Allele Dmel\Acsl⁰⁵⁸⁴⁷

General Information
Symbol	Dmel\Acsl⁰⁵⁸⁴⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0008097
Feature type	allele	Associated gene	Dmel\Acsl
Also Known As	l(2)05847, dAcsl⁰⁵⁸⁴⁷

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:4,556,341..4,556,341 (BDGP Project Members, 1994-1999, Misra, 2000.11.18, Spradling et al., 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion in intron 1 of the CT25221 transcript of l(2)44DEa, 3089bp downstream of the 5' end of the transcript, 473bp downstream of the end of exon 1. P{PZ} element is inserted in the same orientation as the gene. (Misra, 2000.11.18)
Caused by insertion	P{PZ}Acsl⁰⁵⁸⁴⁷ (BDGP Project Members, 1994-1999, Misra, 2000.11.18, Spradling et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Acsl⁸) (Zhang et al., 2009) lethal \| larval stage \| recessive (Amorim et al., 1995) lethal \| pharate adult stage \| recessive (Liu et al., 2011) lethal \| recessive (Spradling et al., 1999, Zhang et al., 2009) mitotic cell cycle defective \| recessive (Amorim et al., 1995) neuroanatomy defective \| larval stage (with Acsl^KO) (Liu et al., 2011) neurophysiology defective (with Acsl^KO) (Liu et al., 2011)
Phenotype Manifest In
	bouton (with Acsl^KO) (Liu et al., 2011) embryonic/larval neuromuscular junction (with Acsl^KO) (Liu et al., 2011) larval abdominal segment 6 (with Acsl^KO) (Liu et al., 2011) larval abdominal segment 7 (with Acsl^KO) (Liu et al., 2011) segmental nerve (with Acsl^KO) (Liu et al., 2011) synapse (with Acsl^KO) (Liu et al., 2011)
Detailed Description
	Statement Reference Acsl[05847] mutant survive to the pharate adult stage. In the cross section of the larval segmental nerves of Acsl[KO]/Acsl[05847] mutants, there are conspicuous axonal swellings packed with heterogeneous membranous organelles. These aggregates, most often observed in posterior axons, include dark, prelysosomal and multivesicular bodies. In some mutant larvae, autophagosomes are also observed where a cluster of large clear-core vesicles are surrounded by a double-layer membrane. The mutant nerves also display lamellated bodies which resemble autolysosomes. In other cases, clusters of large clear-core vesicles are observed. These membrane aggregates are rarely found in wild-type axons. Acsl[KO]/Acsl[05847] mutants display pronounced dystrophy of larval neuromuscular junction 4 (NMJ4) in the posterior abdominal segments A6 and A7, while the muscle and larval size are comparable to wild-type. The bouton number and the synaptic area are significantly reduced in Acsl[KO]/Acsl[05847] mutants compared with wild-type. The synaptic area of Acsl[KO]/Acsl[05847] in Acsl[KO]/Acsl[05847] mutants is comparable to that of wild-types 2 days after egg laying (AEL). Synapse growth during 2-3.5 days AEL of Acsl[KO]/Acsl[05847] mutants is markedly slower than that in wild-types. From 3.5 to 5 days AEL, the mutant NMJ synapses apparently retract. The evoked excitatory junction potentials (EJPs) are significantly reduced in Acsl[KO]/Acsl[05847] mutant larvae recorded from muscle 6 in the posterior abdominal segment. Miniature EJPs (mEJPs, also known as quantal size) are normal in Acsl[KO]/Acsl[05847] mutants. Quantal content is significantly reduced in Acsl[KO]/Acsl[05847] mutants. (Liu et al., 2011) Neutral lipid accumulation is blocked in Acsl[8]/Acsl[05847] wing discs. Total triacylglycerol content of Acsl[8]/Acsl[05847] pharates is significantly lower than wild type. (Zhang et al., 2009) Neuroblast squashes reveal premature separation of sister chromatids during prophase and metaphase, overcondensed chromosomes in metaphase and anaphase, and anaphases with different degrees of abnormalities. Spindles are abnormal, some being of abnormal shape and others failing to connect all the chromosomes. (Amorim et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, suppressible \| partially by Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, suppressible \| partially by Scer\GAL4^tub.PU/Hsap\ACSL3^{Scer\UAS.T:Hsap\MYC} (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl^KO has neuroanatomy defective \| larval stage phenotype, suppressible by Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^αTub84B.PL (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has neurophysiology defective phenotype, suppressible \| partially by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has neurophysiology defective phenotype, suppressible by Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^αTub84B.PL (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has neurophysiology defective phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011)
NOT suppressed by
Statement Reference Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, non-suppressible by Hsap\ACSL4^{P375L.Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, non-suppressible by Scer\GAL4^tub.PU/Hsap\ACSL4^{R570S.Scer\UAS.L.T:Hsap\MYC} (Zhang et al., 2009)
Suppressor of
Statement Reference Acsl⁰⁵⁸⁴⁷/l(2)44DEa[+] is a suppressor of visible \| heat sensitive phenotype of peb¹ (Wilk et al., 2004)
Phenotype Manifest In
Suppressed by
Statement Reference Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, suppressible by Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, suppressible by Hsap\ACSL4^{Scer\UAS.S.T:Hsap\MYC}/Scer\GAL4^tub.PU (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, suppressible by Scer\GAL4^tub.PU/Hsap\ACSL3^{Scer\UAS.T:Hsap\MYC} (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl^KO has bouton phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has synapse phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011)
NOT suppressed by
Statement Reference Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, non-suppressible by Hsap\ACSL4^{P375L.Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, non-suppressible by Scer\GAL4^tub.PU/Hsap\ACSL4^{R570S.Scer\UAS.L.T:Hsap\MYC} (Zhang et al., 2009) Acsl⁰⁵⁸⁴⁷/Acsl^KO has bouton phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011)
Suppressor of
Statement Reference Acsl⁰⁵⁸⁴⁷/l(2)44DEa[+] is a suppressor of eye \| heat sensitive phenotype of peb¹ (Wilk et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] in postsynaptic muscles driven by Scer\GAL4[Mhc.PW] does not suppress the dystrophic neuromuscular junctions of Acsl[KO]/Acsl[05847] mutants for bouton number. Neuronal expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] at 2.5 days after egg laying (AEL) fully suppresses the reduced synaptic area phenotype of Acsl[KO]/Acsl[05847] mutants. Neuronal expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] driven by Scer\GAL4[elav.PU] restores the bouton number to wild-type levels in Acsl[KO]/Acsl[05847] mutants. The neuromuscular junction phenotypes of Acsl[KO]/Acsl[05847] mutants can be fully suppressed by ubiquitous expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[αTub84B.PL]. The reduced EJPs and quantal content are fully rescued by Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] expressed in either ubiquitously via Scer\GAL4[αTub84B.PL] or pan-neuronally via Scer\GAL4[elav.PU]. EJPs in animals with Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] expressed pan-neuronally are mildly but significantly higher than that in wild-types. Postsynaptic expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] driven by Scer\GAL4[Mhc.PW] in Acsl[KO]/Acsl[05847] mutant background slightly suppressed the EJP phenotype. (Liu et al., 2011) Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] or Hsap\ACSL3[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[tub] partially rescues the lethality associated with the Acsl[8]/Acsl[05847] genotype. Expression of Hsap\ACSL4[R570S.Scer\UAS.L.T:Hsap\MYC] or Hsap\ACSL4[P375L.Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[tub] fails to rescue the lethality associated with the Acsl[8]/Acsl[05847] genotype. Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC], Hsap\ACSL4[Scer\UAS.S.T:Hsap\MYC] or Hsap\ACSL3[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[tub] restores neutral lipid staining in Acsl[8]/Acsl[05847] wing discs. Expression of Hsap\ACSL4[R570S.Scer\UAS.L.T:Hsap\MYC] or Hsap\ACSL4[P375L.Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[tub] fails to restore neutral lipid staining in Acsl[8]/Acsl[05847] wing discs. Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[tub] restores normal triacylglycerol levels in Acsl[8]/Acsl[05847] wing discs. (Zhang et al., 2009)
Complementation & Rescue Data
Fails to complement	Acsl⁰⁷⁴⁴⁷ (BDGP Project Members, 1994-1999) Acsl^k05512 (BDGP Project Members, 1994-1999) Acsl^k06526 (BDGP Project Members, 1994-1999) Acsl^k10313 (BDGP Project Members, 1994-1999) Acsl^k16116 (BDGP Project Members, 1994-1999)
Rescued by	Acsl⁰⁵⁸⁴⁷/Acsl⁸ is rescued by Acsl^{Scer\UAS.715.C.T:Hsap\MYC}/Scer\GAL4^tub.PU (Zhang et al., 2009)
Partially rescued by	Acsl⁰⁵⁸⁴⁷/Acsl⁸ is partially rescued by Acsl^{Scer\UAS.715.C.T:Hsap\MYC}/Scer\GAL4^tub.PU (Zhang et al., 2009)
Comments	Expression of Acsl[Scer\UAS.715.T:Hsap\MYC.C] under the control of Scer\GAL4[tub] partially rescues the lethality associated with the Acsl[8]/Acsl[05847] genotype. Expression of Acsl[Scer\UAS.715.T:Hsap\MYC.C] under the control of Scer\GAL4[tub] restores neutral lipid staining in Acsl[8]/Acsl[05847] wing discs. (Zhang et al., 2009)
Stocks ( 2 )
Bloomington	11452 cn¹ P{PZ}Acsl⁰⁵⁸⁴⁷/CyO; ry⁵⁰⁶ 32330 w^*; P{PZ}Acsl⁰⁵⁸⁴⁷/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7; P{UAS-Acsl.715.MycC}3/TM6B, Tb¹
Notes on Origin
Discoverer	A. Spradling.

Comments
	The P{EP}l(2)44DEa^EP2365 insertion is 286bp downstream of the P{PZ}l(2)44DEa⁰⁵⁸⁴⁷ insertion. (Misra, 2000.11.18) Complements: l(2)03996⁰³⁹⁹⁶. Complements: Rho1^k02107b. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	Acsl⁰⁵⁸⁴⁷ dAcsl⁰⁵⁸⁴⁷ (Zhang et al., 2009, Liu et al., 2011) l(2)44DEa⁰⁵⁸⁴⁷ l(2)05847 (Misra, 2000.11.18, Dockendorff et al., 2000, Spradling et al., 1999) l(2)05847⁰⁵⁸⁴⁷ P1452 (Amorim et al., 1995)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Zhang et al., 2009, Hum. Mol. Genet. 18(20): 3894--3905 Analyses of mental dysfunction-related ACSl4 in Drosophila reveal its requirement for Dpp/BMP production and visual wiring in the brain. [FBrf0209077] Wilk et al., 2004, Genetics 168(1): 281--300 Dose-sensitive autosomal modifiers identify candidate genes for tissue autonomous and tissue nonautonomous regulation by the Drosophila nuclear zinc-finger protein, hindsight. [FBrf0180294] Dockendorff et al., 2000, Mol. Gen. Genet. 263(1): 137--143 Genetic characterization of the 44D-45B region of the Drosophila melanogaster genome based on an F₂ lethal screen. [FBrf0126727] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Personal communication to FlyBase	Misra, 2000.11.18, nc5 report, third installment. nc5 report, third installment. [FBrf0132131] Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Amorim et al., 1995, Europ. Dros. Res. Conf. 14: 246 Characterization of a new mitotic mutant of Drosophila melanogaster. [FBrf0083005]

Allele Dmel\Acsl05847

Allele Dmel\Acsl⁰⁵⁸⁴⁷