FlyBase Allele Report: Dmel\cg[07659]

General Information

Symbol

Dmel\cg⁰⁷⁶⁵⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0008129

Feature type

allele

Associated gene

Dmel\cg

Associated Insertion(s)

P{PZ}cg⁰⁷⁶⁵⁹

Carried in Construct

Also Known As

l(2)07659, cg²

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}cg⁰⁷⁶⁵⁹

Cytology

Description

P{PZ} insertion in an intron in the 5' UTR.

(Campbell and Tomlinson, 2000)

P{PZ} insertion in intron 1.

(Svendsen et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Svendsen et al., 2000)

partially lethal - majority die

visible (with cg¹)

(Campbell and Tomlinson, 2000, Svendsen et al., 2000)

Phenotype Manifest In

leg disc (with cg¹)

(Svendsen et al., 2000)

leg disc anterior compartment

(Campbell and Tomlinson, 2000)

leg disc anterior compartment (with cg³)

(Svendsen et al., 2000)

prothoracic tibial preapical bristle | increased number (with cg³)

(Svendsen et al., 2000)

prothoracic tibial transverse row bristle | increased number (with cg¹)

(Svendsen et al., 2000)

sex comb | increased number (with cg¹)

(Campbell and Tomlinson, 2000, Svendsen et al., 2000)

sex comb | increased number (with cg³)

(Svendsen et al., 2000)

wing blade anterior compartment (with cg³)

(Svendsen et al., 2000)

wing blade posterior compartment (with cg¹)

(Svendsen et al., 2000)

wing margin | anterior (with cg¹)

(Svendsen et al., 2000)

wing vein L2 (with cg¹)

(Svendsen et al., 2000)

wing vein L4 (with cg¹)

(Campbell and Tomlinson, 2000, Svendsen et al., 2000)

wing vein L5 (with cg¹)

(Svendsen et al., 2000)

Detailed Description

Statement

Reference

Homozygous leg discs are oval with overgrowth in the anterior compartment. cg¹/cg⁰⁷⁶⁵⁹ flies have gaps in wing vein L4. cg¹/cg⁰⁷⁶⁵⁹ males have extra sex comb teeth.

(Campbell and Tomlinson, 2000)

Very few homozygotes survive to the third larval instar stage. Approximately 10% of cg¹/cg⁰⁷⁶⁵⁹ animals eclose to adult stages, with most of the remaining animals dying as pharate adults. cg¹/cg⁰⁷⁶⁵⁹ flies have defects in some combination of the posterior compartment longitudinal veins L4 and L5. Defects in vein L4, ranging from a slight thinning of the vein to gaps of increasing size are seen in 100% of wings. Defects in wing vein L5 are seen in less than 25% of flies. 40% of cg¹/cg⁰⁷⁶⁵⁹ flies have truncations of wing vein L2. Rarely (less than 1%), outgrowths are seen in the costal region of the anterior wing margin. cg³/cg⁰⁷⁶⁵⁹ animals survive to the pharate adult stage but never eclose. cg³/cg⁰⁷⁶⁵⁹ males have highly disorganised sex combs with as much as a threefold increase in the number of sex comb teeth. The pre-apical bristle may also be duplicated. cg³/cg⁰⁷⁶⁵⁹ legs discs show a substantial increase in the relative size of the anterior compartment. cg¹/cg⁰⁷⁶⁵⁹ leg discs show overgrowth in the anterior/posterior axis. cg¹/cg⁰⁷⁶⁵⁹ flies have expanded transverse rows and sex combs.

(Svendsen et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

cg[+]/cg⁰⁷⁶⁵⁹ is an enhancer of visible phenotype of en⁵⁹

(Campbell and Tomlinson, 2000)

cg[+]/cg⁰⁷⁶⁵⁹ is an enhancer of visible | dominant phenotype of ci^W

(Campbell and Tomlinson, 2000, Svendsen et al., 2000)

Suppressor of

Statement

Reference

cg[+]/cg⁰⁷⁶⁵⁹ is a suppressor of visible | somatic clone phenotype of wg^Act5C.PS

(McClure and Schubiger, 2008)

Other

Statement

Reference

Df(2R)en-11/+, cg⁰⁷⁶⁵⁹ has visible | dominant phenotype

(Svendsen et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

cg⁰⁷⁶⁵⁹/cg¹ has prothoracic tibial transverse row bristle | increased number phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/ci^UAS.Tag:HA

(Svendsen et al., 2000)

cg⁰⁷⁶⁵⁹/cg¹ has sex comb | increased number phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/ci^UAS.Tag:HA

(Svendsen et al., 2000)

cg⁰⁷⁶⁵⁹/cg¹ has leg disc phenotype, suppressible by Scer\GAL4^ptc-559.1/ci^UAS.Tag:HA

(Svendsen et al., 2000)

NOT suppressed by

Statement

Reference

cg⁰⁷⁶⁵⁹ has leg disc phenotype, non-suppressible by hh⁴

(Campbell and Tomlinson, 2000)

Enhancer of

Statement

Reference

cg[+]/cg⁰⁷⁶⁵⁹ is an enhancer of wing vein L4 phenotype of en⁵⁹

(Campbell and Tomlinson, 2000)

cg[+]/cg⁰⁷⁶⁵⁹ is an enhancer of wing vein L4 phenotype of ci^W

(Campbell and Tomlinson, 2000, Svendsen et al., 2000)

Suppressor of

Statement

Reference

cg[+]/cg⁰⁷⁶⁵⁹ is a suppressor of leg | somatic clone phenotype of wg^Act5C.PS

(McClure and Schubiger, 2008)

cg[+]/cg⁰⁷⁶⁵⁹ is a suppressor of wing | ectopic | somatic clone phenotype of wg^Act5C.PS

(McClure and Schubiger, 2008)

Other

Statement

Reference

Df(2R)en-11/+, cg⁰⁷⁶⁵⁹ has wing vein L4 phenotype

(Svendsen et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of leg-to-wing transdetermination caused by expression of wg^Act5C.PS in clones during the mid-second larval instar is dominantly suppressed by cg⁰⁷⁶⁵⁹.

(McClure and Schubiger, 2008)

hh⁴ has no effect on the cg⁰⁷⁶⁵⁹ leg disc phenotype (when the animals are raised at the restrictive temperature for hh⁴ during the 2nd and 3rd instar stages). The ci^W/+ wing vein L4 phenotype is enhanced by one copy of cg⁰⁷⁶⁵⁹. 25% of en⁵⁹ cg⁰⁷⁶⁵⁹ double heterozygotes have gaps in wing vein L4.

(Campbell and Tomlinson, 2000)

Df(2R)en-11/+ ; cg⁰⁷⁶⁵⁹/+ double heterozygotes have a gap in wing vein L4. The wing vein L4 defects observed in ci^W/+ heterozygotes are enhanced by one copy of cg⁰⁷⁶⁵⁹. The abnormal leg disc morphology of cg¹/cg⁰⁷⁶⁵⁹ animals is substantially rescued by ci^{Scer\UAS.T:Ivir\HA1} expressed under the control of Scer\GAL4^ptc-559.1. The adult transverse row and sex comb phenotypes are also partially rescued.

(Svendsen et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

cg^Y4

(Campbell and Tomlinson, 2000)

cg³

(Svendsen et al., 2000)

Rescued by

cg⁰⁷⁶⁵⁹ is rescued by cg^+t11.5

(Campbell and Tomlinson, 2000)

Not rescued by

cg⁰⁷⁶⁵⁹ is not rescued by cg^tCG248

(Campbell and Tomlinson, 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12340

P{PZ}cg⁰⁷⁶⁵⁹ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Excision of the P{PZ} element can result in wild-type revertants.

(Campbell and Tomlinson, 2000)

Complements: l(2)00248⁰⁰²⁴⁸. Complements: l(2)00572⁰⁰⁵⁷². Complements: mam⁰³⁵⁰⁵. Complements: l(2)k05416^k05416. Complements: l(2)s3475^s3475.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

cg⁰⁷⁶⁵⁸

(Campbell and Tomlinson, 2000)

cg⁰⁷⁶⁵⁹

(McClure and Schubiger, 2008)

cg²

(Hitrik et al., 2016, Svendsen et al., 2000)

l(2)07569

(Svendsen et al., 2000)

l(2)07659

(Campbell and Tomlinson, 2000, Svendsen et al., 2000)

l(2)07659⁰⁷⁶⁵⁹

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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