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General Information
Symbol
Dmel\Dp49Fk-1
Species
D. melanogaster
Name
FlyBase ID
FBal0009081
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dDPvr10
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G13226235A

Amino acid change:

R217H | Dp-PA; R213H | Dp-PB; R189H | Dp-PC

Comment:

site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: R217H.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

One copy of Dp49Fk-1 is unable to suppress position effect variegation (PEV) at the w locus caused by In(1)wm4.

One copy of Dp49Fk-1 is unable to suppress the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

Females carrying homozygous germline clones are sterile. Eggs show a range of different chorion phenotypes. 17% of eggs have two somewhat normal dorsal appendages, 80% have a single fused appendage located at the dorsal midline, and 3% have either misplaced appendages or lack appendages all together. 99% of the eggs do not develop to a stage when a cuticle is produced; they are either never fertilised or do not develop sufficiently that a cuticle is secreted. Of the small number of embryos that do develop a cuticle, several show severe but variable pattern defects, while others appear normal. Occasionally a larva hatches from an egg laid by females carrying homozygous germline clones. Young homozygous egg chambers (in females carrying homozygous germline clones) show an aberrant accumulation of actin in several brightly stained foci, which persist throughout oogenesis. Actin bundles do form in mutant nurse cells in late stage egg chambers, but their density is reduced compared to wild type. Mutant nurse cells fail to degenerate on schedule. Approximately 10% of mutant egg chambers contain approximately double the normal number of nurse cell nuclei.

Semilethal over Df(2R)vg56 and Df(2R)vg33 at 25[o]C. Dp49Fk-1/Df(2R)vg56 and Dp49Fk-1/Df(2R)vg33 flies do not eclose at 29[o]C. Homozygous stage 13 to 14 embryos incorporate much less BrdU in a 15 minute pulse labeling than wild-type embryos. S phase is longer than in wild-type embryos in cells of the anterior midgut.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

Dp49Fk-1 is a non-enhancer of visible phenotype of upd1GMR.PB

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Dp49Fk-1 is a non-suppressor of visible phenotype of upd1GMR.PB

Dp49Fk-1 is a non-suppressor of visible phenotype of DpGMR.PD, E2f1GMR.PD

Phenotype Manifest In
Enhancer of
Statement
Reference

Dp49Fk-1 is an enhancer of eye phenotype of CycEJP

NOT Enhancer of
Statement
Reference

Dp49Fk-1 is a non-enhancer of eye phenotype of upd1GMR.PB

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Dp49Fk-1 is a non-suppressor of eye phenotype of upd1GMR.PB

Additional Comments
Genetic Interactions
Statement
Reference

Heterozygosity for Dp49Fk-1 suppresses the dopaminergic neuronal phenotypes in animals expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF.

Heterozygosity for Dp49Fk-1 suppresses the dopaminergic neuronal phenotypes of homozygous let-7Δ.cSa animals.

A DpKG00660 ; Dp49Fk-1 mutant background enhances the moderately rough eye phenotype observed upon expression of mblC.Scer\UAS under the control of Scer\GAL4hs.2sev.

Dominantly enhances the rough eye phenotype of CycEJP homozygotes.

Xenogenetic Interactions
Statement
Reference

Dp49Fk-1 enhances the eye phenotypes seen when Mmus\Shisa5Scer\UAS.cGa is expressed under the control of Scer\GAL4dpp.PH.

Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments

Dp+tgDPwt rescues the viability of Dp49Fk-1/Df(2R)BSC272 mutants.

Although DpgDPmut rescues the viability of Dp49Fk-1/Df(2R)BSC272 mutants, the rescued animals show a loss of dopaminergic neurons and locomotor defects.

The two major defects seen in females carrying homozygous Dp49Fk-1 germline clones (loss of dorsal-ventral polarity in the egg and a failure to correctly form a 16-cell cyst) are significantly rescued by Dp+mMa.

Dp49Fk-1/Df(2R)vg56 and Dp49Fk-1/Df(2R)vg33 flies failure to eclose at 29oC is partially rescued by Dphs.PD.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Lasko.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
References (12)