FlyBase Allele Report: Dmel\Sply[05091]

General Information

Symbol

Dmel\Sply⁰⁵⁰⁹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0008077

Feature type

allele

Associated gene

Dmel\Sply

Associated Insertion(s)

P{PZ}Sply⁰⁵⁰⁹¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Sply⁰⁵⁰⁹¹

Cytology

Description

P{PZ} insertion at position +269 relative to the start site of Sply EST LP04413.

(Herr et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:17,091,387..17,091,387 [+]

(Hull et al., 2024, Pantoja et al., 2013, Herr et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nephrotic syndrome

CEA

(Lovric et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Duchenne muscular dystrophy

modeled by Dys^RNAi.C.UAS

(Pantoja et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell

(Herr et al., 2003)

embryonic/larval garland cell | third instar larval stage (with Df(2R)BSC433)

(Lovric et al., 2017)

Detailed Description

Statement

Reference

Sply⁰⁵⁰⁹¹/Df(2R)BSC433 mutants display reduced viability and decreased foot process density in third instar larval garland cell nephrocytes accompanied by defects in the nephrocyte function.

(Lovric et al., 2017)

Sply⁰⁵⁰⁹¹ homozygous mutant adults present a small decrease in survival to starvation, but similar body weight, similar adiposity in abdominal sections, and similar sizes of fat body cells and lipid droplets, as compared to controls.

Sply⁰⁵⁰⁹¹ homozygous mutant adults present decreased feeding behavior, as assessed by decreased starvation-induced appetite, decreased postprandial meal volume, decreased average meal frequency and volume, and decreased daily food intake, as compared to controls.

(Walls et al., 2013)

Heterozygotes are indistinguishable from wild type. Homozygotes show 66.5% lethality. 46% lethality, compared with 3% in wild type, is seen during larval stages, with most larval death occurring during the first instar. 22% lethality, compared with 1% in wild type, is seen during metamorphosis. There is no difference in survival between wild-type and homozygous animals during embryogenesis. Mutant embryos show a pronounced enhancement of apoptosis compared with wild-type controls, especially in a specific region of the posterior pole near the developing genital disc. Muscles appear to develop normally in homozygous embryos and larvae. The dorsal oblique muscles (which serve as templates for the adult dorsal longitudinal muscles) appear normal in homozygous larvae. Homozygous females lay an average of 15.8 +/- 2.98 eggs per day, compared to 44.5 +/- 3.28 per day for wild-type females. The number of eggs produced is significantly reduced in crosses of both male and female mutant flies to wild-type mates. 91% of homozygous adults are flightless, scoring zero in a standard flight performance assay (the average flight score is 0.40 +/- 0.036 compared to 2.60 +/- 0.032 in wild-type flies). Homozygous flies show an accumulation of phosphorylated and unphosphorylated sphingolipids. Homozygous adults show a general pattern of missing dorsal longitudinal flight muscle (DLM) fibres, asymmetry and hypertrophy of remaining fibres. Muscle insertions are not affected. The average number of DLMs per hemithorax is 4.15 +/- 0.21 in the mutant flies, compared to 6.00 +/- 0.00 in wild-type flies. The persistent DLMs have intact myofibrils and normal mitochondria and nuclei. The function of the jump and leg muscles does not appear to be affected in homozygous flies. The giant fiber neuromuscular pathway appears functionally intact by electrophysiology analysis.

(Herr et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sply⁰⁵⁰⁹¹ has abnormal starvation stress response | adult stage phenotype, enhanceable by lace²/lace^k05305

(Walls et al., 2013)

Suppressed by

Statement

Reference

Sply⁰⁵⁰⁹¹ has increased cell death phenotype, suppressible by lace[+]/lace^k05305

(Herr et al., 2003)

Sply⁰⁵⁰⁹¹ has lethal | recessive phenotype, suppressible by lace[+]/lace^k05305

(Herr et al., 2003)

Sply⁰⁵⁰⁹¹ has flightless | recessive phenotype, suppressible by lace[+]/lace^k05305

(Herr et al., 2003)

Sply⁰⁵⁰⁹¹ has semi-sterile | recessive phenotype, suppressible by lace[+]/lace^k05305

(Herr et al., 2003)

NOT suppressed by

Statement

Reference

Sply⁰⁵⁰⁹¹ has abnormal feeding behavior | adult stage phenotype, non-suppressible by lace²/lace^k05305

(Walls et al., 2013)

Enhancer of

Statement

Reference

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is an enhancer of abnormal starvation stress response | adult stage phenotype of lace²/lace^k05305

(Walls et al., 2013)

Suppressor of

Statement

Reference

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is a suppressor of lethal | recessive phenotype of lace^k05305

(Herr et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sply⁰⁵⁰⁹¹ has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by lace[+]/lace^k05305

(Herr et al., 2003)

NOT Enhancer of

Statement

Reference

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is a non-enhancer of adult fat body phenotype of lace²/lace^k05305

(Walls et al., 2013)

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is a non-enhancer of lipid droplet | adult stage phenotype of lace²/lace^k05305

(Walls et al., 2013)

Suppressor of

Statement

Reference

Sply⁰⁵⁰⁹¹/Sply[+] is a suppressor of indirect flight muscle cell phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Pantoja et al., 2013)

Sply⁰⁵⁰⁹¹/Sply[+] is a suppressor of myofibril phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Pantoja et al., 2013)

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is a suppressor of wing phenotype of lace^k05305

(Herr et al., 2003)

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is a suppressor of chaeta phenotype of lace^k05305

(Herr et al., 2003)

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is a suppressor of eye phenotype of lace^k05305

(Herr et al., 2003)

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ is a suppressor of ommatidium phenotype of lace^k05305

(Herr et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Sply⁰⁵⁰⁹¹ suppresses the defects in indirect flight muscle myofibril integrity seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU. This suppression is further enhanced when the flies are given food supplemented with 2-acetyl-4(5)-tetrahydroxybutyl imidazole (THI).

One copy of Sply⁰⁵⁰⁹¹ suppresses the defects in indirect flight muscle myofibril integrity seen in Df(3R)Exel6184 mutant flies. The gross defects in indirect flight muscle morphology are also suppressed.

(Pantoja et al., 2013)

lace^k05305/lace², Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ adults exhibit a more severe decrease in survival in response to starvation as compared to either lace^k05305/lace² or Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ mutants.

The decreased body weight, decreased adiposity in abdominal sections, and decreased sizes of fat body cells and lipid droplets, observed in lace^k05305/lace² transheterozygous mutant adults is not altered by Sply⁰⁵⁰⁹¹ homozygosity.

The decreased feeding behavior observed in Sply⁰⁵⁰⁹¹ homozygous mutant adults, as assessed by their decreased starvation-induced appetite, decreased postprandial meal volume, decreased average meal frequency and volume, and decreased daily food intake, is not suppressed by lace^k05305/lace² transheterozygosity.

(Walls et al., 2013)

Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ lace^k05305/+ adults have an average flight score of 1.41 +/- 0.063 compared to 2.60 +/- 0.032 in wild-type flies in a standard flight performance assay. The average number of dorsal longitudinal flight muscles per hemithorax is 5.13 +/- 0.26 in the mutant flies, compared to 6.00 +/- 0.00 in wild-type flies. Sply⁰⁵⁰⁹¹ lace^k05305 double homozygotes show 61% lethality. The eye, bristle and wing defects of lace^k05305 surviving adults are suppressed in Sply⁰⁵⁰⁹¹ lace^k05305 double homozygotes.

(Herr et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2R)BSC433/Sply⁰⁵⁰⁹¹ is rescued by Sply^WT.Tag:HA

(Lovric et al., 2017)

Df(2R)BSC433/Sply⁰⁵⁰⁹¹ is rescued by Sply^E119G.Tag:HA

(Lovric et al., 2017)

Not rescued by

Df(2R)BSC433/Sply⁰⁵⁰⁹¹ is not rescued by Sply^R210Q.Tag:HA

(Lovric et al., 2017)

Df(2R)BSC433/Sply⁰⁵⁰⁹¹ is not rescued by Sply^S335I.Tag:HA

(Lovric et al., 2017)

Comments

The reduced density of foot process in garland cell nephrocytes observed in Sply⁰⁵⁰⁹¹/Df(2R)BSC433 third instar larvae can be rescued by combination with either Sply^{WT.T:Ivir\HA1} or Sply^{E119G.T:Ivir\HA1} but not Sply^{R210Q.T:Ivir\HA1} or Sply^{S335I.T:Ivir\HA1}.

(Lovric et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11393

cn¹ P{PZ}Sply⁰⁵⁰⁹¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: GstS1⁰⁴²²⁷. Complements: RhoGEF2⁰⁴²⁹¹. Complements: l(2)06253⁰⁶²⁵³. Complements: l(2)06655⁰⁶⁶⁵⁵. Complements: GstS1^k08805. Complements: l(2)k09303^k09303. Complements: l(2)k09854^k09854. Complements: l(2)k11301^k11301. Complements: l(2)k11405^k11405.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Sply(05091)

(Chaturvedi et al., 2019)

Sply⁰⁵⁰⁹¹

(Hull et al., 2024, Lovric et al., 2017, Pantoja et al., 2013, Walls et al., 2013)

l(2)05091

(Spradling et al., 1999)

l(2)05091⁰⁵⁰⁹¹

(BDGP Project Members, 1994-1999)

sply⁰⁵⁰⁹¹

(Chaturvedi et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (10)

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