FlyBase Allele Report: Dmel\Sin3A[08269]

General Information

Symbol

Dmel\Sin3A⁰⁸²⁶⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0008136

Feature type

allele

Associated gene

Dmel\Sin3A

Associated Insertion(s)

P{PZ}Sin3A⁰⁸²⁶⁹

Carried in Construct

Also Known As

l(2)08269, Sin3A^lof

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Sin3A⁰⁸²⁶⁹

Cytology

Description

Stated to be a P{lacW} insertion within an intron.

(Neufeld et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Cao et al., 2008)

exacerbates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

exacerbates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.GMR

(Read et al., 2005)

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Kontopoulos et al., 2006)

ameliorates neurodegenerative disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Steffan et al., 2001)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Steffan et al., 2001)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | chemical conditional

(St Laurent et al., 2013)

chemical resistant

(St Laurent et al., 2013)

lethal | recessive

(Spain et al., 2010, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die during embryonic stage

(Dobi et al., 2014)

long lived | chemical conditional

(St Laurent et al., 2013)

Phenotype Manifest In

embryonic/larval crystal cell

(Milchanowski et al., 2004)

larval somatic muscle cell

(Dobi et al., 2014)

larval somatic muscle cell (with Df(2R)Exel7121)

(Dobi et al., 2014)

lateral transverse muscle cell

(Dobi et al., 2014)

ventral acute muscle cell

(Dobi et al., 2014)

Detailed Description

Statement

Reference

Homozygous Sin3A⁰⁸²⁶⁹ embryos display a range of muscle phenotypes. Defects are observed in 100% of embryos and 100% of hemisegments. Early defects are not observed and a recognizable muscle pattern is generated. All die as embryos. Mature tendon cells are properly specified and positioned and embryos display a wild type cuticle pattern, indicating that ectodermal patterning is normal. Lateral transverse muscles (LTs 1-4) are often misshapen and frequently attach incorrectly; missing or unattached LTs are also frequently observed. Ventral acute muscles (VA1 and VA2) show less frequent defects, but muscle mis-attachment and mild changes in muscle shape are detected. Expression of 'identify genes' shows that the LT and VA muscles are specified correctly in Sin3A⁰⁸²⁶⁹ mutants; myoblast fusion also occurs.

(Dobi et al., 2014)

Sin3A⁰⁸²⁶⁹ flies reared on 62.5, 125, and 250υM rotenone-supplemented food display a robust resistance to rotenone-induced early mortality after 3 and 7 days and show a significant improvement in the odds of survival as compared to wild-type flies at 62.5 and 125υM rotenone doses after 3 days and at 125υM rotenone dose after 7 days.

Sin3A⁰⁸²⁶⁹ flies reared on 62.5, 125, and 250υM rotenone-supplemented food for 3 days exhibit a robust improvement in climbing ability and show a significant improvement in the odds of reaching the top section of the apparatus as compared to wild-type flies reared on 125υM rotenone-supplemented food.

Sin3A⁰⁸²⁶⁹ flies reared on 10mM sodium butyrate and 10mM sodium butyrate and 62.5, 125, and 250υM10mM sodium butyrate and 62.5, 125, and 250υM rotenone-supplemented food show a significant improvement in the odds of survival as compared to flies reared on rotenone-supplemented food without sodium butyrate. There is no additive effect of sodium butyrate-supplementation in Sin3A⁰⁸²⁶⁹ flies on rotenone-induced locomotor impairment.

Sin3A⁰⁸²⁶⁹ flies reared on 125&ugrM rotenone-supplemented food show a significant dopamine deficiency as compared to vehicle-treated flies. However, Sin3A⁰⁸²⁶⁹ flies reared on 125υM rotenone-supplemented food display a non-significant trend for moderately higher dopamine content as compared to wild-type flies reared on 125υM rotenone-supplemented food.

(St Laurent et al., 2013)

One copy of Sin3A⁰⁸²⁶⁹ has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

The average number of crystal cells per embryo is reduced (to 14.1) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).

(Milchanowski et al., 2004)

Expression of Sin3A⁰⁸²⁶⁹ under the control of Scer\GAL4^GMR.PF does not result in a rough eye phenotype.

(Shulman and Feany, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of visible | dominant phenotype of H^P141

(Moshkin et al., 2009)

Sin3A⁰⁸²⁶⁹ is an enhancer of visible phenotype of Iswi^{K159R.UAS.Tag:HA}, Scer\GAL4^ey.PH

(Burgio et al., 2008)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of visible phenotype of Ret^GMR.PR

(Read et al., 2005)

NOT Enhancer of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a non-enhancer of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

Sin3A⁰⁸²⁶⁹ is a non-enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor of visible | dominant phenotype of N^55e11

(Moshkin et al., 2009)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor of visible phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Moshkin et al., 2009)

Sin3A⁰⁸²⁶⁹ is a suppressor of abnormal neuroanatomy | adult stage | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Kontopoulos et al., 2006)

Sin3A⁰⁸²⁶⁹ is a suppressor of abnormal neuroanatomy | adult stage | conditional phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^elav.PU

(Kontopoulos et al., 2006)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor of partially lethal phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Steffan et al., 2001)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor of abnormal neuroanatomy | progressive phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Steffan et al., 2001)

NOT Suppressor of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a non-suppressor of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

Sin3A⁰⁸²⁶⁹ is a non-suppressor of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Other

Statement

Reference

EcR^C300Y, Sin3A⁰⁸²⁶⁹ has viable phenotype

(Bodai, 2000.12.22)

EcR^C300Y, Sin3A⁰⁸²⁶⁹ has wild-type phenotype

(Bodai, 2000.12.22)

EcR^C300Y, Sin3A[+]/Sin3A⁰⁸²⁶⁹ has viable phenotype

(Bodai, 2000.12.22)

EcR^C300Y, Sin3A[+]/Sin3A⁰⁸²⁶⁹ has wild-type phenotype

(Bodai, 2000.12.22)

EcR^C300Y/EcR[+], Sin3A⁰⁸²⁶⁹ has viable phenotype

(Bodai, 2000.12.22)

EcR^C300Y/EcR[+], Sin3A⁰⁸²⁶⁹ has wild-type phenotype

(Bodai, 2000.12.22)

Phenotype Manifest In

Enhancer of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of wing vein L5 phenotype of H^P141

(Moshkin et al., 2009)

Sin3A⁰⁸²⁶⁹ is an enhancer of eye phenotype of Iswi^{K159R.UAS.Tag:HA}, Scer\GAL4^ey.PH

(Burgio et al., 2008)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of eye phenotype of Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Cao et al., 2008)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is an enhancer of eye phenotype of Ret^GMR.PR

(Read et al., 2005)

Sin3A⁰⁸²⁶⁹ is an enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Sin3A⁰⁸²⁶⁹ is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

NOT Enhancer of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a non-enhancer of eye phenotype of Egfr^E1

(Read et al., 2005)

Sin3A⁰⁸²⁶⁹ is a non-enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor of eye phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Moshkin et al., 2009)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor of wing vein phenotype of N^55e11

(Moshkin et al., 2009)

Sin3A⁰⁸²⁶⁹ is a suppressor of dopaminergic neuron | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Kontopoulos et al., 2006)

Sin3A⁰⁸²⁶⁹ is a suppressor of dopaminergic neuron | conditional phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^elav.PU

(Kontopoulos et al., 2006)

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a suppressor of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Steffan et al., 2001)

NOT Suppressor of

Statement

Reference

Sin3A[+]/Sin3A⁰⁸²⁶⁹ is a non-suppressor of eye phenotype of Egfr^E1

(Read et al., 2005)

Sin3A⁰⁸²⁶⁹ is a non-suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Sin3A⁰⁸²⁶⁹ is a non-suppressor of eye phenotype of Scer\GAL4^hs.2sev, ttk^{69.UAS.Tag:HA}

(Wen et al., 2000)

Other

Statement

Reference

Scer\GAL4^Mef2.PR, Sin3A[+]/Sin3A⁰⁸²⁶⁹, ara^UAS.cGa has lateral transverse muscle cell phenotype

(Dobi et al., 2014)

Kr^UAS.cHa, Scer\GAL4^Mef2.PR, Sin3A[+]/Sin3A⁰⁸²⁶⁹ has ventral acute muscle cell phenotype

(Dobi et al., 2014)

Scer\GAL4^Mef2.PR, Sin3A[+]/Sin3A⁰⁸²⁶⁹, ara^UAS.cGa has larval somatic muscle cell phenotype

(Dobi et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^Mef2.PR-mediated expression of Kr^Scer\UAS.cHa in a Sin3A⁰⁸²⁶⁹/+ background results in 55% of embryos reproducibly displaying a muscle transformation of VA1 to VA2.

Scer\GAL4^Mef2.PR-mediated expression of ara^Scer\UAS.cGa in a Sin3A⁰⁸²⁶⁹/+ background results in embryos displaying aberrant muscle phenotypes, including an increased number of LT muscles.

Embryos trans-heterozygous for Sin3A⁰⁸²⁶⁹ and either Kr¹ Kr^mCD (a Kr loss-of-function allele) or Df(3L)iro-DFM3 (a deletion of ara and caup) do not show mesodermal defects.

(Dobi et al., 2014)

The small, rough eye phenotype caused by expression of asf1^Scer\UAS.cMa under the control of Scer\GAL4^ey.PH is significantly suppressed by Sin3A⁰⁸²⁶⁹/+.

The thickening of the wing veins that is seen in N^55e11/+ flies is suppressed by Sin3A⁰⁸²⁶⁹/+.

The truncation of wing vein L5 that is seen in H^P141/+ heterozygotes is enhanced by Sin3A⁰⁸²⁶⁹/+.

(Moshkin et al., 2009)

The severity of the mutant eye phenotype caused by expression of Iswi^{K159R.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH is enhanced by Sin3A⁰⁸²⁶⁹.

(Burgio et al., 2008)

EcR^C300Y/Sin3A⁰⁸²⁶⁹ transheterozygotes show normal viability and no visible phenotype.

(Bodai, 2000.12.22)

The eye phenotype caused by expression of ttk^{69.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev is not dramatically modulated by Sin3A⁰⁸²⁶⁹.

(Wen et al., 2000)

Xenogenetic Interactions

Statement

Reference

One copy of Sin3A⁰⁸²⁶⁹ moderately suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Sin3A⁰⁸²⁶⁹ rescues the progressive loss of dorsomedial dopamine neurons in the brain seen in flies expressing either Hsap\SNCA^Scer\UAS.cFa or Hsap\SNCA^{A30P.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Kontopoulos et al., 2006)

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not modified if the flies are also carrying Sin3A⁰⁸²⁶⁹.

(Shulman and Feany, 2003)

Sin3A⁰⁸²⁶⁹/+ increases the viability of flies expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav-C155 from 29% to 69% and reduces the extent and rate of neurodegeneration seen in these flies.

(Steffan et al., 2001)

Complementation and Rescue Data

Complements

Sin3A²⁶

(Razzaq et al., 2001)

Fails to complement

Sin3A^unspecified

(Neufeld et al., 1998)

Sin3A^k07423a

(BDGP Project Members, 1994-1999)

Sin3A^k08919

(BDGP Project Members, 1994-1999)

Sin3A^k09715

(BDGP Project Members, 1994-1999)

Sin3A^k02703

(BDGP Project Members, 1994-1999)

Sin3A^k07401

(BDGP Project Members, 1994-1999)

Sin3A^k05415

(BDGP Project Members, 1994-1999)

Partially rescued by

Sin3A⁰⁸²⁶⁹ is partially rescued by Sin3A^{220.UAS.Tag:HA}/Scer\GAL4^αTub84B.PL

(Spain et al., 2010)

Sin3A⁰⁸²⁶⁹ is partially rescued by Sin3A^{220.UAS.Tag:HA}/Sin3A^{187.UAS.Tag:HA}/Scer\GAL4^αTub84B.PL

(Spain et al., 2010)

Not rescued by

Sin3A⁰⁸²⁶⁹ is not rescued by Sin3A^{187.UAS.Tag:HA}/Scer\GAL4^αTub84B.PL

(Spain et al., 2010)

Comments

The lethality of Sin3A⁰⁸²⁶⁹ homozygotes is not rescued by expression of Sin3A^{187.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PL.

The lethality of Sin3A⁰⁸²⁶⁹ homozygotes is partially rescued by expression of Sin3A^{220.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PL (74% viability).

The lethality of Sin3A⁰⁸²⁶⁹ homozygotes is partially rescued by co-expression of both Sin3A^{187.Scer\UAS.T:Ivir\HA1} and Sin3A^{220.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PL (88% viability).

(Spain et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12350

P{PZ}Sin3A⁰⁸²⁶⁹ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: unch^k15501.

(Prokopenko et al., 2000)

Complements: l(2)k03003^k03003. Complements: spt4^k05316. Complements: l(2)k17019a^k17019a.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

P12350

(Milchanowski et al., 2004)

P2350

(Leventis et al., 2001)

Sin3A⁰⁸²⁶⁸

(Spain et al., 2010)

Sin3A⁰⁸²⁶⁹

(Reim, 2024.4.19, Sekiya et al., 2017, Sarov et al., 2016, Dobi et al., 2014, Lee and Spradling, 2014, Das et al., 2013, Debruhl et al., 2013, Sinenko et al., 2010, Moshkin et al., 2009, Burgio et al., 2008, Cao et al., 2008, Kontopoulos et al., 2006, Steffan et al., 2001)

Sin3A^LOF

(Sekiya et al., 2017)

Sin3A^k08269

(Razzaq et al., 2001)

Sin3A^lof

(St Laurent et al., 2013)

l(2)08269

(Shulman and Feany, 2003, Spradling et al., 1999)

l(2)08269⁰⁸²⁶⁹

l(2)8269

(Neufeld et al., 1998)

l(2)k08268

(Leventis et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (29)

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