Allele Dmel\sqz⁰²¹⁰²

General Information
Symbol	Dmel\sqz⁰²¹⁰²	Species	D. melanogaster
Name		FlyBase ID	FBal0009434
Feature type	allele	Associated gene	Dmel\sqz
Also Known As	l(3)02102⁰²¹⁰², sqz^lacZ, l(3)02102

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:14,991,598..14,991,598 (BDGP Project Members, 1994-1999, Allan et al., 2003, McGovern et al., 2003, Russell et al., 1998, Spradling et al., 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{PZ}sqz⁰²¹⁰² (BDGP Project Members, 1994-1999, Allan et al., 2003, McGovern et al., 2003, Russell et al., 1998, Spradling et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Perrimon et al., 1996) lethal (with Df(3R)Dl-KX23) (Allan et al., 2003, Allan et al., 2003) lethal \| larval stage \| recessive (Russell et al., 1998, Perrimon et al., 1996) lethal \| partially (with Df(3R)Dl-KX23) (Allan et al., 2003) lethal \| recessive (Spradling et al., 1999) locomotor behavior defective (with Df(3R)Dl-KX23) (Allan et al., 2003, Allan et al., 2003) neuroanatomy defective \| embryonic stage (Tsuji et al., 2008)
Phenotype Manifest In
	dorsal appendage (Russell et al., 1998) dorsal appendage \| germline clone (Perrimon et al., 1996) EL neuron \| embryonic stage \| supernumerary (Tsuji et al., 2008) embryonic/larval muscle system (with Df(3R)Dl-KX23) (Allan et al., 2003, Allan et al., 2003) embryonic/larval neuron (with Df(3R)Dl-KX23) (Allan et al., 2003) neuroblast NB3-3 \| embryonic stage (Tsuji et al., 2008) ovariole \| germline clone (Perrimon et al., 1996) proboscis (with Df(3R)Dl-KX23) (Allan et al., 2003) Tv neuron \| first instar larval stage (Félix et al., 2007) ventral nerve cord (with Df(3R)Dl-KX23) (Allan et al., 2003) wing (with Df(3R)Dl-KX23) (Allan et al., 2003)
Detailed Description
	Statement Reference Mutants show a delay in thoracic neuroblast 3-3 (NB3-3T) entering quiescence, and a corresponding increase in the number of EL neurons. Despite the delay, NB3-3T eventually enters quiescence as no proliferating NBs are found in the thorax of newly hatched sqz[02102] larvae. (Tsuji et al., 2008) Marker analysis indicates that Tv neurons are absent from sqz⁰²¹⁰² homozygous first instar larvae. sqz⁰²¹⁰² somatic clones do not cause any visible phenotype in adult wings. (Félix et al., 2007) sqz⁰²¹⁰²/Df(3R)Dl-KX23 mutant larvae display a motility defect whereby the body wall musculature over-contracts radially during the peristaltic wave typical of larval motility, apparent as a 'squeezing' of the intestine. This phenotype is fully penetrant. In mutants a frequent failure (~1/3) of the Tv axon fails to innervate the dorsal neurohemal organs. (Allan et al., 2003) The sqz⁰²¹⁰²/Df(3R)Dl-KX23 combination show considerable lethality during pupal stages. Mutant larvae are lethargic, display an apparent loss of balance whereby larvae are often observed laying in their side and demonstrate a motility defect whereby the body wall musculature overcontracts during the peristaltic wave typical of insect larval motility. The motility phenotype is fully penetrant. Those animals that eventually eclose are grossly normal although a failure of wing inflation and proboscis defects are seen. However the eclosing adults are entirely immotile except for a persistent tremor. These adults die within 24 hours. (Allan et al., 2003) Homozygotes die during the first and second larval instar stages. Females carrying homozygous germ line clones lay few eggs, which have defective dorsal appendages. (Russell et al., 1998) Germline clones produce abnormal eggs due to abnormal oogenesis: few eggs are laid that exhibit defective dorsal appendages. (Perrimon et al., 1996)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	11555 ry⁵⁰⁶ P{PZ}sqz⁰²¹⁰²/TM3, ry^RK Sb¹ Ser¹
Notes on Origin
Discoverer	A. Spradling.

Comments
	Complements: l(3)03346⁰³³⁴⁶. Complements: l(3)06404⁰⁶⁴⁰⁴. Complements: cdi⁰⁷⁰¹³. Complements: nos^j3B6. Complements: l(3)j5A6^j4A3. Complements: nos^j4B6. Complements: l(3)j5A6^j5A6. Complements: l(3)j5A6^j7C8. Complements: l(3)s1922^s1922. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	l(3)02102 (Spradling et al., 1999, Russell et al., 1998) l(3)02102⁰²¹⁰² (Allan et al., 2003, Perrimon et al., 1996, BDGP Project Members, 1994-1999, ) sqz⁰²¹⁰² (Tsuji et al., 2008) sqz^lacZ02102 (Félix et al., 2007) sqz^lacZ (Allan et al., 2003, Félix et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Tsuji et al., 2008, Development 135(23): 3859--3869 Neuroblast entry into quiescence is regulated intrinsically by the combined action of spatial Hox proteins and temporal identity factors. [FBrf0206246] Félix et al., 2007, Development 134(10): 1845--1852 Nab controls the activity of the zinc-finger transcription factors Squeeze and Rotund in Drosophila development. [FBrf0200657] Allan et al., 2003, Cell 113(1): 73--86 Specification of neuropeptide cell identity by the integration of retrograde BMP signaling and a combinatorial transcription factor code. [FBrf0158766] McGovern et al., 2003, Mech. Dev. 120(10): 1193--1207 A targeted gain of function screen in the embryonic CNS of Drosophila. [FBrf0167840] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Russell et al., 1998, Genome 41(1): 7--13 Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142]
Supplementary material	Allan et al., 2003, Cell 113(1): Supplementary material: tables S1 - S5. [FBrf0161463]
Personal communication to FlyBase	Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\sqz02102

Allele Dmel\sqz⁰²¹⁰²