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General Information
Symbol
Dmel\HDAC104556
Species
D. melanogaster
Name
FlyBase ID
FBal0009513
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
rpd304556, l(3)04556, rpd3P-UTR, P{PZ}Rpd304556
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

FlyBase curator comment: Authors of FBrf0111344 state that P-element insertion is 47bp from the transcription start site, however see FBrf0108900 for authoritative statement of lesion.

P{PZ} is inserted at nucleotide 47 in the 223bp long 5' UTR of Rpd3.

Insertion components
P{PZ}HDAC104556
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

HDAC104556 heterozygote adults have increased lifespan and increased survival under paraquat-induced oxidative stress, as compared to wild-type controls.

Rpd304556 germline clones exhibit an abundance of γ-His2Av foci and evidence of a repair defect.

Rpd304556/+ flies have the normal number of dorsocentral bristles.

Similarly to wild-type controls, Rpd304556/+ heterozygotes show an age-dependent increase in susceptibility to temperature-sensitive paralysis.

Heterozygotes have no effect on the position effect variegation of w seen in the In(1)wm4h chromosome.

Heterozygous males show a 33% extension of life-span. Heterozygous females show a 52% extension of life-span. Calorific restriction does not result in a further extension of life-span in Rpd304556 heterozygotes.

Heterozygotes raised on medium containing trichostatin A (TSA) do not show an increase in the rate of lethality or developmental delay compared to wild-type flies raised on medium containing TSA and in fact the lethality caused by TSA is weakly suppressed in heterozygotes compared to wild type. Heterozygotes raised on medium containing trichostatin A (TSA) have a notched wing phenotype, with the notches increasing in number and size with increasing TSA concentration.

HDAC104556 homozygotes are lethal; HDAC104556/HDAC1303, HDAC104556/HDAC1313, HDAC104556/HDAC1326 and HDAC104556/HDAC1328 transheterozygotes are lethal under normal conditions, but the rare adults eclose and survive to short-lived and sterile adults in uncrowded yeast-supplemented conditions. When raised under carefully maintained culture conditions transheterozygotes of the genotype HDAC104556/HDAC1303 adult males eclose at 40% the expected rate and females at 30% the expected rate. Adults are short lived and females produce few eggs, apparently unfertilised. HDAC104556 has no dominant effect on PEV. Phenotypes include strong suppression of variegation in In(1)wm4, severely notched wings, small, malformed, sometimes curved or duplicated bristles, alulae that are larger than normal, and a reduction in the number of sex combs.

Embryos from mosaic females containing Rpd304556 homozygous germ-line clones, exhibit a pair rule phenotype in more than 80% of the majority (>65%) that fail to hatch. Partial or complete fusion of adjacent denticle belts are seen, resulting in embryos with 5 to 8 thoracic and abdominal segments. In addition variable, often severe, defects are seen in the head skeleton.

Embryos derived from Rpd304556 germline clones exhibit a variable pair-rule phenotype. They are also reduced in size.

Embryos derived from homozygous female germ line clones show a weak maternal effect, having pair rule segment defects.

Germline clones produce eggs with patterning defects: variable pair-rule segmentation defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

HDAC104556, Snr1E1/Snr1[+] has visible | dominant phenotype, suppressible | partially by brm2

HDAC104556, Snr1R3/Snr1[+] has visible | dominant phenotype, suppressible | partially by brm2

Enhancer of
Statement
Reference

HDAC104556/Rpd3[+] is an enhancer of visible | dominant phenotype of hepCA

HDAC104556/Rpd3[+] is an enhancer of visible | dominant phenotype of Snr1E1

Suppressor of
Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

HDAC104556 has sex comb phenotype, non-enhanceable by esc10

HDAC104556 has sex comb phenotype, non-enhanceable by E(z)63

Suppressed by
Statement
Reference

HDAC104556, Snr1E1/Snr1[+] has wing vein | ectopic phenotype, suppressible | partially by brm2

HDAC104556, Snr1E1/Snr1[+] has wing phenotype, suppressible | partially by brm2

HDAC104556, Snr1R3/Snr1[+] has wing vein | ectopic phenotype, suppressible | partially by brm2

HDAC104556, Snr1R3/Snr1[+] has wing phenotype, suppressible | partially by brm2

NOT suppressed by
Statement
Reference

HDAC104556 has sex comb phenotype, non-suppressible by esc10

HDAC104556 has sex comb phenotype, non-suppressible by E(z)63

Enhancer of
Statement
Reference

HDAC104556/Rpd3[+] is an enhancer of retina phenotype of Hsap\ATXN182Q.UAS, Scer\GAL4GMR.PU

HDAC104556/Rpd3[+] is an enhancer of eye phenotype of Hsap\APPAβ42.GMR.Tag:SS(rPENK)

HDAC104556/Rpd3[+] is an enhancer of wing phenotype of hepCA

HDAC104556/Rpd3[+] is an enhancer of wing vein | ectopic phenotype of net1

HDAC104556/Rpd3[+] is an enhancer of wing vein | ectopic phenotype of Snr1E1

HDAC104556/Rpd3[+] is an enhancer of wing phenotype of Snr1E1

HDAC104556 is an enhancer of sex comb phenotype of Pc4

NOT Enhancer of
Statement
Reference

HDAC104556/Rpd3[+] is a non-enhancer of retina phenotype of Hsap\HTT128Q.1-336.UAS, Scer\GAL4GMR.PU

HDAC104556 is a non-enhancer of sex comb phenotype of esc10

HDAC104556 is a non-enhancer of sex comb phenotype of E(z)63

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

HDAC104556 is a non-suppressor of sex comb phenotype of esc10

HDAC104556 is a non-suppressor of sex comb phenotype of E(z)63

HDAC104556/Rpd3[+] is a non-suppressor of eye phenotype of Scer\GAL4hs.2sev, ttk69.UAS.Tag:HA

Other
Additional Comments
Genetic Interactions
Statement
Reference

The disorganised eye phenotype caused by expression of sensScer\UAS.cNa under the control of Scer\GAL4lz-gal4 is suppressed by Rpd304556.

Rpd304556/+ enhances the phenotype of pnrD1/+, resulting in extra dorsocentral sensory organs.

The severity of the mutant eye phenotype caused by expression of IswiK159R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PH is enhanced by Rpd304556.

Rpd304556/+ suppresses the rough-eye phenotype and increases the number of normal photoreceptors per ommatidium in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU.

6.1% of Rpd304556 mutant males expressing GugdsRNA.1.Scer\UAS under the control of Scer\GAL4dpp.blk1 develop melanotic masses in their heads.

Rpd304556 dominantly suppresses the eye tumour phenotype of flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH).

The severity of the thoracic cleft seen in chm14 pharate adults is suppressed by heterozygosity for Rpd304556.

Progeny from Bin1R7-18, Bin1R7-18/Rpd304556 mothers show high levels of lethality (>50%) with nearly all of the unhatched embryos, more than a third of the hatched larvae display head defects. Most are missing the labrum, epistomal sclerite and dorsal and ventral arms are reduced in size.

The lifespan of males expressing Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4DJ651 is significantly extended if they also carry Rpd304556. The lifespan of females expressing Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4DJ651 is significantly extended if they also carry Rpd304556.

At 29oC, Rpd304556/+ enhances the ectopic wing vein phenotype of Snr1E1/+; 61% have an ectopic vein emanating from the posterior crossvein and 39% have ectopic veins emanating from the posterior crossvein and posterior to the L5 longitudinal vein. Rpd304556/Snr1R3 double heterozygotes have wing vein defects; 32% have normal wings, 38% have an ectopic vein emanating from the posterior crossvein and 30% have ectopic veins emanating from the posterior crossvein and posterior to the L5 longitudinal vein. Rpd304556/brm2 double heterozygotes have a normal wing phenotype. The ectopic wing vein phenotype seen in Rpd304556/Snr1R3 and Rpd304556/Snr1E1 double heterozygotes at 29oC is partially suppressed by brm2/+.

Sir205327a does not show a dominant synthetic lethal interaction with Rpd304556.

The partial lethality due to runScer\UAS.cLa; Scer\GAL4nos.PG (3% viable) is partially suppressed by maternal heterozygosity for Rpd304556 (rescues to 31% viable).

The addition of Pc4 to Rpd304556 animals increases the average number of extra sex comb teeth per leg from 2.7 to 3.94. The addition of esc10 or E(z)63 to Rpd304556 animals has no effect on the extra sex comb phenotype.

The eye phenotype caused by expression of ttk69.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.2sev is not dramatically modulated by Rpd304556.

Eggs from Rpd304556/groE48 mothers exhibit an embryonic lethality significantly higher than from either groE48/+ or Rpd304556/+ mothers. Cuticles from over 70% of unhatched embryos show a bicaudal phenotype, including a duplicated posterior spiracle, and a mirror image duplication of the posterior abdominal segments in place of the normal anterior segments. Eggs from Rpd304556/Df(3R)Espl22 mothers exhibit an embryonic lethality significantly higher than from either Df(3R)Espl22/+ or Rpd304556/+ mothers. Cuticles from over 70% of unhatched embryos show a bicaudal phenotype, including a duplicated posterior spiracle, and a mirror image duplication of the posterior abdominal segments in place of the normal anterior segments.

The lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev is suppressed by Rpd304556.

Xenogenetic Interactions
Statement
Reference

One copy of Rpd304556 does not enhance the retinal phenotype seen when Hsap\HTT128Q.1-336.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

One copy of Rpd304556 enhances the retinal phenotype seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

The decreased lifespan of males expressing Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4DJ651 is significantly suppressed if they also carry HDAC104556. The decreased lifespan of females expressing Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4DJ651 is significantly suppressed if they also carry HDAC104556.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
References (46)