Allele Dmel\lwr⁰⁵⁴⁸⁶

General Information
Symbol	Dmel\lwr⁰⁵⁴⁸⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0008090
Feature type	allele	Associated gene	Dmel\lwr
Also Known As	l(2)05486, lwr⁵⁴⁸⁶

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele, hypomorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Capelson and Corces, 2006) loss of function allele (Apionishev and Rasooly, 1997)
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2L:542,248..542,560 2L:542,481..542,481 (BDGP Project Members, 1994-1999, Watts et al., 2003, Spradling et al., 1999, Apionishev et al., 2001, Sánchez et al., 2010, Pardi et al., 2011)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion in the 5' untranslated region. (Apionishev et al., 2001) P{PZ} is inserted into the 5' UTR of lwr. (Epps and Tanda, 1998)
Caused by insertion	P{PZ}lwr⁰⁵⁴⁸⁶ (BDGP Project Members, 1994-1999, Watts et al., 2003, Spradling et al., 1999, Apionishev et al., 2001, Sánchez et al., 2010, Pardi et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Apionishev et al., 2001, Spradling et al., 1999, Apionishev and Rasooly, 1997, Bhaskar et al., 2002) neuroanatomy defective \| somatic clone (Watts et al., 2003)
Phenotype Manifest In
	adult head (with lwr^unspecified) (Epps and Tanda, 1998) embryonic epidermis (Epps and Tanda, 1998) eye (with lwr^unspecified) (Epps and Tanda, 1998) leg (with lwr^unspecified) (Epps and Tanda, 1998) neuroblast \| somatic clone (Watts et al., 2003) wing (with lwr^unspecified) (Epps and Tanda, 1998)
Detailed Description
	Statement Reference No defects are seen in axon pruning during metamorphosis, though a severe proliferation defect is seen when somatic clones are made in the neuroblast. (Watts et al., 2003) Embryos transheterozygous for lwr⁰⁵⁴⁸⁶ and lwr⁰²⁸⁵⁸ show cuticle patterns resembling those of lwr¹¹⁸ homozygotes and of lwr⁰⁵⁴⁸⁶/lwr¹¹⁸ transheterozygotes: a phenotype intermediate between the bcd and the hb mutant phenotypes. bcd protein is retained in the cytoplasm of lwr⁰⁵⁴⁸⁶/lwr⁰²⁸⁵⁸ transheterozygotes and lwr⁰⁵⁴⁸⁶ homozygotes. (Epps and Tanda, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference lwr⁰⁵⁴⁸⁶/lwr[+] is an enhancer of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, bchs^EP2299 (Simonsen et al., 2007)
Suppressor of
Statement Reference lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor \| partially of meiotic cell cycle defective \| female phenotype of mei-218⁶ (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor of meiotic cell cycle defective \| dominant \| female phenotype of nod^DTW (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor of meiotic cell cycle defective \| female phenotype of ncd^D/ncd¹ (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor of meiotic cell cycle defective \| female phenotype of nod²/nod^a (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶ is a suppressor \| partially of meiotic cell cycle defective \| female phenotype of Axs^D (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶ is a suppressor of meiotic cell cycle defective \| female phenotype of mei-218¹, nod^DTW (Apionishev et al., 2001)
Other
Statement Reference Df(2L)32FP-5/+, lwr⁰⁵⁴⁸⁶ has visible \| dominant phenotype (Sánchez et al., 2010)
Phenotype Manifest In
Enhancer of
Statement Reference lwr⁰⁵⁴⁸⁶/lwr[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, bchs^EP2299 (Simonsen et al., 2007) lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ is an enhancer of wing margin phenotype of ct⁶, mod(mdg4)^ul (Capelson and Corces, 2006)
NOT Enhancer of
Statement Reference lwr⁰⁵⁴⁸⁶, smt3^k06307, smt3[+], lwr[+] is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, p53^Scer\UAS.Ex (Pardi et al., 2011) lwr⁰⁵⁴⁸⁶/lwr[+] is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, p53^Scer\UAS.Ex (Pardi et al., 2011) lwr⁰⁵⁴⁸⁶ is a non-enhancer of wing vein \| ectopic phenotype of Cbl^Dv.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Robertson et al., 2000)
Suppressor of
Statement Reference lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor \| partially of meiosis & nuclear chromosome \| female phenotype of mei-218⁶ (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor of meiosis & nuclear chromosome \| female phenotype of ncd^D/ncd¹ (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor of meiosis & nuclear chromosome \| female phenotype of nod²/nod^a (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶/lwr[+] is a suppressor of meiosis & nuclear chromosome \| female phenotype of nod^DTW (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ is a suppressor of adult cuticle phenotype of ct⁶, mod(mdg4)^ul (Capelson and Corces, 2006) lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ is a suppressor of adult cuticle phenotype of mod(mdg4)^ul, y² (Capelson and Corces, 2006) lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ is a suppressor of wing margin phenotype of ct⁶, mod(mdg4)^ul (Capelson and Corces, 2006) lwr⁰⁵⁴⁸⁶ is a suppressor \| partially of meiosis & nuclear chromosome \| female phenotype of Axs^D (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶ is a suppressor of meiosis & nuclear chromosome \| female phenotype of mei-218¹, nod^DTW (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶ is a suppressor of oocyte & spindle phenotype of mei-218¹, nod^DTW (Apionishev et al., 2001) lwr⁰⁵⁴⁸⁶ is a suppressor of phenotype of nod^DTW (Apionishev and Rasooly, 1997)
NOT Suppressor of
Statement Reference lwr⁰⁵⁴⁸⁶, smt3^k06307, smt3[+], lwr[+] is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, p53^Scer\UAS.Ex (Pardi et al., 2011) lwr⁰⁵⁴⁸⁶/lwr[+] is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, p53^Scer\UAS.Ex (Pardi et al., 2011) lwr⁰⁵⁴⁸⁶ is a non-suppressor of wing vein \| ectopic phenotype of Cbl^Dv.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Robertson et al., 2000)
Other
Statement Reference Df(2L)32FP-5/+, lwr⁰⁵⁴⁸⁶ has wing phenotype (Sánchez et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference A loss of heterozygosity assay, using mwh[1] as a marker, reveals that a lwr[05486] heterozygous background increases the number of mwh[1] clones after irradiation with 250 rad of X-rays, in p53[5A-1-4] mutants. The rough eye phenotype found at 29[o]C in flies expressing p53[Scer\UAS.Ex] under the control of Scer\GAL4[GMR.PF] is not affected by a lwr[05486] heterozygous background nor in a lwr[05486]-smt3[k06307] transheterozygous background. (Pardi et al., 2011) lwr[05486]/Df(2L)32FP-5 double heterozygotes show a reduction in wing size (by 10%) compared to wild type. (Sánchez et al., 2010) The transheterozygote lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ suppresses the mod(mdg4)^ul suppression of the ct⁶ jagged wing phenotype, leading to a more jagged and discontinuous wing phenotype. The phenotype is also particularly pronounced in females, although it is detectable in both sexes. The transheterozygote lwr⁰⁵⁴⁸⁶/smt3⁰⁴⁴⁹³ suppresses the mod(mdg4)^ul suppression of the y² cuticle phenotype, leading to a lighter coloration of the abdomen (due to a reduction in y expression).These phenotypic changes occur in approximately 30% of the mutant flies, with the remaining 70% displaying a continuum of less-pronounced effect. The phenotype is also particularly pronounced in females, although it is detectable in both sexes. (Capelson and Corces, 2006) lwr⁰⁵⁴⁸⁶ dominantly suppresses the nondisjunction phenotype of nod^DTW/+ females. nod^a/FM7a nod² female nondisjunction is dominantly suppressed by lwr⁰⁵⁴⁸⁶. 4th, but not X chromosome disjunction is suppressed in mei-218⁶ females if they also carry lwr⁰⁵⁴⁸⁶/+. 4th, but not X chromosome disjunction is suppressed in Axs^D females if they also carry lwr⁰⁵⁴⁸⁶. ncd¹/ncd^D female X and 4th chromosome nondisjunction is dominantly suppressed by lwr⁰⁵⁴⁸⁶. nod^DTW mei-218¹ double homozygous females show 60% X chromosome nondisjunction and 63% 4th chromosome nondisjunction. Oocyte spindles are often frayed or oddly shaped, or multiple spindles are present. These defects are suppressed by lwr⁰⁵⁴⁸⁶/+. (Apionishev et al., 2001) A lwr[05486] mutant background does not affect the extent of ectopic wing veins or eye roughening seen in Scer\GAL4[GMR.PF] Cbl[Dv.Scer\UAS] mutants. (Robertson et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	lwr⁰²⁸⁵⁸ (Apionishev et al., 2001, BDGP Project Members, 1994-1999) lwr^semi710 (Apionishev et al., 2001)
Partially complements	lwr⁰²⁸⁵⁸ (Epps and Tanda, 1998, Epps and Tanda, 1998) lwr^unspecified (Epps and Tanda, 1998)
Fails to complement	lwr⁵ (Apionishev et al., 2001) lwr^01519a (BDGP Project Members, 1994-1999)
Rescued by	lwr⁰⁵⁴⁸⁶ is rescued by lwr^+t2.4 (Casso et al., 2005)
Comments	Percent hatchability of embryos produced from a cross between lwr⁰⁵⁴⁸⁶ and lwr⁰²⁸⁵⁸ is 75%, whereas hatchability from a cross between ush² and lwr⁰²⁸⁵⁸ is 90%. (Epps and Tanda, 1998)
Stocks ( 1 )
Bloomington	11410 P{PZ}lwr⁰⁵⁴⁸⁶ cn¹/CyO; ry⁵⁰⁶
Notes on Origin
Discoverer	A. Spradling.

Comments
	Excision of the P{PZ} element can revert the mutant phenotype. (Apionishev et al., 2001) Most excisions of the P{PZ} element are homozygous viable and no longer suppress nod^DTW. Lethal excision alleles remain suppressors. (Apionishev and Rasooly, 1997) Complements: ex⁰¹²⁷⁰. Complements: ds⁰⁵¹⁴². Complements: Gsc⁰⁵³⁴¹. Complements: α-Adaptin⁰⁶⁶⁹⁴. Complements: S^k06101. Complements: l(2)k08915^k08915. Complements: l(2)k09610^k09610. Complements: Nle^k13714. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	l(2)05486 (Bhaskar et al., 2002, Apionishev et al., 2001, Spradling et al., 1999, Epps and Tanda, 1998, Epps and Tanda, 1998) l(2)05486⁰⁵⁴⁸⁶ lesswright^r05486 (Simonsen et al., 2007) lwr⁵⁴⁸⁶ (Apionishev et al., 2001, Capelson and Corces, 2006) lwr⁰⁵⁴⁸⁶ (Robertson et al., 2000) unnamed (Apionishev and Rasooly, 1997)
Name Synonym
Secondary FlyBase IDs

References ( 17 )
Research paper	Pardi et al., 2011, Acta biol. hung. 62(4): 397--412 In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53. [FBrf0216737] Sánchez et al., 2010, J. Biol. Chem. 285(33): 25841--25849 Sumoylation Modulates the Activity of Spalt-like Proteins during Wing Development in Drosophila. [FBrf0211449] Simonsen et al., 2007, Genetics 176(2): 1283--1297 Genetic modifiers of the Drosophila blue cheese gene link defects in lysosomal transport with decreased life span and altered ubiquitinated-protein profiles. [FBrf0201586] Capelson and Corces, 2006, EMBO J. 25(9): 1906--1914 SUMO conjugation attenuates the activity of the gypsy chromatin insulator. [FBrf0193462] Casso et al., 2005, Genetics 170(1): 139--148 Drosophila signal peptide peptidase is an essential protease for larval development. [FBrf0188526] Watts et al., 2003, Neuron 38(6): 871--885 Axon pruning during Drosophila metamorphosis: evidence for local degeneration and requirement of the ubiquitin-proteasome system. [FBrf0161044] Bhaskar et al., 2002, Mol. Cell. Biol. 22(2): 492--504 Conjugation of smt3 to dorsal may potentiate the Drosophila immune response. [FBrf0141657] Apionishev et al., 2001, Genes Cells 6(3): 215--224 The Drosophila UBC9 homologue lesswright mediates the disjunction of homologues in meiosis I. [FBrf0134637] Robertson et al., 2000, Oncogene 19(29): 3299--3308 A Drosophila analogue of v-Cbl is a dominant-negative oncoprotein in vivo. [FBrf0130045] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Epps and Tanda, 1998, Curr. Biol. 8(23): 1277--1280 The Drosophila semushi mutation blocks nuclear import of bicoid during embryogenesis. [FBrf0105226]
Supplementary material	Epps and Tanda, 1998, Curr. Biol. 8(23): The Drosophila semushi mutation blocks nuclear import of bicoid during embryogenesis. [FBrf0107698]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Rasooly, 1997.4.18, [title not yet available] [title not yet available] [FBrf0093832] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Apionishev and Rasooly, 1997, A. Dros. Res. Conf. 38: 171A Genetic characterization of the suppressing activities of lesswright, a dominant modifier of nod^DTW. [FBrf0092090]

Allele Dmel\lwr05486

Allele Dmel\lwr⁰⁵⁴⁸⁶