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General Information
Symbol
Dmel\msn03349
Species
D. melanogaster
Name
FlyBase ID
FBal0009465
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
msnl(3)03349, msn-lacZ
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{PZ}msn03349
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

eye photoreceptor cell & axon

eye photoreceptor cell & growth cone

photoreceptor cell & axon

photoreceptor cell | precursor & nucleus, with Scer\GAL4unspecified, msnDN.UAS

photoreceptor cell R1 & axon (with msn102)

photoreceptor cell R2 & axon (with msn102)

photoreceptor cell R3 & axon (with msn102)

photoreceptor cell R4 & axon (with msn102)

photoreceptor cell R5 & axon (with msn102)

photoreceptor cell R6 & axon (with msn102)

Detailed Description
Statement
Reference

When msnDN.Scer\UAS is expressed in the developing eye of wild-type animals R-cell precursors migrate as normal. When expressed in msn03349/+ animals a highly penetrant nuclear migration phenotype is seen.

Almost no msn03349 homozygous flies eclose and a large proportion of those that do have thorax closure defects.

Many R1-R6 growth cones fail to stop at the lamina layer in msn03349/msn102 larvae. Most msn03349 animals die at the late pupal stage. msn03349 third instar larvae have a mild defect in photoreceptor cell axonal projections. The lamina plexus is uneven. Only 5% of msn03349 animals expressing msnD160N.Scer\UAS under the control of Scer\GAL4elav.PLu survive to the pupal stage.

Lethality acts during the larval and pupal stages.

The photoreceptor cell growth cones are able to extend into the developing optic lobe in homozygous third instar larvae, but their innervation patterns within the lamina and medulla are altered compared to wild type. Gaps are seen in the lamina R1-R6 termination site and abnormal, large bundles of axons are seen in the medulla. Growth cone morphology is altered, although the photoreceptor cell growth cones are able to expand upon reaching the target. The severity of the phenotype is variable.

Germline clones produce eggs with patterning defects: embryos are twisted with variable cuticle differentiation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference

msn03349 is an enhancer of visible phenotype of RetMEN2B.GMR

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

msn03349 has eye photoreceptor cell & growth cone phenotype, enhanceable by dock13421

msn03349 has lamina phenotype, enhanceable by dock13421

Suppressed by
Statement
Reference

msn03349 has adult thorax phenotype, suppressible by stck[+]/stck18

Enhancer of
Statement
Reference

msn03349 is an enhancer of eye phenotype of RetMEN2B.GMR

Additional Comments
Genetic Interactions
Statement
Reference

Introduction of a single copy of stck18 into the homozygous msn03349 homozygous background increases the incidence of eclosion from approximately 0% to approximately 15% and also suppresses the msn03349 thorax closure defect.

Homozygosity for icsBG02577 increases the incidence of eclosion seen in msn03349 homozygotes from approximately 0.5% to approximately 3%, and completely suppresses the msn03349 thorax closure defects.

The phenotype is dominantly enhanced by dock13421; the R1-R6 termination site at the lamina becomes more disorganised and photoreceptor cell growth cones are much less expanded.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Complements: l(3)0162901629. Complements: l(3)0626406264. Complements: mRpL1210534. Complements: Prm10631. Complements: mRpL12j4B2. Complements: l(3)neo141. Complements: mRpL12rL424. Complements: l(3)s2383s2383.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (14)