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General Information
Symbol
Dmel\vari03953b
Species
D. melanogaster
Name
FlyBase ID
FBal0008046
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
vari3953b
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

17bp deletion in the third common intron; flanking sequences are CTTGC_AGTTG.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

17bp deletion in the third common intron.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The tracheal dorsal trunk is long and tortuous in mutant embryos compared to wild type.

variMD109/vari03953b embryos die at the end of embryogenesis with convoluted tracheal tubes. Pleated septate junctions are missing between epidermal and between tracheal cells in stage 16 homozygous embryos, while adherens junctions are well developed.

vari48EP/vari03953b embryos have large dilations along the dorsal trunk.

Trachea are longer than normal in mutant embryos.

Mutants show defects in septate junction barrier function (as tested using a dye exclusion assay). The defects show variable penetrance and expressivity depending on the genetic background.

Unlike wild-type, post stage 15 trachea in vari03953b homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier.

Mutation has no obvious effect on the morphology of the salivary gland or hindgut. Early tracheal development, including primary branch budding and outgrowth and lumen formation and morphology occur normally during stages 11-14. Stage 16 embryos show a number of tracheal defects. These include an increased length of the dorsal trunk compared to wild type (with no accompanying increase in cell number), mild defects in the diameter of the dorsal trunk and moderate defects in the diameter of other primary branches. Irregular and variable tracheal tube morphologies, with local constrictions and/or dilations are seen in all major branches. The defects are first seen at stage 15.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Additional Comments
Genetic Interactions
Statement
Reference

The tracheal tube length defects caused by vari03953b are enhanced in the embryos are also mutant for pio2R-20.

The tracheal defects of sinunwu7 mutant embryos are more severe in double mutant combination with vari03953b (severe diameter defects are seen in all multicellular branches and dorsal trunk length appears increased).

The tracheal phenotypes of nrv2k04223 homozygotes are exacerbated by vari03953b/vari03953b.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Separable from: P{PZ}aop03953a. The vari03953b mutation is separable from the aop03953a mutation caused by the P{PZ}aop03953a insertion.

Comments
Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Lethality not caused by P{PZ}aop03953a.

Complements: dia2. Complements: dia5.

Complements: l(2)0023100231. Complements: l(2)0963909639. Complements: l(2)k09903k09903. Complements: l(2)k11038k11038. Complements: l(2)s4989s4989. Complements: l(2)s5211s5211. Complements: l(2)s5379s5379.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (15)