FlyBase Allele Report: Dmel\Sumo[04493]

General Information

Symbol

Dmel\Sumo⁰⁴⁴⁹³

Species

D. melanogaster

Name

FlyBase ID

FBal0008063

Feature type

allele

Associated gene

Dmel\Sumo

Associated Insertion(s)

P{PZ}Sumo⁰⁴⁴⁹³

Carried in Construct

Also Known As

smt3⁰⁴⁴⁹³, l(2)04493, smt3⁴⁴⁹³, P{PZ}smt3⁰⁴⁴⁹³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Sumo⁰⁴⁴⁹³

Cytology

Description

P{PZ} insertion about 10bp upstream of the first exon.

(Schnorr et al., 2001)

P{PZ} insertion 33bp upstream of the 5' end of the CT14617 transcript of smt3.

(Misra, 2000.8.25)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:6,967,613..6,967,613 [+]

(Saja et al., 2010, Sánchez et al., 2010, Chen et al., 2008, Schnorr et al., 2001, Misra, 2000.8.25, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage (with Sumo^k01211)

(Schnorr et al., 2001)

dorsal appendage | germline clone

(Nie et al., 2009)

egg (with Sumo^k01211)

(Schnorr et al., 2001)

egg chamber (with Sumo^k01211)

(Schnorr et al., 2001)

embryonic/first instar larval cuticle | germline clone

(Nie et al., 2009)

spindle | germline clone

(Nie et al., 2009)

Detailed Description

Statement

Reference

Less than 30% of smt3⁰⁴⁴⁹³ germ-line clone (GLC) embryos hatch and all hatched larvae die during the first larval instar. Greater than 90% of the unhatched embryos die prior to cuticle formation. The embryos that deposit cuticle exhibit a wide range of both anteroposterior and dorsoventral patterning defects. These defects are not rescued by zygotic smt3 expression as embryos produced by GLC females mated with wild type males show a comparable spectrum of phenotypes as those embryos produced by GLC females mated with heterozygous males.

Approximately 10% of smt3⁰⁴⁴⁹³ GLC eggs exhibit partially to fully fused dorsal appendages.

Over 50% of smt3⁰⁴⁴⁹³ GLC embryos exhibit a broad spectrum of nuclear cycle defects, including irregular size and distribution of nuclei, asynchronous nuclear division, abnormal interphase chromosome structure, overly condensed chromosomes, loss of sister chromatid cohesion during metaphase, polyploidy, chromosome clustering, fragmentation, and chromosome bridges. smt3⁰⁴⁴⁹³ GLC embryos often contain a reduced number of nuclei in relation to the centrosome pairs, and show defects in mitotic spindle organization and attachment to centrosomes and chromosomes.

(Nie et al., 2009)

Average Citrate Synthase activity is increased by 6% in heterozygous males

(Chen et al., 2008)

0.76% of smt3⁰⁴⁴⁹³/smt3^k01211 animals survive as adult escapers. These females produce few late-stage egg chambers and these rare eggs have poorly developed dorsal appendages.

(Schnorr et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Sumo⁰⁴⁴⁹³/smt3[+] is an enhancer of visible | dominant phenotype of tna¹

(Monribot-Villanueva et al., 2013)

Sumo⁰⁴⁴⁹³/smt3[+] is an enhancer of visible | dominant phenotype of osa¹

(Monribot-Villanueva et al., 2013)

Suppressor of

Statement

Reference

Sumo⁰⁴⁴⁹³/smt3[+] is a suppressor of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Other

Statement

Reference

Df(2L)32FP-5/+, Sumo⁰⁴⁴⁹³ has visible | dominant phenotype

(Sánchez et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Sumo⁰⁴⁴⁹³/smt3[+] is an enhancer of wing phenotype of tna¹

(Monribot-Villanueva et al., 2013)

Sumo⁰⁴⁴⁹³/smt3[+] is an enhancer of wing phenotype of osa¹

(Monribot-Villanueva et al., 2013)

lwr⁰⁵⁴⁸⁶/Sumo⁰⁴⁴⁹³ is an enhancer of wing margin phenotype of ct⁶, mod(mdg4)^ul

(Capelson and Corces, 2006)

Sumo⁰⁴⁴⁹³ is an enhancer of dorsal appendage phenotype of Ras85D^ix12a

(Schnorr et al., 2001)

Suppressor of

Statement

Reference

Sumo⁰⁴⁴⁹³/smt3[+] is a suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

lwr⁰⁵⁴⁸⁶/Sumo⁰⁴⁴⁹³ is a suppressor of adult cuticle phenotype of ct⁶, mod(mdg4)^ul

(Capelson and Corces, 2006)

lwr⁰⁵⁴⁸⁶/Sumo⁰⁴⁴⁹³ is a suppressor of adult cuticle phenotype of mod(mdg4)^ul, y²

(Capelson and Corces, 2006)

lwr⁰⁵⁴⁸⁶/Sumo⁰⁴⁴⁹³ is a suppressor of wing margin phenotype of ct⁶, mod(mdg4)^ul

(Capelson and Corces, 2006)

Other

Statement

Reference

Df(2L)32FP-5/+, Sumo⁰⁴⁴⁹³ has wing phenotype

(Sánchez et al., 2010)

Df(2L)32FP-5/+, Sumo⁰⁴⁴⁹³ has wing vein | ectopic phenotype

(Sánchez et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

smt3⁰⁴⁴⁹³/+ enhances both the penetrance and expressivity of the held-out wing phenotype of tna¹/+ flies. A maternal effect is observed in the enhancement of the tna¹/+ held out phenotype by smt3⁰⁴⁴⁹³/+.

smt3⁰⁴⁴⁹³/+ augments the penetrance and the expressivity of the weak held-out wing phenotype of osa¹/+.

(Monribot-Villanueva et al., 2013)

A smt3⁰⁴⁴⁹³/+ background suppresses the visual system degeneration seen in Scer\GAL4^GMR.PF, Ppt1^Scer\UAS.cKa flies.

(Saja et al., 2010)

smt3⁰⁴⁴⁹³/Df(2L)32FP-5 double heterozygotes have ectopic wing vein L3 material in the L2-L3 intervein region in 86% of cases. The wing size is reduced in size by 10% compared to wild type.

(Sánchez et al., 2010)

The transheterozygote lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ suppresses the mod(mdg4)^ul suppression of the ct⁶ jagged wing phenotype, leading to a more jagged and discontinuous wing phenotype. The phenotype is also particularly pronounced in females, although it is detectable in both sexes.

The transheterozygote lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ suppresses the mod(mdg4)^ul suppression of the y² cuticle phenotype, leading to a lighter coloration of the abdomen (due to a reduction in y expression).These phenotypic changes occur in approximately 30% of the mutant flies, with the remaining 70% displaying a continuum of less-pronounced effect. The phenotype is also particularly pronounced in females, although it is detectable in both sexes.

(Capelson and Corces, 2006)

Enhancer of the Ras85D^ix12a eggshell phenotype; 57% of eggs have fused and forked dorsal appendages.

(Schnorr et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

(Nie et al., 2009)

(Nie et al., 2009)

(Misra, 2000.8.25)

(BDGP Project Members, 1994-1999)

Sumo⁰⁴⁸⁴¹

(BDGP Project Members, 1994-1999)

Sumo^k01211

(BDGP Project Members, 1994-1999)

Sumo^k04501

(BDGP Project Members, 1994-1999)

Sumo^k06307

(BDGP Project Members, 1994-1999)

Sumo^k09930

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11378

P{PZ}Sumo⁰⁴⁴⁹³ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: snRNP70K⁰²¹⁰⁷. Complements: Hrb27C⁰²⁶⁴⁷. Complements: l(2)k00213^k00213. Complements: milt^k04704. Complements: Coprox^k10617. Complements: Hrb27C^rF680. Complements: poe⁰³⁴²⁰. Complements: cuc⁰⁶⁴⁹¹.

(BDGP Project Members, 1994-1999)

Embryos derived from smt3^k01211 and smt3⁰⁴⁴⁹³ germ-line clones (GLC) show an overlapping spectra of defects, with the average severity of defects being greater in smt3^k01211 GLC embryos than smt3⁰⁴⁴⁹³ GLC embryos.

(Nie et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Sumo⁰⁴⁴⁹³

(Ninova et al., 2023)

l(2)04493

(Schnorr and Moore, 2006, Bhaskar et al., 2002, Schnorr et al., 2001, Schnorr et al., 2001, Misra, 2000.8.25, Spradling et al., 1999)

l(2)04493⁰⁴⁴⁹³

(BDGP Project Members, 1994-1999)

smt3⁰⁴⁴⁹³

(Gao et al., 2024, Ng et al., 2024, Ninova et al., 2023, Tay and Pek, 2019, Monribot-Villanueva et al., 2013, Saja et al., 2010)

smt3⁴⁴⁹³

(Chen et al., 2008, Capelson and Corces, 2006)

sumo⁰⁴⁴⁹³

(Nie et al., 2009, Nie et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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