FlyBase Allele Report: Dmel\spin[10403]

Unlike controls, spin¹⁰⁴⁰³/spin^k09905 and spin¹⁰⁴⁰³/spin^Delta58 transheterozygotes accumulate lamellar/multi-lamellar whorls and lysosomes in the adult brain.

spin¹⁰⁴⁰³/spin^Delta58 adults exhibit a progressive impairment in climbing ability, as compared to spin^Delta58 heterozygous controls.

(Hebbar et al., 2017)

Upon starvation, fatbody cells of spin¹⁰⁴⁰³/spin^k09905 mutants accumulate abnormally enlarged P{tub-GFP-LAMP1}-positive structures.

(Rong et al., 2011)

Homozygous third larval instar eye imaginal discs show a number of defects in morphology and glial cell migration. In 9% of discs there is a duplication of the morphogenetic furrow and the corresponding field of photoreceptor neurons at 90[o] to the normal eye field. In 62% of discs, glial cells are found anterior to the morphogenetic furrow and appear to migrate in a broad stream onto the eye imaginal disc. In 11% of discs, only a relatively thin stream of of glial cells overshoot anterior to the morphogenetic furrow. In another 11% of discs, glial cells are found anterior to the position they are found in wild type (including sometimes crossing the morphogenetic furrow), but do not form a stream of cells anterior to the morphogenetic furrow. 7% of the discs appear wild type. Almost all of the ectopically positioned glial cells are perineurial glia. The homozygous discs generally contain more glial cells than normal, which is particularly apparent in young eye imaginal discs with 0-5 rows of photoreceptors cells. The defects in glial cell migration are seen in early third larval instar eye discs, with glial cells migrating far onto the undifferentiated epithelium (in wild type, glial cell migration from the optic stalk to the eye disc is inhibited at the beginning of the third instar stage). The shape of the carpet glial cells is severely affected in the mutants and they fail to extend over the eye field.

Wrapping glia cells are found at a lower frequency in homozygous glial clones in the eye disc compared with wild-type clones, whereas the frequency of perineurial glial cells appears higher in the mutant clones. Homozygous carpet glia cells are reduced in size compared to wild-type cells.

(Yuva-Aydemir et al., 2011)

65% of spin^EP822/spin¹⁰⁴⁰³ stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 52% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

(Bass et al., 2009)

Mutation is lethal at the pharate pupal stage. spin¹⁰⁴⁰³/spin^k09905 animals show a significant increase in bouton number at the larval neuromuscular junction (assayed at muscles 6/7 in hemisegment A3) compared to wild type. Quantal content at the neuromuscular junction is decreased by approximately 50% compared to wild type in spin¹⁰⁴⁰³/spin^k09905 animals. Quantal size is unaffected. Only 23% of spin¹⁰⁴⁰³/spin^k09905 animals are adult viable.

(Sweeney and Davis, 2002)

spin¹/spin¹⁰⁴⁰³ females show a profound decrease in mating success compared to wild-type females when paired with wild-type males. The time spent by the wild-type male performing unilateral wing vibration during a 10 minute observation (the SAPI - sex appeal parameter index) is almost identical for males paired with spin¹/spin¹⁰⁴⁰³ or wild-type females. spin¹/spin¹⁰⁴⁰³ females show a number of rejection behaviours when paired with wild-type males, including fending, kicking, flicking, curling, punching and decamping, although extrusion is rarely seen. In addition, the mutant females show spreading behaviour (they raise their abdomens while spreading their vaginal plates), which is not seen in wild-type females. The mutant females show a high frequency of punching behaviour, which is rare amongst wild-type females. Homozygous animals show autofluorescence in the central nervous system from the larval stage onwards.

(Nakano et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

spin^k09905/spin¹⁰⁴⁰³ has increased mortality during development phenotype, suppressible | partially by CDase[+]/CDase^slab-2

(Hebbar et al., 2017)

spin^k09905/spin¹⁰⁴⁰³ has increased mortality during development phenotype, suppressible | partially by CDase^UAS.cRa/Scer\GAL4^spin.PN

(Hebbar et al., 2017)

spin^Delta58/spin¹⁰⁴⁰³ has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible | partially by CDase^UAS.cRa/Scer\GAL4^spin.PN

(Hebbar et al., 2017)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by Hrs[+]/Hrs^D28

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by wg[+]/wg^l-8

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by Mad^8-2/Mad[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by dpp^d6/dpp[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by dpp^d12/dpp[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by sax[+]/sax^KG07525

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by tkv⁷/tkv[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by tkv⁷/sax^KG07525

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by tkv¹/tkv⁷

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^repo.PU/tkv^VDRC.cUa

(Yuva-Aydemir et al., 2011)

spin^k09905/spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by wit^A12/wit[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by sax⁴/sax[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by sax⁴/Df(2R)cn7969

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by tkv⁷/tkv[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by tkv^k16713/tkv⁷

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, suppressible by wit^B11/wit^A12

(Sweeney and Davis, 2002)

NOT suppressed by

Statement

Reference

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, non-suppressible by put[+]/put¹⁰⁴⁶⁰

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, non-suppressible by wit^B11/wit[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, non-suppressible by dome[+]/dome⁶⁰⁴⁴¹

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has abnormal neuroanatomy phenotype, non-suppressible by os[+]/upd1^YM55

(Yuva-Aydemir et al., 2011)

Other

Statement

Reference

CDase[+]/CDase^slab-2, spin^k09905/spin¹⁰⁴⁰³ has increased mortality during development phenotype

(Hebbar et al., 2017)

CDase^UAS.cRa, Scer\GAL4^spin.PN, spin^k09905/spin¹⁰⁴⁰³ has increased mortality during development phenotype

(Hebbar et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

spin^Delta58/spin¹⁰⁴⁰³ has adult brain | progressive phenotype, enhanceable by CDase[+]/CDase^slab-2

(Hebbar et al., 2017)

Suppressed by

Statement

Reference

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by Hrs[+]/Hrs^D28

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by Hrs[+]/Hrs^D28

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by wg[+]/wg^l-8

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by wg[+]/wg^l-8

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by Mad^8-2/Mad[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by Mad^8-2/Mad[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by dpp^d6/dpp[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by dpp^d6/dpp[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by dpp^d12/dpp[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by dpp^d12/dpp[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by sax[+]/sax^KG07525

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by sax[+]/sax^KG07525

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by tkv⁷/tkv[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by tkv⁷/tkv[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by tkv⁷/sax^KG07525

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by tkv⁷/sax^KG07525

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by tkv¹/tkv⁷

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by tkv¹/tkv⁷

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, suppressible by Scer\GAL4^repo.PU/tkv^VDRC.cUa

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, suppressible by Scer\GAL4^repo.PU/tkv^VDRC.cUa

(Yuva-Aydemir et al., 2011)

spin^k09905/spin¹⁰⁴⁰³ has bouton | increased number phenotype, suppressible by sax⁴/sax[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has bouton | increased number phenotype, suppressible by sax⁴/Df(2R)cn7969

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has bouton | increased number phenotype, suppressible by tkv⁷/tkv[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has bouton | increased number phenotype, suppressible by tkv^k16713/tkv⁷

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has bouton | increased number phenotype, suppressible by wit^A12/wit[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has bouton | increased number phenotype, suppressible by wit^B11/wit^A12

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has neuromuscular junction phenotype, suppressible by sax⁴/sax[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has neuromuscular junction phenotype, suppressible by sax⁴/Df(2R)cn7969

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has neuromuscular junction phenotype, suppressible by tkv⁷/tkv[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has neuromuscular junction phenotype, suppressible by tkv^k16713/tkv⁷

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has neuromuscular junction phenotype, suppressible by wit^A12/wit[+]

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ has neuromuscular junction phenotype, suppressible by wit^B11/wit^A12

(Sweeney and Davis, 2002)

NOT suppressed by

Statement

Reference

spin¹⁰⁴⁰³ has eye disc phenotype, non-suppressible by dome[+]/dome⁶⁰⁴⁴¹

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, non-suppressible by dome[+]/dome⁶⁰⁴⁴¹

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, non-suppressible by os[+]/upd1^YM55

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, non-suppressible by os[+]/upd1^YM55

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, non-suppressible by put[+]/put¹⁰⁴⁶⁰

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, non-suppressible by put[+]/put¹⁰⁴⁶⁰

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has eye disc phenotype, non-suppressible by wit^B11/wit[+]

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ has glial cell phenotype, non-suppressible by wit^B11/wit[+]

(Yuva-Aydemir et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Hrs^D28/+ suppresses the glial migration defects seen in spin¹⁰⁴⁰³ eye discs.

The glial overmigration phenotype seen in spin¹⁰⁴⁰³ eye discs is significantly suppressed if the animals are also heterozygous for one of wg^l-8, Mad^8-2, dpp^d6, dpp^d12, sax^KG07525 or tkv⁷.

The glial overmigration phenotype seen in spin¹⁰⁴⁰³ eye discs is significantly suppressed if the animals are also carrying tkv⁷/sax^KG07525 or tkv¹/tkv⁷.

The glial overmigration phenotype seen in spin¹⁰⁴⁰³ eye discs is significantly suppressed if the animals are also expressing tkv^VDRC.cUa under the control of Scer\GAL4^repo.PU.

The glial overmigration phenotype seen in spin¹⁰⁴⁰³ eye discs is not suppressed if the animals are also heterozygous for dome⁶⁰⁴⁴¹, upd1^YM55, put¹⁰⁴⁶⁰ or wit^B11.

(Yuva-Aydemir et al., 2011)

One copy of wit^A12, tkv⁷ or sax⁴ suppresses the increased bouton number seen at the neuromuscular junction in spin¹⁰⁴⁰³/spin^k09905 animals. spin¹⁰⁴⁰³/spin^k09905 animals which are also mutant for wit^A12/wit^B11, tkv⁷/tkv^k16713 or sax⁴/Df(2R)cn7969 show a further decrease in bouton number, either to wild type or below wild type levels.

(Sweeney and Davis, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

spin^Δ2b

(Sweeney and Davis, 2002)

spin^08639a

(BDGP Project Members, 1994-1999)

Rescued by

spin^k09905/spin¹⁰⁴⁰³ is rescued by Scer\GAL4^αTub84B.PL/spin^UAS.cSa

(Sweeney and Davis, 2002)

spin¹⁰⁴⁰³ is rescued by spin^I.UAS/Scer\GAL4^spin.PN

(Nakano et al., 2001)

Partially rescued by

spin^k09905/spin¹⁰⁴⁰³ is partially rescued by spin^{UAS.EGFP,Tag:MYC}/Scer\GAL4^spin.PN

(Hebbar et al., 2017)

spin¹⁰⁴⁰³ is partially rescued by spin^I.UAS/Scer\GAL4^spin.PN

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ is partially rescued by spin^I.UAS/Scer\GAL4^da.G32

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ is partially rescued by spin^I.UAS/Scer\GAL4^repo.PU

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ is partially rescued by spin^I.UAS/Scer\GAL4^elav.PU

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ is partially rescued by spin^I.UAS/Scer\GAL4^ey.3.5.Exel

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ is partially rescued by spin^I.UAS/Scer\GAL4^c527

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ is partially rescued by spin^I.UAS/Scer\GAL4^Ntan1-Mz97

(Yuva-Aydemir et al., 2011)

spin^k09905/spin¹⁰⁴⁰³ is partially rescued by Scer\GAL4^Mhc.PW/spin^UAS.cSa

(Sweeney and Davis, 2002)

spin^k09905/spin¹⁰⁴⁰³ is partially rescued by Scer\GAL4^elav.PLu/spin^UAS.cSa

(Sweeney and Davis, 2002)

Not rescued by

spin¹⁰⁴⁰³ is not rescued by spin^I.UAS/Scer\GAL4^moody.SPG

(Yuva-Aydemir et al., 2011)

spin¹⁰⁴⁰³ is not rescued by spin^III.hs

(Nakano et al., 2001)

spin¹⁰⁴⁰³ is not rescued by Scer\GAL4^spin.PN/spin^IV.UAS

(Nakano et al., 2001)

spin¹⁰⁴⁰³ is not rescued by Scer\GAL4^spin.PN/spin^II.UAS

(Nakano et al., 2001)

spin¹⁰⁴⁰³ is not rescued by Scer\GAL4^spin.PN/spin^V.UAS

(Nakano et al., 2001)

Comments

spin^I.Scer\UAS in the absence of a Scer\GAL4 driver can weakly rescue the eye disc defects of spin¹⁰⁴⁰³ larvae.

Expression of spin^I.Scer\UAS under the control of Scer\GAL4^da.G32 strongly rescues the glial migration and morphology defects seen in spin¹⁰⁴⁰³ eye discs.

Expression of spin^I.Scer\UAS under the control of either Scer\GAL4^spin.PN or Scer\GAL4^repo.PU partially rescues the glial migration and morphology defects seen in spin¹⁰⁴⁰³ eye discs.

Expression of spin^I.Scer\UAS under the control of either Scer\GAL4^elav.PU, Scer\GAL4^ey.3.5.Exel, Scer\GAL4^c527 or Scer\GAL4^Mz97 only slightly ameliorates the glial migration and morphology defects seen in spin¹⁰⁴⁰³ eye discs.

Expression of spin^I.Scer\UAS under the control of Scer\GAL4^moody.SPG does not significantly rescue the eye disc defects of spin¹⁰⁴⁰³ larvae.

(Yuva-Aydemir et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12365

P{PZ}spin¹⁰⁴⁰³ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(2)05070⁰⁵⁰⁷⁰. Complements: bnch^k02511. Complements: spin^k06110. Complements: spin^k07302. Complements: spin^k09905. Complements: l(2)k15617^k15617.

(BDGP Project Members, 1994-1999)

Precise excision of the insertion reverts all mutant eye disc phenotypes.

(Yuva-Aydemir et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Spin⁴

(Walsh et al., 2021)

l(2)10403

(Sweeney and Davis, 2002, Nakano et al., 2001, Spradling et al., 1999)

l(2)10403¹⁰⁴⁰³

(BDGP Project Members, 1994-1999)

spin4

(Sweeney and Davis, 2002)

spin¹⁰⁴⁰³

(Rong et al., 2011)

spin⁴

(Hebbar et al., 2017, Milton et al., 2011)

spin^P2

(Nakano et al., 2001)

spin^P

(Yuva-Aydemir et al., 2011)

spin^l(2)10403

(Yuva-Aydemir et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (14)

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