FlyBase Allele Report: Dmel\Alg3[tid1]

General Information

Symbol

Dmel\Alg3^tid1

Species

D. melanogaster

Name

FlyBase ID

FBal0009359

Feature type

allele

Associated gene

Dmel\Alg3

Associated Insertion(s)

Carried in Construct

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

2R:23,705,941..23,705,941 [-]

Nucleotide change:

C23705941T

Amino acid change:

Q375term | Alg3-PA

Reported amino acid change:

Q375term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(de Vreede et al., 2018)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: Q375term.

(de Vreede et al., 2018)

A nonsense mutation at glutamine 375, resulting in a truncation of the remaining 135 amino acids of the predicted Alg3 protein.

(de Vreede et al., 2018)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

cell shape defective | larval stage (with Alg3^tid2)

(de Vreede et al., 2018)

decreased occurrence of cell division | larval stage (with Alg3^tid2)

(de Vreede et al., 2018)

developmental rate defective | larval stage (with Alg3^tid2)

(de Vreede et al., 2018)

increased cell number | larval stage (with Alg3^tid2)

(de Vreede et al., 2018)

large body | larval stage (with Alg3^tid2)

(de Vreede et al., 2018)

lethal

(de Vreede et al., 2018)

lethal | recessive

(Kurzik-Dumke et al., 1995)

lethal - all die before end of pupal stage | somatic clone

(de Vreede et al., 2018)

tumorigenic

(Gateff and Mechler, 1989)

tumorigenic | larval stage (with Alg3^tid2)

(de Vreede et al., 2018)

visible | larval stage (with Alg3^tid2)

(de Vreede et al., 2018)

Phenotype Manifest In

eye disc (with Alg3^tid2)

(de Vreede et al., 2018)

eye disc | somatic clone

(de Vreede et al., 2018)

imaginal disc

(Kurzik-Dumke et al., 1995)

wing disc

(Canamasas et al., 2003)

wing disc (with Alg3^tid2)

(de Vreede et al., 2018)

Detailed Description

Statement

Reference

Alg3^tid1/Alg3^tid2 transheterozygotes develop into giant larvae that bear imaginal disc tumors and show delayed puparium formation, as compared to controls. Imaginal disc proliferation rate in Alg3^tid1/Alg3^tid2 transheterozygotes is slow, such that discs are initially smaller than controls; eventually, eye and wing discs show disorganized tissue architecture (abnormal tissue thickness and tissue folding, cells with altered shape but apparently normal cell polarity) and moderate overgrowth associated with a significant increase in cell number, as compared to controls; xenografts into the abdomen of control hosts continue proliferating and overgrow dramatically, with more severe architectural defects, as compared to controls.

The generation of fully homozygous Alg3^tid1 eye discs in an otherwise heterozygous larva leads to disorganized eye discs (milder defects than those in Alg3^tid1/Alg3^tid2 transheterozygotes) and induces pupal lethality.

(de Vreede et al., 2018)

Mutants have tumorous wing discs.

(Canamasas et al., 2003)

Shows autonomously growing imaginal disc tumors and lethality during the larval to pupal transition.

(Kurzik-Dumke et al., 1995)

Mutant imaginal discs implanted into wild-type flies proliferate as non-invasive tumours that kill the host in 10 to 14 days. Pieces of mutant imaginal disc implanted into larvae give rise to chitinous structures containing some hairs but no bristles in the adult fly.

(Gateff and Mechler, 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, enhanceable by egr^UAS.cIa/Scer\GAL4^r4

(de Vreede et al., 2018)

NOT Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-enhanceable by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-enhanceable by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-enhanceable by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-enhanceable by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, suppressible by hpo^UAS.cPa/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/bsk^K53R.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/grnd^KK109939

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, suppressible | partially by grnd^extra.UAS/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, suppressible by egr^GD12658/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, suppressible by egr^GD12658/Scer\GAL4^r4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, suppressible by wts^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

NOT suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/wgn^dsRNA.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by Scer\GAL4^Hml.Δ/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has tumorigenic | larval stage phenotype, non-suppressible by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Other

Statement

Reference

Alg3^tid2/Alg3^tid1, Scer\GAL4^r4, egr^UAS.Venus has increased cell death phenotype

(de Vreede et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, enhanceable by egr^UAS.cIa/Scer\GAL4^r4

(de Vreede et al., 2018)

NOT Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by hpo^UAS.cPa/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/bsk^K53R.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/grnd^KK109939

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by grnd^extra.UAS/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible by egr^GD12658/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible by egr^GD12658/Scer\GAL4^r4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by wts^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

NOT suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/wgn^dsRNA.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^Hml.Δ/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Other

Statement

Reference

Alg3^tid2/Alg3^tid1, Scer\GAL4^r4, egr^UAS.Venus has wing disc phenotype

(de Vreede et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of egr^UAS.Venus under the control of Scer\GAL4^r4 in a Alg3^tid1/Alg3^tid2 transheterozygous background leads to an increase in cell death (DCP-1-positive cells) in the wing disc, as compared to controls.

(de Vreede et al., 2018)

Alg3^tid1 ptc^tuf-1 double homozygous larvae develop tumourous imaginal discs and die at the larval/pupal transition, just as Alg3^tid1 homozygotes do. The phenotype of Alg3^tid1 ptc⁹ double mutant embryos resembles that of ptc⁹ single mutants.

(Canamasas et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Alg3^tid2/Alg3^tid1 is rescued by Alg3^UAS.cVa/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid1 is rescued by Alg3^UAS.cVa/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 is rescued by Alg3^Tub.cVa

(de Vreede et al., 2018)