FlyBase Allele Report: Dmel\Sdc[10608]

(Thuveson et al., 2019, Ren et al., 2009, Fox and Zinn, 2005, Fox and Zinn, 2005, Rawson, 2005, Rawson et al., 2005, Lincecum et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye photoreceptor cell & axon (with Sdc⁴⁸)

(Rawson et al., 2005)

lamina plexus (with Sdc⁴⁸)

(Rawson et al., 2005)

medulla (with Sdc⁴⁸)

(Rawson et al., 2005)

NMJ bouton | larval stage

(Johnson et al., 2006)

NMJ bouton | larval stage (with Sdc⁴⁸), with Sara^Ubi.PJ

(Johnson et al., 2006)

NMJ bouton | third instar larval stage (with Df(2R)48)

(Nguyen et al., 2016)

presumptive embryonic/larval central nervous system

(Johnson et al., 2004)

Detailed Description

Statement

Reference

Homozygous larvae show a significant reduction in bouton number at the neuromuscular junction compared to wild type.

Sdc¹⁰⁶⁰⁸/Df(2R)48 larvae (carrying Sara^Ubi.PJ to provide Sara function) show a significant reduction in bouton number at the neuromuscular junction compared to wild type. Mean active zone area and number of active zones per bouton are normal.

The amplitude of the evoked excitatory junctional potential (EJP) is normal at the neuromuscular junction of Sdc¹⁰⁶⁰⁸/Df(2R)48 larvae (carrying Sara^Ubi.PJ to provide Sara function). The amplitude and frequency of miniature EJPs are not significantly different from normal.

(Johnson et al., 2006)

Sdc¹⁰⁶⁰⁸ mutants display ISNb bypass at a similar rate to wild-type embryos.

(Fox and Zinn, 2005)

Half of Df(2R)48/Sdc¹⁰⁶⁰⁸ larvae (in which Sara function has been rescued by Sara^Ubi.PJ) show photoreceptor projection and lamina-plexus defects. At the pupal level, these mutants show crossover of R7 axons between medullary cartridges and defective axon pathfinding to the medulla, with a low penetrance of R7/R8 terminal disruption. Electrophysiological analysis shows that as adults, these mutants have grossly abnormal ERGs, with defective photoreceptor polarization and complete absence of on- and off-transients.

(Rawson et al., 2005)

Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ) have no rough eye phenotype and show no defects in the specification of photoreceptors, glia or lamina neurons.

(Rawson, 2005)

Sdc¹⁰⁶⁰⁸ embryos show a high frequency of axons crossing the midline in the central nervous system.

(Johnson et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sdc¹⁰⁶⁰⁸/Df(2R)48 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^how-24B/Sdc::Hsap\PDGFRB^{TM-Swap.UAS.Tag:MYC}

(Nguyen et al., 2016)

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^elav-C155/dlp^UAS.Tag:HA

(Rawson et al., 2005)

NOT suppressed by

Statement

Reference

Sdc¹⁰⁶⁰⁸ has abnormal neuroanatomy | larval stage phenotype, non-suppressible by dlp^UAS.cBa/Scer\GAL4^how-24B

(Johnson et al., 2006)

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^elav-C155/dlp^UAS.Tag:HA

(Rawson et al., 2005)

Enhancer of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Enhancer of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Suppressor of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Sdc¹⁰⁶⁰⁸/Sdc[+] is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

NOT Suppressor of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sdc¹⁰⁶⁰⁸/Df(2R)48 has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^how-24B/Sdc::Hsap\PDGFRB^{TM-Swap.UAS.Tag:MYC}

(Nguyen et al., 2016)

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has medulla phenotype, suppressible by Scer\GAL4^elav-C155/dlp^UAS.Tag:HA

(Rawson et al., 2005)

NOT suppressed by

Statement

Reference

Sdc¹⁰⁶⁰⁸ has NMJ bouton | larval stage phenotype, non-suppressible by dlp^UAS.cBa/Scer\GAL4^how-24B

(Johnson et al., 2006)

Enhancer of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is an enhancer of anterior fascicle & axon | ectopic phenotype of Lar^5.5/Lar^13.2

(Fox and Zinn, 2005)

Sdc¹⁰⁶⁰⁸ is an enhancer of anterior fascicle & axon | ectopic phenotype of Lar^bypass

(Fox and Zinn, 2005)

NOT Enhancer of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is a non-enhancer of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Suppressor of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Sdc¹⁰⁶⁰⁸/Sdc[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

NOT Suppressor of

Statement

Reference

Sdc¹⁰⁶⁰⁸/Sdc[+] is a non-suppressor of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Other

Statement

Reference

Dys^RNAi.NH2.UAS, Sdc¹⁰⁶⁰⁸/Sdc[+] has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Dys^RNAi.C.UAS, Sdc¹⁰⁶⁰⁸/Sdc[+] has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The commissural axon phenotype (failure to cross the midline) seen in embryos expressing Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^P52 in a Sema-1a^k13702 null background is not affected if the embryos are also heterozygous for Sdc¹⁰⁶⁰⁸.

Sema-1a^k13702/+ ; Sdc¹⁰⁶⁰⁸/+ double heterozygous embryo do not show significant ISNb or SNa motor axon guidance defects compared to controls.

(Cho et al., 2012)

One copy of Sdc¹⁰⁶⁰⁸ moderately suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU but produces extra wing vein material.

One copy of Sdc¹⁰⁶⁰⁸ weakly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of Sdc¹⁰⁶⁰⁸ enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

Expression of dlp^Scer\UAS.cBa under the control of Scer\GAL4^how-24B does not rescue the reduction in bouton number at the neuromuscular junction seen in homozygous Sdc¹⁰⁶⁰⁸ larvae.

(Johnson et al., 2006)

Sdc¹⁰⁶⁰⁸ increases the penetrance of the Lar^bypass ISNb bypass phenotype by more than 2-fold. Sdc⁴⁸/Sdc¹⁰⁶⁰⁸; Lar^13.2/Lar^5.5 (in which Sara has been rescued by expression of the Sara^Ubi.PJ transgene) mutants display an increased penetrance of the bypass phenotype (43% vs. 28%) relative to the corresponding Lar^13.2/Lar^5.5 transheterozygote.

(Fox and Zinn, 2005)

Expression of dlp^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^elav-C155, rescues the medulla patterning defects of Sdc¹⁰⁶⁰⁸/Df(2R)48 mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue the ERG defects.

(Rawson et al., 2005)

Xenogenetic Interactions

Statement

Reference

(Nguyen et al., 2016)

Complementation and Rescue Data

Fails to complement

Sdc^k10215

(BDGP Project Members, 1994-1999)

Rescued by

Sdc¹⁰⁶⁰⁸/Df(2R)48 is rescued by Scer\GAL4^elav.PU/Sdc^{UAS.FL.Tag:MYC}

(Nguyen et al., 2016)

Sdc¹⁰⁶⁰⁸/Df(2R)48 is rescued by Scer\GAL4^how-24B/Sdc^{UAS.FL.Tag:MYC}

(Nguyen et al., 2016)

Sdc¹⁰⁶⁰⁸/Df(2R)48 is rescued by Scer\GAL4^elav.PU/Sdc^UAS.cJa

(Nguyen et al., 2016)

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is rescued by Scer\GAL4^elav.PU/Sdc^UAS.cJa

(Johnson et al., 2006)

Partially rescued by

Sdc¹⁰⁶⁰⁸/Df(2R)48 is partially rescued by Scer\GAL4^how-24B/Sdc^UAS.cJa

(Nguyen et al., 2016)

Sdc¹⁰⁶⁰⁸ is partially rescued by Scer\GAL4^how-24B/Sdc^UAS.cJa

(Johnson et al., 2006)

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is partially rescued by Scer\GAL4^elav-C155/Sdc^UAS.cSa

(Rawson et al., 2005)

Not rescued by

Sdc¹⁰⁶⁰⁸/Df(2R)48 is not rescued by Scer\GAL4^how-24B/Sdc^{ΔCyto.UAS.Tag:MYC}

(Nguyen et al., 2016)

Sdc¹⁰⁶⁰⁸/Df(2R)48 is not rescued by Scer\GAL4^how-24B/Sdc^{ΔC1.UAS.Tag:MYC}

(Nguyen et al., 2016)

Sdc¹⁰⁶⁰⁸/Df(2R)48 is not rescued by Sdc^{ΔC2.UAS.Tag:MYC}/Scer\GAL4^how-24B

(Nguyen et al., 2016)

Sdc¹⁰⁶⁰⁸/Df(2R)48 is not rescued by Scer\GAL4^how-24B/Sdc^{ΔEcto.UAS.Tag:MYC}

(Nguyen et al., 2016)

Comments

Sdc¹⁰⁶⁰⁸/Df(2R)48 third instar larvae (in which Sara function has been rescued by Sara^Ubi.PJ) have a decrease in the number of boutons per neuromuscular junction, compared to controls; this phenotype is completely rescued by pre- (Scer\GAL4^elav.PU) or post- (Scer\GAL4^how-24B) synaptic expression of Sdc^{Scer\UAS.FL.T:Hsap\MYC}, or pre- (and partial rescue with post-) synaptic expression of Sdc^Scer\UAS.cJa; the phenotype is not rescued by expression of Sdc^{ΔCyto.Scer\UAS.T:Hsap\MYC}, Sdc^{ΔC1.Scer\UAS.T:Hsap\MYC}, Sdc^{ΔC2.Scer\UAS.T:Hsap\MYC} or Sdc^{ΔEcto.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^how-24B.

(Nguyen et al., 2016)

Expression of Sdc^Scer\UAS.cJa under the control of Scer\GAL4^elav.PU almost completely rescues the reduction in bouton number at the neuromuscular junction which is seen in Sdc¹⁰⁶⁰⁸/Df(2R)48 larvae (carrying Sara^Ubi.PJ to provide Sara function).

Expression of Sdc^Scer\UAS.cJa under the control of Scer\GAL4^how-24B provides limited rescue of the reduction in bouton number at the neuromuscular junction which is seen in homozygous Sdc¹⁰⁶⁰⁸ larvae.

(Johnson et al., 2006)

Expression of Sdc^Scer\UAS.cSa, under the control of Scer\GAL4^elav-C155, rescues the medulla cartridge crossover and R7/R8 termini disruption pupal phenotypes of Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue photoreceptor projection defects to the larval lamina or ERG abnormalities in adults.

(Rawson et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: Fkbp13⁰⁰⁷³⁴. Complements: MESK2⁰¹⁴⁶⁷. Complements: dom^k08108. Complements: l(2)k10317^k10317.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

P2377

(Nguyen et al., 2016)

Sdc¹⁰⁶⁰⁸

(Cho et al., 2012, De Luca et al., 2010, Kucherenko et al., 2008)

Sdc^P2377

(Johnson et al., 2006)

Sdc^P

(Johnson et al., 2006)

Sdc^l(2)10608

(Johnson et al., 2006)

l(2)10608

(Lincecum et al., 2000, Spradling et al., 1999)

l(2)10608¹⁰⁶⁰⁸

(BDGP Project Members, 1994-1999)

sdc¹⁰⁶⁰⁸

(Thuveson et al., 2019, Ren et al., 2009, Johnson et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (17)

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