Allele Dmel\Sdc¹⁰⁶⁰⁸

General Information
Symbol	Dmel\Sdc¹⁰⁶⁰⁸	Species	D. melanogaster
Name		FlyBase ID	FBal0008157
Feature type	allele	Associated gene	Dmel\Sdc
Also Known As	l(2)10608

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms larval stage NMJ bouton
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:17,364,231..17,364,231 (Rawson, 2005, BDGP Project Members, 1994-1999, Rawson et al., 2005, Fox and Zinn, 2005, Fox and Zinn, 2005, Lincecum et al., 2000, Spradling et al., 1999, Ren et al., 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{PZ}Sdc¹⁰⁶⁰⁸ (Rawson, 2005, BDGP Project Members, 1994-1999, Rawson et al., 2005, Fox and Zinn, 2005, Fox and Zinn, 2005, Lincecum et al., 2000, Spradling et al., 1999, Ren et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999) neuroanatomy defective (Johnson et al., 2004) neuroanatomy defective (with Sdc⁴⁸) (Rawson et al., 2005) neuroanatomy defective \| larval stage (Johnson et al., 2006) neuroanatomy defective \| larval stage (with Sdc⁴⁸), with Sara^Ubi.PJ (Johnson et al., 2006) neurophysiology defective (with Sdc⁴⁸) (Rawson et al., 2005)
Phenotype Manifest In
	eye photoreceptor cell & axon (with Sdc⁴⁸) (Rawson et al., 2005) lamina plexus (with Sdc⁴⁸) (Rawson et al., 2005) medulla (with Sdc⁴⁸) (Rawson et al., 2005) NMJ bouton \| larval stage (Johnson et al., 2006) NMJ bouton \| larval stage (with Sdc⁴⁸), with Sara^Ubi.PJ (Johnson et al., 2006) presumptive embryonic/larval central nervous system (Johnson et al., 2004)
Detailed Description
	Statement Reference Homozygous larvae show a significant reduction in bouton number at the neuromuscular junction compared to wild type. Sdc[10608]/Df(2R)48 larvae (carrying Sara[Ubi.PJ] to provide Sara function) show a significant reduction in bouton number at the neuromuscular junction compared to wild type. Mean active zone area and number of active zones per bouton are normal. The amplitude of the evoked excitatory junctional potential (EJP) is normal at the neuromuscular junction of Sdc[10608]/Df(2R)48 larvae (carrying Sara[Ubi.PJ] to provide Sara function). The amplitude and frequency of miniature EJPs are not significantly different from normal. (Johnson et al., 2006) Sdc¹⁰⁶⁰⁸ mutants display ISNb bypass at a similar rate to wild-type embryos. (Fox and Zinn, 2005) Half of Df(2R)48/Sdc¹⁰⁶⁰⁸ larvae (in which Sara function has been rescued by Sara^Ubi.PJ) show photoreceptor projection and lamina-plexus defects. At the pupal level, these mutants show crossover of R7 axons between medullary cartridges and defective axon pathfinding to the medulla, with a low penetrance of R7/R8 terminal disruption. Electrophysiological analysis shows that as adults, these mutants have grossly abnormal ERGs, with defective photoreceptor polarization and complete absence of on- and off-transients. (Rawson et al., 2005) Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ) have no rough eye phenotype and show no defects in the specification of photoreceptors, glia or lamina neurons. (Rawson, 2005) Sdc¹⁰⁶⁰⁸ embryos show a high frequency of axons crossing the midline in the central nervous system. (Johnson et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has neuroanatomy defective phenotype, suppressible \| partially by Scer\GAL4^elav-C155/dlp^{Scer\UAS.T:Ivir\HA1} (Rawson et al., 2005)
NOT suppressed by
Statement Reference Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has neurophysiology defective phenotype, non-suppressible by Scer\GAL4^elav-C155/dlp^{Scer\UAS.T:Ivir\HA1} (Rawson et al., 2005) Sdc¹⁰⁶⁰⁸ has neuroanatomy defective \| larval stage phenotype, non-suppressible by dlp^Scer\UAS.cBa/Scer\GAL4^how-24B (Johnson et al., 2006)
Phenotype Manifest In
Suppressed by
Statement Reference Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has medulla phenotype, suppressible by Scer\GAL4^elav-C155/dlp^{Scer\UAS.T:Ivir\HA1} (Rawson et al., 2005)
NOT suppressed by
Statement Reference Sdc¹⁰⁶⁰⁸ has NMJ bouton \| larval stage phenotype, non-suppressible by dlp^Scer\UAS.cBa/Scer\GAL4^how-24B (Johnson et al., 2006)
Enhancer of
Statement Reference Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is an enhancer of anterior fascicle & axon \| ectopic phenotype of Lar^5.5/Lar^13.2 (Fox and Zinn, 2005) Sdc¹⁰⁶⁰⁸ is an enhancer of anterior fascicle & axon \| ectopic phenotype of Lar^bypass (Fox and Zinn, 2005)
Additional Comments
Genetic Interactions
Statement Reference Expression of dlp[Scer\UAS.cBa] under the control of Scer\GAL4[how-24B] does not rescue the reduction in bouton number at the neuromuscular junction seen in homozygous Sdc[10608] larvae. (Johnson et al., 2006) Sdc¹⁰⁶⁰⁸ increases the penetrance of the Lar^bypass ISNb bypass phenotype by more than 2-fold. Sdc⁴⁸/Sdc¹⁰⁶⁰⁸; Lar^13.2/Lar^5.5 (in which Sara has been rescued by expression of the Sara^Ubi.PJ transgene) mutants display an increased penetrance of the bypass phenotype (43% vs. 28%) relative to the corresponding Lar^13.2/Lar^5.5 transheterozygote. (Fox and Zinn, 2005) Expression of dlp^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^elav-C155, rescues the medulla patterning defects of Sdc¹⁰⁶⁰⁸/Df(2R)48 mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue the ERG defects. (Rawson et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Sdc^k10215 (BDGP Project Members, 1994-1999)
Rescued by	Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is rescued by Scer\GAL4^elav.PU/Sdc^Scer\UAS.cJa (Johnson et al., 2006)
Partially rescued by	Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is partially rescued by Scer\GAL4^elav-C155/Sdc^Scer\UAS.cSa (Rawson et al., 2005) Sdc¹⁰⁶⁰⁸ is partially rescued by Scer\GAL4^how-24B/Sdc^Scer\UAS.cJa (Johnson et al., 2006, Johnson et al., 2006)
Comments	Expression of Sdc[Scer\UAS.cJa] under the control of Scer\GAL4[elav.PU] almost completely rescues the reduction in bouton number at the neuromuscular junction which is seen in Sdc[10608]/Df(2R)48 larvae (carrying Sara[Ubi.PJ] to provide Sara function). Expression of Sdc[Scer\UAS.cJa] under the control of Scer\GAL4[how-24B] provides limited rescue of the reduction in bouton number at the neuromuscular junction which is seen in homozygous Sdc[10608] larvae. (Johnson et al., 2006) Expression of Sdc^Scer\UAS.cSa, under the control of Scer\GAL4^elav-C155, rescues the medulla cartridge crossover and R7/R8 termini disruption pupal phenotypes of Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue photoreceptor projection defects to the larval lamina or ERG abnormalities in adults. (Rawson et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer	A. Spradling.

Comments
	Complements: Fkbp13⁰⁰⁷³⁴. Complements: MESK2⁰¹⁴⁶⁷. Complements: dom^k08108. Complements: l(2)k10317^k10317. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	l(2)10608 (Lincecum et al., 2000, Spradling et al., 1999) l(2)10608¹⁰⁶⁰⁸ (BDGP Project Members, 1994-1999, ) sdc¹⁰⁶⁰⁸ (Johnson et al., 2004, Ren et al., 2009) Sdc¹⁰⁶⁰⁸ (De Luca et al., 2010) Sdc^l(2)10608 (Johnson et al., 2006) Sdc^P2377 (Johnson et al., 2006) Sdc^P (Johnson et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	De Luca et al., 2010, PLoS ONE 5(6): e11286 A conserved role for syndecan family members in the regulation of whole-body energy metabolism. [FBrf0211150] Ren et al., 2009, J. Neurosci. 29(26): 8539--8550 Cell type-specific requirements for heparan sulfate biosynthesis at the Drosophila neuromuscular junction: effects on synapse function, membrane trafficking, and mitochondrial localization. [FBrf0208233] Johnson et al., 2006, Neuron 49(4): 517--531 The HSPGs Syndecan and Dallylike bind the receptor phosphatase LAR and exert distinct effects on synaptic development. [FBrf0191320] Fox and Zinn, 2005, Curr. Biol. 15(19): 1701--1711 The heparan sulfate proteoglycan syndecan is an in vivo ligand for the Drosophila LAR receptor tyrosine phosphatase. [FBrf0190011] Rawson et al., 2005, Curr. Biol. 15(9): 833--838 The heparan sulfate proteoglycans daily-like and syndecan have distinct functions in axon guidance and visual-system assembly in Drosophila. [FBrf0187328] Johnson et al., 2004, Curr. Biol. 14(6): 499--504 Axonal heparan sulfate proteoglycans regulate the distribution and efficiency of the repellent slit during midline axon guidance. [FBrf0174485] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Supplementary material	Fox and Zinn, 2005, Curr. Biol. 15(19): Supplemental data. [FBrf0191745] Rawson, 2005, Curr. Biol. 15(9): Document S1. Experimental Procedures and Four Figures. [FBrf0191734]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Lincecum et al., 2000, Mol. Biol. Cell 11(Suppl.): 145a--146a Conservation of function in syndecans: Drosophila syndecan and murine syndecan-1 cytoplasmic domains are required for epithelial morphogenesis. [FBrf0138476]

Allele Dmel\Sdc10608

Allele Dmel\Sdc¹⁰⁶⁰⁸