FlyBase Allele Report: Dmel\Alg3[tid2]

General Information

Symbol

Dmel\Alg3^tid2

Species

D. melanogaster

Name

FlyBase ID

FBal0009360

Feature type

allele

Associated gene

Dmel\Alg3

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

Progenitor genotype

Cytology

Description

A 24 bp deletion and a single base pair insertion, inducing an in-frame 24bp deletion that removes amino acids 36-43, and a 1 bp frameshift leading to premature termination of the Alg3 protein after 192 amino acids.

(de Vreede et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2R:23,709,585..23,709,608 [-]

Comment:

Inferred boundaries of a 24bp in-frame deletion that is accompanied by a 1 base insertion. This leads to a frameshift and early translation termination of the Alg3 protein after 192 amino acids.

Inserted_sequence:

(de Vreede et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

X95249

CAA64538

UniProtKB/Swiss-Prot

Q27237

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of benign neoplasm

CEA with Ras85D^V12.UAS

(de Vreede et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | larval stage (with Alg3^tid1)

(de Vreede et al., 2018)

abnormal developmental rate | larval stage (with Alg3^tid1)

(de Vreede et al., 2018)

abnormal size | larval stage | somatic clone

(de Vreede et al., 2018)

decreased cell number | larval stage | somatic clone

(de Vreede et al., 2018)

decreased occurrence of cell division | larval stage (with Alg3^tid1)

(de Vreede et al., 2018)

increased body size | larval stage (with Alg3^tid1)

(de Vreede et al., 2018)

increased cell number | larval stage (with Alg3^tid1)

(de Vreede et al., 2018)

lethal

(de Vreede et al., 2018)

lethal | recessive

(Kurzik-Dumke et al., 1995)

lethal - all die before end of pupal stage | somatic clone

(de Vreede et al., 2018)

neoplasia | larval stage (with Alg3^tid1)

(de Vreede et al., 2018)

visible | larval stage (with Alg3^tid1)

(de Vreede et al., 2018)

Phenotype Manifest In

eye disc (with Alg3^tid1)

(de Vreede et al., 2018)

eye disc | somatic clone

(de Vreede et al., 2018)

imaginal disc

(Kurzik-Dumke et al., 1995)

wing disc (with Alg3^tid1)

(de Vreede et al., 2018)

Detailed Description

Statement

Reference

The generation of fully homozygous Alg3^tid2 eye discs in an otherwise heterozygous larva leads to disorganized eye discs (milder defects than those in Alg3^tid1/Alg3^tid2 transheterozygotes) and induces pupal lethality.

Alg3^tid2 homozygous clones within mosaic eye discs are smaller and formed by less cells than control clones, indicating that they are outcompeted.

(de Vreede et al., 2018)

Shows autonomously growing imaginal disc tumors and lethality during the larval to pupal transition.

(Kurzik-Dumke et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, enhanceable by egr^UAS.cIa/Scer\GAL4^r4

(de Vreede et al., 2018)

NOT Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-enhanceable by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-enhanceable by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-enhanceable by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-enhanceable by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, suppressible by wts^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, suppressible by hpo^UAS.cPa/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/bsk^K53R.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/grnd^KK109939

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, suppressible | partially by grnd^extra.UAS/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, suppressible by egr^GD12658/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, suppressible by egr^GD12658/Scer\GAL4^r4

(de Vreede et al., 2018)

NOT suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/wgn^RNAi.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by Scer\GAL4^Hml.Δ/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has neoplasia | larval stage phenotype, non-suppressible by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Other

Statement

Reference

Alg3^tid2, Ras85D^V12.UAS, Scer\GAL4^unspecified has neoplasia | somatic clone | larval stage phenotype

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1, Scer\GAL4^r4, egr^UAS.Venus has increased cell death phenotype

(de Vreede et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, enhanceable by egr^UAS.cIa/Scer\GAL4^r4

(de Vreede et al., 2018)

NOT Enhanced by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-enhanceable by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by wts^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by hpo^UAS.cPa/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/bsk^K53R.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/grnd^KK109939

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible | partially by grnd^extra.UAS/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible by egr^GD12658/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, suppressible by egr^GD12658/Scer\GAL4^r4

(de Vreede et al., 2018)

NOT suppressed by

Statement

Reference

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Xbp1^GD4745/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/Xbp1^HMS03015

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/sip3^EY11980

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by sip3^EY11980/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/wgn^RNAi.UAS

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^hh-Gal4/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by Scer\GAL4^Hml.Δ/egr^GD12658

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by crb^HMS02036/Scer\GAL4^hh-Gal4

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 has wing disc phenotype, non-suppressible by aPKC^GL00007/Scer\GAL4^Bx-MS1096

(de Vreede et al., 2018)

Other

Statement

Reference

Alg3^tid2, Ras85D^V12.UAS, Scer\GAL4^unspecified has eye disc | somatic clone phenotype

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1, Scer\GAL4^r4, egr^UAS.Venus has wing disc phenotype

(de Vreede et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Eye disc clones both homozygous for Alg3^tid2 and expressing Ras85D^V12.UAS under the control of Scer\GAL4^unspecified show non-invasive overgrowths, as compared to either single mutant clones.

(de Vreede et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Alg3^tid2/Alg3^tid1 is rescued by Alg3^UAS.cVa/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid2/Alg3^tid1 is rescued by Alg3^Tub.cVa

(de Vreede et al., 2018)

Alg3^tid2 is rescued by Alg3^UAS.cVa/Scer\GAL4^αTub84B.PL

(de Vreede et al., 2018)

Alg3^tid2 is rescued by Alg3^Tub.cVa

(de Vreede et al., 2018)

Alg3^tid2 is rescued by CG5504^+t7.0

(Kurzik-Dumke et al., 1995)

Partially rescued by

Alg3^tid2/Alg3^tid1 is partially rescued by Scer\GAL4^hh-Gal4/Alg3^UAS.cVa

(de Vreede et al., 2018)

Not rescued by

Alg3^tid2/Alg3^tid1 is not rescued by Alg3^UAS.cVa/Scer\GAL4^r4

(de Vreede et al., 2018)

Alg3^tid2 is not rescued by CG5504^tKa

(Kurzik-Dumke et al., 1995)

Alg3^tid2 is not rescued by CG5504^tKb

(Kurzik-Dumke et al., 1995)

Comments

Tumorous phenotype can be rescued by Alg3^+t7.0.

(Kurzik-Dumke et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

90941

w^*; P{neoFRT}42D Alg3^tid2 Kr^If-1/CyO, P{Ubi-GFP.S65T}PAD1

Notes on Origin

Discoverer

Induced in: the "b If" strain.

(Kurzik-Dumke et al., 1998)

External Crossreferences and Linkouts ( 3 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

X95249

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

CAA64538

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q27237

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Alg3^tid2

alg3^tid2

(de Vreede et al., 2018)

l(2)tid²

(Kurzik-Dumke et al., 1992)

tid²

(de Vreede et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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