FlyBase Allele Report: Dmel\Hem[03335]

General Information

Symbol

Dmel\Hem⁰³³³⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0009462

Feature type

allele

Associated gene

Dmel\Hem

Associated Insertion(s)

P{PZ}Hem⁰³³³⁵

Carried in Construct

Also Known As

Kette⁰³³³⁵, l(3)03335

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Schenck et al., 2004)

Mutagen

P-element activity

(Baumgartner et al., 1995)

Progenitor genotype

Associated Insertion(s)

P{PZ}Hem⁰³³³⁵

Cytology

Description

Insertion of a P{PZ} element 39bp downstream of the putative transcription start site.

(Baumgartner et al., 1995)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:22,288,144..22,288,144 [+]

(Hummel et al., 2000, Spradling et al., 1999, Baumgartner et al., 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | dominant

(Schenck et al., 2004)

lethal | recessive

(Spradling et al., 1999, Baumgartner et al., 1995)

Phenotype Manifest In

axon

(Schenck et al., 2004)

larval intersegmental nerve

(Schenck et al., 2004)

neuromuscular junction & synapse

(Schenck et al., 2004)

Detailed Description

Statement

Reference

Mutant embryos show axon defects, including ectopic crossing of the midline by axons and ectopic branching of the intersegmental nerve. Heterozygous larvae have significantly shorter synapses at the neuromuscular junction (91.9 +/- 4.1 μm) compared to wild-type larvae (111.1 μm).

(Schenck et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hem[+]/Hem⁰³³³⁵ is a suppressor of abnormal neuroanatomy | dominant phenotype of Fmr1^unspecified

(Schenck et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hem[+]/Hem⁰³³³⁵ is a suppressor of neuromuscular junction & synapse phenotype of Fmr1^unspecified

(Schenck et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrown synapse phenotype seen at the neuromuscular junction of Fmr1^unspecified/+ larvae is suppressed by one copy of Hem⁰³³³⁵. The reduced synaptic length seen at the neuromuscular junction of Hem⁰³³³⁵/+ larvae is not modified by SCAR^Δ37/+.

(Schenck et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Hem^C3-20

(Hummel et al., 2000)

Hem^G1-37

(Hummel et al., 2000)

Hem^J1-70

(Hummel et al., 2000)