FlyBase Allele Report: Dmel\nompC[3]

General Information

Symbol

Dmel\nompC³

Species

D. melanogaster

Name

FlyBase ID

FBal0008344

Feature type

allele

Associated gene

Dmel\nompC

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Nucleotide substitution: A2860T.

(Walker et al., 2000)

Amino acid replacement: K747term.

(Walker et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:5,357,976..5,357,976 [+]

Nucleotide change:

A5357976T

Reported nucleotide change:

A2860T

Amino acid change:

Reported amino acid change:

K747term

(Walker et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception (with nompC¹)

(Zhang et al., 2013)

abnormal behavior

(Walker et al., 2000)

abnormal chemosensory behavior | adult stage (with nompC^f00642)

(Sánchez-Alcañiz et al., 2017)

abnormal locomotor behavior | adult stage (with nompC¹)

(Gong et al., 2013, Cheng et al., 2010)

abnormal locomotor behavior | larval stage

(Cheng et al., 2010)

abnormal locomotor behavior | larval stage (with nompC¹)

(Cheng et al., 2010)

abnormal neurophysiology

(Walker et al., 2000)

abnormal neurophysiology (with nompC¹)

(Yan et al., 2013)

abnormal neurophysiology | adult stage (with nompC¹)

(Sánchez-Alcañiz et al., 2017)

abnormal neurophysiology | adult stage (with nompC^f00642)

(Sánchez-Alcañiz et al., 2017)

abnormal neurophysiology | adult stage | conditional

(Zhou et al., 2019)

abnormal neurophysiology | third instar larval stage (with nompC¹)

(Zhang et al., 2015)

abnormal touch perception (with nompC¹)

(Yan et al., 2013)

abnormal touch perception | larval stage (with nompC¹)

(Yan et al., 2018)

abnormal touch perception | third instar larval stage (with nompC¹)

(Zhang et al., 2015)

abnormal touch response (with nompC¹)

(Yan et al., 2013)

abnormal touch response | larval stage (with nompC¹)

(Yan et al., 2018)

abnormal touch response | third instar larval stage (with nompC¹)

(Zhang et al., 2015)

decreased rate of movement | larval stage (with nompC¹)

(Zhang et al., 2014)

lethal - all die before end of larval stage

(Cheng et al., 2010)

short lived (with nompC¹)

(Sánchez-Alcañiz et al., 2017)

some die during third instar larval stage

(Cheng et al., 2010)

uncoordinated

(Walker et al., 2000)

uncoordinated | adult stage (with nompC¹)

(Sánchez-Alcañiz et al., 2017, Gong et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

nompC³/nompC^f00642 mutant adult females have no difference in preference for softer plain, or 100 mM sucrose-containing, agarose for egg laying, compared to controls.

(Wu et al., 2019)

nompC³ mutant adults have significantly reduced spiking and mechanotransduction currents of mechanosensory neurons of the labellar taste bristles and labellar taste pegs in response to mechanical stimuli, compared to controls; there is no effect on depolarization of bristle mechanosensory neurons in response to current injections, compared to controls.

(Zhou et al., 2019)

nompC¹/nompC³ mutant larvae show a significantly decreased response to gentle touch, as compared to controls.

(Yan et al., 2018)

nompC³/nompC^f00642 starved adult males have significantly reduced preference for softer sucrose-containing agarose, compared to controls; these transheterozygotes do not have significant alterations in exploratory behavior or sucrose preference, compared to controls.

nompC³/nompC^f00642 adults have significant reductions in activity (spiking) of the L2 and L3 labellar sensilla in response sucrose stimulation and significant reductions in activity (spiking) of the L3, but not L2, labellar sensilla in response to mechanical stimulation, compared to controls; nompC¹/nompC³ adults have significant reductions in activity (spiking) of the L2 and L3 labellar sensilla in response mechanical stimulation and significant reductions in activity (spiking) of the L2, but not L3, labellar sensilla in response to sucrose stimulation, compared to controls; nompC¹/nompC³ adults also have a severe lack of coordination and die soon after hatching.

(Sánchez-Alcañiz et al., 2017)

nompC¹/nompC³ class III da neurons do not respond electrophysiologically to mechanical stimulation, and third instar larvae have a reduced response to gentle touch.

(Zhang et al., 2015)

nompC¹/nompC³ mutant larvae have a significant increase in defecation interval compared to controls.

(Zhang et al., 2014)

nompC¹/nompC³ flies show severely uncoordinated movements and very low locomotion speed.

(Gong et al., 2013)

nompC¹/nompC³ mutant larvae show defects in the behavioural response to gentle touch. These larvae also show less of an increase in action potential frequency and a reduced increase in internal calcium levels in response to the stimulus.

(Yan et al., 2013)

Cho neurons in nompC¹/nompC³ mutants do not respond to a low level of sound that is sufficient to trigger a Ca[2+] response in Cho neurons of wild-type larvae; however, a higher level of sound stimulation elicits a response, although this is still weaker than in controls.

(Zhang et al., 2013)

Most homozygotes die at the third instar stage.

nompC¹/nompC³ larvae show a prolonged stride duration (the average time needed to complete a single peristaltic contraction cycle) with normal stride size when their locomotion is compared to wild-type controls.

Crawling speed of homozygous and nompC¹/nompC³ larvae is reduced compared to controls.

nompC¹/nompC³ adults show severe defects in walking behaviour.

(Cheng et al., 2010)

nompC²/nompC³ third instar larvae show the same preference for 17.5[o]C over 14[o]C in a two-way choice test as do wild-type controls.

(Kwon et al., 2010)

nompC³/nompC⁴ mutants retain a preference for 18[o]C (i.e. are thermotactic).

(Kwon et al., 2008)

Flies show severe uncoordination. Mechanoreceptor currents are reduced to 10% of normal. Action potentials are almost completely abolished.

(Walker et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

nompC¹/nompC³ has abnormal touch response | larval stage phenotype, non-enhanceable by Atat^KO/Atat^KO

(Yan et al., 2018)

nompC¹/nompC³ has abnormal touch perception | larval stage phenotype, non-enhanceable by Atat^KO/Atat^KO

(Yan et al., 2018)

Enhancer of

Statement

Reference

nompC¹/nompC³ is an enhancer of abnormal touch response | larval stage phenotype of Atat^KO

(Yan et al., 2018)

nompC¹/nompC³ is an enhancer of abnormal touch perception | larval stage phenotype of Atat^KO

(Yan et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nompC³ is rescued by nompC^lexAop.L.GFP/Ecol\lexA^p65.nompC

(Zhou et al., 2019)

nompC¹/nompC³ is rescued by Scer\GAL4^19-12/nompC^L.UAS.GFP

(Zhang et al., 2015)

nompC¹/nompC³ is rescued by Scer\GAL4^nompC.PC/nompC^L.UAS

(Gong et al., 2013)

nompC¹/nompC³ is rescued by Scer\GAL4^nompC.PC/nompC^E1511A.UAS

(Gong et al., 2013)

nompC¹/nompC³ is rescued by Scer\GAL4^19-12/nompC^L.UAS

(Yan et al., 2013)

nompC¹/nompC³ is rescued by nompC^+tC

(Zhang et al., 2013)

nompC¹/nompC³ is rescued by Scer\GAL4^nompC.PC/nompC^L.UAS

(Cheng et al., 2010)

nompC¹/nompC³ is rescued by Scer\GAL4^5-40/nompC^L.UAS

(Cheng et al., 2010)

nompC¹/nompC³ is rescued by Scer\GAL4^21-7/nompC^L.UAS

(Cheng et al., 2010)

nompC¹/nompC³ is rescued by nompC^L.UAS/Scer\GAL4²²¹

(Cheng et al., 2010)

nompC¹/nompC³ is rescued by nompC^L.UAS.GFP/Scer\GAL4²²¹

(Cheng et al., 2010)

nompC³ is rescued by nompC^+tC

(Walker et al., 2000)

Partially rescued by

nompC¹/nompC³ is partially rescued by Scer\GAL4^19-12/nompC^{29+29ARs.UAS.EGFP}

(Zhang et al., 2015)

nompC¹/nompC³ is partially rescued by Scer\GAL4^nompC.PC/nompC^D1516A.UAS

(Gong et al., 2013)

Not rescued by

nompC¹/nompC³ is not rescued by Scer\GAL4^19-12/nompC^{Δ29ANK.UAS.GFP}

(Zhang et al., 2015)

nompC¹/nompC³ is not rescued by nompC^{Δ12ANK.UAS.GFP}/Scer\GAL4^19-12

(Zhang et al., 2015)

nompC¹/nompC³ is not rescued by Scer\GAL4^19-12/nompC^{29+17ARs.UAS.EGFP}

(Zhang et al., 2015)

Comments

Expression of nompC^{L.Scer\UAS.T:Avic\GFP} (but not nompC^{Δ29ANK.Scer\UAS.T:Avic\GFP}, nompC^{Δ12ANK.Scer\UAS.T:Avic\GFP} or nompC^{29+17ARs.Scer\UAS.T:Avic\GFP-EGFP}) driven by Scer\GAL4^19-12 rescues the electrophysiological and locomotor deficits in response to touch of nompC¹/nompC³ third instar larvae; expression of nompC^{29+29ARs.Scer\UAS.T:Avic\GFP-EGFP} leads to partial rescue.

(Zhang et al., 2015)

Expression of either nompC^L.Scer\UAS or nompC^{E1511A.Scer\UAS} under the control of Scer\GAL4^nompC.PC rescues the reduced locomotion of nompC¹/nompC³ adults.

Expression of nompC^{D1516A.Scer\UAS} under the control of Scer\GAL4^nompC.PC partially but significantly rescues the reduced locomotion of nompC¹/nompC³ adults.

(Gong et al., 2013)

Expression of nompC^L.Scer\UAS under the control of Scer\GAL4^19-12 rescues the touch sensitivity defects seen in nompC¹/nompC³ mutant larvae. The defects in behavior, action potential frequency and calcium response are all rescued.

(Yan et al., 2013)

The lethality of nompC¹/nompC³ larvae is rescued by expression of nompC^L.Scer\UAS under the control of Scer\GAL4^nompC.PC. The reduced crawling speed and increased stride duration seen in nompC¹/nompC³ larvae is also rescued.

The reduced crawling speed and increased stride duration of nompC¹/nompC³ larvae is rescued by expression of nompC^L.Scer\UAS under the control of either Scer\GAL4^5-40, Scer\GAL4^21-7 or Scer\GAL4^2-21.

The walking defects seen in nompC¹/nompC³ adults are rescued by expression of nompC^L.Scer\UAS under the control of either Scer\GAL4^21-7 or Scer\GAL4^nompC.PC.

The reduced crawling speed of nompC¹/nompC³ larvae is rescued by expression of nompC^{L.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^2-21.

(Cheng et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Due to a naming clash that goes back many years, two different alleles of nompC, with contrasting molecular lesions, were named nompC[3]. Thanks to user input, we have been able to disambiguate these two alleles. They are now called nompC[3] and nompC[b19]. The nompC[3] allele was generated in the Zuker lab by EMS (FBrf0127378). The nompC[b19] allele was made by Szidonya and Reuter (FBrf0047784) and published in 1988 as jf24[b19]. Allele jf24[b19] was renamed in Lindsley and Zimm 1992 (p. 353) as l(2)25Dc[3]. Allele l(2)25Dc[3] subsequently became nompC[3] based on a foot note in Kernan et al., 1994 (FBrf0073546) identifying l(2)25Dc as nompC. The references and data for jf24[b19]/l(2)25Dc[3]/nompC[3] have now been split from the nompC[3] FlyBase record and are associated with allele nompC[b19].

(FlyBase, 2009-)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

NompC³

(Luedke et al., 2024, Yan et al., 2013)

l(2)25Dc³

l(2)jf24^b19

Name Synonyms

Secondary FlyBase IDs

References (26)

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