Jarecki et al., 1999)

Mutagen

P-element activity

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable element insertion site

2R:24,343,737..24,343,744 [+]

(Miao and Hayashi, 2016, Ghabrial et al., 2011, Marenda et al., 2006, Nussbaumer et al., 2000, Wilk et al., 2000, Baonza and Garcia-Bellido, 1999, Spradling et al., 1999, Roch et al., 1998, Guillemin et al., 1996, Samakovlis et al., 1996, BDGP Project Members, 1994-1999)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

P{PZ} is inserted in the first exon of the bs gene, 350bp upstream of the assigned translation start site.

(Guillemin et al., 1996)

Insertion components

P{PZ}bs⁰³²⁶⁷

Product class / Tool use(s)

Encoded product / tool

Tagged with

nuclear protein localization tag

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal | recessive

(Firth et al., 2000, Spradling et al., 1999, Roch et al., 1998, Guillemin et al., 1996)

viable (with bs²)

(Roch et al., 1998)

visible (with bs²)

(Roch et al., 1998)

visible | dominant

(Firth et al., 2000, Roch et al., 1998)

visible | recessive

(Matakatsu et al., 1999, Roch et al., 1998)

Phenotype Manifest In

embryonic/larval tracheal system

(Jarecki et al., 1999)

embryonic/larval tracheal system | somatic clone

(Jarecki et al., 1999)

femur

(Roch et al., 1998)

macrochaeta | ectopic

(Roch et al., 1998)

sensillum campaniformium | ectopic

(Roch et al., 1998)

tarsal segment

(Roch et al., 1998)

trachea

(Guillemin et al., 1996)

tracheal metamere 3

(Jarecki et al., 1999)

tracheal metamere 3 | somatic clone

(Jarecki et al., 1999)

wing

(Firth et al., 2000, Roch et al., 1998, Montagne et al., 1996)

wing (with bs²)

(Roch et al., 1998)

wing & macrochaeta

(Roch et al., 1998)

wing cell

(Montagne et al., 1996)

wing sensillum | ectopic

(Roch et al., 1998)

wing vein | ectopic

(Angulo et al., 2004, Marenda et al., 2004, Firth et al., 2000, Roch et al., 1998)

wing vein | ectopic (with bs²)

(Roch et al., 1998)

wing vein L3 & sensillum campaniformium

(Roch et al., 1998)

Detailed Description

Statement

Reference

heterozygous bs⁰³²⁶⁷ flies have small patches of ectopic vein in the vicininty of L2 and L5.

(Angulo et al., 2004)

Heterozygotes have a blistered wing and ectopic vein phenotype.

(Firth et al., 2000)

Trachea within bs⁰³²⁶⁷ somatic clones are unable to produce terminal branches in a cell autonomous manner, however, bs⁺ tracheal cells in neighbouring segments grow into the detracheated region sprouting about 40% more terminal branches than normal. bs⁺ tracheal cells not bordering the cone are unaffected.

(Jarecki et al., 1999)

Some escapers reach the adult stage. Pharate adult homozygotes show the whole wing blade transformed into corrugated vein tissue whereas the hinge region is almost intact. Wing margin sensory organs and the pattern of campaniformia sensillae along the normal L3 are in the normal positions. Some ectopic bristles and sensillae occasionally appear in internal regions of the wing. Legs often show shortening of the tarsal segments and bent femur. Clones of homozygous mutant tissue induced at 48-72 hours AEL are of normal size and are concentrated in the proximity of vein positions. Mutant cells autonomously differentiate vein tissue. Clones have smooth borders, in contract to control clones with indented borders. Clones induced in a M(2)58F^unspecified background can entirely surround wild type tissue which differentiates as intervein. Heterozygous adults show small patches of ectopic veins in the L2 and L5 proximity. bs⁰³²⁶⁷/bs² is a viable combination that displays almost entirely wing vein tissue in the wing.

(Roch et al., 1998)

Major tracheal branches and connections between branches are normal, but terminal branches were truncated or absent. In larvae, the parental branches end abruptly without ramification. Surviving larvae can move about but are sluggish. Many contain dark tissue masses typically associated with the fat body, possibly due to necrosis of the fat body caused by inadequate oxygen supply. The terminal cells of mutant embryos do not have long projections extending to their targets. No muscle defects can be detected in mutant embryos.

(Guillemin et al., 1996)

Behaves as a genetic null for tracheal function. Clonal analysis in the wing shows that bs function is necessary for intervein formation. The earlier the clones are induced, the proportionally greater contribution of vein tissue to the remaining wing, as 'intervein' cells acquire vein cell morphology. This phenotype is cell autonomous. Large mutant clones produce blisters that are generally limited to the mutant tissue. Blisters form even when the opposite wing surface is differentiated as intervein.

(Montagne et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

bs⁰³²⁶⁷/bs[+] is an enhancer | cold sensitive of visible phenotype of br¹

(Ward et al., 2003)

bs⁰³²⁶⁷/bs[+] is an enhancer of visible | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

bs⁰³²⁶⁷/bs[+] is an enhancer of visible phenotype of Mer³

(LaJeunesse et al., 2001)

Suppressor of

Statement

Reference

bs⁰³²⁶⁷/bs[+] is a suppressor of visible phenotype of Mer³

(LaJeunesse et al., 2001)

bs⁰³²⁶⁷ is a suppressor of visible phenotype of csw^lf

(Firth et al., 2000)

bs⁰³²⁶⁷/bs² is a suppressor of visible phenotype of rho⁹/rho^ve-1, vn^M2/vn¹

(Roch et al., 1998)

NOT Suppressor of

Statement

Reference

bs⁰³²⁶⁷ is a non-suppressor of cell migration defective phenotype of ptc^D130

(Butí et al., 2014)

Other

Statement

Reference

bs⁰³²⁶⁷, emc¹/emc¹¹ has visible | dominant phenotype

(Baonza and Garcia-Bellido, 1999)

bs⁰³²⁶⁷, emc¹/emc¹¹ has visible phenotype

(Baonza and Garcia-Bellido, 1999)

bs⁰³²⁶⁷, px⁷² has visible phenotype

(Matakatsu et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

bs⁰³²⁶⁷ has wing vein | ectopic phenotype, suppressible | partially by brm²

(Marenda et al., 2004)

Enhancer of

Statement

Reference

bs⁰³²⁶⁷/bs[+] is an enhancer of submarginal cell phenotype of ash2^S112411

(Angulo et al., 2004)

bs⁰³²⁶⁷/bs[+] is an enhancer of discal cell phenotype of ash2^S112411

(Angulo et al., 2004)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing vein | ectopic phenotype of ash2^S112411

(Angulo et al., 2004)

bs⁰³²⁶⁷/bs[+] is an enhancer of crossvein | ectopic phenotype of ash2^S112411

(Angulo et al., 2004)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing vein L2 phenotype of ash2^S112411

(Angulo et al., 2004)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

bs⁰³²⁶⁷/bs[+] is an enhancer | cold sensitive of leg phenotype of br¹

(Ward et al., 2003)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing vein L1 phenotype of Adv¹

(Srivastava et al., 2002)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing vein L2 phenotype of Adv¹

(Srivastava et al., 2002)

bs⁰³²⁶⁷/bs[+] is an enhancer of wing vein | ectopic phenotype of Adv¹

(Srivastava et al., 2002)

bs⁰³²⁶⁷ is an enhancer of phenotype of N^nd-3

(Roch et al., 1998)

bs⁰³²⁶⁷ is an enhancer of phenotype of rho^hs.PSt

(Roch et al., 1998)

bs⁰³²⁶⁷ is an enhancer of phenotype of Dl^M1

(Roch et al., 1998)

bs⁰³²⁶⁷ is an enhancer of phenotype of emc¹

(Roch et al., 1998)

bs⁰³²⁶⁷ is an enhancer of phenotype of emc^P5c

(Roch et al., 1998)

bs⁰³²⁶⁷ is an enhancer of phenotype of px⁷²

(Roch et al., 1998)

bs⁰³²⁶⁷ is an enhancer of phenotype of rl^Sem

(Roch et al., 1998)

Suppressor of

Statement

Reference

bs⁰³²⁶⁷/bs[+] is a suppressor | partially of wing phenotype of Mer³

(LaJeunesse et al., 2001)

bs⁰³²⁶⁷ is a suppressor of ommatidium phenotype of csw^lf

(Firth et al., 2000)

bs⁰³²⁶⁷ is a suppressor of wing vein phenotype of csw^lf

(Firth et al., 2000)

bs⁰³²⁶⁷ is a suppressor of phenotype of vn^ddd-3

(Roch et al., 1998)

bs⁰³²⁶⁷ is a suppressor of phenotype of Egfr^f37

(Roch et al., 1998)

bs⁰³²⁶⁷ is a suppressor of phenotype of vn¹

(Roch et al., 1998)

bs⁰³²⁶⁷ is a suppressor of phenotype of rl¹

(Roch et al., 1998)

bs⁰³²⁶⁷/bs² is a suppressor of wing vein phenotype of rho⁹/rho^ve-1, vn^M2/vn¹

(Roch et al., 1998)

bs⁰³²⁶⁷ is a suppressor of phenotype of Egfr^t1

(Roch et al., 1998)

bs⁰³²⁶⁷ is a suppressor of phenotype of vn^M2

(Roch et al., 1998)

bs⁰³²⁶⁷ is a suppressor of phenotype of rl¹⁰

(Roch et al., 1998)

bs⁰³²⁶⁷ is a suppressor of phenotype of N^Ax-16

(Roch et al., 1998)

NOT Suppressor of

Statement

Reference

bs⁰³²⁶⁷ is a non-suppressor of embryonic/larval tracheal system phenotype of ptc^D130

(Butí et al., 2014)

bs⁰³²⁶⁷ is a non-suppressor of embryonic/larval ganglionic branch phenotype of ptc^D130

(Butí et al., 2014)

Other

Statement

Reference

bs⁰³²⁶⁷, emc¹/emc¹¹ has wing vein | ectopic phenotype

(Baonza and Garcia-Bellido, 1999)

bs⁰³²⁶⁷, px⁷² has wing phenotype

(Matakatsu et al., 1999)

bs⁰³²⁶⁷, px⁷² has wing vein | ectopic phenotype

(Matakatsu et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

ptc^D130 mutant ganglionic branch cells are still unable to migrate towards the ventral nerve cord, in a bs⁰³²⁶⁷ mutant background.

(Butí et al., 2014)

Heterozygous bs⁰³²⁶⁷ flies have small patches of ectopic vein in the vicininty of L2 and L5. If these flies are also homozygous for ash2^S112411 their wings are small, blistered and exhibit localised reduction and loss of intervein betwee L2 and L3 and between L4 and L5 and ectopic crossveins. Fusions occur between L2 and L3 and between L4 and L5, particularly proximally.

(Angulo et al., 2004)

Dominantly enhances the frequency of br¹ mutant animals with malformed legs. This phenotype is cold-sensitive; at 18^oC, 73% of br¹/Y ; bs⁰³²⁶⁷/+ animals have malformed legs, at 21^oC, the frequency of malformed legs is 15%.

(Ward et al., 2003)

The increase in the size of the wing blade seen in Mer³/Y flies is partially suppressed by bs⁰³²⁶⁷/+.

(LaJeunesse et al., 2001)

bs⁰³²⁶⁷/+ ; emc¹/emc¹¹ flies show an extra wing vein phenotype.

(Baonza and Garcia-Bellido, 1999)

Transheterozygotes of px⁷² with bs⁰³²⁶⁷ show ectopic venation.

(Matakatsu et al., 1999)

Hemizygosity for bs totally suppresses the lack of vein L4 phenotype of loss of function Egfr, rl, vn and rho mutants and greatly enhances the amount of ectopic vein in rl^Sem and rho^hs.PSt, even in the absence of heat shock. Double mutant wing clones with Egfr^f37 occupy vein territories like those for bs⁰³²⁶⁷ but with a frequency and size similar to Egfr^f37 controls. Double mutant cells differentiate autonomously into pigmented, corrugated and compacted cells characteristic of the vein histotype. bs⁰³²⁶⁷/bs²; rho⁹, vn^M2/rho^ve-1, vn¹ triple mutants show an almost wild type wing vein pattern.

(Roch et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

bs^Ebr292

(Ward et al., 2003)

bs^k07909

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

83157

cn^* P{PZ}bs⁰³²⁶⁷/CyO

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(2)04012a^04012a. Complements: Nop60B^k05318. Complements: Mov34^k08003.

(BDGP Project Members, 1994-1999)

Excision of the P{PZ} can be accompanied by reversion of the mutant phenotype.

(Guillemin et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

blistered^pruned1

(Jarecki et al., 1999)

bs⁰³²⁶⁷

(Angulo et al., 2004)

bs^P1292

(Marenda et al., 2004, Johannes and Preiss, 2002, Brentrup et al., 2000, Baonza and Garcia-Bellido, 1999, Roch et al., 1998)

bs^PZ

(Miao and Hayashi, 2016)

bs^k03267

(Ward et al., 2003)

bs^l(2)03267

(Matakatsu et al., 1999, Beaton, 1997.12.27)

l(2)03267

(Spradling et al., 1999)

pruned¹

(Guillemin et al., 1996, Montagne et al., 1996)

Name Synonyms

pruned1

(Butí et al., 2014)

Secondary FlyBase IDs

References (26)

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