wing & macrochaeta
wing vein L3 & sensillum campaniformium
heterozygous bs03267 flies have small patches of ectopic vein in the vicininty of L2 and L5.
Heterozygotes have a blistered wing and ectopic vein phenotype.
Trachea within bs03267 somatic clones are unable to produce terminal branches in a cell autonomous manner, however, bs+ tracheal cells in neighbouring segments grow into the detracheated region sprouting about 40% more terminal branches than normal. bs+ tracheal cells not bordering the cone are unaffected.
Some escapers reach the adult stage. Pharate adult homozygotes show the whole wing blade transformed into corrugated vein tissue whereas the hinge region is almost intact. Wing margin sensory organs and the pattern of campaniformia sensillae along the normal L3 are in the normal positions. Some ectopic bristles and sensillae occasionally appear in internal regions of the wing. Legs often show shortening of the tarsal segments and bent femur. Clones of homozygous mutant tissue induced at 48-72 hours AEL are of normal size and are concentrated in the proximity of vein positions. Mutant cells autonomously differentiate vein tissue. Clones have smooth borders, in contract to control clones with indented borders. Clones induced in a M(2)58Funspecified background can entirely surround wild type tissue which differentiates as intervein. Heterozygous adults show small patches of ectopic veins in the L2 and L5 proximity. bs03267/bs2 is a viable combination that displays almost entirely wing vein tissue in the wing.
Major tracheal branches and connections between branches are normal, but terminal branches were truncated or absent. In larvae, the parental branches end abruptly without ramification. Surviving larvae can move about but are sluggish. Many contain dark tissue masses typically associated with the fat body, possibly due to necrosis of the fat body caused by inadequate oxygen supply. The terminal cells of mutant embryos do not have long projections extending to their targets. No muscle defects can be detected in mutant embryos.
Behaves as a genetic null for tracheal function. Clonal analysis in the wing shows that bs function is necessary for intervein formation. The earlier the clones are induced, the proportionally greater contribution of vein tissue to the remaining wing, as 'intervein' cells acquire vein cell morphology. This phenotype is cell autonomous. Large mutant clones produce blisters that are generally limited to the mutant tissue. Blisters form even when the opposite wing surface is differentiated as intervein.
bs03267 is a non-suppressor of cell migration defective phenotype of ptcD130
bs03267/bs[+] is an enhancer of submarginal cell phenotype of ash2S112411
bs03267/bs[+] is an enhancer of discal cell phenotype of ash2S112411
bs03267/bs[+] is an enhancer of wing vein | ectopic phenotype of ash2S112411
bs03267/bs[+] is an enhancer of crossvein | ectopic phenotype of ash2S112411
bs03267/bs[+] is an enhancer of wing vein L2 phenotype of ash2S112411
bs03267/bs[+] is an enhancer | cold sensitive of leg phenotype of br1
bs03267/bs[+] is an enhancer of wing vein L1 phenotype of Adv1
bs03267/bs[+] is an enhancer of wing vein L2 phenotype of Adv1
bs03267 is a non-suppressor of embryonic/larval tracheal system phenotype of ptcD130
bs03267 is a non-suppressor of embryonic/larval ganglionic branch phenotype of ptcD130
Heterozygous bs03267 flies have small patches of ectopic vein in the vicininty of L2 and L5. If these flies are also homozygous for ash2S112411 their wings are small, blistered and exhibit localised reduction and loss of intervein betwee L2 and L3 and between L4 and L5 and ectopic crossveins. Fusions occur between L2 and L3 and between L4 and L5, particularly proximally.
Hemizygosity for bs totally suppresses the lack of vein L4 phenotype of loss of function Egfr, rl, vn and rho mutants and greatly enhances the amount of ectopic vein in rlSem and rhohs.PSt, even in the absence of heat shock. Double mutant wing clones with Egfrf37 occupy vein territories like those for bs03267 but with a frequency and size similar to Egfrf37 controls. Double mutant cells differentiate autonomously into pigmented, corrugated and compacted cells characteristic of the vein histotype. bs03267/bs2; rho9, vnM2/rhove-1, vn1 triple mutants show an almost wild type wing vein pattern.
A. Spradling.
Complements: l(2)04012a04012a. Complements: Nop60Bk05318. Complements: Mov34k08003.
Excision of the P{PZ} can be accompanied by reversion of the mutant phenotype.