FlyBase Allele Report: Dmel\spi[s3547]

General Information

Symbol

Dmel\spi^s3547

Species

D. melanogaster

Name

FlyBase ID

FBal0009312

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

P{lacW}spi^s3547

Carried in Construct

Also Known As

spi-lacZ, s3547

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}spi^s3547

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

ameliorates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.GMR

(Read et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Dworkin and Gibson, 2006, Spradling et al., 1999)

lethal - all die during embryonic stage | recessive

(Perrimon et al., 1996)

some die during embryonic stage | recessive

(Perrimon et al., 1996)

visible | dominant

(Dworkin and Gibson, 2006)

Phenotype Manifest In

embryonic/first instar larval cuticle

(Perrimon et al., 1996)

embryonic/larval ganglionic tracheal branch

(Gallio et al., 2004)

larval ventral nerve cord

(Gallio et al., 2004)

ventral denticle belt | embryonic stage

(Perrimon et al., 1996)

wing | anterior

(Dworkin and Gibson, 2006)

Detailed Description

Statement

Reference

Flies heterozygous for spi^s3547 have normal cardiac function.

(Yu et al., 2010)

Heterozygotes show a direct effect on the shape of the wing, having an effect in the anterior region, but no effect in the posterior region.

(Dworkin and Gibson, 2006)

spi^s3547 embryos have severe defects in migration of the ganglionic branch trachea during stage 16, with many branches crossing the midline. The ventral nerve cord is also defective in these embryos, with longitudinal fascicles collapsed onto the midline.

(Gallio et al., 2004)

Homozygous embryos exhibit deletion of median parts of all denticle belts.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

spi^s3547/spi[+] is an enhancer of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

NOT Enhancer of

Statement

Reference

spi^s3547 is a non-enhancer of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

spi^s3547 is a non-enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

spi^s3547 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

spi^s3547 is a suppressor of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

spi^s3547 is a non-suppressor of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

spi^s3547 is a non-suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

spi^s3547/spi[+] is an enhancer of eye phenotype of Egfr^E1

(Read et al., 2005)

NOT Enhancer of

Statement

Reference

spi^s3547 is a non-enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

spi^s3547 is a non-enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

spi^s3547 is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

spi^s3547 is a suppressor of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

spi^s3547 is a non-suppressor of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

spi^s3547 is a non-suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

spi^unspecified

(Dworkin and Gibson, 2006)

spi⁰¹⁰⁶⁸

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10462

w¹¹¹⁸; P{lacW}spi^s3547/CyO

Kyoto

114338

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{lacW}spi^s3547 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

M. Scott.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

l(2)01068^s3547

l(2)s3547

(Spradling et al., 1999)

l(2)s3547^s3547

s3547

(Dworkin et al., 2011, Dworkin and Gibson, 2006)

spi^S3547

(Zhu et al., 2017, Armstrong et al., 2005)

spi^s3547

(Xu et al., 2011, Fetting et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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