FlyBase Allele Report: Dmel\Cul3[gft2]

General Information

Symbol

Dmel\Cul3^gft2

Species

D. melanogaster

Name

FlyBase ID

FBal0008511

Feature type

allele

Associated gene

Dmel\Cul3

Associated Insertion(s)

Carried in Construct

Also Known As

Cul-3^gft2, gft^HG39

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Ou et al., 2002)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Roote, 1995.5.4)

Progenitor genotype

Cytology

Description

5bp deletion at nucleotide positions 2310-2314, resulting in a frameshift at amino acid 747 and a premature stop codon at amino acid 748.

(Mistry et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:15,266,454..15,266,458 [-]

Comment:

5bp deletion resulting in a frameshift at amino acid 747 and a premature stop codon at amino acid 748. Position of mutation on reference sequence inferred by FlyBase curator.

(Mistry et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Zhu et al., 2005)

lethal | germline clone | maternal effect | recessive

(Mistry et al., 2004)

lethal | larval stage | recessive

(Mistry et al., 2004)

lethal | recessive

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna)

lethal - all die before end of larval stage (with Df(2L)TE35D-15)

(Mistry et al., 2004)

lethal - all die during embryonic stage | recessive

(Ashburner et al., 1990)

male sterile (with Cul3^mds1)

(Roote, 2008.8.22, Arama et al., 2007, Arama et al., 2007)

male sterile (with Cul3^mds2)

(Arama et al., 2007)

male sterile (with Cul3^mds3)

(Arama et al., 2007)

male sterile (with Cul3^mds4)

(Arama et al., 2007)

male sterile (with Cul3^mds5)

(Arama et al., 2007)

some die during embryonic stage | recessive

(Ashburner et al., 1990)

some die during larval stage (with Df(2L)TE35D-15)

(Mistry et al., 2004)

visible | recessive | somatic clone

(Mistry et al., 2004)

Phenotype Manifest In

actin cytoskeleton | adult stage | female

(Hudson et al., 2015)

campaniform sensillum | ectopic | somatic clone

(Mistry et al., 2004)

campaniform sensillum of dorsal radius | ectopic | somatic clone

(Mistry et al., 2004)

dorsal appendage | germline clone | maternal effect

(Mistry et al., 2004)

egg | germline clone | maternal effect

(Mistry et al., 2004)

external sensory organ | ectopic | somatic clone

(Mistry et al., 2004)

filamentous actin

(Hudson and Cooley, 2010)

macrochaeta | somatic clone

(Mistry et al., 2004)

microchaeta | somatic clone

(Mistry et al., 2004)

neuron & mushroom body

(Zhu et al., 2005)

nurse cell ring canal

(Hudson and Cooley, 2010)

nurse cell ring canal | germline clone

(Hudson et al., 2015)

sensory mother cell | cell autonomous | ectopic | somatic clone

(Mistry et al., 2004)

spermatid (with Cul3^mds1)

(Arama et al., 2007)

trichogen cell | ectopic | somatic clone

(Mistry et al., 2004)

wing | somatic clone

(Mistry et al., 2004)

wing disc | somatic clone

(Mistry et al., 2004)

wing hair | somatic clone

(Mistry et al., 2004)

wing vein | somatic clone

(Mistry et al., 2004)

wing vein L3 | somatic clone

(Mistry et al., 2004)

Detailed Description

Statement

Reference

Cul3^gft2 mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).

(Neukomm et al., 2017)

Cul3^gft2 heterozygotes do not display any obvious dominant defects in the number of primordial germ cells in the early embryo (i.e. mitotic cycles 12/13), as compared to controls.

(Pae et al., 2017)

Germline clones of cells homozygous mutant for Cul3^gft2 show a ring canal phenotype in the egg chambers: the F-actin cytoskeleton is disorganized, the kel protein accumulates in the lumen and the lumen diameter is significantly smaller compared to controls.

(Hudson et al., 2015)

Examination of Cul-3^gft2 mutant ring canals in egg chambers reveals a small lumen phenotype and F-actin disorganization.

(Hudson and Cooley, 2010)

cul-3^mds1/cul-3^gft2 spermatids show defects in individualisation.

(Arama et al., 2007)

Homozygous mutant gft² Mushroom Body clones exhibit axon terminal morphogenetic defects.

(Zhu et al., 2005)

Homozygous and gft²/Df(2L)TE35D-15 animals hatch, but die during the second larval instar. Females carrying homozygous germ-line clones produce few eggs and those which are laid are about half the size of wild-type and have fused dorsal appendages. No discernible embryonic structure can be detected interior to the chorion in these eggs. Large homozygous clones in the wing show a number of defects, including defects in the overall shape of the wing and in formation and position of the wing veins. Large clones in the wing often lack trichomes in the centre of the clone. Clones that cover the L3 vein often result in it shifting posteriorly. Clones that cover wing veins are associated with loss of vein tissue. Clones in the wing also result in ectopic sensory organ formation; clones that encompass a large part of the L3 vein have ectopic campaniform sensilla, ectopic campaniform sensilla also arise between wing vein L2 and the anterior wing margin and ectopic bristles arise distally between L2 and L3. Clones in the notum are associated with significant tufting of both micro- and macrochaetae, with the tufts being made up of ectopic fully formed external sensory organs as well as individual sensory organs that contain multiple shafts within a single socket. Homozygous clones in the wing disc show a dramatic increase in the number of sensory organ precursor (SOP) cells, while SOP development outside the clone is normal.

(Mistry et al., 2004)

Adult escapers have unexpanded wings and small eyes.

(Ashburner et al., 1990)

gft², but not other alleles, fails to

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Cul3^gft2 is an enhancer of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

cul-3[+]/Cul3^gft2 is an enhancer of visible phenotype of KLHL18^UAS.cFNa, Scer\GAL4^pnr-MD237

(Fujiyama-Nakamura et al., 2009)

Suppressor of

Statement

Reference

Cul3^gft2 is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Cul3[+]/Cul3^gft2 is a suppressor | partially of female sterile | dominant phenotype of gcl^NLS

(Pae et al., 2017)

Cul3[+]/Cul3^gft2 is a suppressor of decreased occurrence of cell division | somatic clone | larval stage phenotype of COX5A^tend

(Mandal et al., 2010)

gft[+]/Cul3^gft2 is a suppressor of visible phenotype of Gαs^Q215L.UAS, Scer\GAL4^OK10

(Mistry et al., 2004)

NOT Suppressor of

Statement

Reference

Cul3^gft2 is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Other

Statement

Reference

Cul3[+]/Cul3^gft2, gcl^rev390 has decreased cell number | embryonic stage phenotype

(Pae et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

cul-3[+]/Cul3^gft2 is an enhancer of adult external thorax | dorsal phenotype of KLHL18^UAS.cFNa, Scer\GAL4^pnr-MD237

(Fujiyama-Nakamura et al., 2009)

Suppressor of

Statement

Reference

Cul3^gft2 is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Cul3[+]/Cul3^gft2 is a suppressor | partially of egg chamber phenotype of gcl^NLS

(Pae et al., 2017)

Cul-3[+]/Cul3^gft2 is a suppressor of eye | somatic clone phenotype of COX5A^tend

(Mandal et al., 2010)

NOT Suppressor of

Statement

Reference

Cul3^gft2 is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Cul3^gft2 is a non-suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Other

Statement

Reference

Cul3[+]/Cul3^gft2, gcl^rev390 has primordial germ cell | embryonic stage phenotype

(Pae et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The axon degeneration in sensory neurons clones in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^VGlut-OK371 can be suppressed by combination with Cul3^gft2.

(Neukomm et al., 2017)

Cul3^gft2, gcl^rev390 double heterozygotes present significant decreases in the number of primordial germ cells in the early embryo (i.e. mitotic cycles 12/13), as compared to wild-type and single heterozygous controls.

Heterozygosity for Cul3^gft2 partially rescues the failure of gcl^NLS heterozygous females to develop late stage egg chambers and to lay eggs.

(Pae et al., 2017)

A Cul-3^gft2 heterozygous background does not suppress the disruptions in cell division seen in CoVa^tend mutant eye disc clones.

(Mandal et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Cul3^mds5

(Arama et al., 2007)

Cul3^mds4

(Arama et al., 2007)

Cul3^mds3

(Arama et al., 2007)

Cul3^mds2

(Arama et al., 2007)

Cul3^gft8

(Zhu et al., 2005)

Rescued by

Cul3^gft2 is rescued by Scer\GAL80^αTub84B.PL/Cul3^UASp.Venus/Scer\GAL4^αTub84B.PL

(Hudson and Cooley, 2010)

Comments

Ring canal morphology in egg chambers is rescued as a result of the expression of Cul-3^{Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL80^αTub84B.PL and Scer\GAL4^αTub84B.PL.

(Hudson and Cooley, 2010)

complement In(2LR)Sco^rv1.

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23866

Cul3^gft2 P{neoFRT}40A/CyO

Kyoto

106883

Adhⁿ¹⁰ Cul3^gft2 cn¹ vg¹/CyO

Notes on Origin

Discoverer

Harrington.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

Cul-3^gft2

(Ribot et al., 2022, Strutt et al., 2013, Grima et al., 2012, Hudson and Cooley, 2010)

Cul3²

(Zhu et al., 2005)

Cul3^GFT2

(Wang et al., 2023)

Cul3^HG39

(Hudson et al., 2015)

Cul3^gft2

(Ou et al., 2002)

Cul3^Δ

(Wang et al., 2023)

HG39

(Roote, 1995.5.4, Ashburner et al., 1983)

cul-3^gft2

cul3^gft2

(Neukomm et al., 2017, Pae et al., 2017, Zhou et al., 2015, Liu et al., 2014, Walker et al., 2013, Wong et al., 2013, Djagaeva and Doronkin, 2009, Arama et al., 2007, Arama et al., 2007, Zhang et al., 2006)

cul3^gft

(Kaplan et al., 2010)

gft²

(Fujiyama-Nakamura et al., 2009, Roote, 2008.8.22)

gft^HG39

(Mandal et al., 2010, Mistry et al., 2004)

l(2)^HG39

(Ashburner et al., 1990)

l(2)br34^HG39

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Ashburner et al., 1982)

Name Synonyms

Secondary FlyBase IDs

References (31)

Report Sections

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