FlyBase Allele Report: Dmel\Hmgcr[01152]

General Information

Symbol

Dmel\Hmgcr⁰¹¹⁵²

Species

D. melanogaster

Name

FlyBase ID

FBal0009402

Feature type

allele

Associated gene

Dmel\Hmgcr

Associated Insertion(s)

P{PZ}Hmgcr⁰¹¹⁵²

Carried in Construct

Also Known As

l(3)01152

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Treisman and Rubin, 1996)

Progenitor genotype

Associated Insertion(s)

P{PZ}Hmgcr⁰¹¹⁵²

Cytology

Description

Insertion in the first exon of Hmgcr.

(Yi et al., 2006)

Insertion in the first exon (within the 5' untranslated region).

(Yi, 2006)

P{PZ} is inserted 179 nucleotides into the 5' end of the Hmgcr transcript.

(Van Doren et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:23,733,493..23,733,493 [+]

(Yi, 2006, Yi et al., 2006, Spradling et al., 1999, Russell et al., 1998, Van Doren et al., 1998, Treisman and Rubin, 1996, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal vessel wall cell

(Yi et al., 2006)

dorsal vessel wall cell | embryonic stage 17

(Yi, 2006)

embryonic/first instar larval cuticle

(Russell et al., 1998, Perrimon et al., 1996)

embryonic/first instar larval cuticle | rescuable maternal effect (with Hmgcr^11.57)

(Deshpande and Schedl, 2005)

embryonic dorsal vessel (with Df(3R)Exel9013)

(Yi et al., 2006)

embryonic pericardial cell

(Yi et al., 2006)

embryonic pericardial cell | embryonic stage 17

(Yi, 2006)

filzkorper | rescuable maternal effect (with Hmgcr^11.57)

(Deshpande and Schedl, 2005)

germline cell

(Van Doren and Lehmann, 2001.2.14)

mouthpart | embryonic stage | rescuable maternal effect (with Hmgcr^11.57)

(Deshpande and Schedl, 2005)

primordial germ cell

(Santos and Lehmann, 2004)

primordial germ cell | embryonic stage (with Hmgcr^11.54)

(Barton et al., 2024)

ventral denticle belt | rescuable maternal effect (with Hmgcr^11.57)

(Deshpande and Schedl, 2005)

Detailed Description

Statement

Reference

Homozygous stage 17 embryos show a "broken hearted" phenotype; the pericardial cells are dissociated from cardioblasts in the dorsal vessel.

Hmgcr⁰¹¹⁵²/Df(3R)Exel9013 mutants show distortion of the shape of the dorsal vessel.

(Yi et al., 2006)

Cardioblasts and pericardial cells are properly specified and aligned until stage 16, but in later embryos the cardioblasts and pericardial cells begain to dissociate from each other.

(Yi, 2006)

Embryos whose mothers carry gemline clones homozygous for Hmgcr⁰¹¹⁵² and whose fathers are Hmgcr^11.57/+ often have severe cuticle defects including disrupted denticle belts, abnormal mouthparts and incomplete filzkorper, but others have normal cuticles or mild fusion of abdominal segments 8 and 9. This latter group are probably paternally rescued.

(Deshpande and Schedl, 2005)

In Hmgcr⁰¹¹⁵² homozygous embryos an average of 8 germ cells fail to migrate properly.

(Santos and Lehmann, 2004)

Weak germ cell migration defect.

(Van Doren and Lehmann, 2001.2.14)

Homozygous embryos derived from females with Hmgcr⁺ activity do not have cuticle defects. Embryos derived from homozygous female germ line clones have cuticle defects, which can be partially paternally rescued.

(Russell et al., 1998)

Homozygous embryos do not exhibit cuticle defects. Germline clones produce eggs with poor cuticle development.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Hmgcr⁰¹¹⁵² has primordial germ cell phenotype, enhanceable by Fpps^k06103

(Santos and Lehmann, 2004)

Hmgcr⁰¹¹⁵² has primordial germ cell phenotype, enhanceable by qm^L14.4

(Santos and Lehmann, 2004)

Enhancer of

Statement

Reference

Hmgcr⁰¹¹⁵² is an enhancer of primordial germ cell phenotype of Fpps^k06103

(Santos and Lehmann, 2004)

Hmgcr⁰¹¹⁵² is an enhancer of primordial germ cell phenotype of qm^L14.4

(Santos and Lehmann, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

About 40% of Fpps^k06103; Hmgcr⁰¹¹⁵² double homozygous embryoshave severe patterning defects. However, in the remaining 60% the mesoderm appears correctly patterned but germ cell migration is severely defective (on average 14 germ cells remain on the gut), an enhancement over the germ cell migration phenotype seen in either single mutant. A similar degree of enhancement is seen in the presence of qm^L14.4/qm^L14.4.

(Santos and Lehmann, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Hmgcr⁰¹¹⁵² is rescued by Scer\GAL4^HCH.Hand/Hmgcr^UAS.cvDa

(Yi et al., 2006)

Hmgcr⁰¹¹⁵² is rescued by Scer\GAL4^Mef2.PR/Hmgcr^UAS.cvDa

(Yi et al., 2006)

Not rescued by

Hmgcr⁰¹¹⁵² is not rescued by Scer\GAL4^Ugt36A1.PK/Hmgcr^UAS.cvDa

(Yi et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Comments

Probably a hypomorph.

(Van Doren and Lehmann, 2001.2.14)

Complements: l(3)04684⁰⁴⁶⁸⁴.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

HMGCR⁰¹¹⁵²

(Yi, 2006)

Hmgcr⁰¹¹⁵²

(Barton et al., 2024, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15)

hmgcr¹¹⁵²²

(Deshpande and Schedl, 2005, Deshpande and Schedl, 2005)

l(3)01152

(Yi, 2006, Yi et al., 2006, Spradling et al., 1999, Russell et al., 1998)

l(3)01152⁰¹¹⁵²

Name Synonyms

Secondary FlyBase IDs

FBal0098376

References (16)

Report Sections

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