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General Information
Symbol
Dmel\spin10403
Species
D. melanogaster
Name
FlyBase ID
FBal0008147
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)10403, spin4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
P{PZ} insertion within the spin transcription unit.
Insertion in the middle of exon 1.
Insertion components
P{PZ}spin10403
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Unlike controls, spin10403/spink09905 and spin10403/spinDelta58 transheterozygotes accumulate lamellar/multi-lamellar whorls and lysosomes in the adult brain. spin10403/spinDelta58 adults exhibit a progressive impairment in climbing ability, as compared to spinDelta58 heterozygous controls.
Upon starvation, fatbody cells of spin10403/spink09905 mutants accumulate abnormally enlarged P{tub-GFP-LAMP1}-positive structures.
Homozygous third larval instar eye imaginal discs show a number of defects in morphology and glial cell migration. In 9% of discs there is a duplication of the morphogenetic furrow and the corresponding field of photoreceptor neurons at 90[o] to the normal eye field. In 62% of discs, glial cells are found anterior to the morphogenetic furrow and appear to migrate in a broad stream onto the eye imaginal disc. In 11% of discs, only a relatively thin stream of of glial cells overshoot anterior to the morphogenetic furrow. In another 11% of discs, glial cells are found anterior to the position they are found in wild type (including sometimes crossing the morphogenetic furrow), but do not form a stream of cells anterior to the morphogenetic furrow. 7% of the discs appear wild type. Almost all of the ectopically positioned glial cells are perineurial glia. The homozygous discs generally contain more glial cells than normal, which is particularly apparent in young eye imaginal discs with 0-5 rows of photoreceptors cells. The defects in glial cell migration are seen in early third larval instar eye discs, with glial cells migrating far onto the undifferentiated epithelium (in wild type, glial cell migration from the optic stalk to the eye disc is inhibited at the beginning of the third instar stage). The shape of the carpet glial cells is severely affected in the mutants and they fail to extend over the eye field. Wrapping glia cells are found at a lower frequency in homozygous glial clones in the eye disc compared with wild-type clones, whereas the frequency of perineurial glial cells appears higher in the mutant clones. Homozygous carpet glia cells are reduced in size compared to wild-type cells.
65% of spinEP822/spin10403 stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 52% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.
Mutation is lethal at the pharate pupal stage. spin10403/spink09905 animals show a significant increase in bouton number at the larval neuromuscular junction (assayed at muscles 6/7 in hemisegment A3) compared to wild type. Quantal content at the neuromuscular junction is decreased by approximately 50% compared to wild type in spin10403/spink09905 animals. Quantal size is unaffected. Only 23% of spin10403/spink09905 animals are adult viable.
spin1/spin10403 females show a profound decrease in mating success compared to wild-type females when paired with wild-type males. The time spent by the wild-type male performing unilateral wing vibration during a 10 minute observation (the SAPI - sex appeal parameter index) is almost identical for males paired with spin1/spin10403 or wild-type females. spin1/spin10403 females show a number of rejection behaviours when paired with wild-type males, including fending, kicking, flicking, curling, punching and decamping, although extrusion is rarely seen. In addition, the mutant females show spreading behaviour (they raise their abdomens while spreading their vaginal plates), which is not seen in wild-type females. The mutant females show a high frequency of punching behaviour, which is rare amongst wild-type females. Homozygous animals show autofluorescence in the central nervous system from the larval stage onwards.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
spin10403 has neuroanatomy defective phenotype, suppressible by Hrs[+]/HrsD28
spin10403 has neuroanatomy defective phenotype, suppressible by wg[+]/wgl-8
spin10403 has neuroanatomy defective phenotype, suppressible by Mad8-2/Mad[+]
spin10403 has neuroanatomy defective phenotype, suppressible by dppd6/dpp[+]
spin10403 has neuroanatomy defective phenotype, suppressible by dppd12/dpp[+]
spin10403 has neuroanatomy defective phenotype, suppressible by tkv7/tkv[+]
NOT suppressed by
Statement
Reference
spin10403 has neuroanatomy defective phenotype, non-suppressible by dome[+]/dome60441
spin10403 has neuroanatomy defective phenotype, non-suppressible by os[+]/upd1YM55
spin10403 has neuroanatomy defective phenotype, non-suppressible by put[+]/put10460
spin10403 has neuroanatomy defective phenotype, non-suppressible by witB11/wit[+]
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
spin10403 has eye disc phenotype, suppressible by Hrs[+]/HrsD28
spin10403 has glial cell phenotype, suppressible by Hrs[+]/HrsD28
spin10403 has eye disc phenotype, suppressible by wg[+]/wgl-8
spin10403 has glial cell phenotype, suppressible by wg[+]/wgl-8
spin10403 has eye disc phenotype, suppressible by Mad8-2/Mad[+]
spin10403 has glial cell phenotype, suppressible by Mad8-2/Mad[+]
spin10403 has eye disc phenotype, suppressible by dppd6/dpp[+]
spin10403 has glial cell phenotype, suppressible by dppd6/dpp[+]
spin10403 has eye disc phenotype, suppressible by dppd12/dpp[+]
spin10403 has glial cell phenotype, suppressible by dppd12/dpp[+]
spin10403 has eye disc phenotype, suppressible by sax[+]/saxKG07525
spin10403 has glial cell phenotype, suppressible by sax[+]/saxKG07525
spin10403 has eye disc phenotype, suppressible by tkv7/tkv[+]
spin10403 has glial cell phenotype, suppressible by tkv7/tkv[+]
spin10403 has eye disc phenotype, suppressible by tkv1/tkv7
spin10403 has glial cell phenotype, suppressible by tkv1/tkv7
spink09905/spin10403 has bouton | supernumerary phenotype, suppressible by witA12/wit[+]
spink09905/spin10403 has bouton | supernumerary phenotype, suppressible by tkv7/tkv[+]
spink09905/spin10403 has bouton | supernumerary phenotype, suppressible by sax4/sax[+]
NOT suppressed by
Statement
Reference
spin10403 has eye disc phenotype, non-suppressible by dome[+]/dome60441
spin10403 has glial cell phenotype, non-suppressible by dome[+]/dome60441
spin10403 has eye disc phenotype, non-suppressible by os[+]/upd1YM55
spin10403 has glial cell phenotype, non-suppressible by os[+]/upd1YM55
spin10403 has eye disc phenotype, non-suppressible by put[+]/put10460
spin10403 has glial cell phenotype, non-suppressible by put[+]/put10460
spin10403 has eye disc phenotype, non-suppressible by witB11/wit[+]
spin10403 has glial cell phenotype, non-suppressible by witB11/wit[+]
Additional Comments
Genetic Interactions
Statement
Reference
HrsD28/+ suppresses the glial migration defects seen in spin10403 eye discs. The glial overmigration phenotype seen in spin10403 eye discs is significantly suppressed if the animals are also heterozygous for one of wgl-8, Mad8-2, dppd6, dppd12, saxKG07525 or tkv7. The glial overmigration phenotype seen in spin10403 eye discs is significantly suppressed if the animals are also carrying tkv7/saxKG07525 or tkv1/tkv7. The glial overmigration phenotype seen in spin10403 eye discs is significantly suppressed if the animals are also expressing tkvVDRC.cUa under the control of Scer\GAL4repo.PU. The glial overmigration phenotype seen in spin10403 eye discs is not suppressed if the animals are also heterozygous for dome60441, upd1YM55, put10460 or witB11.
One copy of witA12, tkv7 or sax4 suppresses the increased bouton number seen at the neuromuscular junction in spin10403/spink09905 animals. spin10403/spink09905 animals which are also mutant for witA12/witB11, tkv7/tkvk16713 or sax4/Df(2R)cn7969 show a further decrease in bouton number, either to wild type or below wild type levels.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
spinI.Scer\UAS in the absence of a Scer\GAL4 driver can weakly rescue the eye disc defects of spin10403 larvae. Expression of spinI.Scer\UAS under the control of Scer\GAL4da.G32 strongly rescues the glial migration and morphology defects seen in spin10403 eye discs. Expression of spinI.Scer\UAS under the control of either Scer\GAL4spin.PN or Scer\GAL4repo.PU partially rescues the glial migration and morphology defects seen in spin10403 eye discs. Expression of spinI.Scer\UAS under the control of either Scer\GAL4elav.PU, Scer\GAL4ey.3.5.Exel, Scer\GAL4c527 or Scer\GAL4Mz97 only slightly ameliorates the glial migration and morphology defects seen in spin10403 eye discs. Expression of spinI.Scer\UAS under the control of Scer\GAL4moody.SPG does not significantly rescue the eye disc defects of spin10403 larvae.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
A. Spradling.
Comments
Comments
Complements: l(2)0507005070. Complements: bnchk02511. Complements: spink06110. Complements: spink07302. Complements: spink09905. Complements: l(2)k15617k15617.
Precise excision of the insertion reverts all mutant eye disc phenotypes.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (13)