Allele Dmel\Fer2LCH⁰⁰⁰³⁵

General Information
Symbol	Dmel\Fer2LCH⁰⁰⁰³⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0009364
Feature type	allele	Associated gene	Dmel\Fer2LCH
Also Known As	l(3)00035⁰⁰⁰³⁵

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:26,215,015..26,215,015 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Mendes et al., 2009, Li, 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion within the open reading frame. (Missirlis et al., 2007)
Caused by insertion	P{PZ}Fer2LCH⁰⁰⁰³⁵ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Mendes et al., 2009, Li, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective \| first instar larval stage (Li, 2010) lethal \| first instar larval stage (Li, 2010) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999, Missirlis et al., 2007)
Phenotype Manifest In

Detailed Description
	Statement Reference The lethality seen in homozygous or hemizygous Fer2LCH[00035] mutants cannot be rescued by the addition of iron. Fer2LCH[00035] mutants grow to mid-first instar after hatching and then stop growing. They are arrested at this stage but survive for approximately 7 days before dying. Fer2LCH[00035] mutant larvae show diminished BrdU incorporation and triacylglycerol levels 18hrs after hatching, as found in starved wild-type flies. (Li, 2010) l(3)00035/l(3)10605 and l(3)00035/l(3)00451: thin bristles and semi-sterile: may be weak alleles
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement Reference Fer2LCH⁰⁰⁰³⁵ has lethal \| recessive phenotype, non-suppressible by Scer\GAL4^Act.PU/Fer1HCH^mut.Scer\UAS (Missirlis et al., 2007) Fer2LCH⁰⁰⁰³⁵ has lethal \| recessive phenotype, non-suppressible by Scer\GAL4^Act.PU/Fer1HCH^Scer\UAS.cMa (Missirlis et al., 2007)
Phenotype Manifest In
Enhancer of
Statement Reference Fer2LCH[+]/Fer2LCH⁰⁰⁰³⁵ is an enhancer of scutellar bristle \| supernumerary phenotype of Ssdp^L7 (Bronstein et al., 2010)
NOT Enhancer of
Statement Reference Fer2LCH[+]/Fer2LCH⁰⁰⁰³⁵ is a non-enhancer of scutellar bristle \| supernumerary phenotype of Chi^e5.5 (Bronstein et al., 2010) Fer2LCH[+]/Fer2LCH⁰⁰⁰³⁵ is a non-enhancer of wing margin phenotype of Bx² (Bronstein et al., 2010)
NOT Suppressor of
Statement Reference Fer2LCH[+]/Fer2LCH⁰⁰⁰³⁵ is a non-suppressor of scutellar bristle \| supernumerary phenotype of Chi^e5.5 (Bronstein et al., 2010) Fer2LCH[+]/Fer2LCH⁰⁰⁰³⁵ is a non-suppressor of wing margin phenotype of Bx² (Bronstein et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Fer2LCH⁰⁰⁸²³ (BDGP Project Members, 1994-1999) Fer2LCH⁰²⁵⁴⁵ (BDGP Project Members, 1994-1999) Fer2LCH⁰⁴⁰⁵⁰ (BDGP Project Members, 1994-1999) Fer2LCH⁰⁴⁰⁹⁵ (BDGP Project Members, 1994-1999) Fer2LCH⁰⁴²⁴⁴ (BDGP Project Members, 1994-1999) Fer2LCH⁰⁶⁷⁰¹ (BDGP Project Members, 1994-1999) Fer2LCH⁰⁷⁰¹⁶ (BDGP Project Members, 1994-1999) Fer2LCH⁰⁷⁰⁷⁸ (BDGP Project Members, 1994-1999) Fer2LCH^j2A3 (BDGP Project Members, 1994-1999) Fer2LCH^s2083 (BDGP Project Members, 1994-1999) Fer2LCH^s2696b (BDGP Project Members, 1994-1999)
Rescued by	Fer2LCH⁰⁰⁰³⁵ is rescued by Scer\GAL4^Act.PU/Fer2LCH^Scer\UAS.cMa (Missirlis et al., 2007)
Comments	Ubiquitous expression of Fer2LCH[Scer\UAS.cLa] under the control of Scer\GAL4[Act5C.PI] rescues the first instar larval stage lethality seen in Fer2LCH[00035] mutants. (Li, 2010)
Stocks ( 1 )
Bloomington	11483 ry⁵⁰⁶ P{PZ}Fer2LCH⁰⁰⁰³⁵/TM3, ry^RK Sb¹ Ser¹
Notes on Origin
Discoverer	A. Spradling.

Comments
	Complements: Fer1HCH⁰⁰⁴⁵¹. Complements: tmod⁰⁰⁸⁴⁸. Complements: Fer1HCH⁰²⁰³⁸. Complements: Fer1HCH⁰⁴⁶⁹³. Complements: Fer1HCH⁰⁹⁴⁰⁸. Complements: Fer1HCH¹⁰⁶⁰⁵. Complements: Fer1HCH^j10B4. Complements: l(3)j2D5^j2D5. Complements: l(3)s2564^s2564. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	Fer2LCH⁰⁰⁰³⁵ (Christensen et al., 2008.4.15, Bronstein et al., 2010, Li, 2010) Fer2LCH³⁵ (Missirlis et al., 2007) fer2lch⁰³⁵ (Mendes et al., 2009) l(3)00035 (Spradling et al., 1999) l(3)00035⁰⁰⁰³⁵ (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, )
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Bronstein et al., 2010, PLoS Genet. 6(8): e1001063 Transcriptional regulation by CHIP/LDB complexes. [FBrf0211594] Li, 2010, Cell Res. 20(10): 1148--1157 Identification of iron-loaded ferritin as an essential mitogen for cell proliferation and postembryonic development in Drosophila. [FBrf0211953] Mendes et al., 2009, EMBO J. 28(9): 1296--1307 ER stress protects from retinal degeneration. [FBrf0207956] Missirlis et al., 2007, Genetics 177(1): 89--100 Homeostatic mechanisms for iron storage revealed by genetic manipulations and live imaging of Drosophila ferritin. [FBrf0202180] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Personal communication to FlyBase	Christensen et al., 2008.4.15, Isolation and characterization of Df(3R)BSC503. Isolation and characterization of Df(3R)BSC503. [FBrf0204531] Cook, 2006.1.31, Characterization of deletions and Minutes in chromosomal region 99-100. Characterization of deletions and Minutes in chromosomal region 99-100. [FBrf0191633] Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\Fer2LCH00035

Allele Dmel\Fer2LCH⁰⁰⁰³⁵