FlyBase Allele Report: Dmel\l(2)gl[1]

General Information

Symbol

Dmel\l(2)gl¹

Species

D. melanogaster

Name

FlyBase ID

FBal0009222

Feature type

allele

Associated gene

Dmel\l(2)gl

Associated Insertion(s)

Carried in Construct

Also Known As

lgl¹

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Mechler et al., 1985)

Progenitor genotype

Cytology

Description

Deletion of sequences from 0 to 19kb (coordinates with respect to proximal end of a cloned l(2)gl region, close to the tip of 2L).

(Mechler et al., 1985)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Betschinger et al., 2006)

CEA with l(2)gl⁴

(Betschinger et al., 2006)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | third instar larval stage

(Betschinger et al., 2006)

abnormal neuroanatomy

(Betschinger et al., 2006)

abnormal neuroanatomy (with l(2)gl⁴)

(Wirtz-Peitz et al., 2008)

abnormal size | third instar larval stage (with l(2)gl⁴)

(Goh et al., 2013)

lethal - all die before end of larval stage

some die during larval stage

Phenotype Manifest In

adult optic lobe

brain | third instar larval stage (with l(2)gl⁴)

(Goh et al., 2013)

embryonic/larval optic lobe

(Nedelcheva et al., 2001)

epithelial cell | extended germ band stage | germline clone | maternal effect

(Hutterer et al., 2004)

imaginal disc

(Nedelcheva et al., 2001)

larval brain

(Nedelcheva et al., 2001)

larval brain | larval stage (with l(2)gl⁴)

(Wirtz-Peitz et al., 2008)

larval brain | posterior & neuroblast | supernumerary

(Betschinger et al., 2006)

larval brain | posterior & neuron

(Betschinger et al., 2006)

larval neuroblast (with l(2)gl⁴)

(Wirtz-Peitz et al., 2008)

Detailed Description

Statement

Reference

The brains of l(2)gl¹/l(2)gl⁴ mutant third instar larvae are enlarged.

(Goh et al., 2013)

l(2)gl¹/l(2)gl⁴ larval brain neuroblasts show overproliferation.

(Wirtz-Peitz et al., 2008)

l(2)gl¹ mutants show an overproliferation of central brain neuroblasts in the posterior half of the brain and a concomitant reduction in the amount of neurons. The proliferation begins in the mid-third larval instar and increases dramatically during the late-third instar. Neuroblasts in the anterior half of the brain appear normal in number and spacing.

(Betschinger et al., 2006)

By the extended germband stages, the epithelia of embryos from l(2)gl¹ germ-line clone mothers loose their regular arrangement as epithelial cells round up.

(Hutterer et al., 2004)

The brain-ventral ganglion complexes of mutant larvae over 5 days old have brain hemispheres which are abnormal in size and morphology. Neuroblastomas of the optic lobes of the brain ganglia and tumourous imaginal discs are present in the mutant larvae.

(Nedelcheva et al., 2001)

90% of mutants die as larvae, 2% as pupae. Extreme allele: larval brain and optic lobes become enlarged and disorganised and the imaginal discs are large and clumped. Discs of mutant larvae transplanted into wild-type female abdomens form large contained tumours, whereas transplanted optic primordia from larval brains form invasive neuroblastomas, which grow rapidly, killing the host within 7-14 days. These tumours can be serially cultured in adult abdomens.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

l(2)gl¹/l(2)gl⁴ has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^insc-Mz1407/baz^UASp.GFP

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has abnormal neuroanatomy phenotype, enhanceable by numb[+]/numb¹⁵

(Wirtz-Peitz et al., 2008)

Suppressed by

Statement

Reference

l(2)gl¹/l(2)gl⁴ has abnormal size | third instar larval stage phenotype, suppressible by park¹

(Goh et al., 2013)

l(2)gl¹/l(2)gl⁴ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^insc-Mz1407/baz^GD1384

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has abnormal neuroanatomy phenotype, suppressible | partially by numb[+]/numb^S52F

(Wirtz-Peitz et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

l(2)gl¹/l(2)gl⁴ has larval neuroblast phenotype, enhanceable by Scer\GAL4^insc-Mz1407/baz^UASp.GFP

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has larval brain | larval stage phenotype, enhanceable by Scer\GAL4^insc-Mz1407/baz^UASp.GFP

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has larval neuroblast phenotype, enhanceable by numb[+]/numb¹⁵

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has larval brain | larval stage phenotype, enhanceable by numb[+]/numb¹⁵

(Wirtz-Peitz et al., 2008)

Suppressed by

Statement

Reference

l(2)gl¹/l(2)gl⁴ has brain | third instar larval stage phenotype, suppressible by park¹

(Goh et al., 2013)

l(2)gl¹/l(2)gl⁴ has larval neuroblast phenotype, suppressible by Scer\GAL4^insc-Mz1407/baz^GD1384

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has larval brain | larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/baz^GD1384

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has larval neuroblast phenotype, suppressible | partially by numb[+]/numb^S52F

(Wirtz-Peitz et al., 2008)

l(2)gl¹/l(2)gl⁴ has larval brain | larval stage phenotype, suppressible | partially by numb[+]/numb^S52F

(Wirtz-Peitz et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

park¹ suppresses the enlarged brain phenotype seen in l(2)gl¹/l(2)gl⁴ mutant third instar larvae.

(Goh et al., 2013)

The overproliferation of larval brain neuroblasts seen in l(2)gl¹/l(2)gl⁴ animals is enhanced by expression of baz^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4¹⁴⁰⁷.

The overproliferation of larval brain neuroblasts seen in l(2)gl¹/l(2)gl⁴ animals is completely suppressed by expression of baz^GD1384 under the control of Scer\GAL4¹⁴⁰⁷.

The overproliferation of larval brain neuroblasts seen in l(2)gl¹/l(2)gl⁴ animals is enhanced by numb¹⁵/+ and is weakly suppressed by numb^S52F/+.

(Wirtz-Peitz et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

l(2)gl¹ is rescued by Scer\GAL4^pros.PMG/l(2)gl^UAS.cBa

(Betschinger et al., 2005)

l(2)gl¹ is rescued by Scer\GAL4^da.G32/l(2)gl^UAS.Tag:MYC

(Hutterer et al., 2004)

Not rescued by

l(2)gl¹ is not rescued by Scer\GAL4^pros.PMG/l(2)gl^{MC.UAS.Tag:MYC}

(Betschinger et al., 2005)

l(2)gl¹ is not rescued by Scer\GAL4^pros.PMG/l(2)gl^{C+β.UAS.Tag:MYC}

(Betschinger et al., 2005)

l(2)gl¹ is not rescued by l(2)gl^{M.UAS.Tag:MYC}/Scer\GAL4^pros.PMG

(Betschinger et al., 2005)

l(2)gl¹ is not rescued by Scer\GAL4^pros.PMG/l(2)gl^{N.UAS.Tag:MYC}

(Betschinger et al., 2005)

l(2)gl¹ is not rescued by Scer\GAL4^da.G32/l(2)gl^{3A.UAS.Tag:MYC}

(Hutterer et al., 2004)

Comments

l(2)gl^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^da.G32 rescues the epithelial polarity defects seen in embryos from l(2)gl¹ germ-line clone mothers but l(2)gl^{3A.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^da.G32 fails to rescue this phenotype.

(Hutterer et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Bridges, 9th May 1933.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

l(2)gl¹

lgl¹

(Goh et al., 2013, Song and Lu, 2012, Wirtz-Peitz et al., 2008, Wirtz-Peitz et al., 2008, Betschinger et al., 2006, Betschinger et al., 2005, Mayer et al., 2005, Hutterer et al., 2004, Ohshiro et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

Open Close