FB2021_06, released December 14, 2021

Allele: Dmel\bs03267

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General Information
Symbol
Dmel\bs03267
Species
D. melanogaster
Name
FlyBase ID
FBal0008021
Feature type
allele
Associated gene
Dmel\bs
Associated Insertion(s)
P{PZ}bs03267
Carried in Construct
Also Known As
bsP1292, pruned1, bs-lacZ, P{PZ}bs03267
Key Links
Genomic Maps
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Jarecki et al., 1999)
Mutagen
P-element activity
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
transposable_element_insertion_site
2R:24,343,737..24,343,744 [+]
(Ricolo and Araujo, 2020, Miao and Hayashi, 2016, Ghabrial et al., 2011, Marenda et al., 2006, Nussbaumer et al., 2000, Wilk et al., 2000, Baonza and Garcia-Bellido, 1999, Spradling et al., 1999, Roch et al., 1998, Guillemin et al., 1996, Samakovlis et al., 1996, BDGP Project Members, 1994-1999)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      P{PZ} is inserted in the first exon of the bs gene, 350bp upstream of the assigned translation start site.

      (Guillemin et al., 1996)
      Insertion components
      P{PZ}bs03267
      Product class / Tool use(s)
      Encoded product / tool
      lacZ
      Tagged with
      Tag:NLS(P)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      heterozygous bs03267 flies have small patches of ectopic vein in the vicininty of L2 and L5.

      (Angulo et al., 2004)

      Heterozygotes have a blistered wing and ectopic vein phenotype.

      (Firth et al., 2000)

      Trachea within bs03267 somatic clones are unable to produce terminal branches in a cell autonomous manner, however, bs+ tracheal cells in neighbouring segments grow into the detracheated region sprouting about 40% more terminal branches than normal. bs+ tracheal cells not bordering the cone are unaffected.

      (Jarecki et al., 1999)

      Some escapers reach the adult stage. Pharate adult homozygotes show the whole wing blade transformed into corrugated vein tissue whereas the hinge region is almost intact. Wing margin sensory organs and the pattern of campaniformia sensillae along the normal L3 are in the normal positions. Some ectopic bristles and sensillae occasionally appear in internal regions of the wing. Legs often show shortening of the tarsal segments and bent femur. Clones of homozygous mutant tissue induced at 48-72 hours AEL are of normal size and are concentrated in the proximity of vein positions. Mutant cells autonomously differentiate vein tissue. Clones have smooth borders, in contract to control clones with indented borders. Clones induced in a M(2)58Funspecified background can entirely surround wild type tissue which differentiates as intervein. Heterozygous adults show small patches of ectopic veins in the L2 and L5 proximity. bs03267/bs2 is a viable combination that displays almost entirely wing vein tissue in the wing.

      (Roch et al., 1998)

      Major tracheal branches and connections between branches are normal, but terminal branches were truncated or absent. In larvae, the parental branches end abruptly without ramification. Surviving larvae can move about but are sluggish. Many contain dark tissue masses typically associated with the fat body, possibly due to necrosis of the fat body caused by inadequate oxygen supply. The terminal cells of mutant embryos do not have long projections extending to their targets. No muscle defects can be detected in mutant embryos.

      (Guillemin et al., 1996)

      Behaves as a genetic null for tracheal function. Clonal analysis in the wing shows that bs function is necessary for intervein formation. The earlier the clones are induced, the proportionally greater contribution of vein tissue to the remaining wing, as 'intervein' cells acquire vein cell morphology. This phenotype is cell autonomous. Large mutant clones produce blisters that are generally limited to the mutant tissue. Blisters form even when the opposite wing surface is differentiated as intervein.

      (Montagne et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      bs03267/bs[+] is an enhancer | cold sensitive of visible phenotype of br1

      (Ward et al., 2003)

      bs03267/bs[+] is an enhancer of visible | heat sensitive phenotype of HC2.hs

      (Johannes and Preiss, 2002)

      bs03267/bs[+] is an enhancer of visible phenotype of Mer3

      (LaJeunesse et al., 2001)
      Suppressor of
      Statement
      Reference

      bs03267/bs[+] is a suppressor of visible phenotype of Mer3

      (LaJeunesse et al., 2001)

      bs03267 is a suppressor of visible phenotype of cswlf

      (Firth et al., 2000)

      bs03267/bs2 is a suppressor of visible phenotype of rho9/rhove-1, vnM2/vn1

      (Roch et al., 1998)
      NOT Suppressor of
      Statement
      Reference

      bs03267 is a non-suppressor of abnormal cell migration phenotype of ptcD130

      (Butí et al., 2014)
      Other
      Phenotype Manifest In
      Suppressed by
      Enhancer of
      Statement
      Reference

      bs03267/bs[+] is an enhancer of submarginal cell phenotype of ash2S112411

      (Angulo et al., 2004)

      bs03267/bs[+] is an enhancer of discal cell phenotype of ash2S112411

      (Angulo et al., 2004)

      bs03267/bs[+] is an enhancer of wing vein | ectopic phenotype of ash2S112411

      (Angulo et al., 2004)

      bs03267/bs[+] is an enhancer of crossvein | ectopic phenotype of ash2S112411

      (Angulo et al., 2004)

      bs03267/bs[+] is an enhancer of wing vein L2 phenotype of ash2S112411

      (Angulo et al., 2004)

      bs03267/bs[+] is an enhancer of wing vein | ectopic phenotype of Snr1E1

      (Marenda et al., 2004)

      bs03267/bs[+] is an enhancer | cold sensitive of leg phenotype of br1

      (Ward et al., 2003)

      bs03267/bs[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of HC2.hs

      (Johannes and Preiss, 2002)

      bs03267/bs[+] is an enhancer of wing | heat sensitive phenotype of HC2.hs

      (Johannes and Preiss, 2002)

      bs03267/bs[+] is an enhancer of wing vein L1 phenotype of Adv1

      (Srivastava et al., 2002)

      bs03267/bs[+] is an enhancer of wing vein L2 phenotype of Adv1

      (Srivastava et al., 2002)

      bs03267/bs[+] is an enhancer of wing vein | ectopic phenotype of Adv1

      (Srivastava et al., 2002)

      bs03267 is an enhancer of phenotype of Nnd-3

      (Roch et al., 1998)

      bs03267 is an enhancer of phenotype of rhohs.PSt

      (Roch et al., 1998)

      bs03267 is an enhancer of phenotype of DlM1

      (Roch et al., 1998)

      bs03267 is an enhancer of phenotype of emc1

      (Roch et al., 1998)

      bs03267 is an enhancer of phenotype of emcP5c

      (Roch et al., 1998)

      bs03267 is an enhancer of phenotype of px72

      (Roch et al., 1998)

      bs03267 is an enhancer of phenotype of rlSem

      (Roch et al., 1998)
      Suppressor of
      Statement
      Reference

      bs03267/bs[+] is a suppressor | partially of wing phenotype of Mer3

      (LaJeunesse et al., 2001)

      bs03267 is a suppressor of ommatidium phenotype of cswlf

      (Firth et al., 2000)

      bs03267 is a suppressor of wing vein phenotype of cswlf

      (Firth et al., 2000)

      bs03267 is a suppressor of phenotype of vnddd-3

      (Roch et al., 1998)

      bs03267 is a suppressor of phenotype of Egfrf37

      (Roch et al., 1998)

      bs03267 is a suppressor of phenotype of vn1

      (Roch et al., 1998)

      bs03267 is a suppressor of phenotype of rl1

      (Roch et al., 1998)

      bs03267/bs2 is a suppressor of wing vein phenotype of rho9/rhove-1, vnM2/vn1

      (Roch et al., 1998)

      bs03267 is a suppressor of phenotype of Egfrt1

      (Roch et al., 1998)

      bs03267 is a suppressor of phenotype of vnM2

      (Roch et al., 1998)

      bs03267 is a suppressor of phenotype of rl10

      (Roch et al., 1998)

      bs03267 is a suppressor of phenotype of NAx-16

      (Roch et al., 1998)
      NOT Suppressor of
      Statement
      Reference

      bs03267 is a non-suppressor of embryonic/larval tracheal system phenotype of ptcD130

      (Butí et al., 2014)

      bs03267 is a non-suppressor of embryonic/larval ganglionic branch phenotype of ptcD130

      (Butí et al., 2014)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      ptcD130 mutant ganglionic branch cells are still unable to migrate towards the ventral nerve cord, in a bs03267 mutant background.

      (Butí et al., 2014)

      Heterozygous bs03267 flies have small patches of ectopic vein in the vicininty of L2 and L5. If these flies are also homozygous for ash2S112411 their wings are small, blistered and exhibit localised reduction and loss of intervein betwee L2 and L3 and between L4 and L5 and ectopic crossveins. Fusions occur between L2 and L3 and between L4 and L5, particularly proximally.

      (Angulo et al., 2004)

      Dominantly enhances the frequency of br1 mutant animals with malformed legs. This phenotype is cold-sensitive; at 18oC, 73% of br1/Y ; bs03267/+ animals have malformed legs, at 21oC, the frequency of malformed legs is 15%.

      (Ward et al., 2003)

      The increase in the size of the wing blade seen in Mer3/Y flies is partially suppressed by bs03267/+.

      (LaJeunesse et al., 2001)

      bs03267/+ ; emc1/emc11 flies show an extra wing vein phenotype.

      (Baonza and Garcia-Bellido, 1999)

      Transheterozygotes of px72 with bs03267 show ectopic venation.

      (Matakatsu et al., 1999)

      Hemizygosity for bs totally suppresses the lack of vein L4 phenotype of loss of function Egfr, rl, vn and rho mutants and greatly enhances the amount of ectopic vein in rlSem and rhohs.PSt, even in the absence of heat shock. Double mutant wing clones with Egfrf37 occupy vein territories like those for bs03267 but with a frequency and size similar to Egfrf37 controls. Double mutant cells differentiate autonomously into pigmented, corrugated and compacted cells characteristic of the vein histotype. bs03267/bs2; rho9, vnM2/rhove-1, vn1 triple mutants show an almost wild type wing vein pattern.

      (Roch et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      A. Spradling.

      Comments
      Comments

      Complements: l(2)04012a04012a. Complements: Nop60Bk05318. Complements: Mov34k08003.

      (BDGP Project Members, 1994-1999)

      Excision of the P{PZ} can be accompanied by reversion of the mutant phenotype.

      (Guillemin et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      References (27)